Heterobasidion partitivirus 12
Average proteome isoelectric point is 7.33
Get precalculated fractions of proteins
Acidic
pI < 6.8
6.8-7.4
pI > 7.4
Basic
All
Note: above files contain also dissociation constants (pKa)
Virtual 2D-PAGE plot for 2 proteins (isoelectric point calculated using IPC2_protein)
Get csv file with sequences according to given criteria:
* You can choose from 21 different methods for calculating isoelectric point
Summary statistics related to proteome-wise predictions
Protein with the lowest isoelectric point:
>tr|W8PR73|W8PR73_9VIRU Capsid protein OS=Heterobasidion partitivirus 12 OX=1469905 PE=4 SV=1
MM1 pKa = 7.82 SSLPDD6 pKa = 3.58 FASMNEE12 pKa = 4.04 SEE14 pKa = 4.06 RR15 pKa = 11.84 LKK17 pKa = 11.04 YY18 pKa = 10.54 FEE20 pKa = 4.48 EE21 pKa = 4.17 QQKK24 pKa = 9.61 KK25 pKa = 9.44 QKK27 pKa = 10.85 DD28 pKa = 3.68 LVDD31 pKa = 3.55 QSIKK35 pKa = 10.85 AKK37 pKa = 8.52 QTMSAMRR44 pKa = 11.84 VRR46 pKa = 11.84 PVFQQAIDD54 pKa = 3.93 GAKK57 pKa = 9.96 SASKK61 pKa = 10.64 SSAVPTKK68 pKa = 10.44 AVPPPPFSGTSASANNMLRR87 pKa = 11.84 LAFGEE92 pKa = 4.62 SPFRR96 pKa = 11.84 PKK98 pKa = 10.41 QRR100 pKa = 11.84 FGQNGFIPDD109 pKa = 3.69 SQFLFTLLSIMDD121 pKa = 3.79 MKK123 pKa = 9.28 MTSTRR128 pKa = 11.84 KK129 pKa = 9.58 FIEE132 pKa = 4.55 GAEE135 pKa = 4.24 FWSPLISQYY144 pKa = 11.59 YY145 pKa = 7.47 MAVLVFVQIFRR156 pKa = 11.84 AMHH159 pKa = 5.53 EE160 pKa = 4.35 AGLLSGEE167 pKa = 4.17 AADD170 pKa = 5.64 VYY172 pKa = 11.58 DD173 pKa = 4.91 LFCGHH178 pKa = 6.36 SPAISLASLPVPGPFLNLLAQYY200 pKa = 10.05 AVHH203 pKa = 6.68 IPHH206 pKa = 7.04 LVDD209 pKa = 3.84 MDD211 pKa = 5.15 NICPMIPNNLDD222 pKa = 3.24 TTNTNFYY229 pKa = 10.06 KK230 pKa = 10.82 FQGLCSNLLGRR241 pKa = 11.84 LPNVPYY247 pKa = 10.88 LLDD250 pKa = 3.8 QIALLRR256 pKa = 11.84 DD257 pKa = 3.65 NLVAAPVSIDD267 pKa = 3.18 VANQGRR273 pKa = 11.84 VLHH276 pKa = 5.81 RR277 pKa = 11.84 TMFGQALFAINAAPGNAAQWNTRR300 pKa = 11.84 VPYY303 pKa = 10.2 GAAVPDD309 pKa = 3.5 NAKK312 pKa = 10.73 YY313 pKa = 10.28 ISCDD317 pKa = 3.05 PASRR321 pKa = 11.84 HH322 pKa = 5.69 PFFLNRR328 pKa = 11.84 GLISQMTDD336 pKa = 2.74 YY337 pKa = 11.39 AALINVNTPAAHH349 pKa = 6.12 NAAAITPSWTQFLGLDD365 pKa = 3.44 NMPFFLQVVRR375 pKa = 11.84 IMSWYY380 pKa = 9.16 SKK382 pKa = 9.33 FWQGSTDD389 pKa = 3.58 LASFSPNGHH398 pKa = 5.99 TSGQNTFTLVNKK410 pKa = 8.75 PANLAAITDD419 pKa = 3.51 ARR421 pKa = 11.84 FYY423 pKa = 11.53 SLDD426 pKa = 3.44 LKK428 pKa = 10.76 MKK430 pKa = 10.13 GVARR434 pKa = 11.84 NPLAPEE440 pKa = 4.08 SDD442 pKa = 3.73 SFDD445 pKa = 3.71 AATGPINLSWTPADD459 pKa = 3.6 MYY461 pKa = 10.98 GGKK464 pKa = 9.99 LDD466 pKa = 3.75 TTAITATEE474 pKa = 4.36 ANLSTHH480 pKa = 5.8 RR481 pKa = 11.84 QGGPFFTEE489 pKa = 4.21 SIVDD493 pKa = 3.22 ITGEE497 pKa = 4.02 INPTSAYY504 pKa = 10.54 AGILNQYY511 pKa = 8.75 YY512 pKa = 9.59 YY513 pKa = 11.47 SSVALKK519 pKa = 10.59 NN520 pKa = 3.48
Molecular weight: 56.98 kDa
Isoelectric point according different methods:
IPC2.protein.svr19 6.554
IPC2_protein 6.561
IPC_protein 6.781
Toseland 6.868
ProMoST 7.132
Dawson 7.263
Bjellqvist 7.117
Wikipedia 7.249
Rodwell 7.263
Grimsley 7.0
Solomon 7.322
Lehninger 7.337
Nozaki 7.439
DTASelect 7.541
Thurlkill 7.571
EMBOSS 7.629
Sillero 7.644
Patrickios 4.151
IPC_peptide 7.337
IPC2_peptide 7.073
IPC2.peptide.svr19 7.07
Protein with the highest isoelectric point:
>tr|W8PR73|W8PR73_9VIRU Capsid protein OS=Heterobasidion partitivirus 12 OX=1469905 PE=4 SV=1
MM1 pKa = 6.65 QTLLSAVYY9 pKa = 10.07 SIRR12 pKa = 11.84 DD13 pKa = 3.74 AIFGKK18 pKa = 8.31 TLSEE22 pKa = 3.74 PHH24 pKa = 5.94 GLNVNFHH31 pKa = 6.78 FDD33 pKa = 3.41 GYY35 pKa = 9.56 VTDD38 pKa = 4.91 EE39 pKa = 4.82 IKK41 pKa = 10.17 TSIPPRR47 pKa = 11.84 VEE49 pKa = 3.19 MWYY52 pKa = 10.69 SDD54 pKa = 3.59 YY55 pKa = 11.48 QKK57 pKa = 10.78 FLNPIIRR64 pKa = 11.84 ANFTGAEE71 pKa = 3.86 ADD73 pKa = 3.96 KK74 pKa = 10.79 IINGYY79 pKa = 7.67 HH80 pKa = 6.3 HH81 pKa = 7.62 PVATIPFMVEE91 pKa = 3.51 NLKK94 pKa = 10.99 KK95 pKa = 10.65 GDD97 pKa = 4.09 LSDD100 pKa = 4.26 HH101 pKa = 6.45 PVPHH105 pKa = 7.3 DD106 pKa = 3.44 EE107 pKa = 4.27 HH108 pKa = 7.69 YY109 pKa = 11.18 LRR111 pKa = 11.84 ARR113 pKa = 11.84 KK114 pKa = 8.14 MAADD118 pKa = 4.28 AFRR121 pKa = 11.84 PPRR124 pKa = 11.84 PIRR127 pKa = 11.84 PVHH130 pKa = 5.54 FADD133 pKa = 4.29 LRR135 pKa = 11.84 FYY137 pKa = 10.34 NWNWHH142 pKa = 6.18 PNVEE146 pKa = 4.03 EE147 pKa = 4.33 PYY149 pKa = 9.99 YY150 pKa = 10.35 SNKK153 pKa = 9.61 RR154 pKa = 11.84 AQEE157 pKa = 4.14 YY158 pKa = 9.3 VQAAHH163 pKa = 6.44 TLGLTPDD170 pKa = 3.18 ARR172 pKa = 11.84 MSFGNLRR179 pKa = 11.84 DD180 pKa = 3.71 YY181 pKa = 11.82 VFMDD185 pKa = 3.24 TRR187 pKa = 11.84 HH188 pKa = 5.91 YY189 pKa = 10.6 LHH191 pKa = 6.45 QIKK194 pKa = 10.29 RR195 pKa = 11.84 NEE197 pKa = 3.67 ISNPKK202 pKa = 8.3 TLWPLMKK209 pKa = 9.9 IHH211 pKa = 6.61 VKK213 pKa = 9.84 PALTGTDD220 pKa = 3.31 EE221 pKa = 3.97 QKK223 pKa = 10.35 IRR225 pKa = 11.84 VIYY228 pKa = 9.84 GVSKK232 pKa = 10.55 RR233 pKa = 11.84 HH234 pKa = 5.93 VLPQCMFFWPLFRR247 pKa = 11.84 HH248 pKa = 5.89 YY249 pKa = 10.4 IEE251 pKa = 5.74 NDD253 pKa = 3.07 TDD255 pKa = 3.84 PLLWGFEE262 pKa = 4.32 TILGGMMKK270 pKa = 10.3 LNSLMHH276 pKa = 6.73 RR277 pKa = 11.84 LYY279 pKa = 10.19 FQTFVTVDD287 pKa = 2.64 WSGFDD292 pKa = 3.55 LRR294 pKa = 11.84 SLFSIQRR301 pKa = 11.84 EE302 pKa = 4.03 IFDD305 pKa = 3.3 DD306 pKa = 2.94 WRR308 pKa = 11.84 TYY310 pKa = 10.99 FDD312 pKa = 4.36 FSNGYY317 pKa = 9.29 IPTVWYY323 pKa = 8.88 PNSTADD329 pKa = 4.25 PIQLEE334 pKa = 4.52 RR335 pKa = 11.84 LWNWQRR341 pKa = 11.84 DD342 pKa = 3.54 ACFNMPFVMPDD353 pKa = 2.68 KK354 pKa = 10.69 SVYY357 pKa = 9.76 RR358 pKa = 11.84 RR359 pKa = 11.84 LFRR362 pKa = 11.84 AIPSGLFVTQFLDD375 pKa = 3.21 SHH377 pKa = 6.7 YY378 pKa = 10.74 NYY380 pKa = 10.34 IMLLTILSAMGFDD393 pKa = 3.29 ITIEE397 pKa = 4.13 RR398 pKa = 11.84 IRR400 pKa = 11.84 ILVQGDD406 pKa = 3.91 DD407 pKa = 3.73 SLKK410 pKa = 10.81 NLIFFIPANQHH421 pKa = 6.58 DD422 pKa = 4.28 NFKK425 pKa = 11.45 AEE427 pKa = 4.29 FQRR430 pKa = 11.84 LATYY434 pKa = 10.81 YY435 pKa = 9.15 FDD437 pKa = 3.98 HH438 pKa = 6.68 VARR441 pKa = 11.84 PEE443 pKa = 3.64 KK444 pKa = 10.28 TEE446 pKa = 4.35 IYY448 pKa = 10.37 NSPQGVTVLGYY459 pKa = 9.26 TNNNGFPTRR468 pKa = 11.84 DD469 pKa = 3.91 PIKK472 pKa = 10.93 LMAQLYY478 pKa = 9.53 HH479 pKa = 6.54 PRR481 pKa = 11.84 QVGEE485 pKa = 3.82 RR486 pKa = 11.84 WKK488 pKa = 10.68 SVLMAKK494 pKa = 10.26 CAGFAYY500 pKa = 10.12 ASAYY504 pKa = 10.34 NYY506 pKa = 9.7 PKK508 pKa = 9.6 VTATLKK514 pKa = 8.79 TVYY517 pKa = 10.28 YY518 pKa = 10.16 KK519 pKa = 10.9 LAAKK523 pKa = 10.0 GFSPAALRR531 pKa = 11.84 TQRR534 pKa = 11.84 DD535 pKa = 3.05 IVLFGEE541 pKa = 4.92 AKK543 pKa = 9.82 FQVPTDD549 pKa = 3.74 HH550 pKa = 7.07 FPTAEE555 pKa = 3.8 EE556 pKa = 4.32 VQRR559 pKa = 11.84 HH560 pKa = 4.9 LRR562 pKa = 11.84 VPYY565 pKa = 10.16 KK566 pKa = 9.9 RR567 pKa = 11.84 TEE569 pKa = 4.09 EE570 pKa = 4.17 DD571 pKa = 3.45 SEE573 pKa = 5.6 SYY575 pKa = 10.98 FPMKK579 pKa = 10.46 HH580 pKa = 6.05 FLDD583 pKa = 4.86 FAA585 pKa = 6.06
Molecular weight: 68.71 kDa
Isoelectric point according different methods:
IPC2.protein.svr19 8.108
IPC2_protein 8.244
IPC_protein 8.185
Toseland 8.521
ProMoST 8.551
Dawson 8.916
Bjellqvist 8.814
Wikipedia 9.107
Rodwell 8.975
Grimsley 8.946
Solomon 9.004
Lehninger 8.946
Nozaki 8.741
DTASelect 8.756
Thurlkill 8.785
EMBOSS 9.019
Sillero 8.96
Patrickios 4.228
IPC_peptide 8.99
IPC2_peptide 7.6
IPC2.peptide.svr19 7.665
Peptides (in silico digests for buttom-up proteomics)
Below you can find
in silico digests of the whole proteome with Trypsin, Chymotrypsin, Trypsin+LysC, LysN, ArgC proteases suitable for different mass spec machines.
Try ESI
ChTry ESI
ArgC ESI
LysN ESI
TryLysC ESI
Try MALDI
ChTry MALDI
ArgC MALDI
LysN MALDI
TryLysC MALDI
Try LTQ
ChTry LTQ
ArgC LTQ
LysN LTQ
TryLysC LTQ
Try MSlow
ChTry MSlow
ArgC MSlow
LysN MSlow
TryLysC MSlow
Try MShigh
ChTry MShigh
ArgC MShigh
LysN MShigh
TryLysC MShigh
General Statistics
Number of major isoforms
Number of additional isoforms
Number of all proteins
Number of amino acids
Min. Seq. Length
Max. Seq. Length
Avg. Seq. Length
Avg. Mol. Weight
2
0
2
1105
520
585
552.5
62.85
Amino acid frequency
Ala
Cys
Asp
Glu
Phe
Gly
His
Ile
Lys
Leu
8.688 ± 1.65
0.633 ± 0.091
5.52 ± 0.348
3.529 ± 0.689
6.697 ± 0.363
4.887 ± 0.462
2.896 ± 0.779
5.339 ± 0.227
4.434 ± 0.393
8.688 ± 0.363
Met
Asn
Gln
Pro
Arg
Ser
Thr
Val
Trp
Tyr
3.258 ± 0.136
5.52 ± 0.552
6.878 ± 0.159
4.163 ± 0.431
5.158 ± 1.006
6.425 ± 1.362
5.792 ± 0.015
5.339 ± 0.227
1.629 ± 0.318
4.525 ± 0.84
Note: For amino acid frequency statistics the error has been estimated with the bootstraping (x100) at the protein level
Most of the basic statistics you can see at this page can be downloaded from this CSV file
For dipeptide frequency statistics click here