Wuhan spider virus 8
Average proteome isoelectric point is 7.75
Get precalculated fractions of proteins
Acidic
pI < 6.8
6.8-7.4
pI > 7.4
Basic
All
Note: above files contain also dissociation constants (pKa)
Virtual 2D-PAGE plot for 4 proteins (isoelectric point calculated using IPC2_protein)
Get csv file with sequences according to given criteria:
* You can choose from 21 different methods for calculating isoelectric point
Summary statistics related to proteome-wise predictions
Protein with the lowest isoelectric point:
>tr|A0A1L3KGE8|A0A1L3KGE8_9VIRU RNA-directed RNA polymerase OS=Wuhan spider virus 8 OX=1923757 PE=4 SV=1
MM1 pKa = 6.88 FVKK4 pKa = 9.79 MAKK7 pKa = 9.8 TIGRR11 pKa = 11.84 QPKK14 pKa = 10.11 ASWKK18 pKa = 10.0 QMVASRR24 pKa = 11.84 MSKK27 pKa = 9.34 RR28 pKa = 11.84 RR29 pKa = 11.84 RR30 pKa = 11.84 RR31 pKa = 11.84 YY32 pKa = 9.69 ALGAQYY38 pKa = 10.83 YY39 pKa = 8.17 WEE41 pKa = 4.2 QGVTRR46 pKa = 11.84 QDD48 pKa = 2.86 AHH50 pKa = 7.36 LSAFLKK56 pKa = 10.99 LEE58 pKa = 4.33 FYY60 pKa = 10.75 KK61 pKa = 10.74 EE62 pKa = 3.77 EE63 pKa = 4.05 KK64 pKa = 10.44 LVEE67 pKa = 4.22 KK68 pKa = 10.23 EE69 pKa = 3.97 CRR71 pKa = 11.84 TIQHH75 pKa = 5.98 RR76 pKa = 11.84 RR77 pKa = 11.84 VAYY80 pKa = 10.33 NYY82 pKa = 11.17 ALLRR86 pKa = 11.84 WLCNIEE92 pKa = 3.55 HH93 pKa = 6.2 SMYY96 pKa = 10.98 SKK98 pKa = 10.69 LRR100 pKa = 11.84 NVDD103 pKa = 3.35 DD104 pKa = 3.87 SPRR107 pKa = 11.84 ILKK110 pKa = 9.56 SVPPPMRR117 pKa = 11.84 AFMVWRR123 pKa = 11.84 AWEE126 pKa = 4.19 SLGPTVRR133 pKa = 11.84 CYY135 pKa = 11.0 CLDD138 pKa = 3.14 HH139 pKa = 7.17 SRR141 pKa = 11.84 FDD143 pKa = 3.42 AHH145 pKa = 7.11 VVRR148 pKa = 11.84 DD149 pKa = 4.1 WLEE152 pKa = 3.96 MEE154 pKa = 3.83 HH155 pKa = 7.23 DD156 pKa = 4.55 FYY158 pKa = 12.01 KK159 pKa = 9.94 MCSEE163 pKa = 4.44 SRR165 pKa = 11.84 RR166 pKa = 11.84 EE167 pKa = 3.95 LAWLLRR173 pKa = 11.84 MQLRR177 pKa = 11.84 AHH179 pKa = 6.46 GRR181 pKa = 11.84 TAGGATYY188 pKa = 10.55 VQDD191 pKa = 4.27 GKK193 pKa = 10.99 RR194 pKa = 11.84 SSGDD198 pKa = 3.17 VNTASGNSADD208 pKa = 4.07 NDD210 pKa = 3.75 AMLLAHH216 pKa = 6.58 MPDD219 pKa = 3.59 NVCEE223 pKa = 4.22 IVAEE227 pKa = 4.31 VCVAAGFPDD236 pKa = 3.97 LASRR240 pKa = 11.84 ASDD243 pKa = 3.35 YY244 pKa = 11.14 RR245 pKa = 11.84 FAVALLLQLLEE256 pKa = 4.35 VEE258 pKa = 4.7 LTPKK262 pKa = 10.49 VGEE265 pKa = 4.22 EE266 pKa = 4.07 PPDD269 pKa = 3.89 PVEE272 pKa = 4.17 GTPLRR277 pKa = 11.84 VITYY281 pKa = 10.36 CDD283 pKa = 3.62 GDD285 pKa = 4.15 DD286 pKa = 4.66 GLVLSNFKK294 pKa = 10.87 LDD296 pKa = 3.59 FFFALFGFSTEE307 pKa = 3.83 ITVATSLEE315 pKa = 4.11 QIEE318 pKa = 4.79 FCQSKK323 pKa = 10.96 LIMTSGGPAFARR335 pKa = 11.84 DD336 pKa = 3.49 PAKK339 pKa = 10.29 VRR341 pKa = 11.84 NMVMHH346 pKa = 6.79 CRR348 pKa = 11.84 NLPYY352 pKa = 10.03 HH353 pKa = 5.29 QRR355 pKa = 11.84 EE356 pKa = 4.43 GVLKK360 pKa = 10.45 ASAFTEE366 pKa = 4.63 YY367 pKa = 10.16 IAQQGVWHH375 pKa = 6.16 NQMLAYY381 pKa = 9.4 KK382 pKa = 9.57 CYY384 pKa = 9.45 KK385 pKa = 10.02 ACGSASPIFLNKK397 pKa = 9.86 DD398 pKa = 3.56 LAWRR402 pKa = 11.84 CAALEE407 pKa = 3.99 PVEE410 pKa = 3.97 ITEE413 pKa = 4.67 PKK415 pKa = 8.89 WDD417 pKa = 5.01 PIAIASFEE425 pKa = 4.23 RR426 pKa = 11.84 AWQFTLEE433 pKa = 4.12 EE434 pKa = 4.42 ALFFDD439 pKa = 4.29 PVLRR443 pKa = 11.84 TATEE447 pKa = 4.28 GVVKK451 pKa = 10.64 KK452 pKa = 10.88 SSTLDD457 pKa = 3.51 DD458 pKa = 3.44 GTEE461 pKa = 3.86 QKK463 pKa = 10.98 EE464 pKa = 4.71 CFLPDD469 pKa = 3.77 HH470 pKa = 6.76 GWPDD474 pKa = 3.53 LVDD477 pKa = 3.55 PTVCWRR483 pKa = 11.84 CWAEE487 pKa = 4.12 QKK489 pKa = 10.68 PEE491 pKa = 3.56 AAAAEE496 pKa = 4.33 EE497 pKa = 4.17 TTRR500 pKa = 11.84 CCRR503 pKa = 11.84 HH504 pKa = 6.01 SCDD507 pKa = 4.39 SNCGGWGGDD516 pKa = 3.46 GG517 pKa = 3.63
Molecular weight: 58.76 kDa
Isoelectric point according different methods:
IPC2.protein.svr19 5.941
IPC2_protein 6.008
IPC_protein 6.046
Toseland 6.224
ProMoST 6.453
Dawson 6.376
Bjellqvist 6.376
Wikipedia 6.389
Rodwell 6.364
Grimsley 6.313
Solomon 6.376
Lehninger 6.376
Nozaki 6.664
DTASelect 6.81
Thurlkill 6.839
EMBOSS 6.81
Sillero 6.766
Patrickios 4.05
IPC_peptide 6.389
IPC2_peptide 6.693
IPC2.peptide.svr19 6.647
Protein with the highest isoelectric point:
>tr|A0A1L3KGE8|A0A1L3KGE8_9VIRU RNA-directed RNA polymerase OS=Wuhan spider virus 8 OX=1923757 PE=4 SV=1
MM1 pKa = 7.41 PAASFMEE8 pKa = 4.7 RR9 pKa = 11.84 IEE11 pKa = 4.12 YY12 pKa = 10.54 NITAAVTSAVLQSLGFVAPGTSSTPATSAAAAPPAPAKK50 pKa = 10.04 VVKK53 pKa = 9.36 TKK55 pKa = 9.12 KK56 pKa = 8.43 TPPKK60 pKa = 9.65 AAPVKK65 pKa = 10.17 AAAPPAPVKK74 pKa = 10.39 AAAPPAPEE82 pKa = 5.04 AEE84 pKa = 3.97 VDD86 pKa = 3.64 EE87 pKa = 4.96 RR88 pKa = 11.84 KK89 pKa = 8.14 QTAKK93 pKa = 9.86 QAKK96 pKa = 9.13 KK97 pKa = 10.05 AAKK100 pKa = 8.78 QAKK103 pKa = 8.92 KK104 pKa = 9.86 AAKK107 pKa = 10.43 GEE109 pKa = 4.27 LLNKK113 pKa = 9.4 AQQEE117 pKa = 4.38 QEE119 pKa = 4.06 KK120 pKa = 10.23 SASSHH125 pKa = 4.29 QEE127 pKa = 3.61 KK128 pKa = 10.79 PEE130 pKa = 4.02 TEE132 pKa = 4.02 ASAKK136 pKa = 8.82 TRR138 pKa = 11.84 LRR140 pKa = 11.84 RR141 pKa = 11.84 EE142 pKa = 3.63 QRR144 pKa = 11.84 KK145 pKa = 8.53 KK146 pKa = 9.97 QKK148 pKa = 10.11 KK149 pKa = 7.92 SKK151 pKa = 10.54 KK152 pKa = 8.15 PAKK155 pKa = 10.06 QEE157 pKa = 3.7 GFTPAKK163 pKa = 9.33 PDD165 pKa = 3.31 KK166 pKa = 10.39 KK167 pKa = 11.17 GKK169 pKa = 9.25 GKK171 pKa = 10.49 KK172 pKa = 9.4 KK173 pKa = 9.5 ATPEE177 pKa = 3.83 TAGFVPLGEE186 pKa = 4.44 SGAKK190 pKa = 9.48 RR191 pKa = 11.84 EE192 pKa = 4.21 KK193 pKa = 10.95 KK194 pKa = 9.84 FGKK197 pKa = 10.33 ADD199 pKa = 3.32 ATHH202 pKa = 6.51 YY203 pKa = 11.16 KK204 pKa = 9.71 MGWTKK209 pKa = 10.88 EE210 pKa = 4.12 EE211 pKa = 3.78 QTKK214 pKa = 10.6 ASSAFRR220 pKa = 11.84 CDD222 pKa = 3.67 LACVRR227 pKa = 11.84 CNYY230 pKa = 8.59 ATGNEE235 pKa = 3.81 ICNNCRR241 pKa = 11.84 KK242 pKa = 9.69 FIKK245 pKa = 10.15 LVCCSVEE252 pKa = 4.25 KK253 pKa = 10.65 PNEE256 pKa = 3.8 AHH258 pKa = 6.55 AKK260 pKa = 7.68 WVAGGKK266 pKa = 9.92 LGEE269 pKa = 4.76 EE270 pKa = 4.45 PPATYY275 pKa = 9.9 IGAAEE280 pKa = 3.87 RR281 pKa = 11.84 RR282 pKa = 11.84 VEE284 pKa = 4.04 RR285 pKa = 11.84 SRR287 pKa = 11.84 GRR289 pKa = 11.84 MRR291 pKa = 11.84 VVCSGCDD298 pKa = 3.35 TPIVDD303 pKa = 4.24 WPRR306 pKa = 11.84 FMLGWQHH313 pKa = 6.99 AEE315 pKa = 3.92 APSVRR320 pKa = 11.84 IIMCKK325 pKa = 9.92 RR326 pKa = 11.84 KK327 pKa = 9.9 ACRR330 pKa = 11.84 EE331 pKa = 3.73 PAAAGWLNPGSSFSPKK347 pKa = 9.23 QVEE350 pKa = 4.41 EE351 pKa = 4.96 GPVIATADD359 pKa = 3.78 EE360 pKa = 4.03 LRR362 pKa = 11.84 GISKK366 pKa = 10.42 KK367 pKa = 10.51 EE368 pKa = 3.44 EE369 pKa = 4.48 SKK371 pKa = 10.45 PHH373 pKa = 5.11 TRR375 pKa = 11.84 GNPRR379 pKa = 11.84 LQDD382 pKa = 3.69 FLSEE386 pKa = 4.1 NQFAALEE393 pKa = 4.16 EE394 pKa = 4.2 ASAAAGAKK402 pKa = 9.84 GPRR405 pKa = 11.84 VTPKK409 pKa = 9.84 RR410 pKa = 11.84 PKK412 pKa = 9.75 KK413 pKa = 10.14 ARR415 pKa = 11.84 QSVEE419 pKa = 4.0 AGSQTTEE426 pKa = 3.65 PDD428 pKa = 2.95 LRR430 pKa = 11.84 NYY432 pKa = 10.13 ISLDD436 pKa = 3.23 AVRR439 pKa = 11.84 TAIEE443 pKa = 4.13 TGDD446 pKa = 3.77 LEE448 pKa = 4.62 RR449 pKa = 11.84 LYY451 pKa = 11.32 LEE453 pKa = 4.85 AATHH457 pKa = 6.27 AALSAAKK464 pKa = 10.16 ASVDD468 pKa = 3.09 AGTQTEE474 pKa = 4.81 VPHH477 pKa = 5.93 KK478 pKa = 10.19 HH479 pKa = 6.21 SVACGPDD486 pKa = 3.5 AEE488 pKa = 4.53 QSEE491 pKa = 4.92 TPAAQDD497 pKa = 3.43 SVASDD502 pKa = 3.63 VAPSPSTSFAQAVRR516 pKa = 11.84 GATASTSAAPQDD528 pKa = 3.64 KK529 pKa = 10.8 GVGQTPEE536 pKa = 3.88 KK537 pKa = 9.91 QQPKK541 pKa = 9.68 ASKK544 pKa = 10.32 SSKK547 pKa = 9.37 PWPKK551 pKa = 10.64 DD552 pKa = 3.1 KK553 pKa = 10.75 PVALAVGMSTLDD565 pKa = 3.22 WVEE568 pKa = 5.35 DD569 pKa = 4.72 GIQEE573 pKa = 4.76 LKK575 pKa = 11.13 GKK577 pKa = 9.68 FDD579 pKa = 3.93 KK580 pKa = 11.07 PHH582 pKa = 5.94 GPKK585 pKa = 10.28 ARR587 pKa = 11.84 GVLLFSDD594 pKa = 4.3 VPRR597 pKa = 11.84 TYY599 pKa = 10.53 SYY601 pKa = 11.48 SGVKK605 pKa = 10.45 LSPRR609 pKa = 11.84 EE610 pKa = 3.86 KK611 pKa = 10.27 LPWMVSLEE619 pKa = 4.0 RR620 pKa = 11.84 LALKK624 pKa = 9.89 YY625 pKa = 10.57 LKK627 pKa = 10.54 EE628 pKa = 4.08 EE629 pKa = 4.35 GGLDD633 pKa = 3.78 VKK635 pKa = 10.29 PTDD638 pKa = 3.61 FGMLVVNDD646 pKa = 3.76 YY647 pKa = 11.45 GKK649 pKa = 10.35 DD650 pKa = 3.32 QGIPEE655 pKa = 4.5 HH656 pKa = 7.0 SDD658 pKa = 2.99 NEE660 pKa = 3.98 RR661 pKa = 11.84 DD662 pKa = 3.28 IDD664 pKa = 3.51 QDD666 pKa = 3.57 RR667 pKa = 11.84 PIISLSVGGKK677 pKa = 9.73 RR678 pKa = 11.84 LMTFTDD684 pKa = 3.77 LKK686 pKa = 10.78 GEE688 pKa = 4.24 VVEE691 pKa = 4.52 EE692 pKa = 4.27 VPLASWDD699 pKa = 3.94 LVVMPPGSQKK709 pKa = 10.54 LFHH712 pKa = 6.84 HH713 pKa = 6.35 KK714 pKa = 10.35 VDD716 pKa = 3.92 GEE718 pKa = 4.27 GGPRR722 pKa = 11.84 TNFTWRR728 pKa = 11.84 AWKK731 pKa = 10.3 SAGRR735 pKa = 11.84 PHH737 pKa = 7.01 PRR739 pKa = 3.24
Molecular weight: 79.68 kDa
Isoelectric point according different methods:
IPC2.protein.svr19 8.788
IPC2_protein 8.756
IPC_protein 8.595
Toseland 9.75
ProMoST 9.297
Dawson 9.882
Bjellqvist 9.502
Wikipedia 9.97
Rodwell 10.526
Grimsley 9.897
Solomon 9.897
Lehninger 9.882
Nozaki 9.823
DTASelect 9.458
Thurlkill 9.765
EMBOSS 10.131
Sillero 9.823
Patrickios 10.16
IPC_peptide 9.897
IPC2_peptide 7.966
IPC2.peptide.svr19 7.688
Peptides (in silico digests for buttom-up proteomics)
Below you can find
in silico digests of the whole proteome with Trypsin, Chymotrypsin, Trypsin+LysC, LysN, ArgC proteases suitable for different mass spec machines.
Try ESI
ChTry ESI
ArgC ESI
LysN ESI
TryLysC ESI
Try MALDI
ChTry MALDI
ArgC MALDI
LysN MALDI
TryLysC MALDI
Try LTQ
ChTry LTQ
ArgC LTQ
LysN LTQ
TryLysC LTQ
Try MSlow
ChTry MSlow
ArgC MSlow
LysN MSlow
TryLysC MSlow
Try MShigh
ChTry MShigh
ArgC MShigh
LysN MShigh
TryLysC MShigh
General Statistics
Number of major isoforms
Number of additional isoforms
Number of all proteins
Number of amino acids
Min. Seq. Length
Max. Seq. Length
Avg. Seq. Length
Avg. Mol. Weight
4
0
4
1926
257
739
481.5
53.02
Amino acid frequency
Ala
Cys
Asp
Glu
Phe
Gly
His
Ile
Lys
Leu
11.89 ± 0.778
2.077 ± 0.556
4.673 ± 0.404
6.646 ± 0.857
3.271 ± 0.511
6.802 ± 0.377
2.025 ± 0.163
2.7 ± 0.208
6.854 ± 1.775
7.788 ± 1.085
Met
Asn
Gln
Pro
Arg
Ser
Thr
Val
Trp
Tyr
2.388 ± 0.399
2.752 ± 0.584
7.009 ± 0.797
3.946 ± 0.255
6.438 ± 0.421
6.75 ± 0.459
5.659 ± 0.663
5.919 ± 0.388
2.336 ± 0.387
2.077 ± 0.57
Note: For amino acid frequency statistics the error has been estimated with the bootstraping (x100) at the protein level
Most of the basic statistics you can see at this page can be downloaded from this CSV file
For dipeptide frequency statistics click here