Glaciihabitans arcticus
Average proteome isoelectric point is 5.89
Get precalculated fractions of proteins
Acidic
pI < 6.8
6.8-7.4
pI > 7.4
Basic
All
Note: above files contain also dissociation constants (pKa)
Virtual 2D-PAGE plot for 2984 proteins (isoelectric point calculated using IPC2_protein)
Get csv file with sequences according to given criteria:
* You can choose from 21 different methods for calculating isoelectric point
Summary statistics related to proteome-wise predictions
Protein with the lowest isoelectric point:
>tr|A0A4Q9GXY9|A0A4Q9GXY9_9MICO ABC transporter substrate-binding protein OS=Glaciihabitans arcticus OX=2668039 GN=EYE40_12340 PE=3 SV=1
MM1 pKa = 7.81 AEE3 pKa = 4.39 GDD5 pKa = 4.35 NPFPWGLTPGGKK17 pKa = 9.27 PEE19 pKa = 5.1 DD20 pKa = 4.46 EE21 pKa = 4.55 EE22 pKa = 4.76 TPPTPPVAPEE32 pKa = 5.18 PIDD35 pKa = 3.18 QGYY38 pKa = 8.09 FAAVTIEE45 pKa = 4.58 EE46 pKa = 4.51 PTSPAPEE53 pKa = 4.31 GLPTEE58 pKa = 4.88 AMASQEE64 pKa = 3.75 WDD66 pKa = 3.68 GPATEE71 pKa = 5.35 AMPAQEE77 pKa = 4.26 WEE79 pKa = 4.7 GPPTDD84 pKa = 4.45 PFPYY88 pKa = 9.5 HH89 pKa = 6.59 QPAPWEE95 pKa = 4.15 PPPVDD100 pKa = 3.49 EE101 pKa = 5.38 SLGGTSNALAAQPIGLDD118 pKa = 3.39 APVGEE123 pKa = 4.76 SVPGEE128 pKa = 3.95 NTDD131 pKa = 4.51 AIEE134 pKa = 4.33 DD135 pKa = 3.92 LFGEE139 pKa = 5.04 GSFQEE144 pKa = 4.36 YY145 pKa = 9.77 EE146 pKa = 3.91 DD147 pKa = 5.22 AIVVLEE153 pKa = 4.73 GIPFPGKK160 pKa = 10.2 AVEE163 pKa = 4.06 PAAAPGALVAPPPTLLPAEE182 pKa = 4.27 PMPRR186 pKa = 11.84 LQRR189 pKa = 11.84 NLLWAAGGLVAALALVAVFLGGVRR213 pKa = 11.84 LAPQFAAAPEE223 pKa = 4.37 APAVPSSAPTLAPEE237 pKa = 4.43 SLGPLPAGVHH247 pKa = 5.46 AWSDD251 pKa = 3.68 LLGTEE256 pKa = 4.2 CVEE259 pKa = 4.73 PYY261 pKa = 10.39 VSPWDD266 pKa = 3.61 KK267 pKa = 10.93 EE268 pKa = 4.23 FTVVDD273 pKa = 3.63 CSGPHH278 pKa = 5.36 TAQLVATGVFEE289 pKa = 6.2 DD290 pKa = 5.07 SILDD294 pKa = 4.0 AYY296 pKa = 9.86 PGLEE300 pKa = 3.94 EE301 pKa = 4.08 LQSRR305 pKa = 11.84 MGQLCSNATNVDD317 pKa = 3.28 YY318 pKa = 9.2 TAAKK322 pKa = 9.72 AYY324 pKa = 11.0 SDD326 pKa = 3.57 IQVSASYY333 pKa = 10.62 AADD336 pKa = 3.57 EE337 pKa = 4.26 QSWIDD342 pKa = 3.09 GDD344 pKa = 4.0 RR345 pKa = 11.84 NYY347 pKa = 10.82 FCFVSRR353 pKa = 11.84 STGEE357 pKa = 3.98 PLTSSVAMPDD367 pKa = 2.93 RR368 pKa = 11.84 PTPVVPVVPEE378 pKa = 4.0 PEE380 pKa = 3.86 PP381 pKa = 3.96
Molecular weight: 39.82 kDa
Isoelectric point according different methods:
IPC2.protein.svr19 3.728
IPC2_protein 3.706
IPC_protein 3.694
Toseland 3.503
ProMoST 3.821
Dawson 3.656
Bjellqvist 3.808
Wikipedia 3.541
Rodwell 3.516
Grimsley 3.414
Solomon 3.643
Lehninger 3.605
Nozaki 3.77
DTASelect 3.923
Thurlkill 3.528
EMBOSS 3.554
Sillero 3.808
Patrickios 1.151
IPC_peptide 3.643
IPC2_peptide 3.783
IPC2.peptide.svr19 3.72
Protein with the highest isoelectric point:
>tr|A0A4Q9GUL4|A0A4Q9GUL4_9MICO ADP-dependent (S)-NAD(P)H-hydrate dehydratase OS=Glaciihabitans arcticus OX=2668039 GN=nnrD PE=3 SV=1
MM1 pKa = 7.4 GSVIKK6 pKa = 10.42 KK7 pKa = 8.47 RR8 pKa = 11.84 RR9 pKa = 11.84 KK10 pKa = 9.22 RR11 pKa = 11.84 MAKK14 pKa = 9.41 KK15 pKa = 9.87 KK16 pKa = 9.7 HH17 pKa = 5.84 RR18 pKa = 11.84 KK19 pKa = 8.56 LLRR22 pKa = 11.84 KK23 pKa = 7.78 TRR25 pKa = 11.84 HH26 pKa = 3.65 QRR28 pKa = 11.84 RR29 pKa = 11.84 NKK31 pKa = 9.78 KK32 pKa = 9.85
Molecular weight: 4.08 kDa
Isoelectric point according different methods:
IPC2.protein.svr19 9.493
IPC2_protein 11.14
IPC_protein 12.691
Toseland 12.866
ProMoST 13.349
Dawson 12.866
Bjellqvist 12.852
Wikipedia 13.334
Rodwell 12.735
Grimsley 12.91
Solomon 13.349
Lehninger 13.261
Nozaki 12.866
DTASelect 12.852
Thurlkill 12.866
EMBOSS 13.364
Sillero 12.866
Patrickios 12.457
IPC_peptide 13.349
IPC2_peptide 12.34
IPC2.peptide.svr19 9.071
Peptides (in silico digests for buttom-up proteomics)
Below you can find
in silico digests of the whole proteome with Trypsin, Chymotrypsin, Trypsin+LysC, LysN, ArgC proteases suitable for different mass spec machines.
Try ESI
ChTry ESI
ArgC ESI
LysN ESI
TryLysC ESI
Try MALDI
ChTry MALDI
ArgC MALDI
LysN MALDI
TryLysC MALDI
Try LTQ
ChTry LTQ
ArgC LTQ
LysN LTQ
TryLysC LTQ
Try MSlow
ChTry MSlow
ArgC MSlow
LysN MSlow
TryLysC MSlow
Try MShigh
ChTry MShigh
ArgC MShigh
LysN MShigh
TryLysC MShigh
General Statistics
Number of major isoforms
Number of additional isoforms
Number of all proteins
Number of amino acids
Min. Seq. Length
Max. Seq. Length
Avg. Seq. Length
Avg. Mol. Weight
2984
0
2984
981908
24
6078
329.1
35.16
Amino acid frequency
Ala
Cys
Asp
Glu
Phe
Gly
His
Ile
Lys
Leu
12.682 ± 0.054
0.447 ± 0.011
5.941 ± 0.041
5.608 ± 0.057
3.377 ± 0.03
8.856 ± 0.053
1.748 ± 0.024
4.98 ± 0.036
2.422 ± 0.037
10.316 ± 0.07
Met
Asn
Gln
Pro
Arg
Ser
Thr
Val
Trp
Tyr
1.652 ± 0.02
2.393 ± 0.037
5.191 ± 0.035
2.71 ± 0.023
6.311 ± 0.062
6.315 ± 0.041
6.578 ± 0.089
8.909 ± 0.041
1.465 ± 0.018
2.097 ± 0.024
Note: For amino acid frequency statistics the error has been estimated with the bootstraping (x100) at the protein level
Most of the basic statistics you can see at this page can be downloaded from this CSV file
For dipeptide frequency statistics click here