Bromus-associated circular DNA virus 1

Taxonomy: Viruses; unclassified viruses; unclassified DNA viruses; unclassified ssDNA viruses

Average proteome isoelectric point is 7.31

Get precalculated fractions of proteins

Acidic
pI < 6.8
6.8-7.4
pI > 7.4
Basic
    
All



Virtual 2D-PAGE plot for 4 proteins (isoelectric point calculated using IPC2_protein)

Get csv file with sequences according to given criteria:
        -     Method 

     -  kDa    
                                                                                      

* You can choose from 21 different methods for calculating isoelectric point

Summary statistics related to proteome-wise predictions


    
Protein with the lowest isoelectric point:
>tr|A0A0B4U9K0|A0A0B4U9K0_9VIRU Uncharacterized protein OS=Bromus-associated circular DNA virus 1 OX=1590154 PE=4 SV=1
MM1 pKa = 7.82ASGLPGEE8 pKa = 4.48HH9 pKa = 6.62SGDD12 pKa = 3.3GGEE15 pKa = 3.97RR16 pKa = 11.84SVVARR21 pKa = 11.84HH22 pKa = 5.63VPDD25 pKa = 3.69EE26 pKa = 4.23QVCGQSPEE34 pKa = 4.12IRR36 pKa = 11.84SGDD39 pKa = 3.44QKK41 pKa = 11.28LRR43 pKa = 11.84GAGRR47 pKa = 11.84YY48 pKa = 8.85LDD50 pKa = 4.06YY51 pKa = 11.11IRR53 pKa = 11.84LCFAAWCCSEE63 pKa = 4.62HH64 pKa = 6.17PQSGFCEE71 pKa = 4.1PVYY74 pKa = 10.93QPDD77 pKa = 2.8QRR79 pKa = 11.84LAYY82 pKa = 9.63RR83 pKa = 11.84PSEE86 pKa = 4.04RR87 pKa = 11.84VQSGGACGLPEE98 pKa = 4.7SDD100 pKa = 3.47VRR102 pKa = 11.84FAGYY106 pKa = 10.08HH107 pKa = 6.14PLSQYY112 pKa = 11.89ALLQTVQDD120 pKa = 4.01QEE122 pKa = 4.28DD123 pKa = 4.11HH124 pKa = 6.18QGHH127 pKa = 7.4PGTGSGASPPCYY139 pKa = 9.59RR140 pKa = 11.84EE141 pKa = 3.74AAEE144 pKa = 4.45HH145 pKa = 5.8VRR147 pKa = 11.84HH148 pKa = 6.27SDD150 pKa = 3.0VGQCEE155 pKa = 4.04FCAVYY160 pKa = 10.17GGPGLPYY167 pKa = 10.46PGDD170 pKa = 3.16FWVYY174 pKa = 8.97PDD176 pKa = 4.22YY177 pKa = 11.36GAGQHH182 pKa = 5.25WW183 pKa = 3.56

Molecular weight: 19.96 kDa
Isoelectric point according different methods:





Protein with the highest isoelectric point:
>tr|A0A0B4U8V1|A0A0B4U8V1_9VIRU Replication-associated protein OS=Bromus-associated circular DNA virus 1 OX=1590154 PE=3 SV=1
MM1 pKa = 7.65RR2 pKa = 11.84AFAFRR7 pKa = 11.84GAATTLGFIADD18 pKa = 4.15NLRR21 pKa = 11.84GAHH24 pKa = 5.05TAYY27 pKa = 10.84RR28 pKa = 11.84MAKK31 pKa = 7.16GTSYY35 pKa = 10.83GRR37 pKa = 11.84SRR39 pKa = 11.84RR40 pKa = 11.84IPARR44 pKa = 11.84NAKK47 pKa = 9.98SLGIGSRR54 pKa = 11.84SKK56 pKa = 9.96RR57 pKa = 11.84TAAVSKK63 pKa = 9.15FRR65 pKa = 11.84RR66 pKa = 11.84VKK68 pKa = 10.42RR69 pKa = 11.84VVVKK73 pKa = 9.38PKK75 pKa = 10.02RR76 pKa = 11.84KK77 pKa = 9.91SFTRR81 pKa = 11.84TKK83 pKa = 10.68RR84 pKa = 11.84RR85 pKa = 11.84TSYY88 pKa = 10.18KK89 pKa = 9.65AGRR92 pKa = 11.84GGFSSALAKK101 pKa = 10.14TGGSSFSIGSGAKK114 pKa = 10.12LYY116 pKa = 10.84RR117 pKa = 11.84GFKK120 pKa = 8.93WAQQPVTFTSTTTQRR135 pKa = 11.84VTGAAGKK142 pKa = 9.59QAVAVLPTYY151 pKa = 10.49RR152 pKa = 11.84INPGANDD159 pKa = 3.85PTNDD163 pKa = 3.26TALIGTNTLDD173 pKa = 4.07SVLLDD178 pKa = 3.49RR179 pKa = 11.84AEE181 pKa = 4.52RR182 pKa = 11.84WLQAYY187 pKa = 8.88QEE189 pKa = 4.5STPATAASAQSSPGMYY205 pKa = 8.28QTNKK209 pKa = 9.91FVVNHH214 pKa = 6.49LKK216 pKa = 10.36YY217 pKa = 10.81DD218 pKa = 3.53QAIRR222 pKa = 11.84NCEE225 pKa = 4.07VQDD228 pKa = 3.74VTLTIYY234 pKa = 10.95DD235 pKa = 3.71CVLRR239 pKa = 11.84PGVAPNIRR247 pKa = 11.84SPGSVNQSISPINDD261 pKa = 2.74WHH263 pKa = 7.34IGLQNEE269 pKa = 4.51YY270 pKa = 10.91NPAAPVAYY278 pKa = 10.39LSQTFDD284 pKa = 3.57SPATTPFHH292 pKa = 6.09STHH295 pKa = 5.64FCKK298 pKa = 10.47LYY300 pKa = 10.47KK301 pKa = 9.83IKK303 pKa = 9.79KK304 pKa = 4.37TTKK307 pKa = 8.45VTLAPGAVHH316 pKa = 6.25HH317 pKa = 6.25HH318 pKa = 6.35HH319 pKa = 6.14VTVKK323 pKa = 8.88PRR325 pKa = 11.84NMFDD329 pKa = 3.19TQTSGNVNSALYY341 pKa = 9.04TADD344 pKa = 3.57RR345 pKa = 11.84GYY347 pKa = 10.72LIPGISGFTLITVLGSIGDD366 pKa = 3.79SAANHH371 pKa = 6.08LLIGTTKK378 pKa = 9.5TAVDD382 pKa = 3.86VVTTTMASFSQFSRR396 pKa = 11.84EE397 pKa = 3.95RR398 pKa = 11.84RR399 pKa = 11.84MHH401 pKa = 5.62MAFDD405 pKa = 4.55GLTQATDD412 pKa = 3.79LQAVQPDD419 pKa = 3.69TGTIQMDD426 pKa = 3.82TTANEE431 pKa = 3.83AA432 pKa = 3.47

Molecular weight: 46.82 kDa
Isoelectric point according different methods:





Peptides (in silico digests for buttom-up proteomics)

Below you can find in silico digests of the whole proteome with Trypsin, Chymotrypsin, Trypsin+LysC, LysN, ArgC proteases suitable for different mass spec machines.

Try
ESI
ChTry
ESI
ArgC
ESI
LysN
ESI
TryLysC
ESI

Try
MALDI
ChTry
MALDI
ArgC
MALDI
LysN
MALDI
TryLysC
MALDI

Try
LTQ
ChTry
LTQ
ArgC
LTQ
LysN
LTQ
TryLysC
LTQ

Try
MSlow
ChTry
MSlow
ArgC
MSlow
LysN
MSlow
TryLysC
MSlow

Try
MShigh
ChTry
MShigh
ArgC
MShigh
LysN
MShigh
TryLysC
MShigh

General Statistics

Number of major isoforms

Number of additional isoforms

Number of all proteins

Number of amino acids

Min. Seq. Length

Max. Seq. Length

Avg. Seq. Length

Avg. Mol. Weight

4

0

4

1180

183

432

295.0

32.6

Amino acid frequency

Ala

Cys

Asp

Glu

Phe

Gly

His

Ile

Lys

Leu

8.644 ± 1.149

2.119 ± 0.66

5.763 ± 0.836

3.39 ± 0.914

4.237 ± 0.93

8.305 ± 1.282

2.966 ± 0.4

4.322 ± 0.885

3.898 ± 0.82

7.712 ± 1.167

Met

Asn

Gln

Pro

Arg

Ser

Thr

Val

Trp

Tyr

1.271 ± 0.248

3.305 ± 0.676

5.339 ± 0.791

4.831 ± 0.562

8.475 ± 1.646

6.949 ± 0.859

7.119 ± 2.028

7.203 ± 1.507

1.017 ± 0.276

3.136 ± 0.676
Note: For amino acid frequency statistics the error has been estimated with the bootstraping (x100) at the protein level

Most of the basic statistics you can see at this page can be downloaded from this CSV file

For dipeptide frequency statistics click here
See this proteome in: uniprot_link
Proteome-pI is available under Creative Commons Attribution-NoDerivs license, for more details see here

Reference: Kozlowski LP. Proteome-pI 2.0: Proteome Isoelectric Point Database Update. Nucleic Acids Res. 2021, doi: 10.1093/nar/gkab944 Contact: Lukasz P. Kozlowski