Candidatus Nasuia deltocephalinicola

Taxonomy: cellular organisms; Bacteria; Proteobacteria; Betaproteobacteria; Betaproteobacteria incertae sedis; Candidatus Nasuia

Average proteome isoelectric point is 8.8

Get precalculated fractions of proteins

Acidic
pI < 6.8
6.8-7.4
pI > 7.4
Basic
    
All



Virtual 2D-PAGE plot for 159 proteins (isoelectric point calculated using IPC2_protein)

Get csv file with sequences according to given criteria:
        -     Method 

     -  kDa    
                                                                                      

* You can choose from 21 different methods for calculating isoelectric point

Summary statistics related to proteome-wise predictions


    
Protein with the lowest isoelectric point:
>tr|A0A286QUW8|A0A286QUW8_9PROT Sulfite reductase [NADPH] hemoprotein beta-component OS=Candidatus Nasuia deltocephalinicola OX=1160784 GN=CA212_118 PE=4 SV=1
MM1 pKa = 7.4SKK3 pKa = 10.24IIGIDD8 pKa = 3.1LGTTNSCVAFMDD20 pKa = 4.29GDD22 pKa = 3.81QVKK25 pKa = 10.6VIEE28 pKa = 4.28NSEE31 pKa = 4.1GSRR34 pKa = 11.84ITPSVVAYY42 pKa = 10.14LPNNEE47 pKa = 4.22ILVGIPAKK55 pKa = 10.14RR56 pKa = 11.84QSITNPKK63 pKa = 7.33NTIYY67 pKa = 10.52AVKK70 pKa = 10.17RR71 pKa = 11.84IIGRR75 pKa = 11.84RR76 pKa = 11.84FLEE79 pKa = 4.52KK80 pKa = 10.23EE81 pKa = 3.84VQKK84 pKa = 11.04NINSVPYY91 pKa = 10.35DD92 pKa = 3.19IVEE95 pKa = 4.47ADD97 pKa = 5.48NGDD100 pKa = 3.09AWIKK104 pKa = 10.36IDD106 pKa = 3.9EE107 pKa = 4.55KK108 pKa = 11.04KK109 pKa = 10.22IAPQQISAEE118 pKa = 4.13ILKK121 pKa = 10.55KK122 pKa = 10.19MKK124 pKa = 9.55FTVEE128 pKa = 4.27EE129 pKa = 4.18YY130 pKa = 10.49LGSNIEE136 pKa = 4.17EE137 pKa = 4.1AVVTVPAYY145 pKa = 10.72FNDD148 pKa = 3.76SQRR151 pKa = 11.84QATKK155 pKa = 10.65DD156 pKa = 3.08AGLIAGLNIKK166 pKa = 10.27RR167 pKa = 11.84IINEE171 pKa = 3.61PTAAALAFGLDD182 pKa = 3.31KK183 pKa = 11.47VSINGSKK190 pKa = 9.88IVIYY194 pKa = 10.45DD195 pKa = 3.79LGGGTFDD202 pKa = 3.9ISIIEE207 pKa = 3.84ITEE210 pKa = 3.64IDD212 pKa = 3.74GEE214 pKa = 4.39KK215 pKa = 10.3QFEE218 pKa = 4.19VLATNGDD225 pKa = 3.87TFLGGEE231 pKa = 4.69DD232 pKa = 3.41FDD234 pKa = 4.12QRR236 pKa = 11.84IINYY240 pKa = 8.93VLEE243 pKa = 4.48KK244 pKa = 10.59FKK246 pKa = 11.03EE247 pKa = 4.11DD248 pKa = 3.81NGIDD252 pKa = 3.62LSKK255 pKa = 11.2DD256 pKa = 3.14VLALQRR262 pKa = 11.84LKK264 pKa = 11.02DD265 pKa = 3.65VSEE268 pKa = 4.02KK269 pKa = 11.19AKK271 pKa = 10.57IEE273 pKa = 4.12LSTILQTDD281 pKa = 4.06LSLPYY286 pKa = 9.09ITADD290 pKa = 2.99AYY292 pKa = 10.68GPKK295 pKa = 9.8HH296 pKa = 6.52LNYY299 pKa = 10.17KK300 pKa = 9.86INRR303 pKa = 11.84STLEE307 pKa = 3.85SLVIDD312 pKa = 5.36LIEE315 pKa = 4.43STLKK319 pKa = 10.18PCEE322 pKa = 3.76IAIKK326 pKa = 10.51DD327 pKa = 3.55SGINISDD334 pKa = 3.47IKK336 pKa = 11.13DD337 pKa = 3.51VILVGGQTRR346 pKa = 11.84MPKK349 pKa = 10.22VIQKK353 pKa = 9.95VKK355 pKa = 10.53EE356 pKa = 4.11FFSKK360 pKa = 10.75DD361 pKa = 3.07PRR363 pKa = 11.84RR364 pKa = 11.84DD365 pKa = 3.34INPDD369 pKa = 2.88EE370 pKa = 4.33AVAIGAAIQGQVLSGEE386 pKa = 4.34RR387 pKa = 11.84KK388 pKa = 10.26DD389 pKa = 4.72ILLLDD394 pKa = 3.86VTPLSLGIEE403 pKa = 4.29TLGGVMTKK411 pKa = 9.95MINKK415 pKa = 7.12NTTIPTRR422 pKa = 11.84YY423 pKa = 8.35SQVYY427 pKa = 7.96STADD431 pKa = 3.54DD432 pKa = 3.85NQSSVTIRR440 pKa = 11.84VYY442 pKa = 10.49QGEE445 pKa = 4.42RR446 pKa = 11.84DD447 pKa = 3.55MALHH451 pKa = 6.36NKK453 pKa = 9.29LLAEE457 pKa = 4.93FSLEE461 pKa = 4.38GIPPAARR468 pKa = 11.84GVPQIEE474 pKa = 4.23VTFDD478 pKa = 2.94IDD480 pKa = 4.14SNGILSVKK488 pKa = 10.62AKK490 pKa = 10.8DD491 pKa = 3.6KK492 pKa = 10.6LTLKK496 pKa = 10.03EE497 pKa = 3.88NKK499 pKa = 8.43ITIKK503 pKa = 10.92ANTGLSDD510 pKa = 3.83AEE512 pKa = 3.89IDD514 pKa = 4.81KK515 pKa = 10.15MIKK518 pKa = 10.14DD519 pKa = 3.76AEE521 pKa = 4.41LNMEE525 pKa = 4.15EE526 pKa = 4.21DD527 pKa = 3.72KK528 pKa = 11.05KK529 pKa = 11.02IKK531 pKa = 10.26EE532 pKa = 4.24LTEE535 pKa = 4.12SRR537 pKa = 11.84NKK539 pKa = 10.19AQEE542 pKa = 4.32LIYY545 pKa = 9.63NTKK548 pKa = 10.48KK549 pKa = 10.84SLNEE553 pKa = 4.06YY554 pKa = 10.3GNKK557 pKa = 9.64LQPNEE562 pKa = 4.39LSDD565 pKa = 3.95INLKK569 pKa = 10.6INDD572 pKa = 4.28LEE574 pKa = 4.36ILLSNVKK581 pKa = 8.3STKK584 pKa = 10.26SEE586 pKa = 3.17IDD588 pKa = 3.45YY589 pKa = 11.09KK590 pKa = 11.1VDD592 pKa = 3.38EE593 pKa = 4.85LLKK596 pKa = 10.59SSQKK600 pKa = 8.74MGEE603 pKa = 4.28RR604 pKa = 11.84IYY606 pKa = 11.26SPNSSNNYY614 pKa = 10.03SDD616 pKa = 3.42INNNFDD622 pKa = 3.55INNNDD627 pKa = 3.43FSRR630 pKa = 11.84DD631 pKa = 3.33NNYY634 pKa = 9.43TGNNDD639 pKa = 3.24EE640 pKa = 5.24NSNDD644 pKa = 3.86KK645 pKa = 11.47NNDD648 pKa = 3.19EE649 pKa = 5.21NNDD652 pKa = 3.57EE653 pKa = 5.07NNDD656 pKa = 3.65EE657 pKa = 5.07NNDD660 pKa = 3.65EE661 pKa = 5.07NNDD664 pKa = 3.65EE665 pKa = 5.07NNDD668 pKa = 3.65EE669 pKa = 5.07NNDD672 pKa = 3.65EE673 pKa = 5.07NNDD676 pKa = 3.65EE677 pKa = 5.07NNDD680 pKa = 3.65EE681 pKa = 5.07NNDD684 pKa = 3.73EE685 pKa = 4.43NNDD688 pKa = 4.11IIDD691 pKa = 4.3NYY693 pKa = 10.87DD694 pKa = 2.96NSYY697 pKa = 11.12LEE699 pKa = 4.33KK700 pKa = 10.68KK701 pKa = 10.18

Molecular weight: 78.29 kDa
Isoelectric point according different methods:





Protein with the highest isoelectric point:
>tr|A0A248QFN0|A0A248QFN0_9PROT 50S ribosomal protein L27p OS=Candidatus Nasuia deltocephalinicola OX=1160784 GN=CA212_071 PE=3 SV=1
MM1 pKa = 7.58RR2 pKa = 11.84HH3 pKa = 5.45LKK5 pKa = 10.41KK6 pKa = 9.88RR7 pKa = 11.84DD8 pKa = 3.34KK9 pKa = 11.3LGIKK13 pKa = 9.83YY14 pKa = 8.41SHH16 pKa = 6.76KK17 pKa = 10.51KK18 pKa = 10.36SLISNLSNSLIKK30 pKa = 10.68YY31 pKa = 8.01EE32 pKa = 3.91YY33 pKa = 10.93LKK35 pKa = 9.6ITFVRR40 pKa = 11.84AKK42 pKa = 9.6VLKK45 pKa = 10.63SFLEE49 pKa = 4.5PIITVSKK56 pKa = 10.94YY57 pKa = 8.99FFNYY61 pKa = 9.15NVRR64 pKa = 11.84FVFKK68 pKa = 10.55KK69 pKa = 10.29LRR71 pKa = 11.84NTEE74 pKa = 4.15SVLKK78 pKa = 9.73LFKK81 pKa = 10.54IIGPRR86 pKa = 11.84YY87 pKa = 7.77FFRR90 pKa = 11.84NGGYY94 pKa = 9.81LQIFKK99 pKa = 10.67KK100 pKa = 10.56GFRR103 pKa = 11.84RR104 pKa = 11.84GDD106 pKa = 3.63NACLALIKK114 pKa = 10.64LII116 pKa = 4.38

Molecular weight: 13.83 kDa
Isoelectric point according different methods:





Peptides (in silico digests for buttom-up proteomics)

Below you can find in silico digests of the whole proteome with Trypsin, Chymotrypsin, Trypsin+LysC, LysN, ArgC proteases suitable for different mass spec machines.

Try
ESI
ChTry
ESI
ArgC
ESI
LysN
ESI
TryLysC
ESI

Try
MALDI
ChTry
MALDI
ArgC
MALDI
LysN
MALDI
TryLysC
MALDI

Try
LTQ
ChTry
LTQ
ArgC
LTQ
LysN
LTQ
TryLysC
LTQ

Try
MSlow
ChTry
MSlow
ArgC
MSlow
LysN
MSlow
TryLysC
MSlow

Try
MShigh
ChTry
MShigh
ArgC
MShigh
LysN
MShigh
TryLysC
MShigh

General Statistics

Number of major isoforms

Number of additional isoforms

Number of all proteins

Number of amino acids

Min. Seq. Length

Max. Seq. Length

Avg. Seq. Length

Avg. Mol. Weight

159

0

159

43276

37

1361

272.2

32.4

Amino acid frequency

Ala

Cys

Asp

Glu

Phe

Gly

His

Ile

Lys

Leu

1.257 ± 0.132

1.174 ± 0.056

3.358 ± 0.215

3.187 ± 0.213

9.315 ± 0.503

3.632 ± 0.211

0.915 ± 0.063

15.452 ± 0.266

14.424 ± 0.452

10.59 ± 0.191

Met

Asn

Gln

Pro

Arg

Ser

Thr

Val

Trp

Tyr

1.135 ± 0.066

12.631 ± 0.295

1.752 ± 0.085

1.109 ± 0.067

2.068 ± 0.103

6.304 ± 0.216

2.308 ± 0.12

2.537 ± 0.137

0.525 ± 0.061

6.329 ± 0.24
Note: For amino acid frequency statistics the error has been estimated with the bootstraping (x100) at the protein level

Most of the basic statistics you can see at this page can be downloaded from this CSV file

For dipeptide frequency statistics click here
See this proteome in: uniprot_link
Proteome-pI is available under Creative Commons Attribution-NoDerivs license, for more details see here

Reference: Kozlowski LP. Proteome-pI 2.0: Proteome Isoelectric Point Database Update. Nucleic Acids Res. 2021, doi: 10.1093/nar/gkab944 Contact: Lukasz P. Kozlowski