Microbacterium mitrae

Taxonomy: cellular organisms; Bacteria; Terrabacteria group; Actinobacteria; Actinomycetia; Micrococcales; Microbacteriaceae; Microbacterium

Average proteome isoelectric point is 5.92

Get precalculated fractions of proteins

Acidic
pI < 6.8
6.8-7.4
pI > 7.4
Basic
    
All



Virtual 2D-PAGE plot for 2481 proteins (isoelectric point calculated using IPC2_protein)

Get csv file with sequences according to given criteria:
        -     Method 

     -  kDa    
                                                                                      

* You can choose from 21 different methods for calculating isoelectric point

Summary statistics related to proteome-wise predictions


    
Protein with the lowest isoelectric point:
>tr|A0A5C8HJV4|A0A5C8HJV4_9MICO Phosphoribosylformylglycinamidine synthase subunit PurL OS=Microbacterium mitrae OX=664640 GN=purL PE=3 SV=1
MM1 pKa = 8.55SMEE4 pKa = 4.07GRR6 pKa = 11.84FGLKK10 pKa = 9.95AGAVVTFAALIACTSAATTSFAAEE34 pKa = 4.1PTEE37 pKa = 4.1VSIPTPQNLEE47 pKa = 3.9DD48 pKa = 3.28GRR50 pKa = 11.84YY51 pKa = 9.07IVLLDD56 pKa = 4.17DD57 pKa = 5.15APAATYY63 pKa = 10.29EE64 pKa = 4.21GGEE67 pKa = 4.32AGLTATKK74 pKa = 9.7PAEE77 pKa = 4.22GDD79 pKa = 3.56KK80 pKa = 11.28LDD82 pKa = 3.77ATTDD86 pKa = 3.37AVVEE90 pKa = 4.22YY91 pKa = 10.57SSHH94 pKa = 7.24LEE96 pKa = 3.87NLQDD100 pKa = 3.41EE101 pKa = 4.87VAAEE105 pKa = 4.24VGATPDD111 pKa = 3.37ANLTITLNAFTANLTASQAKK131 pKa = 9.13QLAATNGVSAVVPDD145 pKa = 5.13EE146 pKa = 4.24ILHH149 pKa = 5.37PTAVPSTEE157 pKa = 3.88FLGLEE162 pKa = 4.16GDD164 pKa = 3.55NGVWEE169 pKa = 4.86TYY171 pKa = 10.84AGGPEE176 pKa = 4.1GAGEE180 pKa = 4.3GVVVGVIDD188 pKa = 3.79TGIAPEE194 pKa = 4.21NPSFAGEE201 pKa = 4.03PLGTTSGATPYY212 pKa = 11.03LEE214 pKa = 4.37GNTVVFNKK222 pKa = 10.38ADD224 pKa = 3.64GGQFRR229 pKa = 11.84SDD231 pKa = 3.7RR232 pKa = 11.84VTGDD236 pKa = 2.78QWNLNDD242 pKa = 4.25YY243 pKa = 7.43STKK246 pKa = 10.69LIAAHH251 pKa = 5.88YY252 pKa = 8.67FSAGAAASGFDD263 pKa = 3.98FQWDD267 pKa = 3.36ILSPRR272 pKa = 11.84DD273 pKa = 3.59GAGHH277 pKa = 6.87GSHH280 pKa = 6.47TASTAAGNYY289 pKa = 8.58NVEE292 pKa = 4.45TTVSGLDD299 pKa = 3.38FGPISGVAPAAKK311 pKa = 8.79IASYY315 pKa = 8.09KK316 pKa = 8.83TCYY319 pKa = 9.61EE320 pKa = 4.08GQDD323 pKa = 3.67PAVTTDD329 pKa = 4.76DD330 pKa = 3.32ICAGSDD336 pKa = 3.21LLAAIDD342 pKa = 3.94RR343 pKa = 11.84ATKK346 pKa = 10.64DD347 pKa = 3.37GVDD350 pKa = 3.95VINFSIGGGAATTVWDD366 pKa = 4.57ALDD369 pKa = 3.28QAFFNAATAGIFVSASAGNSGPDD392 pKa = 3.71YY393 pKa = 10.21VTADD397 pKa = 4.08HH398 pKa = 7.06ASPWYY403 pKa = 6.52TTVAASTIPTYY414 pKa = 10.58EE415 pKa = 3.81GTVVGEE421 pKa = 4.19GFEE424 pKa = 4.58APGATVSVPQGGEE437 pKa = 3.85VTGDD441 pKa = 3.78AIYY444 pKa = 10.76AGDD447 pKa = 3.74VAASGATAANAALCLPNTLDD467 pKa = 3.43ASLVAGKK474 pKa = 10.05IVVCDD479 pKa = 3.62RR480 pKa = 11.84GSNARR485 pKa = 11.84VDD487 pKa = 3.43KK488 pKa = 10.75SANVQAAGGIGMVLVNVGPGSLDD511 pKa = 3.1TDD513 pKa = 3.58MHH515 pKa = 6.48SVPTVHH521 pKa = 7.49IADD524 pKa = 4.46TYY526 pKa = 10.98RR527 pKa = 11.84ADD529 pKa = 3.88LLATITEE536 pKa = 4.43TPEE539 pKa = 3.53ITLLPQNITDD549 pKa = 4.62FVTPVPQIAGFSSRR563 pKa = 11.84GPMMADD569 pKa = 2.98GSDD572 pKa = 3.03ILKK575 pKa = 10.09PDD577 pKa = 3.14IAAPGVNILAAVNNVEE593 pKa = 4.9DD594 pKa = 5.46AAPQWGLMSGTSMAAPHH611 pKa = 6.44IAGLAALYY619 pKa = 10.43LGEE622 pKa = 4.98RR623 pKa = 11.84PQATPGEE630 pKa = 4.34IKK632 pKa = 10.39SALMTTAYY640 pKa = 8.77DD641 pKa = 3.83TVDD644 pKa = 4.35SEE646 pKa = 5.1DD647 pKa = 4.11ASNPDD652 pKa = 3.73PFAQGAGQVDD662 pKa = 3.39AKK664 pKa = 11.04LYY666 pKa = 10.66FNAGLLYY673 pKa = 10.98LNGPADD679 pKa = 3.19WAAYY683 pKa = 10.52LEE685 pKa = 4.53GKK687 pKa = 9.77GLYY690 pKa = 9.79EE691 pKa = 4.07FDD693 pKa = 3.39GVEE696 pKa = 4.71PIDD699 pKa = 5.08GSDD702 pKa = 3.92LNLASISIGSLAGPQTVTRR721 pKa = 11.84TVTAQTAGTYY731 pKa = 7.66EE732 pKa = 3.88ATIDD736 pKa = 3.76VPGVTTVVEE745 pKa = 4.35PSSITLAAGEE755 pKa = 4.5SATFTVSFEE764 pKa = 4.02NATAPTEE771 pKa = 3.87QWATGFLTWSSDD783 pKa = 3.8TIDD786 pKa = 3.57VRR788 pKa = 11.84SPIAVFPTAADD799 pKa = 3.71APLEE803 pKa = 4.07LYY805 pKa = 11.0GEE807 pKa = 4.49GVEE810 pKa = 5.01GVIDD814 pKa = 3.75YY815 pKa = 10.73SITPGYY821 pKa = 10.61DD822 pKa = 2.98GEE824 pKa = 4.91LPLNLSGLTPYY835 pKa = 10.39VLHH838 pKa = 7.59EE839 pKa = 4.7DD840 pKa = 3.46TDD842 pKa = 4.23NPVPGHH848 pKa = 6.41SGNQDD853 pKa = 3.0SGDD856 pKa = 3.56ADD858 pKa = 3.9GEE860 pKa = 4.1IGFILEE866 pKa = 4.21VQEE869 pKa = 4.23GAEE872 pKa = 4.18LARR875 pKa = 11.84IDD877 pKa = 5.37LDD879 pKa = 3.79SSDD882 pKa = 5.15DD883 pKa = 3.82AGSDD887 pKa = 3.45LDD889 pKa = 3.71LTVYY893 pKa = 10.65HH894 pKa = 6.62LVSPTSYY901 pKa = 10.65DD902 pKa = 3.09KK903 pKa = 11.16KK904 pKa = 11.06YY905 pKa = 10.8DD906 pKa = 3.77AQTGSADD913 pKa = 3.67EE914 pKa = 4.19QVTIHH919 pKa = 6.73APAAGTYY926 pKa = 9.77FILVDD931 pKa = 4.12MYY933 pKa = 11.21SVSGPMTWDD942 pKa = 2.89LTTAVVDD949 pKa = 4.73GAADD953 pKa = 3.85GALTATPNPVAATKK967 pKa = 10.44GVSTPVSLSWAGLEE981 pKa = 3.78NDD983 pKa = 3.36QRR985 pKa = 11.84YY986 pKa = 9.37LGYY989 pKa = 10.25VQYY992 pKa = 11.15GDD994 pKa = 4.01SAVSTIVTVDD1004 pKa = 3.29AGAAAPANTAAPVLSGSGVAGEE1026 pKa = 4.53TLTVTPGEE1034 pKa = 4.03WDD1036 pKa = 3.2QDD1038 pKa = 3.47GLTFKK1043 pKa = 11.33YY1044 pKa = 9.73EE1045 pKa = 3.68WLKK1048 pKa = 11.03NGEE1051 pKa = 4.5AIAVADD1057 pKa = 4.26APAGVNAVVPPDD1069 pKa = 3.54ATDD1072 pKa = 3.02SSTYY1076 pKa = 9.99TPTAADD1082 pKa = 3.29VGATISVRR1090 pKa = 11.84VYY1092 pKa = 10.82ASAEE1096 pKa = 4.04GNPNAGTADD1105 pKa = 3.51SNGIVITAAPTTSPTPTVDD1124 pKa = 3.13PTDD1127 pKa = 3.71PTNPTVAPTTAPTTPGAGGLPVTGAPDD1154 pKa = 3.24ATLPLLVGFLIVALGGAAFVIGRR1177 pKa = 11.84KK1178 pKa = 9.11RR1179 pKa = 11.84RR1180 pKa = 11.84AHH1182 pKa = 6.68AEE1184 pKa = 3.76

Molecular weight: 120.25 kDa
Isoelectric point according different methods:





Protein with the highest isoelectric point:
>tr|A0A5C8HLN7|A0A5C8HLN7_9MICO Penicillin-insensitive transglycosylase OS=Microbacterium mitrae OX=664640 GN=FVP60_12320 PE=4 SV=1
MM1 pKa = 7.46RR2 pKa = 11.84HH3 pKa = 5.85APSTGTRR10 pKa = 11.84RR11 pKa = 11.84PPSRR15 pKa = 11.84PARR18 pKa = 11.84FRR20 pKa = 11.84PCGLPATPQRR30 pKa = 11.84RR31 pKa = 11.84RR32 pKa = 11.84RR33 pKa = 11.84ARR35 pKa = 11.84VVPSARR41 pKa = 11.84RR42 pKa = 11.84ARR44 pKa = 11.84AVPQGAGRR52 pKa = 11.84RR53 pKa = 11.84PRR55 pKa = 11.84RR56 pKa = 11.84ARR58 pKa = 11.84RR59 pKa = 11.84ARR61 pKa = 11.84WWRR64 pKa = 3.35

Molecular weight: 7.49 kDa
Isoelectric point according different methods:





Peptides (in silico digests for buttom-up proteomics)

Below you can find in silico digests of the whole proteome with Trypsin, Chymotrypsin, Trypsin+LysC, LysN, ArgC proteases suitable for different mass spec machines.

Try
ESI
ChTry
ESI
ArgC
ESI
LysN
ESI
TryLysC
ESI

Try
MALDI
ChTry
MALDI
ArgC
MALDI
LysN
MALDI
TryLysC
MALDI

Try
LTQ
ChTry
LTQ
ArgC
LTQ
LysN
LTQ
TryLysC
LTQ

Try
MSlow
ChTry
MSlow
ArgC
MSlow
LysN
MSlow
TryLysC
MSlow

Try
MShigh
ChTry
MShigh
ArgC
MShigh
LysN
MShigh
TryLysC
MShigh

General Statistics

Number of major isoforms

Number of additional isoforms

Number of all proteins

Number of amino acids

Min. Seq. Length

Max. Seq. Length

Avg. Seq. Length

Avg. Mol. Weight

2481

0

2481

825282

29

3744

332.6

35.69

Amino acid frequency

Ala

Cys

Asp

Glu

Phe

Gly

His

Ile

Lys

Leu

13.318 ± 0.072

0.496 ± 0.015

6.055 ± 0.041

5.794 ± 0.051

3.231 ± 0.03

8.417 ± 0.044

1.976 ± 0.025

5.139 ± 0.038

2.336 ± 0.036

9.643 ± 0.062

Met

Asn

Gln

Pro

Arg

Ser

Thr

Val

Trp

Tyr

2.07 ± 0.024

2.361 ± 0.027

5.077 ± 0.034

3.009 ± 0.025

6.662 ± 0.058

5.712 ± 0.033

6.457 ± 0.066

8.745 ± 0.048

1.452 ± 0.021

2.051 ± 0.023
Note: For amino acid frequency statistics the error has been estimated with the bootstraping (x100) at the protein level

Most of the basic statistics you can see at this page can be downloaded from this CSV file

For dipeptide frequency statistics click here
See this proteome in: uniprot_link
Proteome-pI is available under Creative Commons Attribution-NoDerivs license, for more details see here

Reference: Kozlowski LP. Proteome-pI 2.0: Proteome Isoelectric Point Database Update. Nucleic Acids Res. 2021, doi: 10.1093/nar/gkab944 Contact: Lukasz P. Kozlowski