Sanxia narna-like virus 1
Average proteome isoelectric point is 8.89
Get precalculated fractions of proteins
Acidic
pI < 6.8
6.8-7.4
pI > 7.4
Basic
All
Note: above files contain also dissociation constants (pKa)
Virtual 2D-PAGE plot for 2 proteins (isoelectric point calculated using IPC2_protein)
Get csv file with sequences according to given criteria:
* You can choose from 21 different methods for calculating isoelectric point
Summary statistics related to proteome-wise predictions
Protein with the lowest isoelectric point:
>tr|A0A1L3KIL2|A0A1L3KIL2_9VIRU RNA-dependent RNA polymerase OS=Sanxia narna-like virus 1 OX=1923360 PE=4 SV=1
MM1 pKa = 7.87 KK2 pKa = 10.2 YY3 pKa = 11.09 NLMTKK8 pKa = 10.1 SKK10 pKa = 9.14 TKK12 pKa = 10.66 SNQKK16 pKa = 8.64 PAQRR20 pKa = 11.84 AKK22 pKa = 10.31 PRR24 pKa = 11.84 RR25 pKa = 11.84 SRR27 pKa = 11.84 QRR29 pKa = 11.84 ANKK32 pKa = 8.91 VQDD35 pKa = 3.52 GLDD38 pKa = 3.88 GYY40 pKa = 10.9 GLAAARR46 pKa = 11.84 MYY48 pKa = 11.19 ADD50 pKa = 3.59 PCGADD55 pKa = 3.75 LVPTVYY61 pKa = 10.65 SGDD64 pKa = 3.18 RR65 pKa = 11.84 GYY67 pKa = 10.78 INRR70 pKa = 11.84 FVANASVAITAGDD83 pKa = 3.44 TSLILLIKK91 pKa = 10.01 PGNQTAFFRR100 pKa = 11.84 ADD102 pKa = 3.58 TAPTTPGAIGYY113 pKa = 8.69 DD114 pKa = 3.2 NSIFPGRR121 pKa = 11.84 SFYY124 pKa = 10.8 TSNASKK130 pKa = 10.47 ARR132 pKa = 11.84 CAGYY136 pKa = 10.11 CINVRR141 pKa = 11.84 PNSAPNNATGTIYY154 pKa = 10.6 FGNINASAVPNLASITASGLIPLLSEE180 pKa = 4.77 SVSCSQALMQPLEE193 pKa = 4.6 VKK195 pKa = 9.69 WSPGGFDD202 pKa = 4.8 DD203 pKa = 5.45 RR204 pKa = 11.84 YY205 pKa = 9.18 NTIGTITDD213 pKa = 3.78 DD214 pKa = 4.27 DD215 pKa = 4.35 SDD217 pKa = 5.02 RR218 pKa = 11.84 NVLCIVVLGLPAASGMQIRR237 pKa = 11.84 QTVITEE243 pKa = 4.18 WSPANALTVTNDD255 pKa = 2.79 ATAVKK260 pKa = 10.1 PSVCDD265 pKa = 3.29 KK266 pKa = 11.31 DD267 pKa = 4.55 CILRR271 pKa = 11.84 HH272 pKa = 6.14 LKK274 pKa = 10.59 RR275 pKa = 11.84 KK276 pKa = 10.25 DD277 pKa = 3.5 SAWWWKK283 pKa = 10.32 LGHH286 pKa = 6.57 KK287 pKa = 10.23 VLDD290 pKa = 3.67 VGKK293 pKa = 9.35 SVGMGYY299 pKa = 8.19 YY300 pKa = 9.81 TGGAVGALGAAVRR313 pKa = 11.84 FMM315 pKa = 4.85
Molecular weight: 33.66 kDa
Isoelectric point according different methods:
IPC2.protein.svr19 8.862
IPC2_protein 8.858
IPC_protein 8.785
Toseland 9.311
ProMoST 9.209
Dawson 9.648
Bjellqvist 9.472
Wikipedia 9.838
Rodwell 9.838
Grimsley 9.721
Solomon 9.692
Lehninger 9.648
Nozaki 9.575
DTASelect 9.399
Thurlkill 9.502
EMBOSS 9.78
Sillero 9.648
Patrickios 4.647
IPC_peptide 9.692
IPC2_peptide 8.346
IPC2.peptide.svr19 7.805
Protein with the highest isoelectric point:
>tr|A0A1L3KIL2|A0A1L3KIL2_9VIRU RNA-dependent RNA polymerase OS=Sanxia narna-like virus 1 OX=1923360 PE=4 SV=1
MM1 pKa = 6.93 TPQMRR6 pKa = 11.84 EE7 pKa = 3.93 HH8 pKa = 6.44 NSARR12 pKa = 11.84 SRR14 pKa = 11.84 APRR17 pKa = 11.84 MSKK20 pKa = 10.56 DD21 pKa = 3.05 MHH23 pKa = 6.6 LRR25 pKa = 11.84 SPLNRR30 pKa = 11.84 GPKK33 pKa = 10.07 GADD36 pKa = 2.98 FLVRR40 pKa = 11.84 IPEE43 pKa = 4.22 LVADD47 pKa = 4.59 PVQVSKK53 pKa = 11.23 LSAKK57 pKa = 9.69 GVWHH61 pKa = 6.86 KK62 pKa = 11.23 SDD64 pKa = 3.35 NALVEE69 pKa = 4.13 TFRR72 pKa = 11.84 LIGYY76 pKa = 7.09 EE77 pKa = 4.23 FKK79 pKa = 11.04 NVLCPSPPTSSVPEE93 pKa = 4.03 VMSVLKK99 pKa = 10.7 NWVAFWLPWFLGDD112 pKa = 4.06 DD113 pKa = 3.88 TPPQRR118 pKa = 11.84 FNPLSLCSPGFRR130 pKa = 11.84 SFIRR134 pKa = 11.84 NRR136 pKa = 11.84 KK137 pKa = 5.37 TSKK140 pKa = 8.77 TGKK143 pKa = 8.9 SRR145 pKa = 11.84 KK146 pKa = 9.08 IRR148 pKa = 11.84 IGALLLYY155 pKa = 9.99 SKK157 pKa = 10.74 RR158 pKa = 11.84 LFPSFTGEE166 pKa = 3.91 MVRR169 pKa = 11.84 EE170 pKa = 3.99 KK171 pKa = 10.98 VKK173 pKa = 10.82 EE174 pKa = 3.95 FGDD177 pKa = 3.49 AVTRR181 pKa = 11.84 VSPGDD186 pKa = 3.52 LPRR189 pKa = 11.84 KK190 pKa = 8.54 RR191 pKa = 11.84 KK192 pKa = 8.78 MYY194 pKa = 10.79 GSIEE198 pKa = 3.92 QSIDD202 pKa = 2.97 EE203 pKa = 4.29 FLQGGRR209 pKa = 11.84 MVADD213 pKa = 3.43 YY214 pKa = 10.28 SKK216 pKa = 10.59 PFAPSTSACYY226 pKa = 9.63 EE227 pKa = 3.77 KK228 pKa = 10.87 SRR230 pKa = 11.84 AEE232 pKa = 4.01 GGLQRR237 pKa = 11.84 FVADD241 pKa = 4.45 NILQEE246 pKa = 4.43 TVEE249 pKa = 4.36 SRR251 pKa = 11.84 FFYY254 pKa = 10.82 DD255 pKa = 4.68 DD256 pKa = 4.91 LNHH259 pKa = 6.37 IWKK262 pKa = 10.32 CSEE265 pKa = 4.31 DD266 pKa = 4.05 DD267 pKa = 3.79 LTFGTPIGLWNEE279 pKa = 3.96 FLDD282 pKa = 3.73 IMFRR286 pKa = 11.84 RR287 pKa = 11.84 AMSEE291 pKa = 3.59 TGLPDD296 pKa = 3.69 QEE298 pKa = 4.43 WKK300 pKa = 10.5 RR301 pKa = 11.84 LPVQATGLTEE311 pKa = 3.93 PLKK314 pKa = 11.01 VRR316 pKa = 11.84 IVTKK320 pKa = 10.42 SNWFLQLLTPIQKK333 pKa = 9.54 AWHH336 pKa = 5.81 GAMRR340 pKa = 11.84 SHH342 pKa = 8.26 PIYY345 pKa = 10.45 QLIGGIPVEE354 pKa = 4.11 QALLGLDD361 pKa = 3.79 LQRR364 pKa = 11.84 KK365 pKa = 7.08 QKK367 pKa = 10.27 VVSGDD372 pKa = 3.3 YY373 pKa = 10.71 SAATDD378 pKa = 4.13 NIFLEE383 pKa = 4.38 YY384 pKa = 10.23 TEE386 pKa = 4.53 YY387 pKa = 10.87 AAKK390 pKa = 10.82 AMLEE394 pKa = 3.97 RR395 pKa = 11.84 TDD397 pKa = 5.67 FSFLDD402 pKa = 3.68 PQLVQFVPWIKK413 pKa = 10.64 KK414 pKa = 9.71 LVIHH418 pKa = 5.79 SLVRR422 pKa = 11.84 SSLEE426 pKa = 3.94 LKK428 pKa = 10.72 GMEE431 pKa = 4.66 PKK433 pKa = 10.65 DD434 pKa = 3.27 ITRR437 pKa = 11.84 GQMMGHH443 pKa = 6.87 ILSFPLLCLINRR455 pKa = 11.84 SASVMAIPRR464 pKa = 11.84 EE465 pKa = 3.56 RR466 pKa = 11.84 FMRR469 pKa = 11.84 INGDD473 pKa = 3.51 DD474 pKa = 3.6 VLFPANPTEE483 pKa = 3.79 YY484 pKa = 10.17 RR485 pKa = 11.84 KK486 pKa = 9.42 WKK488 pKa = 10.05 RR489 pKa = 11.84 ATASVGLEE497 pKa = 3.59 FSLGKK502 pKa = 10.21 NYY504 pKa = 10.31 YY505 pKa = 10.29 SRR507 pKa = 11.84 DD508 pKa = 3.39 LALVNSTYY516 pKa = 10.73 CVWDD520 pKa = 3.68 KK521 pKa = 11.68 ASARR525 pKa = 11.84 WKK527 pKa = 10.13 VLSVPNVGLLNMPMEE542 pKa = 4.34 KK543 pKa = 10.19 QIDD546 pKa = 4.16 TEE548 pKa = 4.19 TGRR551 pKa = 11.84 QIMPWEE557 pKa = 3.96 VLAQNWRR564 pKa = 11.84 EE565 pKa = 3.76 FSRR568 pKa = 11.84 FSTKK572 pKa = 8.66 KK573 pKa = 7.22 TQSTFVRR580 pKa = 11.84 LFLKK584 pKa = 10.18 HH585 pKa = 5.77 YY586 pKa = 8.58 PILRR590 pKa = 11.84 GFPGPIFGPTQWGGLGAPIPDD611 pKa = 2.98 GHH613 pKa = 6.73 KK614 pKa = 9.4 FTRR617 pKa = 11.84 NQLMWMNAHH626 pKa = 6.77 RR627 pKa = 11.84 LGMFSYY633 pKa = 10.74 LQGTRR638 pKa = 11.84 TDD640 pKa = 3.55 YY641 pKa = 11.45 SRR643 pKa = 11.84 LSEE646 pKa = 4.35 MYY648 pKa = 9.2 AQQIEE653 pKa = 4.66 KK654 pKa = 9.82 YY655 pKa = 8.13 VTGIYY660 pKa = 8.88 EE661 pKa = 3.94 WRR663 pKa = 11.84 EE664 pKa = 3.69 PEE666 pKa = 4.13 YY667 pKa = 11.11 GDD669 pKa = 3.93 SFGPLEE675 pKa = 4.8 SGVFMDD681 pKa = 5.75 PYY683 pKa = 11.05 SKK685 pKa = 10.92 DD686 pKa = 2.7 GGYY689 pKa = 10.56 AGQVMALRR697 pKa = 11.84 RR698 pKa = 11.84 WVVDD702 pKa = 3.82 ASSMKK707 pKa = 10.05 HH708 pKa = 5.02 IKK710 pKa = 10.14 IFGARR715 pKa = 11.84 RR716 pKa = 11.84 WNQYY720 pKa = 10.18 KK721 pKa = 10.72 LSLKK725 pKa = 9.87 TGIPPLPQFYY735 pKa = 10.46 LDD737 pKa = 4.09 KK738 pKa = 10.92 LRR740 pKa = 11.84 SGDD743 pKa = 3.15 IYY745 pKa = 10.81 FPRR748 pKa = 11.84 PGWNRR753 pKa = 11.84 SRR755 pKa = 11.84 DD756 pKa = 3.78 MTGSRR761 pKa = 11.84 YY762 pKa = 8.91 EE763 pKa = 3.98 DD764 pKa = 3.45 RR765 pKa = 11.84 ATYY768 pKa = 10.01 LHH770 pKa = 7.07 EE771 pKa = 4.81 IFPSTLKK778 pKa = 10.91 NN779 pKa = 3.34
Molecular weight: 89.53 kDa
Isoelectric point according different methods:
IPC2.protein.svr19 8.924
IPC2_protein 9.077
IPC_protein 9.019
Toseland 9.706
ProMoST 9.458
Dawson 9.97
Bjellqvist 9.663
Wikipedia 10.145
Rodwell 10.262
Grimsley 10.043
Solomon 9.984
Lehninger 9.94
Nozaki 9.736
DTASelect 9.648
Thurlkill 9.794
EMBOSS 10.131
Sillero 9.882
Patrickios 5.474
IPC_peptide 9.97
IPC2_peptide 8.244
IPC2.peptide.svr19 8.092
Peptides (in silico digests for buttom-up proteomics)
Below you can find
in silico digests of the whole proteome with Trypsin, Chymotrypsin, Trypsin+LysC, LysN, ArgC proteases suitable for different mass spec machines.
Try ESI
ChTry ESI
ArgC ESI
LysN ESI
TryLysC ESI
Try MALDI
ChTry MALDI
ArgC MALDI
LysN MALDI
TryLysC MALDI
Try LTQ
ChTry LTQ
ArgC LTQ
LysN LTQ
TryLysC LTQ
Try MSlow
ChTry MSlow
ArgC MSlow
LysN MSlow
TryLysC MSlow
Try MShigh
ChTry MShigh
ArgC MShigh
LysN MShigh
TryLysC MShigh
General Statistics
Number of major isoforms
Number of additional isoforms
Number of all proteins
Number of amino acids
Min. Seq. Length
Max. Seq. Length
Avg. Seq. Length
Avg. Mol. Weight
2
0
2
1094
315
779
547.0
61.6
Amino acid frequency
Ala
Cys
Asp
Glu
Phe
Gly
His
Ile
Lys
Leu
7.13 ± 2.309
1.188 ± 0.517
5.21 ± 0.411
3.931 ± 1.49
4.57 ± 1.016
7.495 ± 0.697
1.371 ± 0.368
4.845 ± 0.276
5.941 ± 0.272
8.867 ± 0.942
Met
Asn
Gln
Pro
Arg
Ser
Thr
Val
Trp
Tyr
3.199 ± 0.489
4.022 ± 1.005
6.307 ± 0.455
3.565 ± 0.354
7.13 ± 0.708
7.861 ± 0.038
5.484 ± 0.75
5.85 ± 0.409
2.377 ± 0.395
3.656 ± 0.077
Note: For amino acid frequency statistics the error has been estimated with the bootstraping (x100) at the protein level
Most of the basic statistics you can see at this page can be downloaded from this CSV file
For dipeptide frequency statistics click here