Clostridium carboxidivorans P7
Average proteome isoelectric point is 6.72
Get precalculated fractions of proteins
Acidic
pI < 6.8
6.8-7.4
pI > 7.4
Basic
All
Note: above files contain also dissociation constants (pKa)
Virtual 2D-PAGE plot for 5458 proteins (isoelectric point calculated using IPC2_protein)
Get csv file with sequences according to given criteria:
* You can choose from 21 different methods for calculating isoelectric point
Summary statistics related to proteome-wise predictions
Protein with the lowest isoelectric point:
>tr|C6PQT8|C6PQT8_9CLOT Uncharacterized protein OS=Clostridium carboxidivorans P7 OX=536227 GN=CcarbDRAFT_1155 PE=4 SV=1
MM1 pKa = 7.69 SDD3 pKa = 3.8 IISKK7 pKa = 9.85 VAYY10 pKa = 10.7 LNGLVDD16 pKa = 4.05 GLEE19 pKa = 3.98 IDD21 pKa = 3.72 TTTKK25 pKa = 9.89 EE26 pKa = 4.2 GKK28 pKa = 9.48 VLNEE32 pKa = 4.3 IINVLKK38 pKa = 10.98 VIAEE42 pKa = 4.16 EE43 pKa = 3.79 VDD45 pKa = 4.04 EE46 pKa = 4.67 ISEE49 pKa = 4.25 SQKK52 pKa = 11.22 YY53 pKa = 8.02 MEE55 pKa = 5.69 DD56 pKa = 3.71 YY57 pKa = 10.88 IDD59 pKa = 6.21 AIDD62 pKa = 4.17 EE63 pKa = 4.57 DD64 pKa = 5.18 LSILQDD70 pKa = 4.07 DD71 pKa = 5.17 MYY73 pKa = 11.7 DD74 pKa = 4.31 DD75 pKa = 5.94 DD76 pKa = 6.11 YY77 pKa = 11.79 EE78 pKa = 4.52 LCEE81 pKa = 4.86 DD82 pKa = 4.09 EE83 pKa = 5.32 GDD85 pKa = 3.78 NFIQLKK91 pKa = 10.2 CSNCGDD97 pKa = 3.82 DD98 pKa = 4.34 VYY100 pKa = 11.62 VDD102 pKa = 3.74 KK103 pKa = 11.36 DD104 pKa = 3.26 IVKK107 pKa = 9.83 QKK109 pKa = 11.12 EE110 pKa = 4.28 KK111 pKa = 9.46 ITCPNCHH118 pKa = 6.1 NLIPTT123 pKa = 4.35
Molecular weight: 13.98 kDa
Isoelectric point according different methods:
IPC2.protein.svr19 3.732
IPC2_protein 3.795
IPC_protein 3.783
Toseland 3.567
ProMoST 3.948
Dawson 3.783
Bjellqvist 3.935
Wikipedia 3.719
Rodwell 3.617
Grimsley 3.478
Solomon 3.77
Lehninger 3.732
Nozaki 3.897
DTASelect 4.126
Thurlkill 3.63
EMBOSS 3.719
Sillero 3.91
Patrickios 1.875
IPC_peptide 3.77
IPC2_peptide 3.884
IPC2.peptide.svr19 3.825
Protein with the highest isoelectric point:
>tr|C6PVL6|C6PVL6_9CLOT Ethanolamine utilisation protein EutH OS=Clostridium carboxidivorans P7 OX=536227 GN=CcarbDRAFT_2833 PE=4 SV=1
MM1 pKa = 7.84 RR2 pKa = 11.84 IIKK5 pKa = 9.76 IFNGYY10 pKa = 9.18 FLILMIIQGLVLAFFDD26 pKa = 3.6 SRR28 pKa = 11.84 SFSRR32 pKa = 11.84 RR33 pKa = 11.84 NLRR36 pKa = 11.84 DD37 pKa = 3.13 VSKK40 pKa = 9.68 MAKK43 pKa = 9.62 FLGISFIIMSVCLYY57 pKa = 10.31 LVSTFTVV64 pKa = 3.21
Molecular weight: 7.45 kDa
Isoelectric point according different methods:
IPC2.protein.svr19 9.332
IPC2_protein 9.867
IPC_protein 10.657
Toseland 10.701
ProMoST 10.409
Dawson 10.818
Bjellqvist 10.526
Wikipedia 11.023
Rodwell 11.082
Grimsley 10.877
Solomon 10.921
Lehninger 10.891
Nozaki 10.687
DTASelect 10.526
Thurlkill 10.701
EMBOSS 11.096
Sillero 10.745
Patrickios 10.906
IPC_peptide 10.921
IPC2_peptide 9.604
IPC2.peptide.svr19 8.331
Peptides (in silico digests for buttom-up proteomics)
Below you can find
in silico digests of the whole proteome with Trypsin, Chymotrypsin, Trypsin+LysC, LysN, ArgC proteases suitable for different mass spec machines.
Try ESI
ChTry ESI
ArgC ESI
LysN ESI
TryLysC ESI
Try MALDI
ChTry MALDI
ArgC MALDI
LysN MALDI
TryLysC MALDI
Try LTQ
ChTry LTQ
ArgC LTQ
LysN LTQ
TryLysC LTQ
Try MSlow
ChTry MSlow
ArgC MSlow
LysN MSlow
TryLysC MSlow
Try MShigh
ChTry MShigh
ArgC MShigh
LysN MShigh
TryLysC MShigh
General Statistics
Number of major isoforms
Number of additional isoforms
Number of all proteins
Number of amino acids
Min. Seq. Length
Max. Seq. Length
Avg. Seq. Length
Avg. Mol. Weight
5458
0
5458
1581288
30
2252
289.7
32.61
Amino acid frequency
Ala
Cys
Asp
Glu
Phe
Gly
His
Ile
Lys
Leu
5.927 ± 0.036
1.347 ± 0.015
5.34 ± 0.029
7.077 ± 0.044
4.377 ± 0.029
6.375 ± 0.03
1.375 ± 0.013
9.711 ± 0.041
9.237 ± 0.035
8.879 ± 0.036
Met
Asn
Gln
Pro
Arg
Ser
Thr
Val
Trp
Tyr
2.757 ± 0.016
6.298 ± 0.035
2.838 ± 0.019
2.489 ± 0.015
3.115 ± 0.022
6.614 ± 0.032
4.916 ± 0.032
6.618 ± 0.032
0.705 ± 0.01
4.005 ± 0.024
Note: For amino acid frequency statistics the error has been estimated with the bootstraping (x100) at the protein level
Most of the basic statistics you can see at this page can be downloaded from this CSV file
For dipeptide frequency statistics click here