Wuhan spider virus 8

Taxonomy: Viruses; Riboviria; unclassified Riboviria; unclassified RNA viruses ShiM-2016

Average proteome isoelectric point is 7.75

Get precalculated fractions of proteins

Acidic
pI < 6.8
6.8-7.4
pI > 7.4
Basic
    
All



Virtual 2D-PAGE plot for 4 proteins (isoelectric point calculated using IPC2_protein)

Get csv file with sequences according to given criteria:
        -     Method 

     -  kDa    
                                                                                      

* You can choose from 21 different methods for calculating isoelectric point

Summary statistics related to proteome-wise predictions


    

Protein with the lowest isoelectric point:
>tr|A0A1L3KGE8|A0A1L3KGE8_9VIRU RNA-directed RNA polymerase OS=Wuhan spider virus 8 OX=1923757 PE=4 SV=1
MM1 pKa = 6.88FVKK4 pKa = 9.79MAKK7 pKa = 9.8TIGRR11 pKa = 11.84QPKK14 pKa = 10.11ASWKK18 pKa = 10.0QMVASRR24 pKa = 11.84MSKK27 pKa = 9.34RR28 pKa = 11.84RR29 pKa = 11.84RR30 pKa = 11.84RR31 pKa = 11.84YY32 pKa = 9.69ALGAQYY38 pKa = 10.83YY39 pKa = 8.17WEE41 pKa = 4.2QGVTRR46 pKa = 11.84QDD48 pKa = 2.86AHH50 pKa = 7.36LSAFLKK56 pKa = 10.99LEE58 pKa = 4.33FYY60 pKa = 10.75KK61 pKa = 10.74EE62 pKa = 3.77EE63 pKa = 4.05KK64 pKa = 10.44LVEE67 pKa = 4.22KK68 pKa = 10.23EE69 pKa = 3.97CRR71 pKa = 11.84TIQHH75 pKa = 5.98RR76 pKa = 11.84RR77 pKa = 11.84VAYY80 pKa = 10.33NYY82 pKa = 11.17ALLRR86 pKa = 11.84WLCNIEE92 pKa = 3.55HH93 pKa = 6.2SMYY96 pKa = 10.98SKK98 pKa = 10.69LRR100 pKa = 11.84NVDD103 pKa = 3.35DD104 pKa = 3.87SPRR107 pKa = 11.84ILKK110 pKa = 9.56SVPPPMRR117 pKa = 11.84AFMVWRR123 pKa = 11.84AWEE126 pKa = 4.19SLGPTVRR133 pKa = 11.84CYY135 pKa = 11.0CLDD138 pKa = 3.14HH139 pKa = 7.17SRR141 pKa = 11.84FDD143 pKa = 3.42AHH145 pKa = 7.11VVRR148 pKa = 11.84DD149 pKa = 4.1WLEE152 pKa = 3.96MEE154 pKa = 3.83HH155 pKa = 7.23DD156 pKa = 4.55FYY158 pKa = 12.01KK159 pKa = 9.94MCSEE163 pKa = 4.44SRR165 pKa = 11.84RR166 pKa = 11.84EE167 pKa = 3.95LAWLLRR173 pKa = 11.84MQLRR177 pKa = 11.84AHH179 pKa = 6.46GRR181 pKa = 11.84TAGGATYY188 pKa = 10.55VQDD191 pKa = 4.27GKK193 pKa = 10.99RR194 pKa = 11.84SSGDD198 pKa = 3.17VNTASGNSADD208 pKa = 4.07NDD210 pKa = 3.75AMLLAHH216 pKa = 6.58MPDD219 pKa = 3.59NVCEE223 pKa = 4.22IVAEE227 pKa = 4.31VCVAAGFPDD236 pKa = 3.97LASRR240 pKa = 11.84ASDD243 pKa = 3.35YY244 pKa = 11.14RR245 pKa = 11.84FAVALLLQLLEE256 pKa = 4.35VEE258 pKa = 4.7LTPKK262 pKa = 10.49VGEE265 pKa = 4.22EE266 pKa = 4.07PPDD269 pKa = 3.89PVEE272 pKa = 4.17GTPLRR277 pKa = 11.84VITYY281 pKa = 10.36CDD283 pKa = 3.62GDD285 pKa = 4.15DD286 pKa = 4.66GLVLSNFKK294 pKa = 10.87LDD296 pKa = 3.59FFFALFGFSTEE307 pKa = 3.83ITVATSLEE315 pKa = 4.11QIEE318 pKa = 4.79FCQSKK323 pKa = 10.96LIMTSGGPAFARR335 pKa = 11.84DD336 pKa = 3.49PAKK339 pKa = 10.29VRR341 pKa = 11.84NMVMHH346 pKa = 6.79CRR348 pKa = 11.84NLPYY352 pKa = 10.03HH353 pKa = 5.29QRR355 pKa = 11.84EE356 pKa = 4.43GVLKK360 pKa = 10.45ASAFTEE366 pKa = 4.63YY367 pKa = 10.16IAQQGVWHH375 pKa = 6.16NQMLAYY381 pKa = 9.4KK382 pKa = 9.57CYY384 pKa = 9.45KK385 pKa = 10.02ACGSASPIFLNKK397 pKa = 9.86DD398 pKa = 3.56LAWRR402 pKa = 11.84CAALEE407 pKa = 3.99PVEE410 pKa = 3.97ITEE413 pKa = 4.67PKK415 pKa = 8.89WDD417 pKa = 5.01PIAIASFEE425 pKa = 4.23RR426 pKa = 11.84AWQFTLEE433 pKa = 4.12EE434 pKa = 4.42ALFFDD439 pKa = 4.29PVLRR443 pKa = 11.84TATEE447 pKa = 4.28GVVKK451 pKa = 10.64KK452 pKa = 10.88SSTLDD457 pKa = 3.51DD458 pKa = 3.44GTEE461 pKa = 3.86QKK463 pKa = 10.98EE464 pKa = 4.71CFLPDD469 pKa = 3.77HH470 pKa = 6.76GWPDD474 pKa = 3.53LVDD477 pKa = 3.55PTVCWRR483 pKa = 11.84CWAEE487 pKa = 4.12QKK489 pKa = 10.68PEE491 pKa = 3.56AAAAEE496 pKa = 4.33EE497 pKa = 4.17TTRR500 pKa = 11.84CCRR503 pKa = 11.84HH504 pKa = 6.01SCDD507 pKa = 4.39SNCGGWGGDD516 pKa = 3.46GG517 pKa = 3.63

Molecular weight:
58.76 kDa
Isoelectric point according different methods:






Protein with the highest isoelectric point:
>tr|A0A1L3KGE8|A0A1L3KGE8_9VIRU RNA-directed RNA polymerase OS=Wuhan spider virus 8 OX=1923757 PE=4 SV=1
MM1 pKa = 7.41PAASFMEE8 pKa = 4.7RR9 pKa = 11.84IEE11 pKa = 4.12YY12 pKa = 10.54NITAAVTSAVLQSLGFVAPGTSSTPATSAAAAPPAPAKK50 pKa = 10.04VVKK53 pKa = 9.36TKK55 pKa = 9.12KK56 pKa = 8.43TPPKK60 pKa = 9.65AAPVKK65 pKa = 10.17AAAPPAPVKK74 pKa = 10.39AAAPPAPEE82 pKa = 5.04AEE84 pKa = 3.97VDD86 pKa = 3.64EE87 pKa = 4.96RR88 pKa = 11.84KK89 pKa = 8.14QTAKK93 pKa = 9.86QAKK96 pKa = 9.13KK97 pKa = 10.05AAKK100 pKa = 8.78QAKK103 pKa = 8.92KK104 pKa = 9.86AAKK107 pKa = 10.43GEE109 pKa = 4.27LLNKK113 pKa = 9.4AQQEE117 pKa = 4.38QEE119 pKa = 4.06KK120 pKa = 10.23SASSHH125 pKa = 4.29QEE127 pKa = 3.61KK128 pKa = 10.79PEE130 pKa = 4.02TEE132 pKa = 4.02ASAKK136 pKa = 8.82TRR138 pKa = 11.84LRR140 pKa = 11.84RR141 pKa = 11.84EE142 pKa = 3.63QRR144 pKa = 11.84KK145 pKa = 8.53KK146 pKa = 9.97QKK148 pKa = 10.11KK149 pKa = 7.92SKK151 pKa = 10.54KK152 pKa = 8.15PAKK155 pKa = 10.06QEE157 pKa = 3.7GFTPAKK163 pKa = 9.33PDD165 pKa = 3.31KK166 pKa = 10.39KK167 pKa = 11.17GKK169 pKa = 9.25GKK171 pKa = 10.49KK172 pKa = 9.4KK173 pKa = 9.5ATPEE177 pKa = 3.83TAGFVPLGEE186 pKa = 4.44SGAKK190 pKa = 9.48RR191 pKa = 11.84EE192 pKa = 4.21KK193 pKa = 10.95KK194 pKa = 9.84FGKK197 pKa = 10.33ADD199 pKa = 3.32ATHH202 pKa = 6.51YY203 pKa = 11.16KK204 pKa = 9.71MGWTKK209 pKa = 10.88EE210 pKa = 4.12EE211 pKa = 3.78QTKK214 pKa = 10.6ASSAFRR220 pKa = 11.84CDD222 pKa = 3.67LACVRR227 pKa = 11.84CNYY230 pKa = 8.59ATGNEE235 pKa = 3.81ICNNCRR241 pKa = 11.84KK242 pKa = 9.69FIKK245 pKa = 10.15LVCCSVEE252 pKa = 4.25KK253 pKa = 10.65PNEE256 pKa = 3.8AHH258 pKa = 6.55AKK260 pKa = 7.68WVAGGKK266 pKa = 9.92LGEE269 pKa = 4.76EE270 pKa = 4.45PPATYY275 pKa = 9.9IGAAEE280 pKa = 3.87RR281 pKa = 11.84RR282 pKa = 11.84VEE284 pKa = 4.04RR285 pKa = 11.84SRR287 pKa = 11.84GRR289 pKa = 11.84MRR291 pKa = 11.84VVCSGCDD298 pKa = 3.35TPIVDD303 pKa = 4.24WPRR306 pKa = 11.84FMLGWQHH313 pKa = 6.99AEE315 pKa = 3.92APSVRR320 pKa = 11.84IIMCKK325 pKa = 9.92RR326 pKa = 11.84KK327 pKa = 9.9ACRR330 pKa = 11.84EE331 pKa = 3.73PAAAGWLNPGSSFSPKK347 pKa = 9.23QVEE350 pKa = 4.41EE351 pKa = 4.96GPVIATADD359 pKa = 3.78EE360 pKa = 4.03LRR362 pKa = 11.84GISKK366 pKa = 10.42KK367 pKa = 10.51EE368 pKa = 3.44EE369 pKa = 4.48SKK371 pKa = 10.45PHH373 pKa = 5.11TRR375 pKa = 11.84GNPRR379 pKa = 11.84LQDD382 pKa = 3.69FLSEE386 pKa = 4.1NQFAALEE393 pKa = 4.16EE394 pKa = 4.2ASAAAGAKK402 pKa = 9.84GPRR405 pKa = 11.84VTPKK409 pKa = 9.84RR410 pKa = 11.84PKK412 pKa = 9.75KK413 pKa = 10.14ARR415 pKa = 11.84QSVEE419 pKa = 4.0AGSQTTEE426 pKa = 3.65PDD428 pKa = 2.95LRR430 pKa = 11.84NYY432 pKa = 10.13ISLDD436 pKa = 3.23AVRR439 pKa = 11.84TAIEE443 pKa = 4.13TGDD446 pKa = 3.77LEE448 pKa = 4.62RR449 pKa = 11.84LYY451 pKa = 11.32LEE453 pKa = 4.85AATHH457 pKa = 6.27AALSAAKK464 pKa = 10.16ASVDD468 pKa = 3.09AGTQTEE474 pKa = 4.81VPHH477 pKa = 5.93KK478 pKa = 10.19HH479 pKa = 6.21SVACGPDD486 pKa = 3.5AEE488 pKa = 4.53QSEE491 pKa = 4.92TPAAQDD497 pKa = 3.43SVASDD502 pKa = 3.63VAPSPSTSFAQAVRR516 pKa = 11.84GATASTSAAPQDD528 pKa = 3.64KK529 pKa = 10.8GVGQTPEE536 pKa = 3.88KK537 pKa = 9.91QQPKK541 pKa = 9.68ASKK544 pKa = 10.32SSKK547 pKa = 9.37PWPKK551 pKa = 10.64DD552 pKa = 3.1KK553 pKa = 10.75PVALAVGMSTLDD565 pKa = 3.22WVEE568 pKa = 5.35DD569 pKa = 4.72GIQEE573 pKa = 4.76LKK575 pKa = 11.13GKK577 pKa = 9.68FDD579 pKa = 3.93KK580 pKa = 11.07PHH582 pKa = 5.94GPKK585 pKa = 10.28ARR587 pKa = 11.84GVLLFSDD594 pKa = 4.3VPRR597 pKa = 11.84TYY599 pKa = 10.53SYY601 pKa = 11.48SGVKK605 pKa = 10.45LSPRR609 pKa = 11.84EE610 pKa = 3.86KK611 pKa = 10.27LPWMVSLEE619 pKa = 4.0RR620 pKa = 11.84LALKK624 pKa = 9.89YY625 pKa = 10.57LKK627 pKa = 10.54EE628 pKa = 4.08EE629 pKa = 4.35GGLDD633 pKa = 3.78VKK635 pKa = 10.29PTDD638 pKa = 3.61FGMLVVNDD646 pKa = 3.76YY647 pKa = 11.45GKK649 pKa = 10.35DD650 pKa = 3.32QGIPEE655 pKa = 4.5HH656 pKa = 7.0SDD658 pKa = 2.99NEE660 pKa = 3.98RR661 pKa = 11.84DD662 pKa = 3.28IDD664 pKa = 3.51QDD666 pKa = 3.57RR667 pKa = 11.84PIISLSVGGKK677 pKa = 9.73RR678 pKa = 11.84LMTFTDD684 pKa = 3.77LKK686 pKa = 10.78GEE688 pKa = 4.24VVEE691 pKa = 4.52EE692 pKa = 4.27VPLASWDD699 pKa = 3.94LVVMPPGSQKK709 pKa = 10.54LFHH712 pKa = 6.84HH713 pKa = 6.35KK714 pKa = 10.35VDD716 pKa = 3.92GEE718 pKa = 4.27GGPRR722 pKa = 11.84TNFTWRR728 pKa = 11.84AWKK731 pKa = 10.3SAGRR735 pKa = 11.84PHH737 pKa = 7.01PRR739 pKa = 3.24

Molecular weight:
79.68 kDa
Isoelectric point according different methods:






Peptides (in silico digests for buttom-up proteomics)

Below you can find in silico digests of the whole proteome with Trypsin, Chymotrypsin, Trypsin+LysC, LysN, ArgC proteases suitable for different mass spec machines.

Try
ESI
ChTry
ESI
ArgC
ESI
LysN
ESI
TryLysC
ESI

Try
MALDI
ChTry
MALDI
ArgC
MALDI
LysN
MALDI
TryLysC
MALDI

Try
LTQ
ChTry
LTQ
ArgC
LTQ
LysN
LTQ
TryLysC
LTQ

Try
MSlow
ChTry
MSlow
ArgC
MSlow
LysN
MSlow
TryLysC
MSlow

Try
MShigh
ChTry
MShigh
ArgC
MShigh
LysN
MShigh
TryLysC
MShigh

General Statistics

Number of major isoforms

Number of additional isoforms

Number of all proteins

Number of amino acids

Min. Seq. Length

Max. Seq. Length

Avg. Seq. Length

Avg. Mol. Weight

4

0

4

1926

257

739

481.5

53.02

Amino acid frequency

Ala

Cys

Asp

Glu

Phe

Gly

His

Ile

Lys

Leu

11.89 ± 0.778

2.077 ± 0.556

4.673 ± 0.404

6.646 ± 0.857

3.271 ± 0.511

6.802 ± 0.377

2.025 ± 0.163

2.7 ± 0.208

6.854 ± 1.775

7.788 ± 1.085

Met

Asn

Gln

Pro

Arg

Ser

Thr

Val

Trp

Tyr

2.388 ± 0.399

2.752 ± 0.584

7.009 ± 0.797

3.946 ± 0.255

6.438 ± 0.421

6.75 ± 0.459

5.659 ± 0.663

5.919 ± 0.388

2.336 ± 0.387

2.077 ± 0.57

Note: For amino acid frequency statistics the error has been estimated with the bootstraping (x100) at the protein level

Most of the basic statistics you can see at this page can be downloaded from this CSV file

For dipeptide frequency statistics click here
See this proteome in: uniprot_link
Proteome-pI is available under Creative Commons Attribution-NoDerivs license, for more details see here

Reference: Kozlowski LP. Proteome-pI 2.0: Proteome Isoelectric Point Database Update. Nucleic Acids Res. 2021, doi: 10.1093/nar/gkab944 Contact: Lukasz P. Kozlowski