Exiguobacterium phage vB_EalM-132

Taxonomy: Viruses; Duplodnaviria; Heunggongvirae; Uroviricota; Caudoviricetes; Caudovirales; Herelleviridae; Spounavirinae; unclassified Spounavirinae

Average proteome isoelectric point is 6.12

Get precalculated fractions of proteins

Acidic
pI < 6.8
6.8-7.4
pI > 7.4
Basic
    
All



Virtual 2D-PAGE plot for 168 proteins (isoelectric point calculated using IPC2_protein)

Get csv file with sequences according to given criteria:
        -     Method 

     -  kDa    
                                                                                      

* You can choose from 21 different methods for calculating isoelectric point

Summary statistics related to proteome-wise predictions


    
Protein with the lowest isoelectric point:
>tr|A0A3G3BW77|A0A3G3BW77_9CAUD Uncharacterized protein OS=Exiguobacterium phage vB_EalM-132 OX=2419623 GN=EalM132_00025 PE=4 SV=1
MM1 pKa = 8.0KK2 pKa = 10.62YY3 pKa = 10.2PLNQCSDD10 pKa = 3.56CTHH13 pKa = 7.38RR14 pKa = 11.84NGCDD18 pKa = 3.34LLSKK22 pKa = 10.34IRR24 pKa = 11.84FWANRR29 pKa = 11.84NDD31 pKa = 4.3FIDD34 pKa = 4.5MSDD37 pKa = 3.7ATCKK41 pKa = 10.44SYY43 pKa = 9.1QTDD46 pKa = 4.35YY47 pKa = 10.21YY48 pKa = 10.61TPEE51 pKa = 4.16DD52 pKa = 4.21LGWKK56 pKa = 9.89NLMEE60 pKa = 4.22PTPNKK65 pKa = 10.49AYY67 pKa = 10.37DD68 pKa = 3.21ISYY71 pKa = 11.09EE72 pKa = 3.93EE73 pKa = 4.36LSEE76 pKa = 4.26NEE78 pKa = 3.9YY79 pKa = 11.0FLALEE84 pKa = 4.77DD85 pKa = 4.44ALMDD89 pKa = 4.53CGLAPDD95 pKa = 4.4YY96 pKa = 10.85IIVSTEE102 pKa = 3.59FVTNLIPDD110 pKa = 4.31RR111 pKa = 11.84FSGTNLRR118 pKa = 11.84LLEE121 pKa = 4.12LFGIPIEE128 pKa = 4.46INDD131 pKa = 3.93TLGTRR136 pKa = 11.84FIIQGEE142 pKa = 4.11DD143 pKa = 2.98MKK145 pKa = 10.51TGEE148 pKa = 4.6MVALAYY154 pKa = 9.47DD155 pKa = 3.66ALEE158 pKa = 4.65DD159 pKa = 4.88EE160 pKa = 5.03EE161 pKa = 6.61DD162 pKa = 3.77GDD164 pKa = 4.3

Molecular weight: 18.79 kDa
Isoelectric point according different methods:





Protein with the highest isoelectric point:
>tr|A0A3G3BWU5|A0A3G3BWU5_9CAUD Uncharacterized protein OS=Exiguobacterium phage vB_EalM-132 OX=2419623 GN=EalM132_00125 PE=4 SV=1
MM1 pKa = 6.84RR2 pKa = 11.84QEE4 pKa = 3.82RR5 pKa = 11.84KK6 pKa = 8.9EE7 pKa = 3.91EE8 pKa = 3.57YY9 pKa = 9.98RR10 pKa = 11.84NRR12 pKa = 11.84VNYY15 pKa = 9.8KK16 pKa = 9.15HH17 pKa = 6.82ANFVLRR23 pKa = 11.84FHH25 pKa = 7.12VRR27 pKa = 11.84QCRR30 pKa = 11.84LL31 pKa = 2.97

Molecular weight: 4.08 kDa
Isoelectric point according different methods:





Peptides (in silico digests for buttom-up proteomics)

Below you can find in silico digests of the whole proteome with Trypsin, Chymotrypsin, Trypsin+LysC, LysN, ArgC proteases suitable for different mass spec machines.

Try
ESI
ChTry
ESI
ArgC
ESI
LysN
ESI
TryLysC
ESI

Try
MALDI
ChTry
MALDI
ArgC
MALDI
LysN
MALDI
TryLysC
MALDI

Try
LTQ
ChTry
LTQ
ArgC
LTQ
LysN
LTQ
TryLysC
LTQ

Try
MSlow
ChTry
MSlow
ArgC
MSlow
LysN
MSlow
TryLysC
MSlow

Try
MShigh
ChTry
MShigh
ArgC
MShigh
LysN
MShigh
TryLysC
MShigh

General Statistics

Number of major isoforms

Number of additional isoforms

Number of all proteins

Number of amino acids

Min. Seq. Length

Max. Seq. Length

Avg. Seq. Length

Avg. Mol. Weight

168

0

168

38565

31

1126

229.6

25.93

Amino acid frequency

Ala

Cys

Asp

Glu

Phe

Gly

His

Ile

Lys

Leu

6.426 ± 0.186

0.69 ± 0.068

6.48 ± 0.18

7.452 ± 0.277

3.218 ± 0.112

6.285 ± 0.226

2.505 ± 0.169

6.371 ± 0.156

7.242 ± 0.295

8.295 ± 0.188

Met

Asn

Gln

Pro

Arg

Ser

Thr

Val

Trp

Tyr

2.756 ± 0.105

5.121 ± 0.147

3.542 ± 0.125

3.392 ± 0.13

4.452 ± 0.118

6.49 ± 0.198

6.737 ± 0.343

7.04 ± 0.199

1.177 ± 0.08

4.328 ± 0.134
Note: For amino acid frequency statistics the error has been estimated with the bootstraping (x100) at the protein level

Most of the basic statistics you can see at this page can be downloaded from this CSV file

For dipeptide frequency statistics click here
See this proteome in: uniprot_link
Proteome-pI is available under Creative Commons Attribution-NoDerivs license, for more details see here

Reference: Kozlowski LP. Proteome-pI 2.0: Proteome Isoelectric Point Database Update. Nucleic Acids Res. 2021, doi: 10.1093/nar/gkab944 Contact: Lukasz P. Kozlowski