Enteroscipio rubneri

Taxonomy: cellular organisms; Bacteria; Terrabacteria group; Actinobacteria; Coriobacteriia; Eggerthellales; Eggerthellaceae; Enteroscipio

Average proteome isoelectric point is 5.76

Get precalculated fractions of proteins

Acidic
pI < 6.8
6.8-7.4
pI > 7.4
Basic
    
All



Virtual 2D-PAGE plot for 2260 proteins (isoelectric point calculated using IPC2_protein)

Get csv file with sequences according to given criteria:
        -     Method 

     -  kDa    
                                                                                      

* You can choose from 21 different methods for calculating isoelectric point

Summary statistics related to proteome-wise predictions


    
Protein with the lowest isoelectric point:
>tr|A0A2K2U934|A0A2K2U934_9ACTN Ribonuclease 3 OS=Enteroscipio rubneri OX=2070686 GN=rnc PE=3 SV=1
MM1 pKa = 8.12DD2 pKa = 4.27PTLRR6 pKa = 11.84IAVPPEE12 pKa = 3.78LFAPAEE18 pKa = 4.05SSRR21 pKa = 11.84FEE23 pKa = 4.18GALEE27 pKa = 4.21LPSLQAGPDD36 pKa = 3.54EE37 pKa = 4.67YY38 pKa = 11.58SFPDD42 pKa = 3.52PLAWHH47 pKa = 6.12VEE49 pKa = 3.65ISNTGDD55 pKa = 3.26ALLVTGTVSGEE66 pKa = 4.28AIGSCARR73 pKa = 11.84CLDD76 pKa = 3.57GFRR79 pKa = 11.84FDD81 pKa = 5.3LEE83 pKa = 4.43GEE85 pKa = 3.93IEE87 pKa = 4.76GYY89 pKa = 10.51YY90 pKa = 10.92LLDD93 pKa = 3.87EE94 pKa = 4.72SRR96 pKa = 11.84SAPEE100 pKa = 5.73DD101 pKa = 3.42MDD103 pKa = 6.27DD104 pKa = 5.94DD105 pKa = 4.29EE106 pKa = 7.04FDD108 pKa = 4.03VLPEE112 pKa = 3.78DD113 pKa = 4.24HH114 pKa = 7.38VIDD117 pKa = 5.15LEE119 pKa = 4.21PLIKK123 pKa = 10.33AALILEE129 pKa = 4.57FPLVPLCDD137 pKa = 3.98EE138 pKa = 4.24EE139 pKa = 5.74CKK141 pKa = 10.82GLCAVCGANLNEE153 pKa = 4.97GPCEE157 pKa = 3.92CAPTEE162 pKa = 4.18GANDD166 pKa = 3.9GPANPFSVLKK176 pKa = 10.53DD177 pKa = 3.87FPFDD181 pKa = 3.86DD182 pKa = 3.94RR183 pKa = 5.49

Molecular weight: 19.75 kDa
Isoelectric point according different methods:





Protein with the highest isoelectric point:
>tr|A0A2K2UC13|A0A2K2UC13_9ACTN 1-acyl-sn-glycerol-3-phosphate acyltransferase OS=Enteroscipio rubneri OX=2070686 GN=C2L71_06175 PE=4 SV=1
MM1 pKa = 7.11TRR3 pKa = 11.84GGRR6 pKa = 11.84HH7 pKa = 5.23RR8 pKa = 11.84AWTTRR13 pKa = 11.84EE14 pKa = 3.64VAFLIDD20 pKa = 3.29AAGRR24 pKa = 11.84LPLRR28 pKa = 11.84TICKK32 pKa = 9.58RR33 pKa = 11.84LGRR36 pKa = 11.84SEE38 pKa = 4.34SAVEE42 pKa = 4.36SMASKK47 pKa = 10.63LNRR50 pKa = 11.84SGEE53 pKa = 4.15HH54 pKa = 5.69LTLKK58 pKa = 10.02CWSSRR63 pKa = 11.84LVWCNEE69 pKa = 3.48CATWRR74 pKa = 11.84TGIDD78 pKa = 3.77DD79 pKa = 3.33KK80 pKa = 11.28TGRR83 pKa = 11.84CRR85 pKa = 11.84VCTMRR90 pKa = 11.84EE91 pKa = 3.77QLRR94 pKa = 11.84GRR96 pKa = 11.84EE97 pKa = 4.13DD98 pKa = 3.09ACSRR102 pKa = 11.84EE103 pKa = 4.1LAIMTPEE110 pKa = 3.48QRR112 pKa = 11.84LVYY115 pKa = 10.15EE116 pKa = 3.89RR117 pKa = 11.84TEE119 pKa = 3.75AQRR122 pKa = 11.84RR123 pKa = 11.84TRR125 pKa = 11.84KK126 pKa = 9.9ALEE129 pKa = 4.17RR130 pKa = 11.84PQKK133 pKa = 10.44RR134 pKa = 11.84GSCPMSRR141 pKa = 11.84YY142 pKa = 8.89EE143 pKa = 3.81RR144 pKa = 11.84SKK146 pKa = 11.15AEE148 pKa = 3.65EE149 pKa = 4.4RR150 pKa = 11.84YY151 pKa = 10.63LIDD154 pKa = 4.06LEE156 pKa = 4.02EE157 pKa = 4.09WEE159 pKa = 4.38YY160 pKa = 10.02RR161 pKa = 11.84TLRR164 pKa = 11.84LRR166 pKa = 11.84YY167 pKa = 9.27DD168 pKa = 3.24AAKK171 pKa = 9.57TRR173 pKa = 11.84LRR175 pKa = 11.84RR176 pKa = 11.84MRR178 pKa = 11.84EE179 pKa = 3.58KK180 pKa = 10.13TGRR183 pKa = 11.84NPRR186 pKa = 11.84KK187 pKa = 9.61GRR189 pKa = 11.84PPSPSEE195 pKa = 3.82SRR197 pKa = 11.84II198 pKa = 3.62

Molecular weight: 23.38 kDa
Isoelectric point according different methods:





Peptides (in silico digests for buttom-up proteomics)

Below you can find in silico digests of the whole proteome with Trypsin, Chymotrypsin, Trypsin+LysC, LysN, ArgC proteases suitable for different mass spec machines.

Try
ESI
ChTry
ESI
ArgC
ESI
LysN
ESI
TryLysC
ESI

Try
MALDI
ChTry
MALDI
ArgC
MALDI
LysN
MALDI
TryLysC
MALDI

Try
LTQ
ChTry
LTQ
ArgC
LTQ
LysN
LTQ
TryLysC
LTQ

Try
MSlow
ChTry
MSlow
ArgC
MSlow
LysN
MSlow
TryLysC
MSlow

Try
MShigh
ChTry
MShigh
ArgC
MShigh
LysN
MShigh
TryLysC
MShigh

General Statistics

Number of major isoforms

Number of additional isoforms

Number of all proteins

Number of amino acids

Min. Seq. Length

Max. Seq. Length

Avg. Seq. Length

Avg. Mol. Weight

2260

0

2260

802988

28

15867

355.3

38.44

Amino acid frequency

Ala

Cys

Asp

Glu

Phe

Gly

His

Ile

Lys

Leu

12.34 ± 0.099

1.538 ± 0.023

6.186 ± 0.046

6.506 ± 0.089

3.854 ± 0.047

8.464 ± 0.103

1.736 ± 0.024

4.751 ± 0.044

3.532 ± 0.045

9.242 ± 0.097

Met

Asn

Gln

Pro

Arg

Ser

Thr

Val

Trp

Tyr

2.545 ± 0.036

2.828 ± 0.082

4.38 ± 0.039

2.75 ± 0.031

6.071 ± 0.103

5.931 ± 0.078

5.247 ± 0.1

8.209 ± 0.055

1.071 ± 0.023

2.82 ± 0.036
Note: For amino acid frequency statistics the error has been estimated with the bootstraping (x100) at the protein level

Most of the basic statistics you can see at this page can be downloaded from this CSV file

For dipeptide frequency statistics click here
See this proteome in: uniprot_link
Proteome-pI is available under Creative Commons Attribution-NoDerivs license, for more details see here

Reference: Kozlowski LP. Proteome-pI 2.0: Proteome Isoelectric Point Database Update. Nucleic Acids Res. 2021, doi: 10.1093/nar/gkab944 Contact: Lukasz P. Kozlowski