Ichthyobacterium seriolicida
Average proteome isoelectric point is 6.91
Get precalculated fractions of proteins
Acidic
pI < 6.8
6.8-7.4
pI > 7.4
Basic
All
Note: above files contain also dissociation constants (pKa)
Virtual 2D-PAGE plot for 1467 proteins (isoelectric point calculated using IPC2_protein)
Get csv file with sequences according to given criteria:
* You can choose from 21 different methods for calculating isoelectric point
Summary statistics related to proteome-wise predictions
Protein with the lowest isoelectric point:
>tr|A0A1J1E8N2|A0A1J1E8N2_9FLAO Signal peptidase I OS=Ichthyobacterium seriolicida OX=242600 GN=JBKA6_0278 PE=3 SV=1
MM1 pKa = 6.82 NQVPKK6 pKa = 10.59 FLLADD11 pKa = 3.46 NSNYY15 pKa = 9.34 PDD17 pKa = 3.84 SIYY20 pKa = 10.89 VVHH23 pKa = 6.32 TEE25 pKa = 4.07 YY26 pKa = 10.46 PRR28 pKa = 11.84 FIYY31 pKa = 10.26 DD32 pKa = 3.5 ISNDD36 pKa = 4.0 EE37 pKa = 5.28 IILDD41 pKa = 4.24 DD42 pKa = 6.22 DD43 pKa = 5.72 DD44 pKa = 6.5 IEE46 pKa = 6.56 DD47 pKa = 3.82 EE48 pKa = 4.31 DD49 pKa = 5.08 KK50 pKa = 11.64 EE51 pKa = 4.68 NITQEE56 pKa = 3.92 LSSLIEE62 pKa = 3.96 EE63 pKa = 4.32 AQAFYY68 pKa = 10.82 DD69 pKa = 4.21 SEE71 pKa = 3.85 MSEE74 pKa = 4.09 YY75 pKa = 11.25 DD76 pKa = 3.19 KK77 pKa = 11.76
Molecular weight: 9.09 kDa
Isoelectric point according different methods:
IPC2.protein.svr19 3.776
IPC2_protein 3.605
IPC_protein 3.554
Toseland 3.35
ProMoST 3.745
Dawson 3.554
Bjellqvist 3.719
Wikipedia 3.503
Rodwell 3.401
Grimsley 3.274
Solomon 3.528
Lehninger 3.49
Nozaki 3.694
DTASelect 3.884
Thurlkill 3.427
EMBOSS 3.516
Sillero 3.681
Patrickios 0.769
IPC_peptide 3.528
IPC2_peptide 3.656
IPC2.peptide.svr19 3.676
Protein with the highest isoelectric point:
>tr|A0A1J1E9C5|A0A1J1E9C5_9FLAO Transposase OS=Ichthyobacterium seriolicida OX=242600 GN=JBKA6_0494 PE=4 SV=1
MM1 pKa = 7.24 GHH3 pKa = 7.48 LGLTPQSIYY12 pKa = 11.25 KK13 pKa = 10.27 LGTYY17 pKa = 9.6 KK18 pKa = 11.17 GEE20 pKa = 4.3 GKK22 pKa = 10.44 RR23 pKa = 11.84 IRR25 pKa = 11.84 GSQTAHH31 pKa = 6.07 RR32 pKa = 11.84 RR33 pKa = 11.84 CKK35 pKa = 9.55 RR36 pKa = 11.84 TLEE39 pKa = 3.68 SWMFCNSFRR48 pKa = 11.84 KK49 pKa = 10.12 NSIGTGKK56 pKa = 10.38 KK57 pKa = 8.6 EE58 pKa = 3.71
Molecular weight: 6.63 kDa
Isoelectric point according different methods:
IPC2.protein.svr19 9.31
IPC2_protein 9.78
IPC_protein 10.218
Toseland 10.789
ProMoST 10.35
Dawson 10.862
Bjellqvist 10.511
Wikipedia 11.008
Rodwell 11.316
Grimsley 10.891
Solomon 10.935
Lehninger 10.921
Nozaki 10.774
DTASelect 10.496
Thurlkill 10.76
EMBOSS 11.169
Sillero 10.789
Patrickios 11.082
IPC_peptide 10.95
IPC2_peptide 9.443
IPC2.peptide.svr19 8.572
Peptides (in silico digests for buttom-up proteomics)
Below you can find
in silico digests of the whole proteome with Trypsin, Chymotrypsin, Trypsin+LysC, LysN, ArgC proteases suitable for different mass spec machines.
Try ESI
ChTry ESI
ArgC ESI
LysN ESI
TryLysC ESI
Try MALDI
ChTry MALDI
ArgC MALDI
LysN MALDI
TryLysC MALDI
Try LTQ
ChTry LTQ
ArgC LTQ
LysN LTQ
TryLysC LTQ
Try MSlow
ChTry MSlow
ArgC MSlow
LysN MSlow
TryLysC MSlow
Try MShigh
ChTry MShigh
ArgC MShigh
LysN MShigh
TryLysC MShigh
General Statistics
Number of major isoforms
Number of additional isoforms
Number of all proteins
Number of amino acids
Min. Seq. Length
Max. Seq. Length
Avg. Seq. Length
Avg. Mol. Weight
1467
0
1467
549746
37
2339
374.7
41.94
Amino acid frequency
Ala
Cys
Asp
Glu
Phe
Gly
His
Ile
Lys
Leu
5.446 ± 0.132
0.782 ± 0.017
6.075 ± 0.04
6.268 ± 0.051
4.599 ± 0.059
6.39 ± 0.111
1.524 ± 0.024
8.896 ± 0.075
8.988 ± 0.056
8.139 ± 0.063
Met
Asn
Gln
Pro
Arg
Ser
Thr
Val
Trp
Tyr
1.951 ± 0.039
6.437 ± 0.069
3.118 ± 0.046
2.55 ± 0.038
3.32 ± 0.046
8.457 ± 0.066
6.443 ± 0.111
6.145 ± 0.053
0.696 ± 0.019
3.772 ± 0.048
Note: For amino acid frequency statistics the error has been estimated with the bootstraping (x100) at the protein level
Most of the basic statistics you can see at this page can be downloaded from this CSV file
For dipeptide frequency statistics click here