Corynebacterium diphtheriae (strain ATCC 700971 / NCTC 13129 / Biotype gravis)

Taxonomy: cellular organisms; Bacteria; Terrabacteria group; Actinobacteria; Actinomycetia; Corynebacteriales; Corynebacteriaceae; Corynebacterium; Corynebacterium diphtheriae

Average proteome isoelectric point is 6.2

Get precalculated fractions of proteins

Acidic
pI < 6.8
6.8-7.4
pI > 7.4
Basic
    
All



Virtual 2D-PAGE plot for 2265 proteins (isoelectric point calculated using IPC2_protein)

Get csv file with sequences according to given criteria:
        -     Method 

     -  kDa    
                                                                                      

* You can choose from 21 different methods for calculating isoelectric point

Summary statistics related to proteome-wise predictions


    
Protein with the lowest isoelectric point:
>tr|Q6NIG7|Q6NIG7_CORDI Uncharacterized protein OS=Corynebacterium diphtheriae (strain ATCC 700971 / NCTC 13129 / Biotype gravis) OX=257309 GN=DIP0808 PE=4 SV=1
MM1 pKa = 7.37AAVAGDD7 pKa = 3.67TVPFVPARR15 pKa = 11.84RR16 pKa = 11.84DD17 pKa = 3.31VTCADD22 pKa = 3.42EE23 pKa = 4.44VTAVGSFFRR32 pKa = 11.84YY33 pKa = 7.32TGHH36 pKa = 7.01VIDD39 pKa = 4.65GQGHH43 pKa = 4.5VRR45 pKa = 11.84LEE47 pKa = 4.21EE48 pKa = 3.99VVDD51 pKa = 4.25PVDD54 pKa = 3.14TDD56 pKa = 3.84EE57 pKa = 6.9DD58 pKa = 4.26VDD60 pKa = 5.73CCDD63 pKa = 3.23GWCDD67 pKa = 3.4EE68 pKa = 4.48TQCFLFEE75 pKa = 5.06EE76 pKa = 5.42DD77 pKa = 5.65DD78 pKa = 4.4DD79 pKa = 4.77DD80 pKa = 4.62

Molecular weight: 8.82 kDa
Isoelectric point according different methods:





Protein with the highest isoelectric point:
>tr|Q6NEA7|Q6NEA7_CORDI Nudix hydrolase domain-containing protein OS=Corynebacterium diphtheriae (strain ATCC 700971 / NCTC 13129 / Biotype gravis) OX=257309 GN=DIP2369 PE=3 SV=1
MM1 pKa = 7.69AKK3 pKa = 10.06GKK5 pKa = 8.69RR6 pKa = 11.84TFQPNNRR13 pKa = 11.84RR14 pKa = 11.84RR15 pKa = 11.84ARR17 pKa = 11.84KK18 pKa = 8.01HH19 pKa = 4.51GFRR22 pKa = 11.84IRR24 pKa = 11.84MRR26 pKa = 11.84TRR28 pKa = 11.84AGRR31 pKa = 11.84AIVAARR37 pKa = 11.84RR38 pKa = 11.84NKK40 pKa = 9.24GRR42 pKa = 11.84KK43 pKa = 9.07SLTAA47 pKa = 4.07

Molecular weight: 5.51 kDa
Isoelectric point according different methods:





Peptides (in silico digests for buttom-up proteomics)

Below you can find in silico digests of the whole proteome with Trypsin, Chymotrypsin, Trypsin+LysC, LysN, ArgC proteases suitable for different mass spec machines.

Try
ESI
ChTry
ESI
ArgC
ESI
LysN
ESI
TryLysC
ESI

Try
MALDI
ChTry
MALDI
ArgC
MALDI
LysN
MALDI
TryLysC
MALDI

Try
LTQ
ChTry
LTQ
ArgC
LTQ
LysN
LTQ
TryLysC
LTQ

Try
MSlow
ChTry
MSlow
ArgC
MSlow
LysN
MSlow
TryLysC
MSlow

Try
MShigh
ChTry
MShigh
ArgC
MShigh
LysN
MShigh
TryLysC
MShigh

General Statistics

Number of major isoforms

Number of additional isoforms

Number of all proteins

Number of amino acids

Min. Seq. Length

Max. Seq. Length

Avg. Seq. Length

Avg. Mol. Weight

2265

0

2265

725308

31

2977

320.2

34.83

Amino acid frequency

Ala

Cys

Asp

Glu

Phe

Gly

His

Ile

Lys

Leu

11.075 ± 0.071

0.848 ± 0.015

5.854 ± 0.045

5.885 ± 0.057

3.366 ± 0.037

7.903 ± 0.042

2.4 ± 0.027

5.622 ± 0.042

3.667 ± 0.059

9.388 ± 0.061

Met

Asn

Gln

Pro

Arg

Ser

Thr

Val

Trp

Tyr

2.318 ± 0.026

3.095 ± 0.035

4.872 ± 0.038

3.482 ± 0.028

5.943 ± 0.049

6.235 ± 0.039

6.113 ± 0.029

8.245 ± 0.052

1.409 ± 0.026

2.28 ± 0.028
Note: For amino acid frequency statistics the error has been estimated with the bootstraping (x100) at the protein level

Most of the basic statistics you can see at this page can be downloaded from this CSV file

For dipeptide frequency statistics click here
See this proteome in: uniprot_link
Proteome-pI is available under Creative Commons Attribution-NoDerivs license, for more details see here

Reference: Kozlowski LP. Proteome-pI 2.0: Proteome Isoelectric Point Database Update. Nucleic Acids Res. 2021, doi: 10.1093/nar/gkab944 Contact: Lukasz P. Kozlowski