Coprobacillus sp. CAG:235
Average proteome isoelectric point is 6.35
Get precalculated fractions of proteins
Acidic
pI < 6.8
6.8-7.4
pI > 7.4
Basic
All
Note: above files contain also dissociation constants (pKa)
Virtual 2D-PAGE plot for 2339 proteins (isoelectric point calculated using IPC2_protein)
Get csv file with sequences according to given criteria:
* You can choose from 21 different methods for calculating isoelectric point
Summary statistics related to proteome-wise predictions
Protein with the lowest isoelectric point:
>tr|R5Q5N3|R5Q5N3_9FIRM Uncharacterized protein OS=Coprobacillus sp. CAG:235 OX=1262854 GN=BN550_01655 PE=4 SV=1
MM1 pKa = 7.09 KK2 pKa = 10.23 VVYY5 pKa = 10.35 ASRR8 pKa = 11.84 TGNVEE13 pKa = 4.33 SIISKK18 pKa = 10.6 LGIEE22 pKa = 4.64 DD23 pKa = 3.67 ALKK26 pKa = 11.07 VEE28 pKa = 5.23 DD29 pKa = 3.98 GSEE32 pKa = 4.28 TVEE35 pKa = 3.49 EE36 pKa = 4.54 DD37 pKa = 3.96 YY38 pKa = 11.41 ILFTYY43 pKa = 10.26 TDD45 pKa = 3.48 GFGDD49 pKa = 3.37 VPYY52 pKa = 10.53 EE53 pKa = 3.78 VDD55 pKa = 4.89 EE56 pKa = 4.49 FLQSQSEE63 pKa = 4.24 HH64 pKa = 6.18 LKK66 pKa = 10.21 GVVVSGDD73 pKa = 3.2 TGYY76 pKa = 11.73 GEE78 pKa = 5.26 AYY80 pKa = 10.01 CKK82 pKa = 10.66 AGDD85 pKa = 4.37 VIAEE89 pKa = 4.21 QYY91 pKa = 10.64 NVPCLYY97 pKa = 10.43 KK98 pKa = 10.52 VEE100 pKa = 4.55 NDD102 pKa = 3.88 GSDD105 pKa = 3.55 EE106 pKa = 4.73 DD107 pKa = 3.76 IEE109 pKa = 4.76 AIKK112 pKa = 10.61 KK113 pKa = 9.76 VIAA116 pKa = 4.19
Molecular weight: 12.72 kDa
Isoelectric point according different methods:
IPC2.protein.svr19 3.821
IPC2_protein 4.05
IPC_protein 3.986
Toseland 3.808
ProMoST 4.113
Dawson 3.948
Bjellqvist 4.101
Wikipedia 3.846
Rodwell 3.821
Grimsley 3.719
Solomon 3.935
Lehninger 3.897
Nozaki 4.062
DTASelect 4.228
Thurlkill 3.834
EMBOSS 3.859
Sillero 4.101
Patrickios 1.914
IPC_peptide 3.948
IPC2_peptide 4.088
IPC2.peptide.svr19 3.988
Protein with the highest isoelectric point:
>tr|R5Q5J6|R5Q5J6_9FIRM Uncharacterized protein OS=Coprobacillus sp. CAG:235 OX=1262854 GN=BN550_00005 PE=3 SV=1
MM1 pKa = 7.35 KK2 pKa = 9.42 RR3 pKa = 11.84 TYY5 pKa = 10.34 QPNKK9 pKa = 8.62 RR10 pKa = 11.84 KK11 pKa = 9.56 RR12 pKa = 11.84 AKK14 pKa = 8.76 THH16 pKa = 5.23 GFRR19 pKa = 11.84 ARR21 pKa = 11.84 MATVGGRR28 pKa = 11.84 KK29 pKa = 9.34 VIARR33 pKa = 11.84 RR34 pKa = 11.84 RR35 pKa = 11.84 KK36 pKa = 8.97 RR37 pKa = 11.84 GRR39 pKa = 11.84 KK40 pKa = 9.13 VLTAA44 pKa = 4.32
Molecular weight: 5.19 kDa
Isoelectric point according different methods:
IPC2.protein.svr19 9.433
IPC2_protein 11.125
IPC_protein 12.501
Toseland 12.661
ProMoST 13.159
Dawson 12.661
Bjellqvist 12.661
Wikipedia 13.144
Rodwell 12.398
Grimsley 12.705
Solomon 13.159
Lehninger 13.056
Nozaki 12.661
DTASelect 12.661
Thurlkill 12.661
EMBOSS 13.159
Sillero 12.661
Patrickios 12.135
IPC_peptide 13.159
IPC2_peptide 12.149
IPC2.peptide.svr19 9.06
Peptides (in silico digests for buttom-up proteomics)
Below you can find
in silico digests of the whole proteome with Trypsin, Chymotrypsin, Trypsin+LysC, LysN, ArgC proteases suitable for different mass spec machines.
Try ESI
ChTry ESI
ArgC ESI
LysN ESI
TryLysC ESI
Try MALDI
ChTry MALDI
ArgC MALDI
LysN MALDI
TryLysC MALDI
Try LTQ
ChTry LTQ
ArgC LTQ
LysN LTQ
TryLysC LTQ
Try MSlow
ChTry MSlow
ArgC MSlow
LysN MSlow
TryLysC MSlow
Try MShigh
ChTry MShigh
ArgC MShigh
LysN MShigh
TryLysC MShigh
General Statistics
Number of major isoforms
Number of additional isoforms
Number of all proteins
Number of amino acids
Min. Seq. Length
Max. Seq. Length
Avg. Seq. Length
Avg. Mol. Weight
2339
0
2339
677759
32
2948
289.8
33.03
Amino acid frequency
Ala
Cys
Asp
Glu
Phe
Gly
His
Ile
Lys
Leu
5.4 ± 0.054
1.418 ± 0.017
6.013 ± 0.046
6.958 ± 0.055
4.42 ± 0.039
5.984 ± 0.056
2.173 ± 0.027
8.866 ± 0.056
8.538 ± 0.053
9.403 ± 0.059
Met
Asn
Gln
Pro
Arg
Ser
Thr
Val
Trp
Tyr
2.887 ± 0.026
5.591 ± 0.046
2.698 ± 0.025
3.723 ± 0.033
3.143 ± 0.036
5.71 ± 0.044
4.969 ± 0.046
6.483 ± 0.042
0.632 ± 0.018
4.991 ± 0.052
Note: For amino acid frequency statistics the error has been estimated with the bootstraping (x100) at the protein level
Most of the basic statistics you can see at this page can be downloaded from this CSV file
For dipeptide frequency statistics click here