Amino acid dipepetide frequency for Rhizobacter sp. Root29

Apart from single amino acid frequencies one can also calculate so called amino acid dipeptide frequency.

There is 400 possibilites (441 if we consider Xaa as additional 21st amino acid representing all non-standard or unknown amino acids). Thus, if the dipeptides would be present randomly in proteins each amino acid dipeptide should be present with 0.25% frequency. As it is not the case in the nature, for better visablity all more than expected dipepetides are marked by red, and those which are underrepresented are marked by blue in the table.

All values are presented as per milles (‰), therefore need to be multiplied by 10^-3.

For more information see sequence space article on Wikipedia.

	Ala	Cys	Asp	Glu	Phe	Gly	His	Ile	Lys	Leu	Met	Asn	Pro	Gln	Arg	Ser	Thr	Val	Trp	Tyr	Xaa
Ala	19.917AlaAla: 19.917 ± 0.162	1.357AlaCys: 1.357 ± 0.033	7.174AlaAsp: 7.174 ± 0.065	6.462AlaGlu: 6.462 ± 0.059	4.171AlaPhe: 4.171 ± 0.047	11.54AlaGly: 11.54 ± 0.095	2.76AlaHis: 2.76 ± 0.043	4.946AlaIle: 4.946 ± 0.047	3.492AlaLys: 3.492 ± 0.057	16.025AlaLeu: 16.025 ± 0.14	3.493AlaMet: 3.493 ± 0.038	2.758AlaAsn: 2.758 ± 0.042	6.878AlaPro: 6.878 ± 0.093	6.006AlaGln: 6.006 ± 0.065	9.486AlaArg: 9.486 ± 0.08	7.209AlaSer: 7.209 ± 0.069	6.663AlaThr: 6.663 ± 0.069	9.624AlaVal: 9.624 ± 0.08	2.181AlaTrp: 2.181 ± 0.036	2.317AlaTyr: 2.317 ± 0.039	0.003AlaXaa: 0.003 ± 0.001
Cys	1.156CysAla: 1.156 ± 0.03	0.107CysCys: 0.107 ± 0.007	0.523CysAsp: 0.523 ± 0.017	0.504CysGlu: 0.504 ± 0.017	0.292CysPhe: 0.292 ± 0.014	0.948CysGly: 0.948 ± 0.03	0.269CysHis: 0.269 ± 0.013	0.355CysIle: 0.355 ± 0.014	0.2CysLys: 0.2 ± 0.01	0.803CysLeu: 0.803 ± 0.02	0.161CysMet: 0.161 ± 0.008	0.194CysAsn: 0.194 ± 0.009	0.423CysPro: 0.423 ± 0.015	0.221CysGln: 0.221 ± 0.01	0.619CysArg: 0.619 ± 0.016	0.511CysSer: 0.511 ± 0.018	0.481CysThr: 0.481 ± 0.016	0.699CysVal: 0.699 ± 0.018	0.117CysTrp: 0.117 ± 0.008	0.185CysTyr: 0.185 ± 0.011	0.0CysXaa: 0.0 ± 0.0
Asp	8.222AspAla: 8.222 ± 0.073	0.465AspCys: 0.465 ± 0.017	3.416AspAsp: 3.416 ± 0.057	3.472AspGlu: 3.472 ± 0.045	1.926AspPhe: 1.926 ± 0.036	5.498AspGly: 5.498 ± 0.062	1.143AspHis: 1.143 ± 0.024	2.194AspIle: 2.194 ± 0.035	1.581AspLys: 1.581 ± 0.032	5.43AspLeu: 5.43 ± 0.059	0.995AspMet: 0.995 ± 0.024	1.235AspAsn: 1.235 ± 0.03	3.108AspPro: 3.108 ± 0.039	1.588AspGln: 1.588 ± 0.036	3.563AspArg: 3.563 ± 0.045	2.394AspSer: 2.394 ± 0.039	2.765AspThr: 2.765 ± 0.053	4.057AspVal: 4.057 ± 0.043	1.008AspTrp: 1.008 ± 0.023	1.267AspTyr: 1.267 ± 0.023	0.001AspXaa: 0.001 ± 0.001
Glu	6.673GluAla: 6.673 ± 0.068	0.336GluCys: 0.336 ± 0.013	1.888GluAsp: 1.888 ± 0.034	1.944GluGlu: 1.944 ± 0.044	1.737GluPhe: 1.737 ± 0.029	3.284GluGly: 3.284 ± 0.052	1.285GluHis: 1.285 ± 0.028	2.31GluIle: 2.31 ± 0.037	1.415GluLys: 1.415 ± 0.033	6.033GluLeu: 6.033 ± 0.073	1.19GluMet: 1.19 ± 0.025	1.038GluAsn: 1.038 ± 0.026	2.739GluPro: 2.739 ± 0.042	2.35GluGln: 2.35 ± 0.037	4.955GluArg: 4.955 ± 0.064	2.383GluSer: 2.383 ± 0.039	2.347GluThr: 2.347 ± 0.033	3.87GluVal: 3.87 ± 0.05	0.645GluTrp: 0.645 ± 0.02	0.872GluTyr: 0.872 ± 0.023	0.002GluXaa: 0.002 ± 0.001
Phe	4.292PheAla: 4.292 ± 0.047	0.343PheCys: 0.343 ± 0.013	2.683PheAsp: 2.683 ± 0.035	2.08PheGlu: 2.08 ± 0.033	1.299PhePhe: 1.299 ± 0.028	3.428PheGly: 3.428 ± 0.05	0.752PheHis: 0.752 ± 0.02	1.453PheIle: 1.453 ± 0.03	1.041PheLys: 1.041 ± 0.023	2.707PheLeu: 2.707 ± 0.04	0.724PheMet: 0.724 ± 0.02	1.102PheAsn: 1.102 ± 0.025	1.419PhePro: 1.419 ± 0.033	0.985PheGln: 0.985 ± 0.022	1.985PheArg: 1.985 ± 0.031	2.101PheSer: 2.101 ± 0.036	1.933PheThr: 1.933 ± 0.03	2.749PheVal: 2.749 ± 0.039	0.5PheTrp: 0.5 ± 0.018	0.77PheTyr: 0.77 ± 0.024	0.0PheXaa: 0.0 ± 0.0
Gly	9.679GlyAla: 9.679 ± 0.073	0.904GlyCys: 0.904 ± 0.027	4.388GlyAsp: 4.388 ± 0.053	4.342GlyGlu: 4.342 ± 0.06	3.352GlyPhe: 3.352 ± 0.04	7.306GlyGly: 7.306 ± 0.095	1.976GlyHis: 1.976 ± 0.038	3.796GlyIle: 3.796 ± 0.046	2.797GlyLys: 2.797 ± 0.05	9.272GlyLeu: 9.272 ± 0.081	2.077GlyMet: 2.077 ± 0.036	2.121GlyAsn: 2.121 ± 0.05	3.265GlyPro: 3.265 ± 0.045	3.186GlyGln: 3.186 ± 0.046	6.131GlyArg: 6.131 ± 0.063	5.151GlySer: 5.151 ± 0.072	4.798GlyThr: 4.798 ± 0.063	6.68GlyVal: 6.68 ± 0.064	1.68GlyTrp: 1.68 ± 0.032	2.175GlyTyr: 2.175 ± 0.036	0.001GlyXaa: 0.001 ± 0.0
His	3.11HisAla: 3.11 ± 0.045	0.256HisCys: 0.256 ± 0.012	1.383HisAsp: 1.383 ± 0.026	1.23HisGlu: 1.23 ± 0.025	0.889HisPhe: 0.889 ± 0.023	2.306HisGly: 2.306 ± 0.035	0.619HisHis: 0.619 ± 0.02	0.78HisIle: 0.78 ± 0.02	0.475HisLys: 0.475 ± 0.016	2.344HisLeu: 2.344 ± 0.038	0.404HisMet: 0.404 ± 0.013	0.428HisAsn: 0.428 ± 0.015	1.502HisPro: 1.502 ± 0.029	0.731HisGln: 0.731 ± 0.021	1.64HisArg: 1.64 ± 0.027	0.974HisSer: 0.974 ± 0.024	0.954HisThr: 0.954 ± 0.022	1.592HisVal: 1.592 ± 0.03	0.422HisTrp: 0.422 ± 0.015	0.574HisTyr: 0.574 ± 0.018	0.0HisXaa: 0.0 ± 0.0
Ile	6.154IleAla: 6.154 ± 0.061	0.309IleCys: 0.309 ± 0.012	3.192IleAsp: 3.192 ± 0.041	2.887IleGlu: 2.887 ± 0.043	1.039IlePhe: 1.039 ± 0.026	4.15IleGly: 4.15 ± 0.045	0.795IleHis: 0.795 ± 0.022	1.074IleIle: 1.074 ± 0.027	1.158IleLys: 1.158 ± 0.029	2.625IleLeu: 2.625 ± 0.042	0.529IleMet: 0.529 ± 0.02	1.116IleAsn: 1.116 ± 0.027	1.688IlePro: 1.688 ± 0.031	1.059IleGln: 1.059 ± 0.024	2.353IleArg: 2.353 ± 0.036	2.033IleSer: 2.033 ± 0.029	2.196IleThr: 2.196 ± 0.035	3.469IleVal: 3.469 ± 0.041	0.35IleTrp: 0.35 ± 0.015	0.717IleTyr: 0.717 ± 0.022	0.001IleXaa: 0.001 ± 0.0
Lys	3.722LysAla: 3.722 ± 0.053	0.135LysCys: 0.135 ± 0.009	1.42LysAsp: 1.42 ± 0.033	1.251LysGlu: 1.251 ± 0.025	0.854LysPhe: 0.854 ± 0.021	2.175LysGly: 2.175 ± 0.044	0.581LysHis: 0.581 ± 0.017	1.128LysIle: 1.128 ± 0.03	1.116LysLys: 1.116 ± 0.032	3.318LysLeu: 3.318 ± 0.053	0.627LysMet: 0.627 ± 0.021	0.791LysAsn: 0.791 ± 0.023	1.934LysPro: 1.934 ± 0.04	1.1LysGln: 1.1 ± 0.023	2.088LysArg: 2.088 ± 0.032	1.502LysSer: 1.502 ± 0.032	1.69LysThr: 1.69 ± 0.032	2.242LysVal: 2.242 ± 0.039	0.305LysTrp: 0.305 ± 0.013	0.558LysTyr: 0.558 ± 0.019	0.001LysXaa: 0.001 ± 0.001
Leu	15.913LeuAla: 15.913 ± 0.126	0.962LeuCys: 0.962 ± 0.024	6.162LeuAsp: 6.162 ± 0.068	4.642LeuGlu: 4.642 ± 0.057	3.384LeuPhe: 3.384 ± 0.055	8.777LeuGly: 8.777 ± 0.078	2.444LeuHis: 2.444 ± 0.035	3.78LeuIle: 3.78 ± 0.045	3.562LeuLys: 3.562 ± 0.052	11.831LeuLeu: 11.831 ± 0.138	2.446LeuMet: 2.446 ± 0.038	2.61LeuAsn: 2.61 ± 0.038	6.484LeuPro: 6.484 ± 0.077	4.695LeuGln: 4.695 ± 0.05	8.51LeuArg: 8.51 ± 0.085	6.459LeuSer: 6.459 ± 0.06	5.357LeuThr: 5.357 ± 0.064	8.098LeuVal: 8.098 ± 0.082	1.468LeuTrp: 1.468 ± 0.03	1.983LeuTyr: 1.983 ± 0.034	0.002LeuXaa: 0.002 ± 0.001
Met	2.795MetAla: 2.795 ± 0.04	0.142MetCys: 0.142 ± 0.009	1.071MetAsp: 1.071 ± 0.025	0.89MetGlu: 0.89 ± 0.021	0.67MetPhe: 0.67 ± 0.016	1.596MetGly: 1.596 ± 0.028	0.499MetHis: 0.499 ± 0.018	0.786MetIle: 0.786 ± 0.02	0.925MetLys: 0.925 ± 0.023	2.602MetLeu: 2.602 ± 0.037	0.501MetMet: 0.501 ± 0.016	0.78MetAsn: 0.78 ± 0.021	1.478MetPro: 1.478 ± 0.028	1.001MetGln: 1.001 ± 0.027	1.721MetArg: 1.721 ± 0.026	1.529MetSer: 1.529 ± 0.028	1.559MetThr: 1.559 ± 0.029	1.587MetVal: 1.587 ± 0.031	0.194MetTrp: 0.194 ± 0.01	0.336MetTyr: 0.336 ± 0.014	0.001MetXaa: 0.001 ± 0.001
Asn	3.198AsnAla: 3.198 ± 0.043	0.23AsnCys: 0.23 ± 0.012	1.39AsnAsp: 1.39 ± 0.04	1.104AsnGlu: 1.104 ± 0.025	0.863AsnPhe: 0.863 ± 0.025	2.362AsnGly: 2.362 ± 0.045	0.49AsnHis: 0.49 ± 0.017	0.932AsnIle: 0.932 ± 0.025	0.651AsnLys: 0.651 ± 0.021	2.524AsnLeu: 2.524 ± 0.037	0.468AsnMet: 0.468 ± 0.017	0.671AsnAsn: 0.671 ± 0.024	1.707AsnPro: 1.707 ± 0.034	0.812AsnGln: 0.812 ± 0.021	1.537AsnArg: 1.537 ± 0.03	1.099AsnSer: 1.099 ± 0.024	1.312AsnThr: 1.312 ± 0.029	1.772AsnVal: 1.772 ± 0.036	0.373AsnTrp: 0.373 ± 0.015	0.596AsnTyr: 0.596 ± 0.018	0.001AsnXaa: 0.001 ± 0.001
Pro	7.896ProAla: 7.896 ± 0.096	0.347ProCys: 0.347 ± 0.014	3.33ProAsp: 3.33 ± 0.044	2.85ProGlu: 2.85 ± 0.041	1.79ProPhe: 1.79 ± 0.032	4.887ProGly: 4.887 ± 0.058	1.138ProHis: 1.138 ± 0.026	1.802ProIle: 1.802 ± 0.035	1.472ProLys: 1.472 ± 0.028	5.566ProLeu: 5.566 ± 0.062	1.354ProMet: 1.354 ± 0.028	1.228ProAsn: 1.228 ± 0.029	3.064ProPro: 3.064 ± 0.065	2.255ProGln: 2.255 ± 0.037	3.294ProArg: 3.294 ± 0.049	2.868ProSer: 2.868 ± 0.046	2.713ProThr: 2.713 ± 0.048	4.511ProVal: 4.511 ± 0.06	0.87ProTrp: 0.87 ± 0.019	1.049ProTyr: 1.049 ± 0.026	0.002ProXaa: 0.002 ± 0.001
Gln	6.083GlnAla: 6.083 ± 0.067	0.274GlnCys: 0.274 ± 0.013	1.485GlnAsp: 1.485 ± 0.027	1.278GlnGlu: 1.278 ± 0.029	1.226GlnPhe: 1.226 ± 0.03	2.992GlnGly: 2.992 ± 0.037	0.948GlnHis: 0.948 ± 0.02	1.509GlnIle: 1.509 ± 0.033	0.914GlnLys: 0.914 ± 0.022	4.356GlnLeu: 4.356 ± 0.061	0.912GlnMet: 0.912 ± 0.023	0.73GlnAsn: 0.73 ± 0.021	2.392GlnPro: 2.392 ± 0.04	2.115GlnGln: 2.115 ± 0.044	3.883GlnArg: 3.883 ± 0.053	1.911GlnSer: 1.911 ± 0.033	1.696GlnThr: 1.696 ± 0.035	3.374GlnVal: 3.374 ± 0.039	0.654GlnTrp: 0.654 ± 0.02	0.701GlnTyr: 0.701 ± 0.021	0.001GlnXaa: 0.001 ± 0.001
Arg	8.519ArgAla: 8.519 ± 0.078	0.677ArgCys: 0.677 ± 0.02	4.008ArgAsp: 4.008 ± 0.057	4.21ArgGlu: 4.21 ± 0.055	3.055ArgPhe: 3.055 ± 0.041	5.086ArgGly: 5.086 ± 0.056	2.069ArgHis: 2.069 ± 0.037	3.436ArgIle: 3.436 ± 0.042	1.792ArgLys: 1.792 ± 0.031	8.493ArgLeu: 8.493 ± 0.08	1.855ArgMet: 1.855 ± 0.031	1.7ArgAsn: 1.7 ± 0.035	3.504ArgPro: 3.504 ± 0.048	2.961ArgGln: 2.961 ± 0.045	5.802ArgArg: 5.802 ± 0.067	4.028ArgSer: 4.028 ± 0.049	3.29ArgThr: 3.29 ± 0.04	5.507ArgVal: 5.507 ± 0.061	1.387ArgTrp: 1.387 ± 0.029	1.761ArgTyr: 1.761 ± 0.034	0.002ArgXaa: 0.002 ± 0.001
Ser	6.857SerAla: 6.857 ± 0.061	0.458SerCys: 0.458 ± 0.017	2.712SerAsp: 2.712 ± 0.036	2.301SerGlu: 2.301 ± 0.038	2.225SerPhe: 2.225 ± 0.034	5.344SerGly: 5.344 ± 0.064	1.186SerHis: 1.186 ± 0.025	2.266SerIle: 2.266 ± 0.041	1.389SerLys: 1.389 ± 0.027	5.925SerLeu: 5.925 ± 0.056	1.274SerMet: 1.274 ± 0.029	1.339SerAsn: 1.339 ± 0.03	3.021SerPro: 3.021 ± 0.047	1.853SerGln: 1.853 ± 0.036	3.735SerArg: 3.735 ± 0.047	3.199SerSer: 3.199 ± 0.054	3.226SerThr: 3.226 ± 0.052	4.028SerVal: 4.028 ± 0.05	0.838SerTrp: 0.838 ± 0.021	1.197SerTyr: 1.197 ± 0.028	0.0SerXaa: 0.0 ± 0.0
Thr	6.267ThrAla: 6.267 ± 0.073	0.432ThrCys: 0.432 ± 0.015	2.665ThrAsp: 2.665 ± 0.043	2.292ThrGlu: 2.292 ± 0.031	1.494ThrPhe: 1.494 ± 0.028	4.702ThrGly: 4.702 ± 0.068	1.184ThrHis: 1.184 ± 0.027	1.879ThrIle: 1.879 ± 0.034	1.174ThrLys: 1.174 ± 0.028	6.672ThrLeu: 6.672 ± 0.067	1.117ThrMet: 1.117 ± 0.025	1.195ThrAsn: 1.195 ± 0.029	3.693ThrPro: 3.693 ± 0.044	1.993ThrGln: 1.993 ± 0.039	3.514ThrArg: 3.514 ± 0.038	2.722ThrSer: 2.722 ± 0.047	3.024ThrThr: 3.024 ± 0.059	4.3ThrVal: 4.3 ± 0.058	0.761ThrTrp: 0.761 ± 0.023	1.034ThrTyr: 1.034 ± 0.026	0.0ThrXaa: 0.0 ± 0.0
Val	10.007ValAla: 10.007 ± 0.073	0.677ValCys: 0.677 ± 0.019	4.533ValAsp: 4.533 ± 0.055	4.01ValGlu: 4.01 ± 0.048	2.717ValPhe: 2.717 ± 0.043	5.836ValGly: 5.836 ± 0.055	1.664ValHis: 1.664 ± 0.032	3.166ValIle: 3.166 ± 0.046	2.385ValLys: 2.385 ± 0.042	8.672ValLeu: 8.672 ± 0.082	1.795ValMet: 1.795 ± 0.032	2.039ValAsn: 2.039 ± 0.037	4.163ValPro: 4.163 ± 0.055	2.925ValGln: 2.925 ± 0.044	5.244ValArg: 5.244 ± 0.054	4.316ValSer: 4.316 ± 0.047	4.162ValThr: 4.162 ± 0.056	6.32ValVal: 6.32 ± 0.073	1.022ValTrp: 1.022 ± 0.026	1.493ValTyr: 1.493 ± 0.031	0.001ValXaa: 0.001 ± 0.001
Trp	1.489TrpAla: 1.489 ± 0.028	0.159TrpCys: 0.159 ± 0.009	0.669TrpAsp: 0.669 ± 0.017	0.522TrpGlu: 0.522 ± 0.017	0.58TrpPhe: 0.58 ± 0.017	1.025TrpGly: 1.025 ± 0.026	0.409TrpHis: 0.409 ± 0.014	0.666TrpIle: 0.666 ± 0.018	0.44TrpLys: 0.44 ± 0.016	2.141TrpLeu: 2.141 ± 0.04	0.4TrpMet: 0.4 ± 0.013	0.447TrpAsn: 0.447 ± 0.015	0.799TrpPro: 0.799 ± 0.019	0.828TrpGln: 0.828 ± 0.023	1.387TrpArg: 1.387 ± 0.028	0.937TrpSer: 0.937 ± 0.024	0.824TrpThr: 0.824 ± 0.024	1.099TrpVal: 1.099 ± 0.023	0.33TrpTrp: 0.33 ± 0.014	0.298TrpTyr: 0.298 ± 0.013	0.0TrpXaa: 0.0 ± 0.0
Tyr	2.512TyrAla: 2.512 ± 0.031	0.198TyrCys: 0.198 ± 0.01	1.233TyrAsp: 1.233 ± 0.032	1.022TyrGlu: 1.022 ± 0.027	0.78TyrPhe: 0.78 ± 0.021	1.834TyrGly: 1.834 ± 0.035	0.402TyrHis: 0.402 ± 0.014	0.635TyrIle: 0.635 ± 0.02	0.558TyrLys: 0.558 ± 0.019	2.225TyrLeu: 2.225 ± 0.04	0.34TyrMet: 0.34 ± 0.015	0.57TyrAsn: 0.57 ± 0.021	1.024TyrPro: 1.024 ± 0.024	0.772TyrGln: 0.772 ± 0.019	1.696TyrArg: 1.696 ± 0.033	1.048TyrSer: 1.048 ± 0.029	1.125TyrThr: 1.125 ± 0.023	1.565TyrVal: 1.565 ± 0.034	0.344TyrTrp: 0.344 ± 0.015	0.508TyrTyr: 0.508 ± 0.02	0.0TyrXaa: 0.0 ± 0.0
Xaa	0.002XaaAla: 0.002 ± 0.001	0.001XaaCys: 0.001 ± 0.001	0.001XaaAsp: 0.001 ± 0.001	0.001XaaGlu: 0.001 ± 0.001	0.0XaaPhe: 0.0 ± 0.0	0.001XaaGly: 0.001 ± 0.001	0.0XaaHis: 0.0 ± 0.0	0.001XaaIle: 0.001 ± 0.001	0.001XaaLys: 0.001 ± 0.001	0.001XaaLeu: 0.001 ± 0.001	0.0XaaMet: 0.0 ± 0.0	0.0XaaAsn: 0.0 ± 0.0	0.001XaaPro: 0.001 ± 0.001	0.0XaaGln: 0.0 ± 0.0	0.002XaaArg: 0.002 ± 0.001	0.001XaaSer: 0.001 ± 0.001	0.001XaaThr: 0.001 ± 0.001	0.002XaaVal: 0.002 ± 0.001	0.001XaaTrp: 0.001 ± 0.001	0.0XaaTyr: 0.0 ± 0.0	0.004XaaXaa: 0.004 ± 0.002

Statistics based on 5611 proteins (1878270 amino acids)

Note: The error has been estimated with the bootstraping (x100) at the protein level

Above dipeptide statistics (among other stats for this proteome) you can download from this CSV file

Reference: Kozlowski LP. Proteome-pI 2.0: Proteome Isoelectric Point Database Update. Nucleic Acids Res. 2021, doi: 10.1093/nar/gkab944

Contact: Lukasz P. Kozlowski

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