Circoviridae 15 LDMD-2013

Taxonomy: Viruses; Monodnaviria; Shotokuvirae; Cressdnaviricota; Arfiviricetes; Cirlivirales; Circoviridae; environmental samples

Average proteome isoelectric point is 7.36

Get precalculated fractions of proteins

Acidic
pI < 6.8
6.8-7.4
pI > 7.4
Basic
    
All



Virtual 2D-PAGE plot for 3 proteins (isoelectric point calculated using IPC2_protein)

Get csv file with sequences according to given criteria:
        -     Method 

     -  kDa    
                                                                                      

* You can choose from 21 different methods for calculating isoelectric point

Summary statistics related to proteome-wise predictions


    
Protein with the lowest isoelectric point:
>tr|S5TMX6|S5TMX6_9CIRC ATP-dependent helicase Rep OS=Circoviridae 15 LDMD-2013 OX=1379719 PE=3 SV=1
MM1 pKa = 7.49GFRR4 pKa = 11.84RR5 pKa = 11.84AFKK8 pKa = 10.26KK9 pKa = 9.87VKK11 pKa = 9.51QRR13 pKa = 11.84SGEE16 pKa = 4.02LLSGQEE22 pKa = 3.18IHH24 pKa = 5.35YY25 pKa = 7.34TXRR28 pKa = 11.84APSIMFDD35 pKa = 3.16PAVYY39 pKa = 10.68DD40 pKa = 3.69NAAWEE45 pKa = 4.3YY46 pKa = 10.24PKK48 pKa = 10.6GASLITVEE56 pKa = 4.35VIGVLMHH63 pKa = 7.81DD64 pKa = 4.05AGTNINISRR73 pKa = 11.84SEE75 pKa = 3.82TALDD79 pKa = 3.65VEE81 pKa = 5.3QVRR84 pKa = 11.84RR85 pKa = 11.84WKK87 pKa = 9.62WGLMKK92 pKa = 10.31PATKK96 pKa = 10.02RR97 pKa = 11.84ALALQPGVDD106 pKa = 4.01YY107 pKa = 11.65ANVTTPEE114 pKa = 3.84FADD117 pKa = 3.75PQEE120 pKa = 4.52GKK122 pKa = 9.14MEE124 pKa = 4.26TFEE127 pKa = 4.11EE128 pKa = 4.49

Molecular weight: 14.3 kDa
Isoelectric point according different methods:





Protein with the highest isoelectric point:
>tr|S5T699|S5T699_9CIRC Uncharacterized protein OS=Circoviridae 15 LDMD-2013 OX=1379719 PE=4 SV=1
MM1 pKa = 7.43PNARR5 pKa = 11.84RR6 pKa = 11.84KK7 pKa = 8.0YY8 pKa = 7.51TSKK11 pKa = 10.19SRR13 pKa = 11.84SSRR16 pKa = 11.84RR17 pKa = 11.84VRR19 pKa = 11.84RR20 pKa = 11.84TKK22 pKa = 9.94SARR25 pKa = 11.84KK26 pKa = 7.79RR27 pKa = 11.84TVRR30 pKa = 11.84YY31 pKa = 9.05KK32 pKa = 10.59RR33 pKa = 11.84KK34 pKa = 9.87ARR36 pKa = 11.84MLRR39 pKa = 11.84RR40 pKa = 11.84SRR42 pKa = 11.84RR43 pKa = 11.84GGRR46 pKa = 11.84RR47 pKa = 11.84SLMSYY52 pKa = 8.78MKK54 pKa = 10.44SQIIPGTMTSYY65 pKa = 11.38QDD67 pKa = 4.0LDD69 pKa = 3.47TSRR72 pKa = 11.84IEE74 pKa = 3.91VPKK77 pKa = 9.18GKK79 pKa = 10.33KK80 pKa = 9.41YY81 pKa = 10.3IAVFTVGNAPLMNTRR96 pKa = 11.84ATTSKK101 pKa = 10.7QLLNAVGTYY110 pKa = 10.5SVTTTDD116 pKa = 4.15FTQSALRR123 pKa = 11.84DD124 pKa = 3.56PRR126 pKa = 11.84VAFLEE131 pKa = 3.73QSIRR135 pKa = 11.84VLFRR139 pKa = 11.84NNTTAPRR146 pKa = 11.84LSKK149 pKa = 10.46LRR151 pKa = 11.84SINVSVRR158 pKa = 11.84KK159 pKa = 10.0RR160 pKa = 11.84ILL162 pKa = 3.28

Molecular weight: 18.73 kDa
Isoelectric point according different methods:





Peptides (in silico digests for buttom-up proteomics)

Below you can find in silico digests of the whole proteome with Trypsin, Chymotrypsin, Trypsin+LysC, LysN, ArgC proteases suitable for different mass spec machines.

Try
ESI
ChTry
ESI
ArgC
ESI
LysN
ESI
TryLysC
ESI

Try
MALDI
ChTry
MALDI
ArgC
MALDI
LysN
MALDI
TryLysC
MALDI

Try
LTQ
ChTry
LTQ
ArgC
LTQ
LysN
LTQ
TryLysC
LTQ

Try
MSlow
ChTry
MSlow
ArgC
MSlow
LysN
MSlow
TryLysC
MSlow

Try
MShigh
ChTry
MShigh
ArgC
MShigh
LysN
MShigh
TryLysC
MShigh

General Statistics

Number of major isoforms

Number of additional isoforms

Number of all proteins

Number of amino acids

Min. Seq. Length

Max. Seq. Length

Avg. Seq. Length

Avg. Mol. Weight

3

0

3

580

128

290

193.3

22.29

Amino acid frequency

Ala

Cys

Asp

Glu

Phe

Gly

His

Ile

Lys

Leu

6.897 ± 0.807

1.034 ± 0.615

5.345 ± 1.307

5.69 ± 1.712

3.793 ± 0.55

5.862 ± 0.833

1.552 ± 0.658

3.793 ± 0.395

6.379 ± 0.684

7.069 ± 0.208

Met

Asn

Gln

Pro

Arg

Ser

Thr

Val

Trp

Tyr

2.759 ± 0.605

3.793 ± 0.221

4.655 ± 0.369

3.103 ± 0.216

10.862 ± 2.47

6.724 ± 1.702

7.414 ± 1.207

6.897 ± 0.044

2.069 ± 0.849

4.138 ± 0.392
Note: For amino acid frequency statistics the error has been estimated with the bootstraping (x100) at the protein level

Most of the basic statistics you can see at this page can be downloaded from this CSV file

For dipeptide frequency statistics click here
See this proteome in: uniprot_link
Proteome-pI is available under Creative Commons Attribution-NoDerivs license, for more details see here

Reference: Kozlowski LP. Proteome-pI 2.0: Proteome Isoelectric Point Database Update. Nucleic Acids Res. 2021, doi: 10.1093/nar/gkab944 Contact: Lukasz P. Kozlowski