Amino acid dipepetide frequency for Dictyostelium discoideum (Slime mold)

Apart from single amino acid frequencies one can also calculate so called amino acid dipeptide frequency.

There is 400 possibilites (441 if we consider Xaa as additional 21st amino acid representing all non-standard or unknown amino acids). Thus, if the dipeptides would be present randomly in proteins each amino acid dipeptide should be present with 0.25% frequency. As it is not the case in the nature, for better visablity all more than expected dipepetides are marked by red, and those which are underrepresented are marked by blue in the table.

All values are presented as per milles (‰), therefore need to be multiplied by 10^-3.

For more information see sequence space article on Wikipedia.

	Ala	Cys	Asp	Glu	Phe	Gly	His	Ile	Lys	Leu	Met	Asn	Pro	Gln	Arg	Ser	Thr	Val	Trp	Tyr	Xaa
Ala	1.795AlaAla: 1.795 ± 0.028	0.51AlaCys: 0.51 ± 0.013	1.365AlaAsp: 1.365 ± 0.016	1.422AlaGlu: 1.422 ± 0.023	1.449AlaPhe: 1.449 ± 0.016	1.525AlaGly: 1.525 ± 0.028	0.548AlaHis: 0.548 ± 0.011	2.779AlaIle: 2.779 ± 0.025	2.045AlaLys: 2.045 ± 0.023	2.834AlaLeu: 2.834 ± 0.027	0.625AlaMet: 0.625 ± 0.012	2.033AlaAsn: 2.033 ± 0.02	1.321AlaPro: 1.321 ± 0.021	1.219AlaGln: 1.219 ± 0.017	0.963AlaArg: 0.963 ± 0.016	2.842AlaSer: 2.842 ± 0.029	2.315AlaThr: 2.315 ± 0.028	1.518AlaVal: 1.518 ± 0.021	0.249AlaTrp: 0.249 ± 0.007	0.951AlaTyr: 0.951 ± 0.015	0.0AlaXaa: 0.0 ± 0.0
Cys	0.446CysAla: 0.446 ± 0.01	0.324CysCys: 0.324 ± 0.009	0.789CysAsp: 0.789 ± 0.017	0.691CysGlu: 0.691 ± 0.012	0.782CysPhe: 0.782 ± 0.014	0.864CysGly: 0.864 ± 0.015	0.258CysHis: 0.258 ± 0.008	1.1CysIle: 1.1 ± 0.016	1.191CysLys: 1.191 ± 0.015	1.327CysLeu: 1.327 ± 0.016	0.205CysMet: 0.205 ± 0.005	1.168CysAsn: 1.168 ± 0.018	0.599CysPro: 0.599 ± 0.013	0.528CysGln: 0.528 ± 0.011	0.417CysArg: 0.417 ± 0.008	1.422CysSer: 1.422 ± 0.025	0.801CysThr: 0.801 ± 0.023	0.656CysVal: 0.656 ± 0.012	0.141CysTrp: 0.141 ± 0.004	0.525CysTyr: 0.525 ± 0.009	0.0CysXaa: 0.0 ± 0.0
Asp	1.395AspAla: 1.395 ± 0.018	0.882AspCys: 0.882 ± 0.016	4.666AspAsp: 4.666 ± 0.052	3.944AspGlu: 3.944 ± 0.038	2.68AspPhe: 2.68 ± 0.022	2.566AspGly: 2.566 ± 0.03	0.961AspHis: 0.961 ± 0.012	4.387AspIle: 4.387 ± 0.03	3.611AspLys: 3.611 ± 0.031	4.421AspLeu: 4.421 ± 0.032	0.753AspMet: 0.753 ± 0.011	4.633AspAsn: 4.633 ± 0.032	1.78AspPro: 1.78 ± 0.017	2.228AspGln: 2.228 ± 0.022	1.605AspArg: 1.605 ± 0.03	4.65AspSer: 4.65 ± 0.039	2.204AspThr: 2.204 ± 0.018	1.986AspVal: 1.986 ± 0.022	0.451AspTrp: 0.451 ± 0.009	2.207AspTyr: 2.207 ± 0.021	0.0AspXaa: 0.0 ± 0.0
Glu	1.768GluAla: 1.768 ± 0.022	0.806GluCys: 0.806 ± 0.014	3.223GluAsp: 3.223 ± 0.034	5.544GluGlu: 5.544 ± 0.097	2.858GluPhe: 2.858 ± 0.024	1.86GluGly: 1.86 ± 0.019	0.769GluHis: 0.769 ± 0.013	5.598GluIle: 5.598 ± 0.034	6.006GluLys: 6.006 ± 0.076	4.97GluLeu: 4.97 ± 0.037	1.112GluMet: 1.112 ± 0.012	5.432GluAsn: 5.432 ± 0.042	1.51GluPro: 1.51 ± 0.018	2.55GluGln: 2.55 ± 0.027	2.122GluArg: 2.122 ± 0.034	4.471GluSer: 4.471 ± 0.034	2.866GluThr: 2.866 ± 0.023	2.164GluVal: 2.164 ± 0.026	0.501GluTrp: 0.501 ± 0.01	2.134GluTyr: 2.134 ± 0.02	0.0GluXaa: 0.0 ± 0.0
Phe	1.406PheAla: 1.406 ± 0.016	0.663PheCys: 0.663 ± 0.01	2.674PheAsp: 2.674 ± 0.023	2.826PheGlu: 2.826 ± 0.023	2.502PhePhe: 2.502 ± 0.03	2.495PheGly: 2.495 ± 0.027	0.811PheHis: 0.811 ± 0.013	4.267PheIle: 4.267 ± 0.03	4.346PheLys: 4.346 ± 0.037	4.071PheLeu: 4.071 ± 0.03	0.705PheMet: 0.705 ± 0.011	4.41PheAsn: 4.41 ± 0.036	1.483PhePro: 1.483 ± 0.016	1.893PheGln: 1.893 ± 0.019	1.215PheArg: 1.215 ± 0.014	4.414PheSer: 4.414 ± 0.033	2.526PheThr: 2.526 ± 0.027	2.016PheVal: 2.016 ± 0.021	0.361PheTrp: 0.361 ± 0.008	1.979PheTyr: 1.979 ± 0.02	0.0PheXaa: 0.0 ± 0.0
Gly	1.737GlyAla: 1.737 ± 0.025	0.808GlyCys: 0.808 ± 0.016	2.551GlyAsp: 2.551 ± 0.028	2.345GlyGlu: 2.345 ± 0.023	2.292GlyPhe: 2.292 ± 0.022	4.285GlyGly: 4.285 ± 0.051	0.848GlyHis: 0.848 ± 0.013	3.538GlyIle: 3.538 ± 0.029	2.93GlyLys: 2.93 ± 0.029	3.443GlyLeu: 3.443 ± 0.03	0.701GlyMet: 0.701 ± 0.013	3.496GlyAsn: 3.496 ± 0.037	1.042GlyPro: 1.042 ± 0.017	1.303GlyGln: 1.303 ± 0.015	1.372GlyArg: 1.372 ± 0.017	4.717GlySer: 4.717 ± 0.045	2.326GlyThr: 2.326 ± 0.026	2.478GlyVal: 2.478 ± 0.029	0.437GlyTrp: 0.437 ± 0.009	1.8GlyTyr: 1.8 ± 0.02	0.0GlyXaa: 0.0 ± 0.0
His	0.462HisAla: 0.462 ± 0.009	0.332HisCys: 0.332 ± 0.008	0.73HisAsp: 0.73 ± 0.012	0.698HisGlu: 0.698 ± 0.01	0.973HisPhe: 0.973 ± 0.012	0.762HisGly: 0.762 ± 0.013	1.026HisHis: 1.026 ± 0.033	1.308HisIle: 1.308 ± 0.016	1.047HisLys: 1.047 ± 0.014	1.802HisLeu: 1.802 ± 0.018	0.271HisMet: 0.271 ± 0.006	1.288HisAsn: 1.288 ± 0.019	0.812HisPro: 0.812 ± 0.013	1.304HisGln: 1.304 ± 0.026	0.647HisArg: 0.647 ± 0.012	1.682HisSer: 1.682 ± 0.019	0.842HisThr: 0.842 ± 0.014	0.653HisVal: 0.653 ± 0.009	0.17HisTrp: 0.17 ± 0.005	0.788HisTyr: 0.788 ± 0.012	0.0HisXaa: 0.0 ± 0.0
Ile	2.603IleAla: 2.603 ± 0.024	1.18IleCys: 1.18 ± 0.015	5.023IleAsp: 5.023 ± 0.028	5.658IleGlu: 5.658 ± 0.034	3.777IlePhe: 3.777 ± 0.029	3.9IleGly: 3.9 ± 0.033	1.474IleHis: 1.474 ± 0.015	7.566IleIle: 7.566 ± 0.056	7.437IleLys: 7.437 ± 0.044	7.249IleLeu: 7.249 ± 0.05	1.136IleMet: 1.136 ± 0.013	8.265IleAsn: 8.265 ± 0.06	3.674IlePro: 3.674 ± 0.024	3.616IleGln: 3.616 ± 0.028	2.272IleArg: 2.272 ± 0.02	7.463IleSer: 7.463 ± 0.043	4.681IleThr: 4.681 ± 0.033	3.849IleVal: 3.849 ± 0.031	0.599IleTrp: 0.599 ± 0.01	2.948IleTyr: 2.948 ± 0.027	0.0IleXaa: 0.0 ± 0.0
Lys	2.15LysAla: 2.15 ± 0.023	1.033LysCys: 1.033 ± 0.015	4.228LysAsp: 4.228 ± 0.035	6.323LysGlu: 6.323 ± 0.074	3.314LysPhe: 3.314 ± 0.027	2.594LysGly: 2.594 ± 0.027	1.227LysHis: 1.227 ± 0.014	6.251LysIle: 6.251 ± 0.039	7.565LysLys: 7.565 ± 0.063	7.024LysLeu: 7.024 ± 0.046	1.414LysMet: 1.414 ± 0.015	6.327LysAsn: 6.327 ± 0.036	2.762LysPro: 2.762 ± 0.024	3.693LysGln: 3.693 ± 0.031	2.797LysArg: 2.797 ± 0.024	6.908LysSer: 6.908 ± 0.045	4.0LysThr: 4.0 ± 0.029	3.509LysVal: 3.509 ± 0.026	0.575LysTrp: 0.575 ± 0.01	2.928LysTyr: 2.928 ± 0.028	0.0LysXaa: 0.0 ± 0.0
Leu	2.946LeuAla: 2.946 ± 0.033	1.087LeuCys: 1.087 ± 0.013	4.274LeuAsp: 4.274 ± 0.027	5.24LeuGlu: 5.24 ± 0.036	4.74LeuPhe: 4.74 ± 0.04	3.271LeuGly: 3.271 ± 0.027	1.377LeuHis: 1.377 ± 0.017	7.572LeuIle: 7.572 ± 0.053	7.756LeuLys: 7.756 ± 0.047	8.691LeuLeu: 8.691 ± 0.066	1.479LeuMet: 1.479 ± 0.019	7.28LeuAsn: 7.28 ± 0.046	3.75LeuPro: 3.75 ± 0.031	3.487LeuGln: 3.487 ± 0.033	2.351LeuArg: 2.351 ± 0.023	8.193LeuSer: 8.193 ± 0.045	4.478LeuThr: 4.478 ± 0.032	4.177LeuVal: 4.177 ± 0.029	0.58LeuTrp: 0.58 ± 0.01	2.986LeuTyr: 2.986 ± 0.025	0.0LeuXaa: 0.0 ± 0.0
Met	0.77MetAla: 0.77 ± 0.013	0.186MetCys: 0.186 ± 0.005	1.045MetAsp: 1.045 ± 0.013	1.117MetGlu: 1.117 ± 0.016	0.717MetPhe: 0.717 ± 0.012	0.894MetGly: 0.894 ± 0.014	0.182MetHis: 0.182 ± 0.005	1.438MetIle: 1.438 ± 0.015	1.374MetLys: 1.374 ± 0.014	1.149MetLeu: 1.149 ± 0.014	0.398MetMet: 0.398 ± 0.01	1.336MetAsn: 1.336 ± 0.02	0.426MetPro: 0.426 ± 0.009	0.423MetGln: 0.423 ± 0.009	0.397MetArg: 0.397 ± 0.008	1.298MetSer: 1.298 ± 0.014	0.829MetThr: 0.829 ± 0.012	1.072MetVal: 1.072 ± 0.015	0.108MetTrp: 0.108 ± 0.004	0.474MetTyr: 0.474 ± 0.009	0.0MetXaa: 0.0 ± 0.0
Asn	1.91AsnAla: 1.91 ± 0.021	1.224AsnCys: 1.224 ± 0.019	5.055AsnAsp: 5.055 ± 0.037	5.351AsnGlu: 5.351 ± 0.044	3.582AsnPhe: 3.582 ± 0.032	4.404AsnGly: 4.404 ± 0.042	1.746AsnHis: 1.746 ± 0.021	7.498AsnIle: 7.498 ± 0.052	6.727AsnLys: 6.727 ± 0.046	6.61AsnLeu: 6.61 ± 0.045	1.062AsnMet: 1.062 ± 0.017	37.449AsnAsn: 37.449 ± 0.573	2.589AsnPro: 2.589 ± 0.025	4.702AsnGln: 4.702 ± 0.045	2.164AsnArg: 2.164 ± 0.019	9.91AsnSer: 9.91 ± 0.077	4.517AsnThr: 4.517 ± 0.035	3.07AsnVal: 3.07 ± 0.022	0.541AsnTrp: 0.541 ± 0.01	3.491AsnTyr: 3.491 ± 0.031	0.0AsnXaa: 0.0 ± 0.0
Pro	1.032ProAla: 1.032 ± 0.016	0.385ProCys: 0.385 ± 0.011	1.421ProAsp: 1.421 ± 0.018	1.642ProGlu: 1.642 ± 0.02	1.852ProPhe: 1.852 ± 0.021	1.18ProGly: 1.18 ± 0.025	0.701ProHis: 0.701 ± 0.014	3.997ProIle: 3.997 ± 0.027	2.654ProLys: 2.654 ± 0.025	3.494ProLeu: 3.494 ± 0.029	0.668ProMet: 0.668 ± 0.011	3.173ProAsn: 3.173 ± 0.028	3.112ProPro: 3.112 ± 0.053	2.202ProGln: 2.202 ± 0.033	0.937ProArg: 0.937 ± 0.014	4.382ProSer: 4.382 ± 0.041	3.657ProThr: 3.657 ± 0.045	1.598ProVal: 1.598 ± 0.02	0.242ProTrp: 0.242 ± 0.006	1.228ProTyr: 1.228 ± 0.015	0.0ProXaa: 0.0 ± 0.0
Gln	1.198GlnAla: 1.198 ± 0.018	0.637GlnCys: 0.637 ± 0.012	1.761GlnAsp: 1.761 ± 0.022	2.426GlnGlu: 2.426 ± 0.026	2.088GlnPhe: 2.088 ± 0.02	1.251GlnGly: 1.251 ± 0.016	1.17GlnHis: 1.17 ± 0.023	3.371GlnIle: 3.371 ± 0.026	2.899GlnLys: 2.899 ± 0.024	4.628GlnLeu: 4.628 ± 0.047	0.785GlnMet: 0.785 ± 0.013	3.142GlnAsn: 3.142 ± 0.033	2.428GlnPro: 2.428 ± 0.039	11.464GlnGln: 11.464 ± 0.232	1.314GlnArg: 1.314 ± 0.018	3.951GlnSer: 3.951 ± 0.037	2.335GlnThr: 2.335 ± 0.026	1.736GlnVal: 1.736 ± 0.02	0.365GlnTrp: 0.365 ± 0.008	1.766GlnTyr: 1.766 ± 0.019	0.0GlnXaa: 0.0 ± 0.0
Arg	1.023ArgAla: 1.023 ± 0.014	0.445ArgCys: 0.445 ± 0.009	1.78ArgAsp: 1.78 ± 0.034	1.91ArgGlu: 1.91 ± 0.029	1.467ArgPhe: 1.467 ± 0.014	1.328ArgGly: 1.328 ± 0.019	0.475ArgHis: 0.475 ± 0.01	2.412ArgIle: 2.412 ± 0.018	2.31ArgLys: 2.31 ± 0.023	2.603ArgLeu: 2.603 ± 0.028	0.541ArgMet: 0.541 ± 0.01	2.057ArgAsn: 2.057 ± 0.02	0.952ArgPro: 0.952 ± 0.014	0.992ArgGln: 0.992 ± 0.014	1.07ArgArg: 1.07 ± 0.018	2.303ArgSer: 2.303 ± 0.029	1.232ArgThr: 1.232 ± 0.015	1.573ArgVal: 1.573 ± 0.018	0.258ArgTrp: 0.258 ± 0.007	1.155ArgTyr: 1.155 ± 0.013	0.0ArgXaa: 0.0 ± 0.0
Ser	2.624SerAla: 2.624 ± 0.027	1.216SerCys: 1.216 ± 0.023	4.129SerAsp: 4.129 ± 0.033	3.823SerGlu: 3.823 ± 0.034	4.708SerPhe: 4.708 ± 0.031	4.462SerGly: 4.462 ± 0.043	1.447SerHis: 1.447 ± 0.016	9.068SerIle: 9.068 ± 0.055	6.74SerLys: 6.74 ± 0.039	8.399SerLeu: 8.399 ± 0.053	1.512SerMet: 1.512 ± 0.019	10.062SerAsn: 10.062 ± 0.076	4.603SerPro: 4.603 ± 0.046	3.374SerGln: 3.374 ± 0.034	2.256SerArg: 2.256 ± 0.027	14.562SerSer: 14.562 ± 0.147	7.006SerThr: 7.006 ± 0.054	3.737SerVal: 3.737 ± 0.027	0.551SerTrp: 0.551 ± 0.009	2.719SerTyr: 2.719 ± 0.026	0.0SerXaa: 0.0 ± 0.0
Thr	2.018ThrAla: 2.018 ± 0.027	0.826ThrCys: 0.826 ± 0.015	2.317ThrAsp: 2.317 ± 0.026	2.394ThrGlu: 2.394 ± 0.022	2.646ThrPhe: 2.646 ± 0.025	2.397ThrGly: 2.397 ± 0.033	0.932ThrHis: 0.932 ± 0.013	5.506ThrIle: 5.506 ± 0.039	3.641ThrLys: 3.641 ± 0.027	4.907ThrLeu: 4.907 ± 0.034	0.845ThrMet: 0.845 ± 0.011	5.054ThrAsn: 5.054 ± 0.034	3.562ThrPro: 3.562 ± 0.052	2.16ThrGln: 2.16 ± 0.024	1.336ThrArg: 1.336 ± 0.014	6.027ThrSer: 6.027 ± 0.047	8.921ThrThr: 8.921 ± 0.123	2.419ThrVal: 2.419 ± 0.027	0.336ThrTrp: 0.336 ± 0.007	1.597ThrTyr: 1.597 ± 0.018	0.0ThrXaa: 0.0 ± 0.0
Val	1.791ValAla: 1.791 ± 0.024	0.798ValCys: 0.798 ± 0.014	2.516ValAsp: 2.516 ± 0.025	2.731ValGlu: 2.731 ± 0.026	2.114ValPhe: 2.114 ± 0.02	2.37ValGly: 2.37 ± 0.025	0.711ValHis: 0.711 ± 0.011	3.415ValIle: 3.415 ± 0.032	3.142ValLys: 3.142 ± 0.024	3.915ValLeu: 3.915 ± 0.027	0.722ValMet: 0.722 ± 0.011	3.196ValAsn: 3.196 ± 0.027	1.676ValPro: 1.676 ± 0.022	1.5ValGln: 1.5 ± 0.017	1.203ValArg: 1.203 ± 0.013	3.781ValSer: 3.781 ± 0.029	2.364ValThr: 2.364 ± 0.026	2.511ValVal: 2.511 ± 0.026	0.391ValTrp: 0.391 ± 0.011	1.591ValTyr: 1.591 ± 0.017	0.0ValXaa: 0.0 ± 0.0
Trp	0.275TrpAla: 0.275 ± 0.007	0.131TrpCys: 0.131 ± 0.004	0.487TrpAsp: 0.487 ± 0.01	0.428TrpGlu: 0.428 ± 0.009	0.405TrpPhe: 0.405 ± 0.008	0.37TrpGly: 0.37 ± 0.009	0.11TrpHis: 0.11 ± 0.004	0.655TrpIle: 0.655 ± 0.013	0.617TrpLys: 0.617 ± 0.011	0.623TrpLeu: 0.623 ± 0.012	0.17TrpMet: 0.17 ± 0.004	0.587TrpAsn: 0.587 ± 0.012	0.155TrpPro: 0.155 ± 0.005	0.168TrpGln: 0.168 ± 0.005	0.306TrpArg: 0.306 ± 0.007	0.639TrpSer: 0.639 ± 0.011	0.337TrpThr: 0.337 ± 0.007	0.424TrpVal: 0.424 ± 0.009	0.09TrpTrp: 0.09 ± 0.004	0.277TrpTyr: 0.277 ± 0.007	0.0TrpXaa: 0.0 ± 0.0
Tyr	0.96TyrAla: 0.96 ± 0.015	0.761TyrCys: 0.761 ± 0.024	1.981TyrAsp: 1.981 ± 0.022	1.751TyrGlu: 1.751 ± 0.019	2.117TyrPhe: 2.117 ± 0.021	1.673TyrGly: 1.673 ± 0.02	0.825TyrHis: 0.825 ± 0.013	2.645TyrIle: 2.645 ± 0.023	2.585TyrLys: 2.585 ± 0.025	3.551TyrLeu: 3.551 ± 0.028	0.527TyrMet: 0.527 ± 0.01	3.214TyrAsn: 3.214 ± 0.031	1.323TyrPro: 1.323 ± 0.017	1.902TyrGln: 1.902 ± 0.02	1.141TyrArg: 1.141 ± 0.015	3.383TyrSer: 3.383 ± 0.027	1.603TyrThr: 1.603 ± 0.017	1.294TyrVal: 1.294 ± 0.016	0.309TyrTrp: 0.309 ± 0.007	1.896TyrTyr: 1.896 ± 0.024	0.0TyrXaa: 0.0 ± 0.0
Xaa	0.0XaaAla: 0.0 ± 0.0	0.0XaaCys: 0.0 ± 0.0	0.0XaaAsp: 0.0 ± 0.0	0.0XaaGlu: 0.0 ± 0.0	0.0XaaPhe: 0.0 ± 0.0	0.0XaaGly: 0.0 ± 0.0	0.0XaaHis: 0.0 ± 0.0	0.0XaaIle: 0.0 ± 0.0	0.0XaaLys: 0.0 ± 0.0	0.0XaaLeu: 0.0 ± 0.0	0.0XaaMet: 0.0 ± 0.0	0.0XaaAsn: 0.0 ± 0.0	0.0XaaPro: 0.0 ± 0.0	0.0XaaGln: 0.0 ± 0.0	0.0XaaArg: 0.0 ± 0.0	0.0XaaSer: 0.0 ± 0.0	0.0XaaThr: 0.0 ± 0.0	0.0XaaVal: 0.0 ± 0.0	0.0XaaTrp: 0.0 ± 0.0	0.0XaaTyr: 0.0 ± 0.0	0.001XaaXaa: 0.001 ± 0.001

Statistics based on 12755 proteins (6860848 amino acids)

Note: The error has been estimated with the bootstraping (x100) at the protein level

Above dipeptide statistics (among other stats for this proteome) you can download from this CSV file

Reference: Kozlowski LP. Proteome-pI 2.0: Proteome Isoelectric Point Database Update. Nucleic Acids Res. 2021, doi: 10.1093/nar/gkab944

Contact: Lukasz P. Kozlowski

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