Amino acid dipepetide frequency for Lactococcus lactis subsp. lactis (strain IL1403) (Streptococcus lactis)

Apart from single amino acid frequencies one can also calculate so called amino acid dipeptide frequency.

There is 400 possibilites (441 if we consider Xaa as additional 21st amino acid representing all non-standard or unknown amino acids). Thus, if the dipeptides would be present randomly in proteins each amino acid dipeptide should be present with 0.25% frequency. As it is not the case in the nature, for better visablity all more than expected dipepetides are marked by red, and those which are underrepresented are marked by blue in the table.

All values are presented as per milles (‰), therefore need to be multiplied by 10^-3.

For more information see sequence space article on Wikipedia.

	Ala	Cys	Asp	Glu	Phe	Gly	His	Ile	Lys	Leu	Met	Asn	Pro	Gln	Arg	Ser	Thr	Val	Trp	Tyr	Xaa
Ala	5.684AlaAla: 5.684 ± 0.132	0.399AlaCys: 0.399 ± 0.028	4.125AlaAsp: 4.125 ± 0.093	4.818AlaGlu: 4.818 ± 0.104	3.297AlaPhe: 3.297 ± 0.071	5.456AlaGly: 5.456 ± 0.111	1.263AlaHis: 1.263 ± 0.048	5.806AlaIle: 5.806 ± 0.112	5.506AlaLys: 5.506 ± 0.093	7.549AlaLeu: 7.549 ± 0.112	1.868AlaMet: 1.868 ± 0.064	3.356AlaAsn: 3.356 ± 0.081	2.064AlaPro: 2.064 ± 0.06	3.16AlaGln: 3.16 ± 0.074	2.643AlaArg: 2.643 ± 0.075	4.512AlaSer: 4.512 ± 0.095	4.338AlaThr: 4.338 ± 0.102	4.863AlaVal: 4.863 ± 0.098	0.749AlaTrp: 0.749 ± 0.037	2.501AlaTyr: 2.501 ± 0.068	0.0AlaXaa: 0.0 ± 0.0
Cys	0.327CysAla: 0.327 ± 0.023	0.05CysCys: 0.05 ± 0.01	0.247CysAsp: 0.247 ± 0.021	0.286CysGlu: 0.286 ± 0.023	0.241CysPhe: 0.241 ± 0.02	0.438CysGly: 0.438 ± 0.028	0.114CysHis: 0.114 ± 0.014	0.262CysIle: 0.262 ± 0.02	0.202CysLys: 0.202 ± 0.018	0.484CysLeu: 0.484 ± 0.024	0.085CysMet: 0.085 ± 0.013	0.181CysAsn: 0.181 ± 0.017	0.233CysPro: 0.233 ± 0.02	0.19CysGln: 0.19 ± 0.018	0.181CysArg: 0.181 ± 0.018	0.344CysSer: 0.344 ± 0.023	0.204CysThr: 0.204 ± 0.022	0.227CysVal: 0.227 ± 0.021	0.041CysTrp: 0.041 ± 0.008	0.189CysTyr: 0.189 ± 0.017	0.0CysXaa: 0.0 ± 0.0
Asp	3.195AspAla: 3.195 ± 0.071	0.3AspCys: 0.3 ± 0.023	2.729AspAsp: 2.729 ± 0.073	4.58AspGlu: 4.58 ± 0.105	3.341AspPhe: 3.341 ± 0.09	3.592AspGly: 3.592 ± 0.103	0.851AspHis: 0.851 ± 0.035	3.793AspIle: 3.793 ± 0.078	4.349AspLys: 4.349 ± 0.094	5.53AspLeu: 5.53 ± 0.11	1.203AspMet: 1.203 ± 0.042	2.51AspAsn: 2.51 ± 0.064	1.47AspPro: 1.47 ± 0.051	1.615AspGln: 1.615 ± 0.05	1.752AspArg: 1.752 ± 0.058	3.379AspSer: 3.379 ± 0.093	2.369AspThr: 2.369 ± 0.069	3.267AspVal: 3.267 ± 0.071	0.7AspTrp: 0.7 ± 0.04	2.623AspTyr: 2.623 ± 0.067	0.0AspXaa: 0.0 ± 0.0
Glu	5.022GluAla: 5.022 ± 0.103	0.234GluCys: 0.234 ± 0.02	3.006GluAsp: 3.006 ± 0.073	5.469GluGlu: 5.469 ± 0.116	2.958GluPhe: 2.958 ± 0.079	3.448GluGly: 3.448 ± 0.092	1.224GluHis: 1.224 ± 0.047	6.416GluIle: 6.416 ± 0.117	6.678GluLys: 6.678 ± 0.117	7.276GluLeu: 7.276 ± 0.129	1.944GluMet: 1.944 ± 0.053	4.824GluAsn: 4.824 ± 0.103	1.56GluPro: 1.56 ± 0.057	2.674GluGln: 2.674 ± 0.065	2.813GluArg: 2.813 ± 0.086	3.606GluSer: 3.606 ± 0.085	3.519GluThr: 3.519 ± 0.077	4.708GluVal: 4.708 ± 0.098	0.533GluTrp: 0.533 ± 0.028	1.884GluTyr: 1.884 ± 0.063	0.0GluXaa: 0.0 ± 0.0
Phe	3.577PheAla: 3.577 ± 0.085	0.219PheCys: 0.219 ± 0.019	3.11PheAsp: 3.11 ± 0.08	3.331PheGlu: 3.331 ± 0.091	2.192PhePhe: 2.192 ± 0.079	3.533PheGly: 3.533 ± 0.068	0.787PheHis: 0.787 ± 0.034	3.758PheIle: 3.758 ± 0.097	2.831PheLys: 2.831 ± 0.07	4.557PheLeu: 4.557 ± 0.12	1.201PheMet: 1.201 ± 0.052	2.311PheAsn: 2.311 ± 0.056	1.638PhePro: 1.638 ± 0.05	1.381PheGln: 1.381 ± 0.043	1.437PheArg: 1.437 ± 0.05	3.5PheSer: 3.5 ± 0.09	2.76PheThr: 2.76 ± 0.07	3.37PheVal: 3.37 ± 0.075	0.543PheTrp: 0.543 ± 0.027	1.802PheTyr: 1.802 ± 0.054	0.0PheXaa: 0.0 ± 0.0
Gly	4.86GlyAla: 4.86 ± 0.12	0.32GlyCys: 0.32 ± 0.022	3.072GlyAsp: 3.072 ± 0.069	3.947GlyGlu: 3.947 ± 0.086	3.51GlyPhe: 3.51 ± 0.08	4.53GlyGly: 4.53 ± 0.12	1.34GlyHis: 1.34 ± 0.047	5.498GlyIle: 5.498 ± 0.094	4.908GlyLys: 4.908 ± 0.092	6.702GlyLeu: 6.702 ± 0.097	1.79GlyMet: 1.79 ± 0.061	3.139GlyAsn: 3.139 ± 0.08	1.376GlyPro: 1.376 ± 0.049	2.784GlyGln: 2.784 ± 0.066	2.423GlyArg: 2.423 ± 0.063	4.09GlySer: 4.09 ± 0.096	4.054GlyThr: 4.054 ± 0.122	4.481GlyVal: 4.481 ± 0.094	0.782GlyTrp: 0.782 ± 0.038	2.606GlyTyr: 2.606 ± 0.065	0.0GlyXaa: 0.0 ± 0.0
His	1.11HisAla: 1.11 ± 0.04	0.125HisCys: 0.125 ± 0.014	0.956HisAsp: 0.956 ± 0.04	1.231HisGlu: 1.231 ± 0.057	1.059HisPhe: 1.059 ± 0.04	1.285HisGly: 1.285 ± 0.044	0.454HisHis: 0.454 ± 0.025	1.163HisIle: 1.163 ± 0.044	0.929HisLys: 0.929 ± 0.039	1.915HisLeu: 1.915 ± 0.049	0.359HisMet: 0.359 ± 0.026	0.758HisAsn: 0.758 ± 0.036	0.862HisPro: 0.862 ± 0.038	0.868HisGln: 0.868 ± 0.034	0.764HisArg: 0.764 ± 0.036	0.98HisSer: 0.98 ± 0.039	0.836HisThr: 0.836 ± 0.037	1.052HisVal: 1.052 ± 0.039	0.195HisTrp: 0.195 ± 0.017	0.824HisTyr: 0.824 ± 0.037	0.0HisXaa: 0.0 ± 0.0
Ile	6.27IleAla: 6.27 ± 0.116	0.475IleCys: 0.475 ± 0.029	4.417IleAsp: 4.417 ± 0.087	5.011IleGlu: 5.011 ± 0.11	3.991IlePhe: 3.991 ± 0.096	5.218IleGly: 5.218 ± 0.101	1.286IleHis: 1.286 ± 0.054	6.004IleIle: 6.004 ± 0.13	5.048IleLys: 5.048 ± 0.096	7.479IleLeu: 7.479 ± 0.138	1.717IleMet: 1.717 ± 0.054	3.911IleAsn: 3.911 ± 0.079	2.996IlePro: 2.996 ± 0.073	2.355IleGln: 2.355 ± 0.059	2.502IleArg: 2.502 ± 0.064	5.675IleSer: 5.675 ± 0.102	4.35IleThr: 4.35 ± 0.089	5.259IleVal: 5.259 ± 0.104	0.725IleTrp: 0.725 ± 0.038	2.566IleTyr: 2.566 ± 0.073	0.0IleXaa: 0.0 ± 0.0
Lys	5.684LysAla: 5.684 ± 0.098	0.198LysCys: 0.198 ± 0.019	3.627LysAsp: 3.627 ± 0.097	6.002LysGlu: 6.002 ± 0.113	2.895LysPhe: 2.895 ± 0.068	3.745LysGly: 3.745 ± 0.086	1.123LysHis: 1.123 ± 0.038	6.265LysIle: 6.265 ± 0.103	6.465LysLys: 6.465 ± 0.113	6.404LysLeu: 6.404 ± 0.1	2.519LysMet: 2.519 ± 0.06	4.871LysAsn: 4.871 ± 0.104	1.86LysPro: 1.86 ± 0.051	2.337LysGln: 2.337 ± 0.07	2.892LysArg: 2.892 ± 0.072	4.344LysSer: 4.344 ± 0.092	4.169LysThr: 4.169 ± 0.089	5.073LysVal: 5.073 ± 0.1	0.653LysTrp: 0.653 ± 0.032	2.553LysTyr: 2.553 ± 0.07	0.0LysXaa: 0.0 ± 0.0
Leu	8.112LeuAla: 8.112 ± 0.125	0.423LeuCys: 0.423 ± 0.028	5.063LeuAsp: 5.063 ± 0.09	6.743LeuGlu: 6.743 ± 0.139	4.512LeuPhe: 4.512 ± 0.107	6.472LeuGly: 6.472 ± 0.112	1.522LeuHis: 1.522 ± 0.052	7.625LeuIle: 7.625 ± 0.148	6.924LeuLys: 6.924 ± 0.136	9.742LeuLeu: 9.742 ± 0.189	2.472LeuMet: 2.472 ± 0.061	4.81LeuAsn: 4.81 ± 0.078	3.83LeuPro: 3.83 ± 0.083	3.102LeuGln: 3.102 ± 0.062	3.399LeuArg: 3.399 ± 0.073	7.784LeuSer: 7.784 ± 0.121	6.474LeuThr: 6.474 ± 0.104	6.314LeuVal: 6.314 ± 0.122	0.822LeuTrp: 0.822 ± 0.04	2.815LeuTyr: 2.815 ± 0.066	0.0LeuXaa: 0.0 ± 0.0
Met	1.994MetAla: 1.994 ± 0.057	0.105MetCys: 0.105 ± 0.012	1.294MetAsp: 1.294 ± 0.044	1.442MetGlu: 1.442 ± 0.049	0.921MetPhe: 0.921 ± 0.037	1.554MetGly: 1.554 ± 0.052	0.414MetHis: 0.414 ± 0.023	2.13MetIle: 2.13 ± 0.065	2.181MetLys: 2.181 ± 0.061	2.133MetLeu: 2.133 ± 0.06	0.793MetMet: 0.793 ± 0.038	1.51MetAsn: 1.51 ± 0.053	0.88MetPro: 0.88 ± 0.036	0.837MetGln: 0.837 ± 0.037	0.971MetArg: 0.971 ± 0.044	1.688MetSer: 1.688 ± 0.055	2.098MetThr: 2.098 ± 0.054	1.56MetVal: 1.56 ± 0.052	0.184MetTrp: 0.184 ± 0.016	0.557MetTyr: 0.557 ± 0.031	0.0MetXaa: 0.0 ± 0.0
Asn	3.359AsnAla: 3.359 ± 0.078	0.248AsnCys: 0.248 ± 0.02	2.723AsnAsp: 2.723 ± 0.063	3.242AsnGlu: 3.242 ± 0.083	2.811AsnPhe: 2.811 ± 0.066	3.694AsnGly: 3.694 ± 0.096	1.139AsnHis: 1.139 ± 0.044	3.755AsnIle: 3.755 ± 0.068	3.326AsnLys: 3.326 ± 0.08	5.273AsnLeu: 5.273 ± 0.095	1.259AsnMet: 1.259 ± 0.045	2.472AsnAsn: 2.472 ± 0.073	2.548AsnPro: 2.548 ± 0.064	2.548AsnGln: 2.548 ± 0.073	1.907AsnArg: 1.907 ± 0.062	3.359AsnSer: 3.359 ± 0.125	2.484AsnThr: 2.484 ± 0.071	3.137AsnVal: 3.137 ± 0.074	0.644AsnTrp: 0.644 ± 0.03	2.07AsnTyr: 2.07 ± 0.065	0.0AsnXaa: 0.0 ± 0.0
Pro	2.254ProAla: 2.254 ± 0.062	0.11ProCys: 0.11 ± 0.014	1.819ProAsp: 1.819 ± 0.047	2.913ProGlu: 2.913 ± 0.081	1.652ProPhe: 1.652 ± 0.057	1.819ProGly: 1.819 ± 0.075	0.629ProHis: 0.629 ± 0.033	2.378ProIle: 2.378 ± 0.058	2.387ProLys: 2.387 ± 0.062	3.105ProLeu: 3.105 ± 0.068	0.779ProMet: 0.779 ± 0.036	1.766ProAsn: 1.766 ± 0.057	0.562ProPro: 0.562 ± 0.032	1.224ProGln: 1.224 ± 0.043	0.989ProArg: 0.989 ± 0.036	2.017ProSer: 2.017 ± 0.058	2.122ProThr: 2.122 ± 0.057	2.285ProVal: 2.285 ± 0.063	0.327ProTrp: 0.327 ± 0.027	1.204ProTyr: 1.204 ± 0.043	0.0ProXaa: 0.0 ± 0.0
Gln	3.003GlnAla: 3.003 ± 0.076	0.088GlnCys: 0.088 ± 0.011	1.542GlnAsp: 1.542 ± 0.048	2.877GlnGlu: 2.877 ± 0.071	1.547GlnPhe: 1.547 ± 0.05	2.13GlnGly: 2.13 ± 0.062	0.583GlnHis: 0.583 ± 0.032	3.022GlnIle: 3.022 ± 0.078	3.335GlnLys: 3.335 ± 0.075	3.723GlnLeu: 3.723 ± 0.09	1.034GlnMet: 1.034 ± 0.039	2.084GlnAsn: 2.084 ± 0.062	1.093GlnPro: 1.093 ± 0.042	1.298GlnGln: 1.298 ± 0.05	1.227GlnArg: 1.227 ± 0.049	2.099GlnSer: 2.099 ± 0.062	2.087GlnThr: 2.087 ± 0.055	2.571GlnVal: 2.571 ± 0.066	0.33GlnTrp: 0.33 ± 0.023	1.168GlnTyr: 1.168 ± 0.043	0.0GlnXaa: 0.0 ± 0.0
Arg	2.498ArgAla: 2.498 ± 0.068	0.149ArgCys: 0.149 ± 0.017	1.878ArgAsp: 1.878 ± 0.066	2.758ArgGlu: 2.758 ± 0.07	1.717ArgPhe: 1.717 ± 0.05	2.189ArgGly: 2.189 ± 0.068	0.7ArgHis: 0.7 ± 0.033	2.544ArgIle: 2.544 ± 0.057	2.913ArgLys: 2.913 ± 0.074	3.652ArgLeu: 3.652 ± 0.089	0.977ArgMet: 0.977 ± 0.044	1.709ArgAsn: 1.709 ± 0.055	1.196ArgPro: 1.196 ± 0.038	1.477ArgGln: 1.477 ± 0.049	1.767ArgArg: 1.767 ± 0.062	1.856ArgSer: 1.856 ± 0.051	1.874ArgThr: 1.874 ± 0.056	2.295ArgVal: 2.295 ± 0.068	0.344ArgTrp: 0.344 ± 0.023	1.34ArgTyr: 1.34 ± 0.05	0.0ArgXaa: 0.0 ± 0.0
Ser	4.522SerAla: 4.522 ± 0.103	0.277SerCys: 0.277 ± 0.022	3.562SerAsp: 3.562 ± 0.073	4.404SerGlu: 4.404 ± 0.105	3.143SerPhe: 3.143 ± 0.059	5.157SerGly: 5.157 ± 0.097	1.175SerHis: 1.175 ± 0.044	4.498SerIle: 4.498 ± 0.095	4.548SerLys: 4.548 ± 0.092	6.66SerLeu: 6.66 ± 0.127	1.419SerMet: 1.419 ± 0.051	3.23SerAsn: 3.23 ± 0.111	2.088SerPro: 2.088 ± 0.061	2.798SerGln: 2.798 ± 0.073	2.224SerArg: 2.224 ± 0.065	5.27SerSer: 5.27 ± 0.31	3.755SerThr: 3.755 ± 0.1	4.366SerVal: 4.366 ± 0.112	0.718SerTrp: 0.718 ± 0.038	2.265SerTyr: 2.265 ± 0.068	0.0SerXaa: 0.0 ± 0.0
Thr	4.195ThrAla: 4.195 ± 0.091	0.239ThrCys: 0.239 ± 0.021	3.668ThrAsp: 3.668 ± 0.071	3.58ThrGlu: 3.58 ± 0.074	2.735ThrPhe: 2.735 ± 0.071	4.3ThrGly: 4.3 ± 0.104	1.0ThrHis: 1.0 ± 0.038	4.271ThrIle: 4.271 ± 0.087	3.717ThrLys: 3.717 ± 0.078	5.407ThrLeu: 5.407 ± 0.095	1.187ThrMet: 1.187 ± 0.043	2.903ThrAsn: 2.903 ± 0.076	2.102ThrPro: 2.102 ± 0.06	1.878ThrGln: 1.878 ± 0.055	1.795ThrArg: 1.795 ± 0.056	3.967ThrSer: 3.967 ± 0.1	3.553ThrThr: 3.553 ± 0.116	4.058ThrVal: 4.058 ± 0.085	0.578ThrTrp: 0.578 ± 0.033	2.183ThrTyr: 2.183 ± 0.068	0.0ThrXaa: 0.0 ± 0.0
Val	5.381ValAla: 5.381 ± 0.11	0.335ValCys: 0.335 ± 0.028	3.623ValAsp: 3.623 ± 0.078	4.434ValGlu: 4.434 ± 0.105	2.813ValPhe: 2.813 ± 0.07	4.542ValGly: 4.542 ± 0.105	1.108ValHis: 1.108 ± 0.049	5.174ValIle: 5.174 ± 0.09	4.653ValLys: 4.653 ± 0.095	6.411ValLeu: 6.411 ± 0.12	1.636ValMet: 1.636 ± 0.05	3.337ValAsn: 3.337 ± 0.084	2.404ValPro: 2.404 ± 0.061	2.037ValGln: 2.037 ± 0.054	2.294ValArg: 2.294 ± 0.064	4.675ValSer: 4.675 ± 0.091	4.034ValThr: 4.034 ± 0.093	4.618ValVal: 4.618 ± 0.101	0.546ValTrp: 0.546 ± 0.03	2.091ValTyr: 2.091 ± 0.06	0.0ValXaa: 0.0 ± 0.0
Trp	0.607TrpAla: 0.607 ± 0.028	0.049TrpCys: 0.049 ± 0.008	0.531TrpAsp: 0.531 ± 0.027	0.524TrpGlu: 0.524 ± 0.031	0.524TrpPhe: 0.524 ± 0.028	0.665TrpGly: 0.665 ± 0.038	0.227TrpHis: 0.227 ± 0.02	0.674TrpIle: 0.674 ± 0.035	0.635TrpLys: 0.635 ± 0.033	1.187TrpLeu: 1.187 ± 0.049	0.272TrpMet: 0.272 ± 0.024	0.553TrpAsn: 0.553 ± 0.034	0.204TrpPro: 0.204 ± 0.02	0.495TrpGln: 0.495 ± 0.027	0.414TrpArg: 0.414 ± 0.03	0.679TrpSer: 0.679 ± 0.034	0.566TrpThr: 0.566 ± 0.033	0.645TrpVal: 0.645 ± 0.031	0.167TrpTrp: 0.167 ± 0.018	0.371TrpTyr: 0.371 ± 0.03	0.0TrpXaa: 0.0 ± 0.0
Tyr	2.3TyrAla: 2.3 ± 0.06	0.184TyrCys: 0.184 ± 0.018	2.155TyrAsp: 2.155 ± 0.058	2.204TyrGlu: 2.204 ± 0.061	1.979TyrPhe: 1.979 ± 0.055	2.445TyrGly: 2.445 ± 0.069	0.784TyrHis: 0.784 ± 0.035	2.221TyrIle: 2.221 ± 0.063	2.145TyrLys: 2.145 ± 0.06	3.772TyrLeu: 3.772 ± 0.088	0.729TyrMet: 0.729 ± 0.036	1.767TyrAsn: 1.767 ± 0.057	1.375TyrPro: 1.375 ± 0.046	1.782TyrGln: 1.782 ± 0.053	1.438TyrArg: 1.438 ± 0.055	2.256TyrSer: 2.256 ± 0.063	1.673TyrThr: 1.673 ± 0.065	1.996TyrVal: 1.996 ± 0.059	0.406TyrTrp: 0.406 ± 0.023	1.498TyrTyr: 1.498 ± 0.054	0.0TyrXaa: 0.0 ± 0.0
Xaa	0.0XaaAla: 0.0 ± 0.0	0.0XaaCys: 0.0 ± 0.0	0.0XaaAsp: 0.0 ± 0.0	0.0XaaGlu: 0.0 ± 0.0	0.0XaaPhe: 0.0 ± 0.0	0.0XaaGly: 0.0 ± 0.0	0.0XaaHis: 0.0 ± 0.0	0.0XaaIle: 0.0 ± 0.0	0.0XaaLys: 0.0 ± 0.0	0.0XaaLeu: 0.0 ± 0.0	0.0XaaMet: 0.0 ± 0.0	0.0XaaAsn: 0.0 ± 0.0	0.0XaaPro: 0.0 ± 0.0	0.0XaaGln: 0.0 ± 0.0	0.0XaaArg: 0.0 ± 0.0	0.0XaaSer: 0.0 ± 0.0	0.0XaaThr: 0.0 ± 0.0	0.0XaaVal: 0.0 ± 0.0	0.0XaaTrp: 0.0 ± 0.0	0.0XaaTyr: 0.0 ± 0.0	0.0XaaXaa: 0.0 ± 0.0

Statistics based on 2225 proteins (656950 amino acids)

Note: The error has been estimated with the bootstraping (x100) at the protein level

Above dipeptide statistics (among other stats for this proteome) you can download from this CSV file

Reference: Kozlowski LP. Proteome-pI 2.0: Proteome Isoelectric Point Database Update. Nucleic Acids Res. 2021, doi: 10.1093/nar/gkab944

Contact: Lukasz P. Kozlowski

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