Microbacterium sp. XT11

Taxonomy: cellular organisms; Bacteria; Terrabacteria group; Actinobacteria; Actinomycetia; Micrococcales; Microbacteriaceae; Microbacterium; unclassified Microbacterium

Average proteome isoelectric point is 5.92

Get precalculated fractions of proteins

Acidic
pI < 6.8
6.8-7.4
pI > 7.4
Basic
    
All



Virtual 2D-PAGE plot for 1927 proteins (isoelectric point calculated using IPC2_protein)

Get csv file with sequences according to given criteria:
        -     Method 

     -  kDa    
                                                                                      

* You can choose from 21 different methods for calculating isoelectric point

Summary statistics related to proteome-wise predictions


    
Protein with the lowest isoelectric point:
>tr|A0A0U4H705|A0A0U4H705_9MICO o-succinylbenzoate synthase OS=Microbacterium sp. XT11 OX=367477 GN=menC PE=3 SV=1
MM1 pKa = 7.06ATSTRR6 pKa = 11.84ALIGRR11 pKa = 11.84RR12 pKa = 11.84RR13 pKa = 11.84PAARR17 pKa = 11.84PGRR20 pKa = 11.84SGEE23 pKa = 3.78RR24 pKa = 11.84RR25 pKa = 11.84RR26 pKa = 11.84AGVAAAIVAALAVSLLGVALPSPALAAGNTPPSNAYY62 pKa = 10.06SNQWLYY68 pKa = 11.42AYY70 pKa = 10.33VEE72 pKa = 4.18AGEE75 pKa = 4.53TVVGTGNIEE84 pKa = 4.0QVVAPDD90 pKa = 3.54GTTALGPGTYY100 pKa = 10.34GPAAADD106 pKa = 3.91GVWRR110 pKa = 11.84IHH112 pKa = 6.68LPSQGTSIGYY122 pKa = 8.39SWTVDD127 pKa = 2.64VHH129 pKa = 6.45AAGSGQVIPGRR140 pKa = 11.84VWSDD144 pKa = 2.77RR145 pKa = 11.84YY146 pKa = 11.11YY147 pKa = 9.23MQQASGTANTVDD159 pKa = 3.63LNFWIVNNTGYY170 pKa = 10.98VYY172 pKa = 10.69AVEE175 pKa = 4.49LDD177 pKa = 4.12DD178 pKa = 4.22YY179 pKa = 11.55NGVNSIFEE187 pKa = 4.48ANSVGWADD195 pKa = 4.02EE196 pKa = 4.26ADD198 pKa = 4.9CIPPYY203 pKa = 10.76ASYY206 pKa = 10.4EE207 pKa = 4.05PPGGVGTTLPLPDD220 pKa = 4.66CGEE223 pKa = 4.11TYY225 pKa = 10.43RR226 pKa = 11.84VFFEE230 pKa = 4.84EE231 pKa = 4.7PADD234 pKa = 4.33DD235 pKa = 4.56LPQVSSTNLGSVNVRR250 pKa = 11.84PDD252 pKa = 3.52LLGPADD258 pKa = 4.32LAVDD262 pKa = 4.23DD263 pKa = 5.33LAFAPAGGGSATGTFTYY280 pKa = 10.36SINDD284 pKa = 3.82RR285 pKa = 11.84FTGGYY290 pKa = 9.12VLQIDD295 pKa = 3.75VDD297 pKa = 4.06GDD299 pKa = 3.64GSYY302 pKa = 11.07DD303 pKa = 3.59GQRR306 pKa = 11.84DD307 pKa = 3.18RR308 pKa = 11.84SIRR311 pKa = 11.84LGADD315 pKa = 3.02GSGSYY320 pKa = 10.44SYY322 pKa = 11.59AFDD325 pKa = 4.11GLDD328 pKa = 3.41GLGDD332 pKa = 4.56PIEE335 pKa = 4.84DD336 pKa = 4.05CTRR339 pKa = 11.84MGARR343 pKa = 11.84VFFDD347 pKa = 3.22KK348 pKa = 10.95LGEE351 pKa = 4.04VHH353 pKa = 7.37ILQTDD358 pKa = 3.58VEE360 pKa = 4.59GRR362 pKa = 11.84AGISIRR368 pKa = 11.84RR369 pKa = 11.84LNGAGAPDD377 pKa = 3.55STIYY381 pKa = 10.37WDD383 pKa = 3.6DD384 pKa = 3.41TQMTGARR391 pKa = 11.84VNTTPVLSATDD402 pKa = 4.0GADD405 pKa = 3.39SAGGAHH411 pKa = 6.81GWAYY415 pKa = 10.98DD416 pKa = 3.61NNSWGNEE423 pKa = 3.62RR424 pKa = 11.84VIDD427 pKa = 3.77DD428 pKa = 3.89WTYY431 pKa = 11.11LPADD435 pKa = 4.63FGTGEE440 pKa = 3.84IQFGGRR446 pKa = 11.84CLSIDD451 pKa = 3.34KK452 pKa = 10.51TSDD455 pKa = 3.13FTEE458 pKa = 4.55DD459 pKa = 3.33SRR461 pKa = 11.84PGDD464 pKa = 3.45VVAYY468 pKa = 8.14TVTATNVGDD477 pKa = 3.61TDD479 pKa = 3.92YY480 pKa = 11.08TVADD484 pKa = 3.98PAVLFDD490 pKa = 5.02DD491 pKa = 4.98LSGVLDD497 pKa = 4.16DD498 pKa = 4.86ATYY501 pKa = 11.33NDD503 pKa = 4.17DD504 pKa = 3.99AAVVASDD511 pKa = 4.27GDD513 pKa = 4.3DD514 pKa = 3.6VPAPAFIEE522 pKa = 4.43PSHH525 pKa = 6.46LSWAGPLAAGEE536 pKa = 4.5SVEE539 pKa = 4.09LTYY542 pKa = 10.77TVTLGSAGDD551 pKa = 3.89GEE553 pKa = 4.8VANVAWQPVTTPPPGQVPPTPLCEE577 pKa = 4.15DD578 pKa = 3.57AVDD581 pKa = 4.0GRR583 pKa = 11.84DD584 pKa = 3.42PVTGEE589 pKa = 4.01ACDD592 pKa = 3.87GTVGEE597 pKa = 4.88LPRR600 pKa = 11.84LTIAKK605 pKa = 8.55TADD608 pKa = 3.25ATTLPTDD615 pKa = 3.88GDD617 pKa = 4.01EE618 pKa = 4.21VTYY621 pKa = 10.1TVTVTNEE628 pKa = 3.78GPGFYY633 pKa = 10.01TDD635 pKa = 4.74DD636 pKa = 4.21APATMTDD643 pKa = 3.19SLEE646 pKa = 4.14EE647 pKa = 4.05VLDD650 pKa = 4.12DD651 pKa = 4.97AEE653 pKa = 4.5FGEE656 pKa = 4.63ILAPAAGAEE665 pKa = 3.99FDD667 pKa = 4.29ADD669 pKa = 3.87AEE671 pKa = 4.28EE672 pKa = 4.68LTWSGPLAAGASIEE686 pKa = 3.73IRR688 pKa = 11.84YY689 pKa = 6.67TVVYY693 pKa = 10.14DD694 pKa = 3.77ATAGDD699 pKa = 4.03SVLTNTACVPEE710 pKa = 5.66ADD712 pKa = 4.04TLPGAEE718 pKa = 3.9SCATVRR724 pKa = 11.84IPGGRR729 pKa = 11.84LAVSKK734 pKa = 10.96SVDD737 pKa = 3.5PADD740 pKa = 3.89GSTVQSGQDD749 pKa = 3.14VTYY752 pKa = 9.73TLTFEE757 pKa = 4.46STGEE761 pKa = 4.15SEE763 pKa = 4.42VTVDD767 pKa = 5.61KK768 pKa = 11.48VDD770 pKa = 4.15DD771 pKa = 4.09LSGVLDD777 pKa = 3.87DD778 pKa = 5.14AEE780 pKa = 4.25FVEE783 pKa = 5.25GSIDD787 pKa = 3.68VSDD790 pKa = 4.14AAALTAVVDD799 pKa = 4.06GDD801 pKa = 3.78EE802 pKa = 4.21LAIDD806 pKa = 3.82GTIPAGQTVTVSYY819 pKa = 9.38TVTVAPYY826 pKa = 10.39AEE828 pKa = 4.4QGDD831 pKa = 4.23HH832 pKa = 6.22VLANVVQNPDD842 pKa = 3.53GTCGPEE848 pKa = 4.12GCPGTEE854 pKa = 3.36NPIRR858 pKa = 11.84HH859 pKa = 5.68FSITKK864 pKa = 7.76TAEE867 pKa = 3.83PVDD870 pKa = 4.45DD871 pKa = 3.96VTSGDD876 pKa = 3.56VVTYY880 pKa = 9.17TVTVTNDD887 pKa = 2.63GAADD891 pKa = 3.8YY892 pKa = 7.59TTDD895 pKa = 3.43APASVTDD902 pKa = 4.55DD903 pKa = 3.77LTDD906 pKa = 3.52VLDD909 pKa = 4.25DD910 pKa = 3.77AVYY913 pKa = 10.89DD914 pKa = 4.12GGATAVSSDD923 pKa = 3.38GSAVPAPQYY932 pKa = 10.91GEE934 pKa = 4.73PVLTWSGPVAAGEE947 pKa = 4.45SVEE950 pKa = 3.84ITYY953 pKa = 9.13TVTVTNLGDD962 pKa = 3.76HH963 pKa = 7.07DD964 pKa = 4.97LVNVAAPVCAPGEE977 pKa = 4.36DD978 pKa = 4.17CDD980 pKa = 4.36PPVSVEE986 pKa = 3.79ILLPSIVPAKK996 pKa = 10.63SSDD999 pKa = 3.71PASGEE1004 pKa = 4.03SLLAGDD1010 pKa = 4.12VVDD1013 pKa = 5.27YY1014 pKa = 9.92TLSWTNDD1021 pKa = 2.91GQAAGALDD1029 pKa = 3.89STDD1032 pKa = 4.36DD1033 pKa = 3.71LTGVLDD1039 pKa = 4.79DD1040 pKa = 5.5ADD1042 pKa = 3.73ITTAPQTSADD1052 pKa = 4.0GIEE1055 pKa = 4.67LVFDD1059 pKa = 4.75PDD1061 pKa = 3.44AGTLRR1066 pKa = 11.84VTGEE1070 pKa = 4.08LAPGEE1075 pKa = 4.67TVTVTYY1081 pKa = 10.6SVTVRR1086 pKa = 11.84PDD1088 pKa = 3.04GEE1090 pKa = 4.21RR1091 pKa = 11.84GDD1093 pKa = 4.71NIVGNVLVPDD1103 pKa = 4.05VPPFEE1108 pKa = 5.41CADD1111 pKa = 4.29ADD1113 pKa = 4.15PEE1115 pKa = 4.44CDD1117 pKa = 3.46PFVPPATSHH1126 pKa = 6.65PVGEE1130 pKa = 4.21LDD1132 pKa = 3.9DD1133 pKa = 4.29WKK1135 pKa = 9.8TVDD1138 pKa = 3.72PASGATVRR1146 pKa = 11.84AGQEE1150 pKa = 3.65VTYY1153 pKa = 9.26TLHH1156 pKa = 6.41FANIGEE1162 pKa = 4.23ADD1164 pKa = 3.33VDD1166 pKa = 4.03VARR1169 pKa = 11.84EE1170 pKa = 3.94DD1171 pKa = 3.78VLTQVLDD1178 pKa = 3.87DD1179 pKa = 4.14ATVVSPPEE1187 pKa = 4.1SSDD1190 pKa = 3.2AALSVFDD1197 pKa = 3.9VADD1200 pKa = 3.54GRR1202 pKa = 11.84FLVAGTLAPGQEE1214 pKa = 3.93ATVVYY1219 pKa = 8.73TVVVNDD1225 pKa = 3.87DD1226 pKa = 3.83GEE1228 pKa = 5.37RR1229 pKa = 11.84GDD1231 pKa = 5.14DD1232 pKa = 3.21RR1233 pKa = 11.84LGNFLVDD1240 pKa = 3.85EE1241 pKa = 4.79GEE1243 pKa = 4.34EE1244 pKa = 4.16PPATCEE1250 pKa = 3.76PTDD1253 pKa = 4.57AEE1255 pKa = 4.76RR1256 pKa = 11.84ADD1258 pKa = 3.87CTVNPVSNVQVTKK1271 pKa = 10.77SADD1274 pKa = 3.6PASGTTIAYY1283 pKa = 7.49DD1284 pKa = 3.31QRR1286 pKa = 11.84VTYY1289 pKa = 9.77TLTFRR1294 pKa = 11.84NVGTNPAAAPVAVDD1308 pKa = 3.75YY1309 pKa = 10.41TDD1311 pKa = 4.01HH1312 pKa = 7.15LSGVLDD1318 pKa = 4.06DD1319 pKa = 5.33AVLSGAPAASDD1330 pKa = 3.67PSLTAAVQGQAIRR1343 pKa = 11.84ITGTVATGDD1352 pKa = 4.05VVTVTYY1358 pKa = 9.44TVAPKK1363 pKa = 10.42AYY1365 pKa = 9.55DD1366 pKa = 3.48EE1367 pKa = 4.64QGDD1370 pKa = 4.1HH1371 pKa = 6.33VLANVVAQTGEE1382 pKa = 4.03EE1383 pKa = 4.6PICVPDD1389 pKa = 3.72SQLCTTHH1396 pKa = 6.96EE1397 pKa = 4.28LTPPPPLAATGGEE1410 pKa = 4.37VAASLLAAMMALLIGGGIFLAVSRR1434 pKa = 11.84RR1435 pKa = 11.84RR1436 pKa = 11.84RR1437 pKa = 11.84SVDD1440 pKa = 3.18QIGG1443 pKa = 3.11

Molecular weight: 148.89 kDa
Isoelectric point according different methods:





Protein with the highest isoelectric point:
>tr|A0A0U4F7G5|A0A0U4F7G5_9MICO DNA helicase OS=Microbacterium sp. XT11 OX=367477 GN=AB663_001250 PE=3 SV=1
MM1 pKa = 7.69SKK3 pKa = 9.0RR4 pKa = 11.84TFQPNNRR11 pKa = 11.84RR12 pKa = 11.84RR13 pKa = 11.84AKK15 pKa = 10.04KK16 pKa = 8.86HH17 pKa = 4.36GFRR20 pKa = 11.84ARR22 pKa = 11.84MRR24 pKa = 11.84TRR26 pKa = 11.84AGRR29 pKa = 11.84AILSARR35 pKa = 11.84RR36 pKa = 11.84AKK38 pKa = 10.67GRR40 pKa = 11.84VEE42 pKa = 4.42LSAA45 pKa = 5.19

Molecular weight: 5.25 kDa
Isoelectric point according different methods:





Peptides (in silico digests for buttom-up proteomics)

Below you can find in silico digests of the whole proteome with Trypsin, Chymotrypsin, Trypsin+LysC, LysN, ArgC proteases suitable for different mass spec machines.

Try
ESI
ChTry
ESI
ArgC
ESI
LysN
ESI
TryLysC
ESI

Try
MALDI
ChTry
MALDI
ArgC
MALDI
LysN
MALDI
TryLysC
MALDI

Try
LTQ
ChTry
LTQ
ArgC
LTQ
LysN
LTQ
TryLysC
LTQ

Try
MSlow
ChTry
MSlow
ArgC
MSlow
LysN
MSlow
TryLysC
MSlow

Try
MShigh
ChTry
MShigh
ArgC
MShigh
LysN
MShigh
TryLysC
MShigh

General Statistics

Number of major isoforms

Number of additional isoforms

Number of all proteins

Number of amino acids

Min. Seq. Length

Max. Seq. Length

Avg. Seq. Length

Avg. Mol. Weight

1927

0

1927

627981

38

2023

325.9

34.89

Amino acid frequency

Ala

Cys

Asp

Glu

Phe

Gly

His

Ile

Lys

Leu

13.756 ± 0.08

0.447 ± 0.012

6.452 ± 0.048

5.814 ± 0.05

3.16 ± 0.033

8.877 ± 0.052

2.001 ± 0.026

4.525 ± 0.038

1.927 ± 0.035

9.999 ± 0.062

Met

Asn

Gln

Pro

Arg

Ser

Thr

Val

Trp

Tyr

1.851 ± 0.023

1.876 ± 0.03

5.372 ± 0.038

2.716 ± 0.026

7.396 ± 0.071

5.505 ± 0.046

6.012 ± 0.049

8.836 ± 0.055

1.504 ± 0.022

1.974 ± 0.029
Note: For amino acid frequency statistics the error has been estimated with the bootstraping (x100) at the protein level

Most of the basic statistics you can see at this page can be downloaded from this CSV file

For dipeptide frequency statistics click here
See this proteome in: uniprot_link
Proteome-pI is available under Creative Commons Attribution-NoDerivs license, for more details see here

Reference: Kozlowski LP. Proteome-pI 2.0: Proteome Isoelectric Point Database Update. Nucleic Acids Res. 2021, doi: 10.1093/nar/gkab944 Contact: Lukasz P. Kozlowski