candidate division MSBL1 archaeon SCGC-AAA259D18

Taxonomy: cellular organisms; Archaea; Euryarchaeota; Euryarchaeota incertae sedis; candidate division MSBL1

Average proteome isoelectric point is 6.18

Get precalculated fractions of proteins

Acidic
pI < 6.8
6.8-7.4
pI > 7.4
Basic
    
All



Virtual 2D-PAGE plot for 389 proteins (isoelectric point calculated using IPC2_protein)

Get csv file with sequences according to given criteria:
        -     Method 

     -  kDa    
                                                                                      

* You can choose from 21 different methods for calculating isoelectric point

Summary statistics related to proteome-wise predictions


    
Protein with the lowest isoelectric point:
>tr|A0A133UCI9|A0A133UCI9_9EURY Uncharacterized protein OS=candidate division MSBL1 archaeon SCGC-AAA259D18 OX=1698262 GN=AKJ63_00650 PE=4 SV=1
MM1 pKa = 7.16GASRR5 pKa = 11.84FPPLLPLVNFFSGLTGVCLFGFLSLLVEE33 pKa = 4.61AEE35 pKa = 4.01AADD38 pKa = 3.91VGVGTYY44 pKa = 10.15SFEE47 pKa = 4.04FFSALFRR54 pKa = 11.84DD55 pKa = 4.98FAYY58 pKa = 11.0LGMYY62 pKa = 9.35TPFSFEE68 pKa = 3.8NTVV71 pKa = 2.77

Molecular weight: 7.79 kDa
Isoelectric point according different methods:





Protein with the highest isoelectric point:
>tr|A0A133UCD3|A0A133UCD3_9EURY Uncharacterized protein OS=candidate division MSBL1 archaeon SCGC-AAA259D18 OX=1698262 GN=AKJ63_00660 PE=4 SV=1
MM1 pKa = 7.76LEE3 pKa = 4.0ATEE6 pKa = 4.03LTRR9 pKa = 11.84VVVNKK14 pKa = 10.42SVVPEE19 pKa = 3.53RR20 pKa = 11.84RR21 pKa = 11.84GRR23 pKa = 11.84GRR25 pKa = 11.84RR26 pKa = 11.84GYY28 pKa = 10.2GRR30 pKa = 11.84RR31 pKa = 11.84PAVRR35 pKa = 11.84LLVYY39 pKa = 10.35AQLKK43 pKa = 9.3GIHH46 pKa = 6.49RR47 pKa = 11.84DD48 pKa = 3.2ARR50 pKa = 11.84LVKK53 pKa = 10.09HH54 pKa = 5.9LRR56 pKa = 11.84KK57 pKa = 9.88NPEE60 pKa = 3.77VAEE63 pKa = 4.11SLGLNGIPDD72 pKa = 3.68RR73 pKa = 11.84TTIGRR78 pKa = 11.84WRR80 pKa = 11.84KK81 pKa = 8.82RR82 pKa = 11.84LSRR85 pKa = 11.84VFKK88 pKa = 10.75KK89 pKa = 10.58AFQKK93 pKa = 10.59LSEE96 pKa = 4.26YY97 pKa = 11.04VQMLVPTEE105 pKa = 3.96DD106 pKa = 4.97LIVDD110 pKa = 4.11STPLEE115 pKa = 4.7DD116 pKa = 5.13YY117 pKa = 10.54RR118 pKa = 11.84DD119 pKa = 3.77PDD121 pKa = 4.08SRR123 pKa = 11.84WGKK126 pKa = 9.47YY127 pKa = 7.1SRR129 pKa = 11.84EE130 pKa = 3.75WFKK133 pKa = 11.22GFKK136 pKa = 10.22GHH138 pKa = 7.22FSVNQLGLPLKK149 pKa = 10.05TKK151 pKa = 8.46VTTGNRR157 pKa = 11.84HH158 pKa = 6.58DD159 pKa = 4.56SPFLPEE165 pKa = 5.39LISGLGSNRR174 pKa = 11.84VLADD178 pKa = 3.2AGYY181 pKa = 10.91DD182 pKa = 3.37SRR184 pKa = 11.84SNRR187 pKa = 11.84KK188 pKa = 8.73ACRR191 pKa = 3.3

Molecular weight: 21.9 kDa
Isoelectric point according different methods:





Peptides (in silico digests for buttom-up proteomics)

Below you can find in silico digests of the whole proteome with Trypsin, Chymotrypsin, Trypsin+LysC, LysN, ArgC proteases suitable for different mass spec machines.

Try
ESI
ChTry
ESI
ArgC
ESI
LysN
ESI
TryLysC
ESI

Try
MALDI
ChTry
MALDI
ArgC
MALDI
LysN
MALDI
TryLysC
MALDI

Try
LTQ
ChTry
LTQ
ArgC
LTQ
LysN
LTQ
TryLysC
LTQ

Try
MSlow
ChTry
MSlow
ArgC
MSlow
LysN
MSlow
TryLysC
MSlow

Try
MShigh
ChTry
MShigh
ArgC
MShigh
LysN
MShigh
TryLysC
MShigh

General Statistics

Number of major isoforms

Number of additional isoforms

Number of all proteins

Number of amino acids

Min. Seq. Length

Max. Seq. Length

Avg. Seq. Length

Avg. Mol. Weight

389

0

389

88468

59

999

227.4

25.5

Amino acid frequency

Ala

Cys

Asp

Glu

Phe

Gly

His

Ile

Lys

Leu

6.261 ± 0.127

0.956 ± 0.051

5.573 ± 0.12

9.939 ± 0.182

3.961 ± 0.092

7.917 ± 0.115

1.716 ± 0.046

6.727 ± 0.126

6.958 ± 0.14

9.197 ± 0.142

Met

Asn

Gln

Pro

Arg

Ser

Thr

Val

Trp

Tyr

2.245 ± 0.052

3.506 ± 0.093

4.232 ± 0.08

2.2 ± 0.053

5.752 ± 0.125

6.757 ± 0.1

4.777 ± 0.121

7.208 ± 0.118

1.219 ± 0.055

2.899 ± 0.075
Note: For amino acid frequency statistics the error has been estimated with the bootstraping (x100) at the protein level

Most of the basic statistics you can see at this page can be downloaded from this CSV file

For dipeptide frequency statistics click here
See this proteome in: uniprot_link
Proteome-pI is available under Creative Commons Attribution-NoDerivs license, for more details see here

Reference: Kozlowski LP. Proteome-pI 2.0: Proteome Isoelectric Point Database Update. Nucleic Acids Res. 2021, doi: 10.1093/nar/gkab944 Contact: Lukasz P. Kozlowski