Drosophila innubila nudivirus
Average proteome isoelectric point is 6.63
Get precalculated fractions of proteins
Acidic
pI < 6.8
6.8-7.4
pI > 7.4
Basic
All
Note: above files contain also dissociation constants (pKa)
Virtual 2D-PAGE plot for 106 proteins (isoelectric point calculated using IPC2_protein)
Get csv file with sequences according to given criteria:
* You can choose from 21 different methods for calculating isoelectric point
Summary statistics related to proteome-wise predictions
Protein with the lowest isoelectric point:
>tr|A0A2H4UXE5|A0A2H4UXE5_9VIRU Ac146-like protein OS=Drosophila innubila nudivirus OX=2057187 GN=DiNV_CH01M_ORF96 PE=4 SV=1
MM1 pKa = 7.75 FDD3 pKa = 2.97 GCLVVFVVVVANVVVVVFGFIVVDD27 pKa = 3.56 VVNVVVVVFGFIVVVVDD44 pKa = 3.54 VVDD47 pKa = 3.88 VVDD50 pKa = 3.84 VVVVVVGGDD59 pKa = 3.32 GGGGFVDD66 pKa = 4.31 VINFVVVDD74 pKa = 4.64 CIGVVCNCVVAAICDD89 pKa = 3.55 AATVLSVVLVLVLVLIIDD107 pKa = 4.11 GVVVVVICDD116 pKa = 3.27 AATVLSVVLVLVLVLIVDD134 pKa = 4.27 GVVVVVVEE142 pKa = 5.49 FIIGTFTVVALLFKK156 pKa = 10.12 FSIVISLFSAGSIIGVVGFAIEE178 pKa = 4.07 VDD180 pKa = 4.08 VVALFFFLFRR190 pKa = 11.84 CC191 pKa = 4.07
Molecular weight: 19.69 kDa
Isoelectric point according different methods:
IPC2.protein.svr19 4.224
IPC2_protein 3.223
IPC_protein 3.185
Toseland 2.982
ProMoST 3.439
Dawson 3.236
Bjellqvist 3.414
Wikipedia 3.261
Rodwell 3.045
Grimsley 2.905
Solomon 3.16
Lehninger 3.121
Nozaki 3.389
DTASelect 3.643
Thurlkill 3.083
EMBOSS 3.261
Sillero 3.338
Patrickios 0.006
IPC_peptide 3.147
IPC2_peptide 3.287
IPC2.peptide.svr19 3.698
Protein with the highest isoelectric point:
>tr|A0A2H4UXB1|A0A2H4UXB1_9VIRU P74 OS=Drosophila innubila nudivirus OX=2057187 GN=DiNV_CH01M_ORF67 PE=4 SV=1
MM1 pKa = 7.23 MMMILKK7 pKa = 7.97 MIPLLLLVVVAVAVIVIPVVAIVKK31 pKa = 7.23 MTIFLMTTPMMTMMTTMMMMRR52 pKa = 11.84 RR53 pKa = 11.84 RR54 pKa = 11.84 MKK56 pKa = 9.68 MMAIVTILKK65 pKa = 10.23 RR66 pKa = 11.84 KK67 pKa = 8.65 IIPWKK72 pKa = 8.97 MILMVQMIVYY82 pKa = 8.09 MIYY85 pKa = 10.12 MNDD88 pKa = 2.74 IFNVQKK94 pKa = 10.65 RR95 pKa = 11.84 ICIKK99 pKa = 10.75 NLILLEE105 pKa = 3.9 TRR107 pKa = 11.84 ICHH110 pKa = 5.77 ILFRR114 pKa = 11.84 SLMVSMLSTIKK125 pKa = 10.31 CVRR128 pKa = 11.84 ILTLRR133 pKa = 11.84 NLIEE137 pKa = 4.42 MLHH140 pKa = 6.36 CLNITLYY147 pKa = 10.87 VITWMLQNLQLIYY160 pKa = 11.06
Molecular weight: 19.01 kDa
Isoelectric point according different methods:
IPC2.protein.svr19 9.332
IPC2_protein 9.853
IPC_protein 10.423
Toseland 10.716
ProMoST 10.35
Dawson 10.818
Bjellqvist 10.496
Wikipedia 10.994
Rodwell 11.228
Grimsley 10.862
Solomon 10.891
Lehninger 10.862
Nozaki 10.716
DTASelect 10.482
Thurlkill 10.716
EMBOSS 11.111
Sillero 10.745
Patrickios 10.965
IPC_peptide 10.891
IPC2_peptide 9.589
IPC2.peptide.svr19 8.433
Peptides (in silico digests for buttom-up proteomics)
Below you can find
in silico digests of the whole proteome with Trypsin, Chymotrypsin, Trypsin+LysC, LysN, ArgC proteases suitable for different mass spec machines.
Try ESI
ChTry ESI
ArgC ESI
LysN ESI
TryLysC ESI
Try MALDI
ChTry MALDI
ArgC MALDI
LysN MALDI
TryLysC MALDI
Try LTQ
ChTry LTQ
ArgC LTQ
LysN LTQ
TryLysC LTQ
Try MSlow
ChTry MSlow
ArgC MSlow
LysN MSlow
TryLysC MSlow
Try MShigh
ChTry MShigh
ArgC MShigh
LysN MShigh
TryLysC MShigh
General Statistics
Number of major isoforms
Number of additional isoforms
Number of all proteins
Number of amino acids
Min. Seq. Length
Max. Seq. Length
Avg. Seq. Length
Avg. Mol. Weight
106
0
106
37911
50
1549
357.7
40.95
Amino acid frequency
Ala
Cys
Asp
Glu
Phe
Gly
His
Ile
Lys
Leu
4.162 ± 0.134
1.97 ± 0.097
6.455 ± 0.269
4.795 ± 0.127
4.205 ± 0.137
3.366 ± 0.212
2.044 ± 0.098
9.174 ± 0.227
6.61 ± 0.159
8.05 ± 0.216
Met
Asn
Gln
Pro
Arg
Ser
Thr
Val
Trp
Tyr
2.78 ± 0.108
9.451 ± 0.211
3.917 ± 0.131
3.925 ± 0.263
3.827 ± 0.129
7.966 ± 0.235
7.09 ± 0.278
4.977 ± 0.228
0.578 ± 0.048
4.656 ± 0.194
Note: For amino acid frequency statistics the error has been estimated with the bootstraping (x100) at the protein level
Most of the basic statistics you can see at this page can be downloaded from this CSV file
For dipeptide frequency statistics click here