Hubei tombus-like virus 24

Taxonomy: Viruses; Riboviria; unclassified Riboviria; unclassified RNA viruses ShiM-2016

Average proteome isoelectric point is 7.34

Get precalculated fractions of proteins

Acidic
pI < 6.8
6.8-7.4
pI > 7.4
Basic
    
All



Virtual 2D-PAGE plot for 2 proteins (isoelectric point calculated using IPC2_protein)

Get csv file with sequences according to given criteria:
        -     Method 

     -  kDa    
                                                                                      

* You can choose from 21 different methods for calculating isoelectric point

Summary statistics related to proteome-wise predictions


    
Protein with the lowest isoelectric point:
>tr|A0A1L3KG46|A0A1L3KG46_9VIRU Uncharacterized protein OS=Hubei tombus-like virus 24 OX=1923271 PE=4 SV=1
MM1 pKa = 7.55FIKK4 pKa = 10.36HH5 pKa = 5.99EE6 pKa = 4.62KK7 pKa = 10.47GDD9 pKa = 4.12DD10 pKa = 3.74PEE12 pKa = 4.32STPRR16 pKa = 11.84GIQYY20 pKa = 10.63RR21 pKa = 11.84SFKK24 pKa = 8.17WTALFKK30 pKa = 10.57RR31 pKa = 11.84VLGPFEE37 pKa = 3.81MRR39 pKa = 11.84LWRR42 pKa = 11.84LGQDD46 pKa = 3.89FQPSPLAEE54 pKa = 3.82RR55 pKa = 11.84MFSKK59 pKa = 10.51NLNPRR64 pKa = 11.84AVAHH68 pKa = 5.9NLRR71 pKa = 11.84NGWLKK76 pKa = 10.52FADD79 pKa = 4.06PVADD83 pKa = 3.49LWDD86 pKa = 3.77VSRR89 pKa = 11.84MDD91 pKa = 3.15AHH93 pKa = 7.28LGRR96 pKa = 11.84LVRR99 pKa = 11.84EE100 pKa = 4.18MIEE103 pKa = 4.06FPTYY107 pKa = 11.03RR108 pKa = 11.84MGTYY112 pKa = 10.59GFDD115 pKa = 3.68DD116 pKa = 5.24FIGAMRR122 pKa = 11.84HH123 pKa = 5.3NICRR127 pKa = 11.84TKK129 pKa = 10.89NGIVYY134 pKa = 10.15EE135 pKa = 4.28MDD137 pKa = 2.99YY138 pKa = 11.03TMCSGEE144 pKa = 4.23ACTSAGDD151 pKa = 4.39SIVMAAVLDD160 pKa = 4.09FVYY163 pKa = 10.75RR164 pKa = 11.84DD165 pKa = 3.58IPHH168 pKa = 6.8HH169 pKa = 6.54KK170 pKa = 9.67LVCGDD175 pKa = 3.69DD176 pKa = 3.47CVVIRR181 pKa = 11.84EE182 pKa = 4.27RR183 pKa = 11.84GCVPDD188 pKa = 3.51SSVFAQCGLPVKK200 pKa = 10.52SDD202 pKa = 3.28VVDD205 pKa = 3.19QFEE208 pKa = 4.18RR209 pKa = 11.84VEE211 pKa = 4.55FCQSRR216 pKa = 11.84PVCVAGVWTMVRR228 pKa = 11.84NPDD231 pKa = 3.3RR232 pKa = 11.84VVNRR236 pKa = 11.84ALCTIKK242 pKa = 10.88NFGGEE247 pKa = 3.95VRR249 pKa = 11.84PYY251 pKa = 10.28QDD253 pKa = 2.37WLASVGVGEE262 pKa = 4.81LEE264 pKa = 4.27SGAGVPVVQTLALEE278 pKa = 4.12LMKK281 pKa = 10.69LGQPRR286 pKa = 11.84PHH288 pKa = 6.9FVRR291 pKa = 11.84EE292 pKa = 4.06YY293 pKa = 9.94LEE295 pKa = 3.88HH296 pKa = 7.38RR297 pKa = 11.84RR298 pKa = 11.84TLQQRR303 pKa = 11.84EE304 pKa = 4.2PLEE307 pKa = 4.21VTDD310 pKa = 4.05EE311 pKa = 4.32TRR313 pKa = 11.84HH314 pKa = 5.58SFWLAWGWTPEE325 pKa = 3.84EE326 pKa = 3.87QVRR329 pKa = 11.84FEE331 pKa = 4.11SLLLGGAEE339 pKa = 3.97FDD341 pKa = 4.36LVVV344 pKa = 3.48

Molecular weight: 39.37 kDa
Isoelectric point according different methods:





Protein with the highest isoelectric point:
>tr|A0A1L3KG46|A0A1L3KG46_9VIRU Uncharacterized protein OS=Hubei tombus-like virus 24 OX=1923271 PE=4 SV=1
MM1 pKa = 7.84GIGLSDD7 pKa = 3.85SSRR10 pKa = 11.84STPSLCATDD19 pKa = 5.29AGVCHH24 pKa = 6.34QSPQNPVARR33 pKa = 11.84ISGGRR38 pKa = 11.84DD39 pKa = 2.62EE40 pKa = 4.86RR41 pKa = 11.84RR42 pKa = 11.84PVNRR46 pKa = 11.84RR47 pKa = 11.84GVPVATQTSEE57 pKa = 4.27LPGHH61 pKa = 6.36ARR63 pKa = 11.84SVDD66 pKa = 3.41EE67 pKa = 4.13SHH69 pKa = 7.58PARR72 pKa = 11.84DD73 pKa = 3.73VVSKK77 pKa = 10.74SVAAIQDD84 pKa = 3.61RR85 pKa = 11.84VDD87 pKa = 3.31GLGAEE92 pKa = 4.56LSLPGWQRR100 pKa = 11.84AIPDD104 pKa = 3.94PRR106 pKa = 11.84KK107 pKa = 9.17DD108 pKa = 3.32TYY110 pKa = 11.57SEE112 pKa = 3.98LFSYY116 pKa = 10.61LRR118 pKa = 11.84RR119 pKa = 11.84QCGTSRR125 pKa = 11.84ISMLTLDD132 pKa = 3.89SMRR135 pKa = 11.84RR136 pKa = 11.84MGDD139 pKa = 2.24SWLARR144 pKa = 11.84NRR146 pKa = 11.84VWLSEE151 pKa = 4.03TARR154 pKa = 11.84TDD156 pKa = 3.06VLEE159 pKa = 4.02RR160 pKa = 11.84VIVDD164 pKa = 3.81LAQPQKK170 pKa = 11.09SDD172 pKa = 3.14TYY174 pKa = 10.65LASHH178 pKa = 5.3VTKK181 pKa = 9.73GTFYY185 pKa = 11.2SPISLGVGDD194 pKa = 3.86IKK196 pKa = 11.3SRR198 pKa = 11.84IAHH201 pKa = 5.84ANEE204 pKa = 3.28LRR206 pKa = 11.84AGRR209 pKa = 11.84RR210 pKa = 11.84WVPTKK215 pKa = 10.84NSWHH219 pKa = 6.46HH220 pKa = 5.81VLAALLFLAVALAHH234 pKa = 6.63PIGLVIALVGLGGVGYY250 pKa = 10.1SWVSTDD256 pKa = 2.81HH257 pKa = 6.73GLWEE261 pKa = 3.92QVIPEE266 pKa = 4.21KK267 pKa = 11.0

Molecular weight: 29.19 kDa
Isoelectric point according different methods:





Peptides (in silico digests for buttom-up proteomics)

Below you can find in silico digests of the whole proteome with Trypsin, Chymotrypsin, Trypsin+LysC, LysN, ArgC proteases suitable for different mass spec machines.

Try
ESI
ChTry
ESI
ArgC
ESI
LysN
ESI
TryLysC
ESI

Try
MALDI
ChTry
MALDI
ArgC
MALDI
LysN
MALDI
TryLysC
MALDI

Try
LTQ
ChTry
LTQ
ArgC
LTQ
LysN
LTQ
TryLysC
LTQ

Try
MSlow
ChTry
MSlow
ArgC
MSlow
LysN
MSlow
TryLysC
MSlow

Try
MShigh
ChTry
MShigh
ArgC
MShigh
LysN
MShigh
TryLysC
MShigh

General Statistics

Number of major isoforms

Number of additional isoforms

Number of all proteins

Number of amino acids

Min. Seq. Length

Max. Seq. Length

Avg. Seq. Length

Avg. Mol. Weight

2

0

2

611

267

344

305.5

34.28

Amino acid frequency

Ala

Cys

Asp

Glu

Phe

Gly

His

Ile

Lys

Leu

7.038 ± 1.063

2.128 ± 0.677

6.219 ± 0.153

5.565 ± 1.227

3.601 ± 1.67

7.856 ± 0.259

2.946 ± 0.286

3.601 ± 0.603

2.946 ± 0.219

9.002 ± 0.496

Met

Asn

Gln

Pro

Arg

Ser

Thr

Val

Trp

Tyr

2.619 ± 0.756

2.291 ± 0.282

5.565 ± 0.036

3.437 ± 0.045

9.002 ± 0.244

7.201 ± 2.215

4.583 ± 0.446

9.656 ± 0.45

2.619 ± 0.002

2.128 ± 0.172
Note: For amino acid frequency statistics the error has been estimated with the bootstraping (x100) at the protein level

Most of the basic statistics you can see at this page can be downloaded from this CSV file

For dipeptide frequency statistics click here
See this proteome in: uniprot_link
Proteome-pI is available under Creative Commons Attribution-NoDerivs license, for more details see here

Reference: Kozlowski LP. Proteome-pI 2.0: Proteome Isoelectric Point Database Update. Nucleic Acids Res. 2021, doi: 10.1093/nar/gkab944 Contact: Lukasz P. Kozlowski