Desulfovibrio vulgaris (strain ATCC 29579 / DSM 644 / NCIMB 8303 / VKM B-1760 / Hildenborough)
Average proteome isoelectric point is 6.61
Get precalculated fractions of proteins
Acidic
pI < 6.8
6.8-7.4
pI > 7.4
Basic
All
Note: above files contain also dissociation constants (pKa)
Virtual 2D-PAGE plot for 3518 proteins (isoelectric point calculated using IPC2_protein)
Get csv file with sequences according to given criteria:
* You can choose from 21 different methods for calculating isoelectric point
Summary statistics related to proteome-wise predictions
Protein with the lowest isoelectric point:
>tr|Q72BE8|Q72BE8_DESVH 1-acyl-sn-glycerol-3-phosphate acyltransferase putative OS=Desulfovibrio vulgaris (strain ATCC 29579 / DSM 644 / NCIMB 8303 / VKM B-1760 / Hildenborough) OX=882 GN=DVU_1688 PE=4 SV=1
MM1 pKa = 7.94 ADD3 pKa = 3.69 IFDD6 pKa = 3.69 QASDD10 pKa = 3.89 YY11 pKa = 9.58 EE12 pKa = 4.46 AKK14 pKa = 10.56 DD15 pKa = 3.31 RR16 pKa = 11.84 EE17 pKa = 4.35 AAISAAAGRR26 pKa = 11.84 ATWSGPSPDD35 pKa = 4.62 IIDD38 pKa = 4.52 GVPCCAEE45 pKa = 4.04 CGEE48 pKa = 4.58 PLPEE52 pKa = 4.67 ARR54 pKa = 11.84 LKK56 pKa = 10.72 ALPGVGLCVDD66 pKa = 3.95 CAEE69 pKa = 4.31
Molecular weight: 7.1 kDa
Isoelectric point according different methods:
IPC2.protein.svr19 3.793
IPC2_protein 4.012
IPC_protein 3.91
Toseland 3.719
ProMoST 4.012
Dawson 3.897
Bjellqvist 4.139
Wikipedia 3.834
Rodwell 3.745
Grimsley 3.63
Solomon 3.872
Lehninger 3.834
Nozaki 4.024
DTASelect 4.202
Thurlkill 3.783
EMBOSS 3.834
Sillero 4.024
Patrickios 1.914
IPC_peptide 3.872
IPC2_peptide 3.999
IPC2.peptide.svr19 3.962
Protein with the highest isoelectric point:
>sp|Q72C59|GCSPA_DESVH Probable glycine dehydrogenase (decarboxylating) subunit 1 OS=Desulfovibrio vulgaris (strain ATCC 29579 / DSM 644 / NCIMB 8303 / VKM B-1760 / Hildenborough) OX=882 GN=gcvPA PE=1 SV=1
MM1 pKa = 7.66 SVLQVVAVGTKK12 pKa = 9.65 RR13 pKa = 11.84 VASGHH18 pKa = 6.68 PGQKK22 pKa = 10.36 ANPVSGGLRR31 pKa = 11.84 HH32 pKa = 6.23 QRR34 pKa = 11.84 RR35 pKa = 11.84 QRR37 pKa = 11.84 HH38 pKa = 4.64 RR39 pKa = 11.84 VGAGIGATGGSLSVSGAAGRR59 pKa = 11.84 QIWLL63 pKa = 3.6
Molecular weight: 6.42 kDa
Isoelectric point according different methods:
IPC2.protein.svr19 9.468
IPC2_protein 11.023
IPC_protein 12.618
Toseland 12.778
ProMoST 13.276
Dawson 12.778
Bjellqvist 12.778
Wikipedia 13.261
Rodwell 12.398
Grimsley 12.822
Solomon 13.276
Lehninger 13.173
Nozaki 12.778
DTASelect 12.778
Thurlkill 12.778
EMBOSS 13.276
Sillero 12.778
Patrickios 12.149
IPC_peptide 13.276
IPC2_peptide 12.266
IPC2.peptide.svr19 9.12
Peptides (in silico digests for buttom-up proteomics)
Below you can find
in silico digests of the whole proteome with Trypsin, Chymotrypsin, Trypsin+LysC, LysN, ArgC proteases suitable for different mass spec machines.
Try ESI
ChTry ESI
ArgC ESI
LysN ESI
TryLysC ESI
Try MALDI
ChTry MALDI
ArgC MALDI
LysN MALDI
TryLysC MALDI
Try LTQ
ChTry LTQ
ArgC LTQ
LysN LTQ
TryLysC LTQ
Try MSlow
ChTry MSlow
ArgC MSlow
LysN MSlow
TryLysC MSlow
Try MShigh
ChTry MShigh
ArgC MShigh
LysN MShigh
TryLysC MShigh
General Statistics
Number of major isoforms
Number of additional isoforms
Number of all proteins
Number of amino acids
Min. Seq. Length
Max. Seq. Length
Avg. Seq. Length
Avg. Mol. Weight
3518
0
3518
1075623
18
3038
305.7
33.34
Amino acid frequency
Ala
Cys
Asp
Glu
Phe
Gly
His
Ile
Lys
Leu
11.439 ± 0.065
1.472 ± 0.021
5.726 ± 0.038
5.902 ± 0.042
3.591 ± 0.03
8.624 ± 0.042
2.453 ± 0.025
4.42 ± 0.034
3.136 ± 0.04
10.375 ± 0.051
Met
Asn
Gln
Pro
Arg
Ser
Thr
Val
Trp
Tyr
2.778 ± 0.018
2.401 ± 0.025
5.237 ± 0.038
2.946 ± 0.025
7.586 ± 0.045
5.158 ± 0.032
5.476 ± 0.036
7.801 ± 0.039
1.202 ± 0.017
2.28 ± 0.021
Note: For amino acid frequency statistics the error has been estimated with the bootstraping (x100) at the protein level
Most of the basic statistics you can see at this page can be downloaded from this CSV file
For dipeptide frequency statistics click here