Streptococcus phage Javan224

Taxonomy: Viruses; Duplodnaviria; Heunggongvirae; Uroviricota; Caudoviricetes; Caudovirales; Siphoviridae; unclassified Siphoviridae

Average proteome isoelectric point is 6.04

Get precalculated fractions of proteins

Acidic
pI < 6.8
6.8-7.4
pI > 7.4
Basic
    
All



Virtual 2D-PAGE plot for 59 proteins (isoelectric point calculated using IPC2_protein)

Get csv file with sequences according to given criteria:
        -     Method 

     -  kDa    
                                                                                      

* You can choose from 21 different methods for calculating isoelectric point

Summary statistics related to proteome-wise predictions


    
Protein with the lowest isoelectric point:
>tr|A0A4D6AVA0|A0A4D6AVA0_9CAUD Uncharacterized protein OS=Streptococcus phage Javan224 OX=2548054 GN=Javan224_0039 PE=4 SV=1
MM1 pKa = 7.62LGIKK5 pKa = 10.12LVDD8 pKa = 3.29VDD10 pKa = 3.66TSNARR15 pKa = 11.84TEE17 pKa = 4.06QTGTCEE23 pKa = 4.5LCFGTQWCDD32 pKa = 3.03NPILIFEE39 pKa = 4.88KK40 pKa = 10.41PDD42 pKa = 3.43GEE44 pKa = 4.25QIRR47 pKa = 11.84AEE49 pKa = 4.12GFGWDD54 pKa = 2.8WGDD57 pKa = 3.34YY58 pKa = 10.95HH59 pKa = 7.12EE60 pKa = 5.51IKK62 pKa = 10.0IDD64 pKa = 3.38NYY66 pKa = 11.29LNFSDD71 pKa = 3.95WFSKK75 pKa = 10.86KK76 pKa = 10.41DD77 pKa = 3.42INCEE81 pKa = 3.74RR82 pKa = 11.84LEE84 pKa = 3.78YY85 pKa = 10.5DD86 pKa = 3.03YY87 pKa = 10.79WYY89 pKa = 10.98LEE91 pKa = 4.22NLVEE95 pKa = 4.44WYY97 pKa = 10.47YY98 pKa = 11.21EE99 pKa = 3.89EE100 pKa = 4.65TIGYY104 pKa = 10.23DD105 pKa = 3.45DD106 pKa = 3.96

Molecular weight: 12.73 kDa
Isoelectric point according different methods:





Protein with the highest isoelectric point:
>tr|A0A4D6AZ13|A0A4D6AZ13_9CAUD Uncharacterized protein OS=Streptococcus phage Javan224 OX=2548054 GN=Javan224_0052 PE=4 SV=1
MM1 pKa = 7.33SFFVRR6 pKa = 11.84TKK8 pKa = 9.05TDD10 pKa = 2.54ISRR13 pKa = 11.84VEE15 pKa = 4.15KK16 pKa = 10.61KK17 pKa = 10.58VSNDD21 pKa = 3.2NILKK25 pKa = 10.34GKK27 pKa = 9.88RR28 pKa = 11.84ALANQVLLDD37 pKa = 3.83ADD39 pKa = 3.79KK40 pKa = 11.21YY41 pKa = 10.78IPKK44 pKa = 9.89KK45 pKa = 10.76DD46 pKa = 3.3GALRR50 pKa = 11.84ASGQIAIDD58 pKa = 3.89GSNVSWNTVYY68 pKa = 10.92ARR70 pKa = 11.84AQYY73 pKa = 10.53YY74 pKa = 6.63GTNGIVTFNHH84 pKa = 5.49YY85 pKa = 6.57TTPGTGKK92 pKa = 10.14LWYY95 pKa = 8.47DD96 pKa = 3.86TAQKK100 pKa = 11.02ANSDD104 pKa = 2.77KK105 pKa = 10.2WKK107 pKa = 10.3RR108 pKa = 11.84VAAKK112 pKa = 10.63GMGLL116 pKa = 3.45

Molecular weight: 12.88 kDa
Isoelectric point according different methods:





Peptides (in silico digests for buttom-up proteomics)

Below you can find in silico digests of the whole proteome with Trypsin, Chymotrypsin, Trypsin+LysC, LysN, ArgC proteases suitable for different mass spec machines.

Try
ESI
ChTry
ESI
ArgC
ESI
LysN
ESI
TryLysC
ESI

Try
MALDI
ChTry
MALDI
ArgC
MALDI
LysN
MALDI
TryLysC
MALDI

Try
LTQ
ChTry
LTQ
ArgC
LTQ
LysN
LTQ
TryLysC
LTQ

Try
MSlow
ChTry
MSlow
ArgC
MSlow
LysN
MSlow
TryLysC
MSlow

Try
MShigh
ChTry
MShigh
ArgC
MShigh
LysN
MShigh
TryLysC
MShigh

General Statistics

Number of major isoforms

Number of additional isoforms

Number of all proteins

Number of amino acids

Min. Seq. Length

Max. Seq. Length

Avg. Seq. Length

Avg. Mol. Weight

59

0

59

11901

42

1356

201.7

22.7

Amino acid frequency

Ala

Cys

Asp

Glu

Phe

Gly

His

Ile

Lys

Leu

7.436 ± 0.853

0.471 ± 0.11

6.537 ± 0.408

7.067 ± 0.66

3.949 ± 0.207

6.159 ± 0.526

1.134 ± 0.132

6.999 ± 0.312

8.268 ± 0.633

7.991 ± 0.295

Met

Asn

Gln

Pro

Arg

Ser

Thr

Val

Trp

Tyr

2.546 ± 0.157

5.949 ± 0.307

2.588 ± 0.185

4.504 ± 0.23

3.706 ± 0.257

6.571 ± 0.523

6.705 ± 0.602

6.201 ± 0.282

1.059 ± 0.133

4.159 ± 0.389
Note: For amino acid frequency statistics the error has been estimated with the bootstraping (x100) at the protein level

Most of the basic statistics you can see at this page can be downloaded from this CSV file

For dipeptide frequency statistics click here
See this proteome in: uniprot_link
Proteome-pI is available under Creative Commons Attribution-NoDerivs license, for more details see here

Reference: Kozlowski LP. Proteome-pI 2.0: Proteome Isoelectric Point Database Update. Nucleic Acids Res. 2021, doi: 10.1093/nar/gkab944 Contact: Lukasz P. Kozlowski