Clostridium carboxidivorans P7

Taxonomy: cellular organisms; Bacteria; Terrabacteria group; Firmicutes; Clostridia; Eubacteriales; Clostridiaceae; Clostridium; Clostridium carboxidivorans

Average proteome isoelectric point is 6.72

Get precalculated fractions of proteins

Acidic
pI < 6.8
6.8-7.4
pI > 7.4
Basic
    
All



Virtual 2D-PAGE plot for 5458 proteins (isoelectric point calculated using IPC2_protein)

Get csv file with sequences according to given criteria:
        -     Method 

     -  kDa    
                                                                                      

* You can choose from 21 different methods for calculating isoelectric point

Summary statistics related to proteome-wise predictions


    

Protein with the lowest isoelectric point:
>tr|C6PQT8|C6PQT8_9CLOT Uncharacterized protein OS=Clostridium carboxidivorans P7 OX=536227 GN=CcarbDRAFT_1155 PE=4 SV=1
MM1 pKa = 7.69SDD3 pKa = 3.8IISKK7 pKa = 9.85VAYY10 pKa = 10.7LNGLVDD16 pKa = 4.05GLEE19 pKa = 3.98IDD21 pKa = 3.72TTTKK25 pKa = 9.89EE26 pKa = 4.2GKK28 pKa = 9.48VLNEE32 pKa = 4.3IINVLKK38 pKa = 10.98VIAEE42 pKa = 4.16EE43 pKa = 3.79VDD45 pKa = 4.04EE46 pKa = 4.67ISEE49 pKa = 4.25SQKK52 pKa = 11.22YY53 pKa = 8.02MEE55 pKa = 5.69DD56 pKa = 3.71YY57 pKa = 10.88IDD59 pKa = 6.21AIDD62 pKa = 4.17EE63 pKa = 4.57DD64 pKa = 5.18LSILQDD70 pKa = 4.07DD71 pKa = 5.17MYY73 pKa = 11.7DD74 pKa = 4.31DD75 pKa = 5.94DD76 pKa = 6.11YY77 pKa = 11.79EE78 pKa = 4.52LCEE81 pKa = 4.86DD82 pKa = 4.09EE83 pKa = 5.32GDD85 pKa = 3.78NFIQLKK91 pKa = 10.2CSNCGDD97 pKa = 3.82DD98 pKa = 4.34VYY100 pKa = 11.62VDD102 pKa = 3.74KK103 pKa = 11.36DD104 pKa = 3.26IVKK107 pKa = 9.83QKK109 pKa = 11.12EE110 pKa = 4.28KK111 pKa = 9.46ITCPNCHH118 pKa = 6.1NLIPTT123 pKa = 4.35

Molecular weight:
13.98 kDa
Isoelectric point according different methods:






Protein with the highest isoelectric point:
>tr|C6PVL6|C6PVL6_9CLOT Ethanolamine utilisation protein EutH OS=Clostridium carboxidivorans P7 OX=536227 GN=CcarbDRAFT_2833 PE=4 SV=1
MM1 pKa = 7.84RR2 pKa = 11.84IIKK5 pKa = 9.76IFNGYY10 pKa = 9.18FLILMIIQGLVLAFFDD26 pKa = 3.6SRR28 pKa = 11.84SFSRR32 pKa = 11.84RR33 pKa = 11.84NLRR36 pKa = 11.84DD37 pKa = 3.13VSKK40 pKa = 9.68MAKK43 pKa = 9.62FLGISFIIMSVCLYY57 pKa = 10.31LVSTFTVV64 pKa = 3.21

Molecular weight:
7.45 kDa
Isoelectric point according different methods:






Peptides (in silico digests for buttom-up proteomics)

Below you can find in silico digests of the whole proteome with Trypsin, Chymotrypsin, Trypsin+LysC, LysN, ArgC proteases suitable for different mass spec machines.

Try
ESI
ChTry
ESI
ArgC
ESI
LysN
ESI
TryLysC
ESI

Try
MALDI
ChTry
MALDI
ArgC
MALDI
LysN
MALDI
TryLysC
MALDI

Try
LTQ
ChTry
LTQ
ArgC
LTQ
LysN
LTQ
TryLysC
LTQ

Try
MSlow
ChTry
MSlow
ArgC
MSlow
LysN
MSlow
TryLysC
MSlow

Try
MShigh
ChTry
MShigh
ArgC
MShigh
LysN
MShigh
TryLysC
MShigh

General Statistics

Number of major isoforms

Number of additional isoforms

Number of all proteins

Number of amino acids

Min. Seq. Length

Max. Seq. Length

Avg. Seq. Length

Avg. Mol. Weight

5458

0

5458

1581288

30

2252

289.7

32.61

Amino acid frequency

Ala

Cys

Asp

Glu

Phe

Gly

His

Ile

Lys

Leu

5.927 ± 0.036

1.347 ± 0.015

5.34 ± 0.029

7.077 ± 0.044

4.377 ± 0.029

6.375 ± 0.03

1.375 ± 0.013

9.711 ± 0.041

9.237 ± 0.035

8.879 ± 0.036

Met

Asn

Gln

Pro

Arg

Ser

Thr

Val

Trp

Tyr

2.757 ± 0.016

6.298 ± 0.035

2.838 ± 0.019

2.489 ± 0.015

3.115 ± 0.022

6.614 ± 0.032

4.916 ± 0.032

6.618 ± 0.032

0.705 ± 0.01

4.005 ± 0.024

Note: For amino acid frequency statistics the error has been estimated with the bootstraping (x100) at the protein level

Most of the basic statistics you can see at this page can be downloaded from this CSV file

For dipeptide frequency statistics click here
See this proteome in: uniprot_link
Proteome-pI is available under Creative Commons Attribution-NoDerivs license, for more details see here

Reference: Kozlowski LP. Proteome-pI 2.0: Proteome Isoelectric Point Database Update. Nucleic Acids Res. 2021, doi: 10.1093/nar/gkab944 Contact: Lukasz P. Kozlowski