Streptococcus satellite phage Javan735
Average proteome isoelectric point is 7.26
Get precalculated fractions of proteins
Acidic
pI < 6.8
6.8-7.4
pI > 7.4
Basic
All
Note: above files contain also dissociation constants (pKa)
Virtual 2D-PAGE plot for 25 proteins (isoelectric point calculated using IPC2_protein)
Get csv file with sequences according to given criteria:
* You can choose from 21 different methods for calculating isoelectric point
Summary statistics related to proteome-wise predictions
Protein with the lowest isoelectric point:
>tr|A0A4D5ZUK5|A0A4D5ZUK5_9VIRU Helicase loader OS=Streptococcus satellite phage Javan735 OX=2558829 GN=JavanS735_0016 PE=4 SV=1
MM1 pKa = 7.27 NRR3 pKa = 11.84 LKK5 pKa = 10.53 EE6 pKa = 3.82 LRR8 pKa = 11.84 KK9 pKa = 9.38 RR10 pKa = 11.84 DD11 pKa = 4.0 KK12 pKa = 9.42 ITQVAFAKK20 pKa = 10.85 DD21 pKa = 3.21 NGIPLRR27 pKa = 11.84 TLQSWEE33 pKa = 3.82 NGEE36 pKa = 4.12 NQIKK40 pKa = 9.89 PEE42 pKa = 4.02 KK43 pKa = 9.73 AQQLADD49 pKa = 3.26 IFGVSVGYY57 pKa = 10.64 LLGFNIDD64 pKa = 5.0 DD65 pKa = 3.86 VTEE68 pKa = 4.31 DD69 pKa = 4.36 EE70 pKa = 5.3 INFHH74 pKa = 6.12 NNVMEE79 pKa = 4.32 RR80 pKa = 11.84 MNKK83 pKa = 8.7 EE84 pKa = 3.4 AFIRR88 pKa = 11.84 FLDD91 pKa = 5.22 FITLSDD97 pKa = 3.39 IVLSDD102 pKa = 3.59 KK103 pKa = 10.55 QIEE106 pKa = 4.43 MIFYY110 pKa = 9.99 QLQDD114 pKa = 3.63 LSEE117 pKa = 4.55 LNSDD121 pKa = 3.51 YY122 pKa = 10.75 RR123 pKa = 11.84 YY124 pKa = 8.92 TEE126 pKa = 3.76 TDD128 pKa = 3.32 VEE130 pKa = 4.54 KK131 pKa = 10.49 LKK133 pKa = 11.32 SMYY136 pKa = 10.06 SVKK139 pKa = 10.33 LNYY142 pKa = 9.52 MPTEE146 pKa = 4.01 GLIKK150 pKa = 10.2 ISNILYY156 pKa = 10.27 KK157 pKa = 10.35 EE158 pKa = 4.06 DD159 pKa = 4.34 AIEE162 pKa = 4.05 EE163 pKa = 4.03 QFEE166 pKa = 4.35 QYY168 pKa = 10.9 KK169 pKa = 10.63 KK170 pKa = 10.74 IIDD173 pKa = 3.5
Molecular weight: 20.42 kDa
Isoelectric point according different methods:
IPC2.protein.svr19 4.791
IPC2_protein 4.762
IPC_protein 4.66
Toseland 4.507
ProMoST 4.698
Dawson 4.596
Bjellqvist 4.774
Wikipedia 4.469
Rodwell 4.495
Grimsley 4.418
Solomon 4.596
Lehninger 4.546
Nozaki 4.711
DTASelect 4.863
Thurlkill 4.507
EMBOSS 4.482
Sillero 4.774
Patrickios 4.151
IPC_peptide 4.609
IPC2_peptide 4.762
IPC2.peptide.svr19 4.757
Protein with the highest isoelectric point:
>tr|A0A4D5ZUJ7|A0A4D5ZUJ7_9VIRU HTH cro/C1-type domain-containing protein OS=Streptococcus satellite phage Javan735 OX=2558829 GN=JavanS735_0006 PE=4 SV=1
MM1 pKa = 7.79 RR2 pKa = 11.84 NGVWVVYY9 pKa = 8.83 SFKK12 pKa = 10.96 YY13 pKa = 10.71 GSLTHH18 pKa = 6.73 LLEE21 pKa = 4.86 FGHH24 pKa = 6.93 IKK26 pKa = 10.57 RR27 pKa = 11.84 NGGRR31 pKa = 11.84 TKK33 pKa = 10.28 AYY35 pKa = 8.33 PHH37 pKa = 6.61 LRR39 pKa = 11.84 PAEE42 pKa = 3.9 LNAIQKK48 pKa = 7.51 FTEE51 pKa = 4.66 RR52 pKa = 11.84 IKK54 pKa = 11.09 NISKK58 pKa = 10.68
Molecular weight: 6.77 kDa
Isoelectric point according different methods:
IPC2.protein.svr19 9.283
IPC2_protein 9.984
IPC_protein 10.54
Toseland 10.73
ProMoST 10.423
Dawson 10.847
Bjellqvist 10.496
Wikipedia 11.008
Rodwell 11.286
Grimsley 10.891
Solomon 10.906
Lehninger 10.891
Nozaki 10.701
DTASelect 10.496
Thurlkill 10.73
EMBOSS 11.125
Sillero 10.76
Patrickios 11.052
IPC_peptide 10.921
IPC2_peptide 9.18
IPC2.peptide.svr19 8.473
Peptides (in silico digests for buttom-up proteomics)
Below you can find
in silico digests of the whole proteome with Trypsin, Chymotrypsin, Trypsin+LysC, LysN, ArgC proteases suitable for different mass spec machines.
Try ESI
ChTry ESI
ArgC ESI
LysN ESI
TryLysC ESI
Try MALDI
ChTry MALDI
ArgC MALDI
LysN MALDI
TryLysC MALDI
Try LTQ
ChTry LTQ
ArgC LTQ
LysN LTQ
TryLysC LTQ
Try MSlow
ChTry MSlow
ArgC MSlow
LysN MSlow
TryLysC MSlow
Try MShigh
ChTry MShigh
ArgC MShigh
LysN MShigh
TryLysC MShigh
General Statistics
Number of major isoforms
Number of additional isoforms
Number of all proteins
Number of amino acids
Min. Seq. Length
Max. Seq. Length
Avg. Seq. Length
Avg. Mol. Weight
25
0
25
3662
51
388
146.5
16.94
Amino acid frequency
Ala
Cys
Asp
Glu
Phe
Gly
His
Ile
Lys
Leu
5.134 ± 0.456
0.628 ± 0.142
5.898 ± 0.431
7.865 ± 0.639
4.233 ± 0.354
4.615 ± 0.468
1.775 ± 0.287
7.155 ± 0.424
10.24 ± 0.722
10.404 ± 0.517
Met
Asn
Gln
Pro
Arg
Ser
Thr
Val
Trp
Tyr
1.993 ± 0.24
6.09 ± 0.468
2.458 ± 0.218
3.741 ± 0.409
4.806 ± 0.353
6.636 ± 0.545
6.281 ± 0.41
4.67 ± 0.374
0.819 ± 0.147
4.56 ± 0.336
Note: For amino acid frequency statistics the error has been estimated with the bootstraping (x100) at the protein level
Most of the basic statistics you can see at this page can be downloaded from this CSV file
For dipeptide frequency statistics click here