Blastococcus sp. CT_GayMR19

Taxonomy: cellular organisms; Bacteria; Terrabacteria group; Actinobacteria; Actinomycetia; Geodermatophilales; Geodermatophilaceae; Blastococcus; unclassified Blastococcus

Average proteome isoelectric point is 6.08

Get precalculated fractions of proteins

Acidic
pI < 6.8
6.8-7.4
pI > 7.4
Basic
    
All



Virtual 2D-PAGE plot for 4236 proteins (isoelectric point calculated using IPC2_protein)

Get csv file with sequences according to given criteria:
        -     Method 

     -  kDa    
                                                                                      

* You can choose from 21 different methods for calculating isoelectric point

Summary statistics related to proteome-wise predictions


    
Protein with the lowest isoelectric point:
>tr|A0A4Y9PFB3|A0A4Y9PFB3_9ACTN S_2TMBeta domain-containing protein OS=Blastococcus sp. CT_GayMR19 OX=2559608 GN=E4P39_03420 PE=4 SV=1
MM1 pKa = 6.54TACGGSDD8 pKa = 3.4EE9 pKa = 4.61EE10 pKa = 5.01GSGEE14 pKa = 4.56GEE16 pKa = 3.97AAEE19 pKa = 4.06QAGGTFSMYY28 pKa = 10.18IGQPEE33 pKa = 4.15NPLVPGNTNEE43 pKa = 4.28TEE45 pKa = 4.22GGQVLDD51 pKa = 3.86SLFTGLVQYY60 pKa = 10.6DD61 pKa = 3.94AEE63 pKa = 4.33TNEE66 pKa = 4.16AVFSGVAASIEE77 pKa = 4.18SDD79 pKa = 4.73DD80 pKa = 3.86KK81 pKa = 10.86QTWTVTLNEE90 pKa = 3.94GWTFHH95 pKa = 7.57DD96 pKa = 4.41GTDD99 pKa = 3.23VTAQSFVDD107 pKa = 3.45AWNYY111 pKa = 7.22TAYY114 pKa = 10.76SPNAQGNSYY123 pKa = 10.41FFANVAGYY131 pKa = 11.23GDD133 pKa = 4.38LQAEE137 pKa = 4.27TDD139 pKa = 3.99DD140 pKa = 4.23NDD142 pKa = 3.83VVIAPPVAEE151 pKa = 4.12QMSGLRR157 pKa = 11.84VVDD160 pKa = 4.2DD161 pKa = 3.6LTFEE165 pKa = 4.43VEE167 pKa = 4.16LTSPYY172 pKa = 10.19AQWPTTVGYY181 pKa = 7.89TAFFPLPEE189 pKa = 4.67AFFADD194 pKa = 3.67PEE196 pKa = 4.76GFGEE200 pKa = 4.15QPIGNGPFQADD211 pKa = 3.82EE212 pKa = 4.49PFVPDD217 pKa = 4.22EE218 pKa = 5.07GITLTKK224 pKa = 10.11YY225 pKa = 10.42EE226 pKa = 4.74EE227 pKa = 4.33YY228 pKa = 11.0AGDD231 pKa = 3.75EE232 pKa = 4.24PANADD237 pKa = 3.4SVKK240 pKa = 9.7YY241 pKa = 9.92VVYY244 pKa = 10.73AEE246 pKa = 4.27QEE248 pKa = 3.98TAYY251 pKa = 9.84TDD253 pKa = 3.73LQGGQVDD260 pKa = 4.52IMDD263 pKa = 5.23TLPPDD268 pKa = 5.39AIASAPDD275 pKa = 3.47EE276 pKa = 4.6FGDD279 pKa = 4.63RR280 pKa = 11.84YY281 pKa = 10.53IEE283 pKa = 4.07TAQGDD288 pKa = 3.84ITSLGFPTYY297 pKa = 9.52DD298 pKa = 4.34QRR300 pKa = 11.84FADD303 pKa = 3.84PNVRR307 pKa = 11.84KK308 pKa = 9.59AFSMAIDD315 pKa = 3.7RR316 pKa = 11.84QAISDD321 pKa = 4.26AIFNGTRR328 pKa = 11.84TPATSFISPVVKK340 pKa = 10.04GHH342 pKa = 7.19RR343 pKa = 11.84DD344 pKa = 3.49DD345 pKa = 4.4ACEE348 pKa = 3.9VCEE351 pKa = 5.24LNVEE355 pKa = 4.36EE356 pKa = 5.29ANSLLDD362 pKa = 3.24EE363 pKa = 5.04AGFDD367 pKa = 3.37RR368 pKa = 11.84SQPVDD373 pKa = 2.84LWFNAGAGHH382 pKa = 6.42EE383 pKa = 4.27GWMEE387 pKa = 3.67AVGNQIRR394 pKa = 11.84EE395 pKa = 4.07NLDD398 pKa = 2.77VEE400 pKa = 4.44FQLRR404 pKa = 11.84GDD406 pKa = 4.18LQQSEE411 pKa = 4.59FLPLKK416 pKa = 9.59DD417 pKa = 3.82AKK419 pKa = 11.18GMTGPFRR426 pKa = 11.84DD427 pKa = 4.21AWVMDD432 pKa = 3.57YY433 pKa = 10.78PVAEE437 pKa = 4.34NFLGPLYY444 pKa = 10.73SSAALPPAGSNYY456 pKa = 9.03TFYY459 pKa = 11.44SNPEE463 pKa = 3.31FDD465 pKa = 3.93ALLVEE470 pKa = 5.29GNSASNDD477 pKa = 2.98EE478 pKa = 4.46DD479 pKa = 6.1AIAAYY484 pKa = 9.64QAAEE488 pKa = 4.93DD489 pKa = 3.98ILLEE493 pKa = 4.63DD494 pKa = 5.06FPSAPLFYY502 pKa = 10.61RR503 pKa = 11.84LNQGAHH509 pKa = 5.63SEE511 pKa = 4.1NVEE514 pKa = 4.0NVIIDD519 pKa = 3.32AFGRR523 pKa = 11.84IDD525 pKa = 3.23AAAVRR530 pKa = 11.84VVGG533 pKa = 4.26

Molecular weight: 57.66 kDa
Isoelectric point according different methods:





Protein with the highest isoelectric point:
>tr|A0A4Y9NRH4|A0A4Y9NRH4_9ACTN ATP-binding protein OS=Blastococcus sp. CT_GayMR19 OX=2559608 GN=E4P39_19845 PE=3 SV=1
MM1 pKa = 7.1PHH3 pKa = 6.5SGASATGRR11 pKa = 11.84WRR13 pKa = 11.84GPPQPLRR20 pKa = 11.84RR21 pKa = 11.84WPPPRR26 pKa = 11.84PPQPRR31 pKa = 11.84WPRR34 pKa = 11.84WPPRR38 pKa = 11.84LPAPPRR44 pKa = 11.84RR45 pKa = 11.84CRR47 pKa = 11.84RR48 pKa = 11.84TGRR51 pKa = 11.84RR52 pKa = 11.84RR53 pKa = 11.84VPLRR57 pKa = 11.84PHH59 pKa = 6.62SAPGPRR65 pKa = 11.84RR66 pKa = 11.84RR67 pKa = 11.84RR68 pKa = 11.84RR69 pKa = 11.84RR70 pKa = 11.84PLRR73 pKa = 11.84PRR75 pKa = 11.84RR76 pKa = 11.84PWAPRR81 pKa = 11.84RR82 pKa = 11.84GRR84 pKa = 11.84RR85 pKa = 11.84PPPSWTAGSPPHH97 pKa = 6.8PAPAGLNTAPRR108 pKa = 11.84GRR110 pKa = 11.84AGRR113 pKa = 11.84PGAGG117 pKa = 3.17

Molecular weight: 13.33 kDa
Isoelectric point according different methods:





Peptides (in silico digests for buttom-up proteomics)

Below you can find in silico digests of the whole proteome with Trypsin, Chymotrypsin, Trypsin+LysC, LysN, ArgC proteases suitable for different mass spec machines.

Try
ESI
ChTry
ESI
ArgC
ESI
LysN
ESI
TryLysC
ESI

Try
MALDI
ChTry
MALDI
ArgC
MALDI
LysN
MALDI
TryLysC
MALDI

Try
LTQ
ChTry
LTQ
ArgC
LTQ
LysN
LTQ
TryLysC
LTQ

Try
MSlow
ChTry
MSlow
ArgC
MSlow
LysN
MSlow
TryLysC
MSlow

Try
MShigh
ChTry
MShigh
ArgC
MShigh
LysN
MShigh
TryLysC
MShigh

General Statistics

Number of major isoforms

Number of additional isoforms

Number of all proteins

Number of amino acids

Min. Seq. Length

Max. Seq. Length

Avg. Seq. Length

Avg. Mol. Weight

4236

0

4236

1347984

32

1957

318.2

33.79

Amino acid frequency

Ala

Cys

Asp

Glu

Phe

Gly

His

Ile

Lys

Leu

14.048 ± 0.061

0.699 ± 0.008

6.196 ± 0.03

5.651 ± 0.032

2.663 ± 0.022

9.673 ± 0.037

1.99 ± 0.018

3.09 ± 0.028

1.514 ± 0.026

10.65 ± 0.049

Met

Asn

Gln

Pro

Arg

Ser

Thr

Val

Trp

Tyr

1.697 ± 0.016

1.552 ± 0.017

6.182 ± 0.032

2.651 ± 0.021

8.008 ± 0.042

5.054 ± 0.026

5.906 ± 0.028

9.564 ± 0.036

1.431 ± 0.017

1.781 ± 0.016
Note: For amino acid frequency statistics the error has been estimated with the bootstraping (x100) at the protein level

Most of the basic statistics you can see at this page can be downloaded from this CSV file

For dipeptide frequency statistics click here
See this proteome in: uniprot_link
Proteome-pI is available under Creative Commons Attribution-NoDerivs license, for more details see here

Reference: Kozlowski LP. Proteome-pI 2.0: Proteome Isoelectric Point Database Update. Nucleic Acids Res. 2021, doi: 10.1093/nar/gkab944 Contact: Lukasz P. Kozlowski