Rattus norvegicus papillomavirus 3
Average proteome isoelectric point is 6.85
Get precalculated fractions of proteins
Acidic
pI < 6.8
6.8-7.4
pI > 7.4
Basic
All
Note: above files contain also dissociation constants (pKa)
Virtual 2D-PAGE plot for 5 proteins (isoelectric point calculated using IPC2_protein)
Get csv file with sequences according to given criteria:
* You can choose from 21 different methods for calculating isoelectric point
Summary statistics related to proteome-wise predictions
Protein with the lowest isoelectric point:
>tr|A0A0S2LV84|A0A0S2LV84_MNPV Replication protein E1 OS=Rattus norvegicus papillomavirus 3 OX=1756445 GN=E1 PE=3 SV=1
MM1 pKa = 7.71 IGPEE5 pKa = 4.18 TNRR8 pKa = 11.84 TLPNDD13 pKa = 3.92 PDD15 pKa = 4.3 LVSLYY20 pKa = 10.26 CDD22 pKa = 3.03 EE23 pKa = 4.38 VLEE26 pKa = 4.73 DD27 pKa = 4.05 EE28 pKa = 5.29 PIEE31 pKa = 4.52 AEE33 pKa = 4.1 QSQEE37 pKa = 3.98 EE38 pKa = 4.03 PSIIYY43 pKa = 9.82 RR44 pKa = 11.84 VLIEE48 pKa = 4.66 CPKK51 pKa = 9.58 CDD53 pKa = 3.85 RR54 pKa = 11.84 IIRR57 pKa = 11.84 LLCSADD63 pKa = 3.29 TASIRR68 pKa = 11.84 GLEE71 pKa = 3.87 LLLLGGLQIICPRR84 pKa = 11.84 CAEE87 pKa = 4.0 KK88 pKa = 10.43 HH89 pKa = 5.1 GRR91 pKa = 11.84 PP92 pKa = 3.78
Molecular weight: 10.29 kDa
Isoelectric point according different methods:
IPC2.protein.svr19 4.403
IPC2_protein 4.533
IPC_protein 4.393
Toseland 4.24
ProMoST 4.495
Dawson 4.329
Bjellqvist 4.482
Wikipedia 4.19
Rodwell 4.228
Grimsley 4.151
Solomon 4.317
Lehninger 4.279
Nozaki 4.444
DTASelect 4.558
Thurlkill 4.24
EMBOSS 4.202
Sillero 4.495
Patrickios 3.3
IPC_peptide 4.329
IPC2_peptide 4.495
IPC2.peptide.svr19 4.437
Protein with the highest isoelectric point:
>tr|A0A0S2LV87|A0A0S2LV87_MNPV Regulatory protein E2 OS=Rattus norvegicus papillomavirus 3 OX=1756445 GN=E2 PE=3 SV=1
MM1 pKa = 7.71 EE2 pKa = 5.02 SLCSRR7 pKa = 11.84 LSAVQEE13 pKa = 3.99 EE14 pKa = 4.78 LMCIYY19 pKa = 10.74 EE20 pKa = 4.28 EE21 pKa = 4.55 GDD23 pKa = 3.18 RR24 pKa = 11.84 TLATQVRR31 pKa = 11.84 HH32 pKa = 4.56 WQLIRR37 pKa = 11.84 QEE39 pKa = 4.15 QVLLHH44 pKa = 6.82 AARR47 pKa = 11.84 QQGHH51 pKa = 5.25 TRR53 pKa = 11.84 IGMQAVPSLAASQANAKK70 pKa = 9.65 NAIEE74 pKa = 3.98 MHH76 pKa = 6.77 LLLQSLLQSPYY87 pKa = 11.13 AKK89 pKa = 10.42 EE90 pKa = 3.66 PWTLSEE96 pKa = 4.13 TSRR99 pKa = 11.84 DD100 pKa = 3.64 LYY102 pKa = 10.82 LAPPSHH108 pKa = 6.09 TLKK111 pKa = 10.86 KK112 pKa = 10.05 SGQTIEE118 pKa = 3.99 VLFDD122 pKa = 3.77 GDD124 pKa = 4.53 EE125 pKa = 4.33 NNSMQYY131 pKa = 9.58 TKK133 pKa = 9.95 WLQVFYY139 pKa = 10.79 TDD141 pKa = 5.76 DD142 pKa = 3.51 RR143 pKa = 11.84 GMWKK147 pKa = 9.99 RR148 pKa = 11.84 VPSVCDD154 pKa = 3.08 ATGIFFDD161 pKa = 4.26 RR162 pKa = 11.84 NGHH165 pKa = 5.09 RR166 pKa = 11.84 EE167 pKa = 3.86 YY168 pKa = 10.97 YY169 pKa = 10.51 VRR171 pKa = 11.84 FEE173 pKa = 4.11 KK174 pKa = 10.72 EE175 pKa = 3.42 AMRR178 pKa = 11.84 FSVTGTWQAVDD189 pKa = 3.16 EE190 pKa = 4.49 GKK192 pKa = 10.19 IYY194 pKa = 10.82 SSVVPVASSTPTTGPPRR211 pKa = 11.84 GPAVHH216 pKa = 7.29 PDD218 pKa = 3.35 GVSATGHH225 pKa = 6.16 TSPNWDD231 pKa = 3.92 CSRR234 pKa = 11.84 HH235 pKa = 5.51 SPLPSGGGEE244 pKa = 3.84 DD245 pKa = 3.12 SGEE248 pKa = 4.03 EE249 pKa = 4.22 EE250 pKa = 4.44 KK251 pKa = 11.25 VHH253 pKa = 5.74 TRR255 pKa = 11.84 PEE257 pKa = 4.37 YY258 pKa = 10.12 TDD260 pKa = 3.32 HH261 pKa = 7.08 SYY263 pKa = 11.19 NRR265 pKa = 11.84 QTSPPSFRR273 pKa = 11.84 GEE275 pKa = 3.67 QSVGGEE281 pKa = 3.85 QHH283 pKa = 6.71 RR284 pKa = 11.84 EE285 pKa = 3.49 RR286 pKa = 11.84 ARR288 pKa = 11.84 GLGVPAGDD296 pKa = 3.42 VPEE299 pKa = 4.39 VPRR302 pKa = 11.84 AADD305 pKa = 3.71 KK306 pKa = 7.79 EE307 pKa = 4.86 TKK309 pKa = 10.39 SPEE312 pKa = 4.69 PISKK316 pKa = 10.16 RR317 pKa = 11.84 GPGRR321 pKa = 11.84 PRR323 pKa = 11.84 KK324 pKa = 9.49 GPSAAGEE331 pKa = 4.27 TTTDD335 pKa = 3.37 GEE337 pKa = 4.36 PRR339 pKa = 11.84 SKK341 pKa = 10.42 KK342 pKa = 9.88 RR343 pKa = 11.84 PRR345 pKa = 11.84 DD346 pKa = 3.27 RR347 pKa = 11.84 TGGYY351 pKa = 7.46 RR352 pKa = 11.84 TRR354 pKa = 11.84 KK355 pKa = 8.91 RR356 pKa = 11.84 RR357 pKa = 11.84 RR358 pKa = 11.84 TKK360 pKa = 10.2 RR361 pKa = 11.84 WATPPRR367 pKa = 11.84 EE368 pKa = 4.04 RR369 pKa = 11.84 GPDD372 pKa = 3.48 HH373 pKa = 7.4 VDD375 pKa = 3.69 LNATNKK381 pKa = 10.32 NEE383 pKa = 3.71 GRR385 pKa = 11.84 PNGPVNCSPPTPNPPDD401 pKa = 3.8 LSAIPPTPGVRR412 pKa = 11.84 VAGQSTPAGTSRR424 pKa = 11.84 APPTLVAEE432 pKa = 4.69 PDD434 pKa = 3.35 AGRR437 pKa = 11.84 TPAAAAAGLRR447 pKa = 11.84 SATPGSTARR456 pKa = 11.84 IRR458 pKa = 11.84 RR459 pKa = 11.84 PRR461 pKa = 11.84 VGPGPPCLLIIKK473 pKa = 7.66 GTPNGVKK480 pKa = 10.26 CLRR483 pKa = 11.84 YY484 pKa = 9.41 RR485 pKa = 11.84 LKK487 pKa = 9.26 TQHH490 pKa = 7.0 PSTFQFVSTTWQWVPATGSSRR511 pKa = 11.84 VGRR514 pKa = 11.84 ARR516 pKa = 11.84 ILVMCSDD523 pKa = 3.33 QTQRR527 pKa = 11.84 NIFLDD532 pKa = 3.89 TVKK535 pKa = 10.22 IPAGMTVEE543 pKa = 4.2 QCSMLAII550 pKa = 4.49
Molecular weight: 60.28 kDa
Isoelectric point according different methods:
IPC2.protein.svr19 9.0
IPC2_protein 8.931
IPC_protein 8.946
Toseland 9.619
ProMoST 9.414
Dawson 9.882
Bjellqvist 9.648
Wikipedia 10.058
Rodwell 10.116
Grimsley 9.955
Solomon 9.926
Lehninger 9.882
Nozaki 9.794
DTASelect 9.589
Thurlkill 9.736
EMBOSS 10.058
Sillero 9.853
Patrickios 5.626
IPC_peptide 9.911
IPC2_peptide 8.507
IPC2.peptide.svr19 7.917
Peptides (in silico digests for buttom-up proteomics)
Below you can find
in silico digests of the whole proteome with Trypsin, Chymotrypsin, Trypsin+LysC, LysN, ArgC proteases suitable for different mass spec machines.
Try ESI
ChTry ESI
ArgC ESI
LysN ESI
TryLysC ESI
Try MALDI
ChTry MALDI
ArgC MALDI
LysN MALDI
TryLysC MALDI
Try LTQ
ChTry LTQ
ArgC LTQ
LysN LTQ
TryLysC LTQ
Try MSlow
ChTry MSlow
ArgC MSlow
LysN MSlow
TryLysC MSlow
Try MShigh
ChTry MShigh
ArgC MShigh
LysN MShigh
TryLysC MShigh
General Statistics
Number of major isoforms
Number of additional isoforms
Number of all proteins
Number of amino acids
Min. Seq. Length
Max. Seq. Length
Avg. Seq. Length
Avg. Mol. Weight
5
0
5
1876
92
600
375.2
42.27
Amino acid frequency
Ala
Cys
Asp
Glu
Phe
Gly
His
Ile
Lys
Leu
6.237 ± 0.716
2.719 ± 0.662
5.384 ± 0.509
6.397 ± 0.445
3.625 ± 0.711
6.716 ± 0.72
1.972 ± 0.186
3.945 ± 0.55
4.797 ± 0.498
8.102 ± 0.844
Met
Asn
Gln
Pro
Arg
Ser
Thr
Val
Trp
Tyr
1.972 ± 0.308
4.211 ± 0.645
6.61 ± 1.228
4.478 ± 0.218
7.729 ± 0.921
7.409 ± 0.3
6.61 ± 0.566
6.183 ± 0.442
1.652 ± 0.208
3.252 ± 0.56
Note: For amino acid frequency statistics the error has been estimated with the bootstraping (x100) at the protein level
Most of the basic statistics you can see at this page can be downloaded from this CSV file
For dipeptide frequency statistics click here