Pseudomonas phage Nerthus

Taxonomy: Viruses; Duplodnaviria; Heunggongvirae; Uroviricota; Caudoviricetes; Caudovirales; Autographiviridae; Colwellvirinae; Uliginvirus; Pseudomonas virus Nerthus

Average proteome isoelectric point is 6.32

Get precalculated fractions of proteins

Acidic
pI < 6.8
6.8-7.4
pI > 7.4
Basic
    
All



Virtual 2D-PAGE plot for 45 proteins (isoelectric point calculated using IPC2_protein)

Get csv file with sequences according to given criteria:
        -     Method 

     -  kDa    
                                                                                      

* You can choose from 21 different methods for calculating isoelectric point

Summary statistics related to proteome-wise predictions


    
Protein with the lowest isoelectric point:
>tr|A0A2S1GMN8|A0A2S1GMN8_9CAUD DNA-directed DNA polymerase OS=Pseudomonas phage Nerthus OX=2163984 PE=3 SV=1
MM1 pKa = 7.56ARR3 pKa = 11.84MTLTIEE9 pKa = 3.96VDD11 pKa = 3.58QLEE14 pKa = 5.01DD15 pKa = 5.23DD16 pKa = 4.37ADD18 pKa = 4.09CDD20 pKa = 3.74KK21 pKa = 11.0LANQMRR27 pKa = 11.84EE28 pKa = 4.25TLQEE32 pKa = 4.08AYY34 pKa = 9.63PYY36 pKa = 10.43IEE38 pKa = 4.35VNVLEE43 pKa = 4.12VDD45 pKa = 3.85GPEE48 pKa = 4.11LSLL51 pKa = 4.7

Molecular weight: 5.83 kDa
Isoelectric point according different methods:





Protein with the highest isoelectric point:
>tr|A0A2S1GMQ1|A0A2S1GMQ1_9CAUD Large terminase subunit OS=Pseudomonas phage Nerthus OX=2163984 PE=4 SV=1
MM1 pKa = 7.34SRR3 pKa = 11.84HH4 pKa = 6.39AYY6 pKa = 9.23FSNIGFQRR14 pKa = 11.84GLHH17 pKa = 6.07RR18 pKa = 11.84LPLDD22 pKa = 3.73LSKK25 pKa = 11.46VPVDD29 pKa = 3.36CRR31 pKa = 11.84FKK33 pKa = 11.01YY34 pKa = 9.68RR35 pKa = 11.84QAYY38 pKa = 8.73DD39 pKa = 3.19QAFALPNSTNPRR51 pKa = 11.84PPKK54 pKa = 10.62GNWFLRR60 pKa = 11.84LLIWLLRR67 pKa = 11.84FVGLLDD73 pKa = 3.85KK74 pKa = 11.34PEE76 pKa = 4.24ARR78 pKa = 11.84DD79 pKa = 3.47GASKK83 pKa = 10.59LRR85 pKa = 11.84AA86 pKa = 3.53

Molecular weight: 10.01 kDa
Isoelectric point according different methods:





Peptides (in silico digests for buttom-up proteomics)

Below you can find in silico digests of the whole proteome with Trypsin, Chymotrypsin, Trypsin+LysC, LysN, ArgC proteases suitable for different mass spec machines.

Try
ESI
ChTry
ESI
ArgC
ESI
LysN
ESI
TryLysC
ESI

Try
MALDI
ChTry
MALDI
ArgC
MALDI
LysN
MALDI
TryLysC
MALDI

Try
LTQ
ChTry
LTQ
ArgC
LTQ
LysN
LTQ
TryLysC
LTQ

Try
MSlow
ChTry
MSlow
ArgC
MSlow
LysN
MSlow
TryLysC
MSlow

Try
MShigh
ChTry
MShigh
ArgC
MShigh
LysN
MShigh
TryLysC
MShigh

General Statistics

Number of major isoforms

Number of additional isoforms

Number of all proteins

Number of amino acids

Min. Seq. Length

Max. Seq. Length

Avg. Seq. Length

Avg. Mol. Weight

45

0

45

13886

43

1257

308.6

34.37

Amino acid frequency

Ala

Cys

Asp

Glu

Phe

Gly

His

Ile

Lys

Leu

9.391 ± 0.461

0.958 ± 0.145

6.532 ± 0.286

6.417 ± 0.311

3.709 ± 0.251

7.749 ± 0.27

2.276 ± 0.226

4.688 ± 0.2

5.725 ± 0.251

7.994 ± 0.276

Met

Asn

Gln

Pro

Arg

Ser

Thr

Val

Trp

Tyr

3.032 ± 0.244

4.263 ± 0.172

4.119 ± 0.185

4.53 ± 0.345

5.66 ± 0.257

5.336 ± 0.272

6.136 ± 0.308

6.633 ± 0.28

1.491 ± 0.172

3.363 ± 0.244
Note: For amino acid frequency statistics the error has been estimated with the bootstraping (x100) at the protein level

Most of the basic statistics you can see at this page can be downloaded from this CSV file

For dipeptide frequency statistics click here
See this proteome in: uniprot_link
Proteome-pI is available under Creative Commons Attribution-NoDerivs license, for more details see here

Reference: Kozlowski LP. Proteome-pI 2.0: Proteome Isoelectric Point Database Update. Nucleic Acids Res. 2021, doi: 10.1093/nar/gkab944 Contact: Lukasz P. Kozlowski