Alternaria arborescens victorivirus 1
Average proteome isoelectric point is 6.85
Get precalculated fractions of proteins
Acidic
pI < 6.8
6.8-7.4
pI > 7.4
Basic
All
Note: above files contain also dissociation constants (pKa)
Virtual 2D-PAGE plot for 2 proteins (isoelectric point calculated using IPC2_protein)
Get csv file with sequences according to given criteria:
* You can choose from 21 different methods for calculating isoelectric point
Summary statistics related to proteome-wise predictions
Protein with the lowest isoelectric point:
>tr|A0A146HTF4|A0A146HTF4_9VIRU RNA-directed RNA polymerase OS=Alternaria arborescens victorivirus 1 OX=1728964 GN=RdRp PE=3 SV=1
MM1 pKa = 7.85 ASTTRR6 pKa = 11.84 FLASGASPLTGTVAGISAGTIQADD30 pKa = 3.51 NQYY33 pKa = 10.33 RR34 pKa = 11.84 RR35 pKa = 11.84 YY36 pKa = 10.2 RR37 pKa = 11.84 SGLTIGVHH45 pKa = 4.53 EE46 pKa = 5.22 HH47 pKa = 6.81 GSVTQYY53 pKa = 10.36 RR54 pKa = 11.84 RR55 pKa = 11.84 SIFYY59 pKa = 10.31 EE60 pKa = 3.51 VGRR63 pKa = 11.84 RR64 pKa = 11.84 YY65 pKa = 10.51 GRR67 pKa = 11.84 LNDD70 pKa = 3.66 ALGAHH75 pKa = 6.79 GDD77 pKa = 3.65 EE78 pKa = 5.15 VIPVDD83 pKa = 3.44 ASVRR87 pKa = 11.84 INAAEE92 pKa = 3.86 AANFEE97 pKa = 4.18 GFARR101 pKa = 11.84 RR102 pKa = 11.84 FSNFSPQWVMMDD114 pKa = 3.85 LAGIAEE120 pKa = 4.2 RR121 pKa = 11.84 LAKK124 pKa = 10.17 GVAAQSIFGGVTITNLRR141 pKa = 11.84 AHH143 pKa = 5.6 QPVRR147 pKa = 11.84 VVALGTLDD155 pKa = 4.0 SPQTASNSSVFIPRR169 pKa = 11.84 TVDD172 pKa = 3.11 TVGNDD177 pKa = 2.9 HH178 pKa = 6.28 VFAVLVAAANGEE190 pKa = 4.33 GAAVTTDD197 pKa = 3.57 VLRR200 pKa = 11.84 LDD202 pKa = 4.49 ANTNEE207 pKa = 4.78 PIVPAVSGHH216 pKa = 6.58 ALGTACVEE224 pKa = 3.91 ALRR227 pKa = 11.84 IIGANMEE234 pKa = 4.09 ASGAGDD240 pKa = 3.18 IFSYY244 pKa = 10.88 AVTRR248 pKa = 11.84 GVHH251 pKa = 5.83 ALVSVVGHH259 pKa = 5.59 TDD261 pKa = 2.52 EE262 pKa = 4.73 GGYY265 pKa = 8.61 MRR267 pKa = 11.84 EE268 pKa = 3.74 LLRR271 pKa = 11.84 YY272 pKa = 8.72 GRR274 pKa = 11.84 FRR276 pKa = 11.84 VPYY279 pKa = 10.08 GGINQSLRR287 pKa = 11.84 DD288 pKa = 4.15 YY289 pKa = 10.62 PSLPAAGSLSASTMSAWVDD308 pKa = 4.23 AIALKK313 pKa = 9.23 TAAIVAHH320 pKa = 6.96 CDD322 pKa = 3.37 PTVTASGGVYY332 pKa = 8.28 PTVFTAAAGDD342 pKa = 3.85 VSPPGTDD349 pKa = 3.32 EE350 pKa = 5.34 GDD352 pKa = 3.69 TPTDD356 pKa = 3.41 ADD358 pKa = 3.24 GRR360 pKa = 11.84 AIGRR364 pKa = 11.84 QVSGDD369 pKa = 3.58 LGRR372 pKa = 11.84 FAPLYY377 pKa = 10.06 LHH379 pKa = 6.75 GLTRR383 pKa = 11.84 IFGLNTNSQVAEE395 pKa = 3.83 AHH397 pKa = 5.91 FCTVAGAYY405 pKa = 8.54 LANNVDD411 pKa = 2.7 RR412 pKa = 11.84 HH413 pKa = 5.67 LRR415 pKa = 11.84 HH416 pKa = 5.82 KK417 pKa = 9.35 TVAPYY422 pKa = 9.8 FWIEE426 pKa = 3.79 PTSLIDD432 pKa = 3.67 VGFLGSPAEE441 pKa = 4.03 AAGFGALATPGEE453 pKa = 4.38 TAHH456 pKa = 6.73 IPTFEE461 pKa = 4.03 RR462 pKa = 11.84 HH463 pKa = 5.73 RR464 pKa = 11.84 EE465 pKa = 3.66 LDD467 pKa = 2.9 RR468 pKa = 11.84 GRR470 pKa = 11.84 NANFSTLAFKK480 pKa = 10.28 MRR482 pKa = 11.84 TARR485 pKa = 11.84 TSGLIAAHH493 pKa = 6.53 AASPAPLAHH502 pKa = 6.16 MKK504 pKa = 10.27 LYY506 pKa = 10.79 QFDD509 pKa = 4.42 DD510 pKa = 4.29 DD511 pKa = 4.72 SVILAGDD518 pKa = 3.57 QVPTRR523 pKa = 11.84 GDD525 pKa = 3.17 VRR527 pKa = 11.84 VKK529 pKa = 10.53 HH530 pKa = 5.97 SAADD534 pKa = 3.76 PLSSYY539 pKa = 10.76 LWVRR543 pKa = 11.84 GQSAIPAPAEE553 pKa = 4.05 FINIQGSYY561 pKa = 8.37 AAKK564 pKa = 10.24 YY565 pKa = 9.71 MNVDD569 pKa = 2.72 WSDD572 pKa = 4.0 DD573 pKa = 3.48 FDD575 pKa = 4.0 GTVTDD580 pKa = 5.85 LPEE583 pKa = 4.27 AWEE586 pKa = 4.73 LEE588 pKa = 4.18 HH589 pKa = 6.69 DD590 pKa = 4.12 TQWRR594 pKa = 11.84 VTVPTGLSVTGTSNYY609 pKa = 10.07 ADD611 pKa = 3.54 RR612 pKa = 11.84 EE613 pKa = 4.16 ARR615 pKa = 11.84 RR616 pKa = 11.84 ARR618 pKa = 11.84 SRR620 pKa = 11.84 AAIALAQAALRR631 pKa = 11.84 ARR633 pKa = 11.84 AYY635 pKa = 10.5 GEE637 pKa = 4.19 ANSPVIDD644 pKa = 4.03 VSNVPPTWDD653 pKa = 3.31 EE654 pKa = 3.38 EE655 pKa = 4.31 RR656 pKa = 11.84 APVTTWHH663 pKa = 6.28 GVNHH667 pKa = 6.7 HH668 pKa = 6.99 SDD670 pKa = 3.31 PGITAGRR677 pKa = 11.84 GEE679 pKa = 4.61 GAPPDD684 pKa = 3.61 TTNPVRR690 pKa = 11.84 GPAMVPIPHH699 pKa = 6.41 HH700 pKa = 5.66 QPLRR704 pKa = 11.84 GAPYY708 pKa = 8.13 TRR710 pKa = 11.84 GGGVVGGGGPPAPPAGGAGGPPPPHH735 pKa = 6.2 NQGPPPPPEE744 pKa = 5.92 GGMDD748 pKa = 3.77 LGDD751 pKa = 3.84 GAAAAAALAAGGEE764 pKa = 4.12 QGAAGAQPAPQVV776 pKa = 3.31
Molecular weight: 80.93 kDa
Isoelectric point according different methods:
IPC2.protein.svr19 5.769
IPC2_protein 5.601
IPC_protein 5.677
Toseland 6.224
ProMoST 6.059
Dawson 5.97
Bjellqvist 5.97
Wikipedia 5.982
Rodwell 5.957
Grimsley 6.44
Solomon 5.97
Lehninger 5.957
Nozaki 6.237
DTASelect 6.44
Thurlkill 6.453
EMBOSS 6.415
Sillero 6.338
Patrickios 4.177
IPC_peptide 5.982
IPC2_peptide 6.364
IPC2.peptide.svr19 6.219
Protein with the highest isoelectric point:
>tr|A0A146HTF4|A0A146HTF4_9VIRU RNA-directed RNA polymerase OS=Alternaria arborescens victorivirus 1 OX=1728964 GN=RdRp PE=3 SV=1
MM1 pKa = 7.68 IDD3 pKa = 3.55 SPVTDD8 pKa = 3.79 RR9 pKa = 11.84 ANVAGAVGEE18 pKa = 4.1 YY19 pKa = 10.68 LKK21 pKa = 11.18 VLTDD25 pKa = 3.72 DD26 pKa = 4.23 RR27 pKa = 11.84 DD28 pKa = 3.62 VSFLLSSPFSEE39 pKa = 4.25 QISYY43 pKa = 9.99 IYY45 pKa = 9.9 KK46 pKa = 9.79 PSWGGRR52 pKa = 11.84 KK53 pKa = 7.22 PTPLRR58 pKa = 11.84 RR59 pKa = 11.84 AALSFLCATVPVQVPIPEE77 pKa = 4.33 SYY79 pKa = 10.46 LWQLVDD85 pKa = 3.49 ATCPVPEE92 pKa = 4.33 LSIRR96 pKa = 11.84 MDD98 pKa = 4.27 YY99 pKa = 9.22 KK100 pKa = 10.66 WKK102 pKa = 7.75 WAKK105 pKa = 10.25 NAGEE109 pKa = 4.25 TVARR113 pKa = 11.84 YY114 pKa = 8.25 PLKK117 pKa = 10.73 KK118 pKa = 10.55 NPAASNKK125 pKa = 9.43 VNLFLYY131 pKa = 7.54 EE132 pKa = 3.89 VCRR135 pKa = 11.84 DD136 pKa = 3.56 LAKK139 pKa = 10.54 KK140 pKa = 8.88 DD141 pKa = 3.58 TNALRR146 pKa = 11.84 TGLTYY151 pKa = 10.74 LEE153 pKa = 4.41 WLRR156 pKa = 11.84 DD157 pKa = 3.66 RR158 pKa = 11.84 QVVYY162 pKa = 10.9 NDD164 pKa = 2.88 QATAVLLYY172 pKa = 9.63 GYY174 pKa = 9.59 CLSRR178 pKa = 11.84 YY179 pKa = 8.78 HH180 pKa = 5.27 PHH182 pKa = 5.74 GWRR185 pKa = 11.84 HH186 pKa = 5.68 ALASLTPSSYY196 pKa = 10.94 TKK198 pKa = 10.54 GLSVFGKK205 pKa = 10.39 AVGINGTPVGAMLVEE220 pKa = 4.11 ATVLLGRR227 pKa = 11.84 DD228 pKa = 3.63 VAPTDD233 pKa = 3.64 LVEE236 pKa = 4.15 EE237 pKa = 3.83 ARR239 pKa = 11.84 MRR241 pKa = 11.84 VTEE244 pKa = 4.16 SGVSKK249 pKa = 10.64 VVAPYY254 pKa = 10.34 PEE256 pKa = 4.47 DD257 pKa = 3.62 VMRR260 pKa = 11.84 RR261 pKa = 11.84 AVRR264 pKa = 11.84 KK265 pKa = 9.68 NLLSEE270 pKa = 4.65 LKK272 pKa = 10.57 RR273 pKa = 11.84 SGDD276 pKa = 3.62 SYY278 pKa = 11.63 KK279 pKa = 10.99 LEE281 pKa = 4.35 FPTLEE286 pKa = 4.21 DD287 pKa = 2.58 HH288 pKa = 7.13 WATRR292 pKa = 11.84 WRR294 pKa = 11.84 WAVNGAHH301 pKa = 6.62 SGLIYY306 pKa = 10.31 KK307 pKa = 10.05 RR308 pKa = 11.84 NPSLRR313 pKa = 11.84 TTPPGFDD320 pKa = 3.55 RR321 pKa = 11.84 IHH323 pKa = 6.07 RR324 pKa = 11.84 RR325 pKa = 11.84 AWLEE329 pKa = 3.95 TVDD332 pKa = 4.73 KK333 pKa = 10.77 DD334 pKa = 5.41 PRR336 pKa = 11.84 PDD338 pKa = 3.42 WDD340 pKa = 3.24 GKK342 pKa = 8.77 TYY344 pKa = 11.02 VSASPKK350 pKa = 10.56 LEE352 pKa = 4.28 LGKK355 pKa = 8.72 TRR357 pKa = 11.84 AIFACDD363 pKa = 3.75 TINYY367 pKa = 9.02 LAFEE371 pKa = 4.67 HH372 pKa = 6.69 LMSTVEE378 pKa = 4.32 SNWRR382 pKa = 11.84 GEE384 pKa = 4.16 RR385 pKa = 11.84 IILNPGKK392 pKa = 10.43 GGHH395 pKa = 6.83 LGMANRR401 pKa = 11.84 VRR403 pKa = 11.84 AARR406 pKa = 11.84 NRR408 pKa = 11.84 SGVSLMLDD416 pKa = 3.09 YY417 pKa = 11.57 DD418 pKa = 4.5 DD419 pKa = 6.0 FNSHH423 pKa = 6.22 HH424 pKa = 6.19 TTQSMRR430 pKa = 11.84 IVLEE434 pKa = 4.06 EE435 pKa = 3.95 LCSITEE441 pKa = 4.1 YY442 pKa = 9.3 PTDD445 pKa = 3.88 LAEE448 pKa = 5.09 KK449 pKa = 9.94 LIKK452 pKa = 10.82 SLDD455 pKa = 3.63 DD456 pKa = 3.13 EE457 pKa = 5.13 HH458 pKa = 8.81 IYY460 pKa = 10.87 VAGKK464 pKa = 10.45 YY465 pKa = 7.88 MGRR468 pKa = 11.84 VLGTLMSGHH477 pKa = 7.19 RR478 pKa = 11.84 LTTFFNSVLNKK489 pKa = 10.32 AYY491 pKa = 10.83 LDD493 pKa = 3.73 IEE495 pKa = 4.58 LGDD498 pKa = 5.42 DD499 pKa = 3.89 IMDD502 pKa = 3.91 NCVSLHH508 pKa = 6.3 VGDD511 pKa = 5.06 DD512 pKa = 3.49 VYY514 pKa = 11.6 LGVRR518 pKa = 11.84 SYY520 pKa = 11.61 KK521 pKa = 10.33 EE522 pKa = 3.54 ADD524 pKa = 3.47 VVLSRR529 pKa = 11.84 IRR531 pKa = 11.84 TSRR534 pKa = 11.84 LRR536 pKa = 11.84 MNPAKK541 pKa = 10.39 QSVGHH546 pKa = 5.33 VTTEE550 pKa = 3.83 FLRR553 pKa = 11.84 IASEE557 pKa = 3.97 SRR559 pKa = 11.84 YY560 pKa = 10.33 SYY562 pKa = 11.07 GYY564 pKa = 9.69 LARR567 pKa = 11.84 SVASITSGNWVSEE580 pKa = 4.2 AKK582 pKa = 10.4 LSPLEE587 pKa = 4.5 ALNSMLNSARR597 pKa = 11.84 SLANRR602 pKa = 11.84 SGLADD607 pKa = 3.85 APLLLVTSVSRR618 pKa = 11.84 MAPLASKK625 pKa = 10.81 DD626 pKa = 3.58 DD627 pKa = 3.93 ALVRR631 pKa = 11.84 EE632 pKa = 4.39 LLLGKK637 pKa = 9.77 IALGNGPQFQSSGTYY652 pKa = 9.44 RR653 pKa = 11.84 YY654 pKa = 10.7 VNLRR658 pKa = 11.84 PEE660 pKa = 4.65 FKK662 pKa = 10.64 HH663 pKa = 7.77 IDD665 pKa = 3.36 DD666 pKa = 4.19 NGYY669 pKa = 10.91 GKK671 pKa = 10.63 LPLMATHH678 pKa = 7.47 TYY680 pKa = 8.04 LTSAATEE687 pKa = 4.28 LEE689 pKa = 4.3 TEE691 pKa = 4.18 VLIRR695 pKa = 11.84 AGVSVEE701 pKa = 3.56 EE702 pKa = 4.18 DD703 pKa = 3.11 MARR706 pKa = 11.84 ASYY709 pKa = 10.72 AKK711 pKa = 10.01 SAPQALFTEE720 pKa = 4.5 EE721 pKa = 4.11 RR722 pKa = 11.84 LVVQHH727 pKa = 7.02 RR728 pKa = 11.84 GQKK731 pKa = 9.76 NVVGVEE737 pKa = 3.69 WAEE740 pKa = 3.9 MVLRR744 pKa = 11.84 QPRR747 pKa = 11.84 VIGLLTPYY755 pKa = 10.24 PLLLLARR762 pKa = 11.84 ARR764 pKa = 11.84 LPEE767 pKa = 3.98 HH768 pKa = 6.44 LVRR771 pKa = 11.84 YY772 pKa = 9.14 ALHH775 pKa = 5.96 QAGGDD780 pKa = 3.67 YY781 pKa = 7.62 NTPYY785 pKa = 10.76 LEE787 pKa = 3.84 YY788 pKa = 10.55 DD789 pKa = 2.65 AWGEE793 pKa = 4.03 YY794 pKa = 10.73 AHH796 pKa = 6.88 GCVIDD801 pKa = 3.86 TVMSYY806 pKa = 10.55 TDD808 pKa = 3.65 ASALGSRR815 pKa = 11.84 TAAGVLTSVNRR826 pKa = 11.84 MYY828 pKa = 11.24 VV829 pKa = 2.87
Molecular weight: 92.52 kDa
Isoelectric point according different methods:
IPC2.protein.svr19 7.925
IPC2_protein 8.126
IPC_protein 8.009
Toseland 8.141
ProMoST 8.478
Dawson 8.741
Bjellqvist 8.829
Wikipedia 8.843
Rodwell 8.785
Grimsley 8.361
Solomon 8.814
Lehninger 8.785
Nozaki 8.814
DTASelect 8.682
Thurlkill 8.712
EMBOSS 8.887
Sillero 8.931
Patrickios 4.304
IPC_peptide 8.799
IPC2_peptide 7.658
IPC2.peptide.svr19 7.751
Peptides (in silico digests for buttom-up proteomics)
Below you can find
in silico digests of the whole proteome with Trypsin, Chymotrypsin, Trypsin+LysC, LysN, ArgC proteases suitable for different mass spec machines.
Try ESI
ChTry ESI
ArgC ESI
LysN ESI
TryLysC ESI
Try MALDI
ChTry MALDI
ArgC MALDI
LysN MALDI
TryLysC MALDI
Try LTQ
ChTry LTQ
ArgC LTQ
LysN LTQ
TryLysC LTQ
Try MSlow
ChTry MSlow
ArgC MSlow
LysN MSlow
TryLysC MSlow
Try MShigh
ChTry MShigh
ArgC MShigh
LysN MShigh
TryLysC MShigh
General Statistics
Number of major isoforms
Number of additional isoforms
Number of all proteins
Number of amino acids
Min. Seq. Length
Max. Seq. Length
Avg. Seq. Length
Avg. Mol. Weight
2
0
2
1605
776
829
802.5
86.72
Amino acid frequency
Ala
Cys
Asp
Glu
Phe
Gly
His
Ile
Lys
Leu
11.838 ± 2.318
0.685 ± 0.223
5.483 ± 0.044
4.673 ± 0.601
2.492 ± 0.448
8.785 ± 1.809
2.804 ± 0.312
3.551 ± 0.331
2.679 ± 1.325
9.034 ± 2.123
Met
Asn
Gln
Pro
Arg
Ser
Thr
Val
Trp
Tyr
1.931 ± 0.383
3.614 ± 0.1
6.106 ± 1.02
2.181 ± 0.392
7.165 ± 0.346
6.978 ± 0.687
6.48 ± 0.357
8.162 ± 0.128
1.495 ± 0.25
3.863 ± 0.862
Note: For amino acid frequency statistics the error has been estimated with the bootstraping (x100) at the protein level
Most of the basic statistics you can see at this page can be downloaded from this CSV file
For dipeptide frequency statistics click here