Mycobacterium xenopi 3993
Average proteome isoelectric point is 7.44
Get precalculated fractions of proteins
Acidic
pI < 6.8
6.8-7.4
pI > 7.4
Basic
All
Note: above files contain also dissociation constants (pKa)
Virtual 2D-PAGE plot for 10123 proteins (isoelectric point calculated using IPC2_protein)
Get csv file with sequences according to given criteria:
* You can choose from 21 different methods for calculating isoelectric point
Summary statistics related to proteome-wise predictions
Protein with the lowest isoelectric point:
>tr|X7ZM67|X7ZM67_MYCXE Homoserine O-acetyltransferase domain protein OS=Mycobacterium xenopi 3993 OX=1299333 GN=I552_8982 PE=4 SV=1
MM1 pKa = 7.66 NDD3 pKa = 3.14 YY4 pKa = 11.38 KK5 pKa = 10.94 LFRR8 pKa = 11.84 CMQCGFEE15 pKa = 4.04 YY16 pKa = 10.84 DD17 pKa = 3.91 EE18 pKa = 4.49 ALGWPEE24 pKa = 5.22 DD25 pKa = 4.3 GIAPGTRR32 pKa = 11.84 WDD34 pKa = 5.68 DD35 pKa = 4.13 IPDD38 pKa = 3.72 DD39 pKa = 4.45 WSCPDD44 pKa = 3.66 CGAAKK49 pKa = 10.31 SDD51 pKa = 3.66 FEE53 pKa = 4.31 MVEE56 pKa = 4.21 VARR59 pKa = 11.84 SS60 pKa = 3.19
Molecular weight: 6.84 kDa
Isoelectric point according different methods:
IPC2.protein.svr19 3.749
IPC2_protein 3.872
IPC_protein 3.795
Toseland 3.592
ProMoST 3.986
Dawson 3.808
Bjellqvist 3.961
Wikipedia 3.757
Rodwell 3.63
Grimsley 3.503
Solomon 3.783
Lehninger 3.732
Nozaki 3.935
DTASelect 4.151
Thurlkill 3.668
EMBOSS 3.77
Sillero 3.923
Patrickios 1.901
IPC_peptide 3.77
IPC2_peptide 3.897
IPC2.peptide.svr19 3.83
Protein with the highest isoelectric point:
>tr|X8BKP3|X8BKP3_MYCXE Uncharacterized protein OS=Mycobacterium xenopi 3993 OX=1299333 GN=I552_8375 PE=4 SV=1
MM1 pKa = 7.69 AKK3 pKa = 10.06 GKK5 pKa = 8.69 RR6 pKa = 11.84 TFQPNNRR13 pKa = 11.84 RR14 pKa = 11.84 RR15 pKa = 11.84 ARR17 pKa = 11.84 VHH19 pKa = 5.99 GFRR22 pKa = 11.84 LRR24 pKa = 11.84 MRR26 pKa = 11.84 TRR28 pKa = 11.84 AGRR31 pKa = 11.84 AIVSNRR37 pKa = 11.84 RR38 pKa = 11.84 RR39 pKa = 11.84 KK40 pKa = 9.46 GRR42 pKa = 11.84 RR43 pKa = 11.84 ALTAA47 pKa = 3.88
Molecular weight: 5.59 kDa
Isoelectric point according different methods:
IPC2.protein.svr19 9.523
IPC2_protein 11.374
IPC_protein 12.983
Toseland 13.144
ProMoST 13.642
Dawson 13.144
Bjellqvist 13.144
Wikipedia 13.627
Rodwell 12.735
Grimsley 13.188
Solomon 13.642
Lehninger 13.554
Nozaki 13.144
DTASelect 13.144
Thurlkill 13.144
EMBOSS 13.642
Sillero 13.144
Patrickios 12.457
IPC_peptide 13.656
IPC2_peptide 12.632
IPC2.peptide.svr19 9.285
Peptides (in silico digests for buttom-up proteomics)
Below you can find
in silico digests of the whole proteome with Trypsin, Chymotrypsin, Trypsin+LysC, LysN, ArgC proteases suitable for different mass spec machines.
Try ESI
ChTry ESI
ArgC ESI
LysN ESI
TryLysC ESI
Try MALDI
ChTry MALDI
ArgC MALDI
LysN MALDI
TryLysC MALDI
Try LTQ
ChTry LTQ
ArgC LTQ
LysN LTQ
TryLysC LTQ
Try MSlow
ChTry MSlow
ArgC MSlow
LysN MSlow
TryLysC MSlow
Try MShigh
ChTry MShigh
ArgC MShigh
LysN MShigh
TryLysC MShigh
General Statistics
Number of major isoforms
Number of additional isoforms
Number of all proteins
Number of amino acids
Min. Seq. Length
Max. Seq. Length
Avg. Seq. Length
Avg. Mol. Weight
10123
0
10123
1523496
37
927
150.5
16.32
Amino acid frequency
Ala
Cys
Asp
Glu
Phe
Gly
His
Ile
Lys
Leu
12.758 ± 0.037
1.585 ± 0.015
5.463 ± 0.023
4.467 ± 0.027
2.686 ± 0.018
8.44 ± 0.025
2.437 ± 0.014
3.87 ± 0.019
2.116 ± 0.017
8.853 ± 0.031
Met
Asn
Gln
Pro
Arg
Ser
Thr
Val
Trp
Tyr
2.233 ± 0.013
2.117 ± 0.014
6.572 ± 0.031
3.012 ± 0.018
9.543 ± 0.036
6.207 ± 0.03
6.134 ± 0.021
7.718 ± 0.028
1.859 ± 0.014
1.931 ± 0.014
Note: For amino acid frequency statistics the error has been estimated with the bootstraping (x100) at the protein level
Most of the basic statistics you can see at this page can be downloaded from this CSV file
For dipeptide frequency statistics click here