Streptococcus phage phi-SsuHCJ3_rum

Taxonomy: Viruses; Duplodnaviria; Heunggongvirae; Uroviricota; Caudoviricetes; Caudovirales; Siphoviridae; unclassified Siphoviridae

Average proteome isoelectric point is 6.18

Get precalculated fractions of proteins

Acidic
pI < 6.8
6.8-7.4
pI > 7.4
Basic
    
All



Virtual 2D-PAGE plot for 66 proteins (isoelectric point calculated using IPC2_protein)

Get csv file with sequences according to given criteria:
        -     Method 

     -  kDa    
                                                                                      

* You can choose from 21 different methods for calculating isoelectric point

Summary statistics related to proteome-wise predictions


    
Protein with the lowest isoelectric point:
>tr|A0A7G3MTA4|A0A7G3MTA4_9CAUD Uncharacterized protein OS=Streptococcus phage phi-SsuHCJ3_rum OX=2664399 PE=4 SV=1
MM1 pKa = 7.36WEE3 pKa = 3.5IMTRR7 pKa = 11.84TVEE10 pKa = 3.94DD11 pKa = 2.86RR12 pKa = 11.84HH13 pKa = 5.43YY14 pKa = 9.88VCEE17 pKa = 4.06FLRR20 pKa = 11.84EE21 pKa = 4.16DD22 pKa = 3.43TTDD25 pKa = 3.05PRR27 pKa = 11.84NIDD30 pKa = 3.65GAWISILTIKK40 pKa = 10.47RR41 pKa = 11.84DD42 pKa = 3.22GEE44 pKa = 4.16YY45 pKa = 9.72IYY47 pKa = 10.36QYY49 pKa = 10.86RR50 pKa = 11.84YY51 pKa = 10.77GNEE54 pKa = 3.93IDD56 pKa = 4.6NMDD59 pKa = 5.1DD60 pKa = 3.19IDD62 pKa = 3.95RR63 pKa = 11.84TVCQAVLDD71 pKa = 4.16NFNEE75 pKa = 4.18LL76 pKa = 3.58

Molecular weight: 9.2 kDa
Isoelectric point according different methods:





Protein with the highest isoelectric point:
>tr|A0A7G3MJR6|A0A7G3MJR6_9CAUD Plasmid stabilization system antitoxin protein OS=Streptococcus phage phi-SsuHCJ3_rum OX=2664399 PE=4 SV=1
MM1 pKa = 7.74PRR3 pKa = 11.84RR4 pKa = 11.84PSTPCKK10 pKa = 10.41QNGCPNLVPYY20 pKa = 8.23GHH22 pKa = 7.43RR23 pKa = 11.84YY24 pKa = 8.07CANHH28 pKa = 6.97KK29 pKa = 10.52ANHH32 pKa = 5.63QLDD35 pKa = 3.98AKK37 pKa = 8.36STKK40 pKa = 10.46AKK42 pKa = 10.27GYY44 pKa = 7.14NARR47 pKa = 11.84WNKK50 pKa = 9.24VRR52 pKa = 11.84LRR54 pKa = 11.84YY55 pKa = 10.08LKK57 pKa = 9.81VHH59 pKa = 6.5PLCFQCKK66 pKa = 9.66AKK68 pKa = 10.55GRR70 pKa = 11.84LTKK73 pKa = 10.1ATVVDD78 pKa = 5.28HH79 pKa = 6.23ITPHH83 pKa = 6.97RR84 pKa = 11.84GDD86 pKa = 5.06QEE88 pKa = 5.0LFWNQTNWQALCKK101 pKa = 9.93SCHH104 pKa = 5.64DD105 pKa = 4.22RR106 pKa = 11.84KK107 pKa = 8.93TKK109 pKa = 8.45TTDD112 pKa = 2.85LYY114 pKa = 11.62VEE116 pKa = 3.8YY117 pKa = 9.3TYY119 pKa = 11.24RR120 pKa = 11.84FF121 pKa = 3.55

Molecular weight: 14.25 kDa
Isoelectric point according different methods:





Peptides (in silico digests for buttom-up proteomics)

Below you can find in silico digests of the whole proteome with Trypsin, Chymotrypsin, Trypsin+LysC, LysN, ArgC proteases suitable for different mass spec machines.

Try
ESI
ChTry
ESI
ArgC
ESI
LysN
ESI
TryLysC
ESI

Try
MALDI
ChTry
MALDI
ArgC
MALDI
LysN
MALDI
TryLysC
MALDI

Try
LTQ
ChTry
LTQ
ArgC
LTQ
LysN
LTQ
TryLysC
LTQ

Try
MSlow
ChTry
MSlow
ArgC
MSlow
LysN
MSlow
TryLysC
MSlow

Try
MShigh
ChTry
MShigh
ArgC
MShigh
LysN
MShigh
TryLysC
MShigh

General Statistics

Number of major isoforms

Number of additional isoforms

Number of all proteins

Number of amino acids

Min. Seq. Length

Max. Seq. Length

Avg. Seq. Length

Avg. Mol. Weight

66

0

66

16976

33

1515

257.2

29.14

Amino acid frequency

Ala

Cys

Asp

Glu

Phe

Gly

His

Ile

Lys

Leu

6.315 ± 0.371

0.96 ± 0.125

5.961 ± 0.232

7.363 ± 0.301

3.935 ± 0.191

6.556 ± 0.283

2.097 ± 0.171

6.992 ± 0.26

7.363 ± 0.251

9.054 ± 0.269

Met

Asn

Gln

Pro

Arg

Ser

Thr

Val

Trp

Tyr

2.486 ± 0.175

4.842 ± 0.211

2.987 ± 0.188

4.271 ± 0.234

4.654 ± 0.228

6.568 ± 0.343

6.162 ± 0.465

6.203 ± 0.204

1.308 ± 0.088

3.923 ± 0.299
Note: For amino acid frequency statistics the error has been estimated with the bootstraping (x100) at the protein level

Most of the basic statistics you can see at this page can be downloaded from this CSV file

For dipeptide frequency statistics click here
See this proteome in: uniprot_link
Proteome-pI is available under Creative Commons Attribution-NoDerivs license, for more details see here

Reference: Kozlowski LP. Proteome-pI 2.0: Proteome Isoelectric Point Database Update. Nucleic Acids Res. 2021, doi: 10.1093/nar/gkab944 Contact: Lukasz P. Kozlowski