Tomato yellow dwarf disease associated satellite DNA beta-[Kochi]
Average proteome isoelectric point is 5.25
Get precalculated fractions of proteins
Acidic
pI < 6.8
6.8-7.4
pI > 7.4
Basic
All
Note: above files contain also dissociation constants (pKa)
Virtual 2D-PAGE plot for 1 proteins (isoelectric point calculated using IPC2_protein)
Get csv file with sequences according to given criteria:
* You can choose from 21 different methods for calculating isoelectric point
Summary statistics related to proteome-wise predictions
Protein with the lowest isoelectric point:
>tr|A7M6K4|A7M6K4_9VIRU BetaC1 protein OS=Tomato yellow dwarf disease associated satellite DNA beta-[Kochi] OX=427315 GN=betaC1 PE=4 SV=1
MM1 pKa = 7.72 TITYY5 pKa = 10.78 NNGKK9 pKa = 9.48 GIKK12 pKa = 10.02 FIVDD16 pKa = 3.31 VRR18 pKa = 11.84 LHH20 pKa = 5.73 QLLKK24 pKa = 11.02 VIVQVYY30 pKa = 7.05 STNKK34 pKa = 9.41 PVLTGFKK41 pKa = 10.2 CHH43 pKa = 6.53 IPYY46 pKa = 9.57 TYY48 pKa = 11.1 VQMVPPFDD56 pKa = 4.28 FNGAEE61 pKa = 3.91 EE62 pKa = 5.24 LIRR65 pKa = 11.84 EE66 pKa = 4.46 TLEE69 pKa = 4.77 LMYY72 pKa = 10.58 EE73 pKa = 4.44 DD74 pKa = 5.31 SDD76 pKa = 3.44 ISNFKK81 pKa = 9.67 QEE83 pKa = 4.06 EE84 pKa = 4.41 MIDD87 pKa = 4.07 SIDD90 pKa = 3.54 MVMMHH95 pKa = 7.34 WLGHH99 pKa = 5.57 MGVDD103 pKa = 3.18 TVDD106 pKa = 3.61 RR107 pKa = 11.84 YY108 pKa = 8.71 TIRR111 pKa = 11.84 CRR113 pKa = 11.84 NTVV116 pKa = 2.75
Molecular weight: 13.58 kDa
Isoelectric point according different methods:
IPC2.protein.svr19 5.25
IPC2_protein 5.334
IPC_protein 5.258
Toseland 5.461
ProMoST 5.512
Dawson 5.385
Bjellqvist 5.448
Wikipedia 5.334
Rodwell 5.334
Grimsley 5.512
Solomon 5.385
Lehninger 5.359
Nozaki 5.575
DTASelect 5.766
Thurlkill 5.588
EMBOSS 5.563
Sillero 5.664
Patrickios 3.719
IPC_peptide 5.397
IPC2_peptide 5.664
IPC2.peptide.svr19 5.695
Protein with the highest isoelectric point:
>tr|A7M6K4|A7M6K4_9VIRU BetaC1 protein OS=Tomato yellow dwarf disease associated satellite DNA beta-[Kochi] OX=427315 GN=betaC1 PE=4 SV=1
MM1 pKa = 7.72 TITYY5 pKa = 10.78 NNGKK9 pKa = 9.48 GIKK12 pKa = 10.02 FIVDD16 pKa = 3.31 VRR18 pKa = 11.84 LHH20 pKa = 5.73 QLLKK24 pKa = 11.02 VIVQVYY30 pKa = 7.05 STNKK34 pKa = 9.41 PVLTGFKK41 pKa = 10.2 CHH43 pKa = 6.53 IPYY46 pKa = 9.57 TYY48 pKa = 11.1 VQMVPPFDD56 pKa = 4.28 FNGAEE61 pKa = 3.91 EE62 pKa = 5.24 LIRR65 pKa = 11.84 EE66 pKa = 4.46 TLEE69 pKa = 4.77 LMYY72 pKa = 10.58 EE73 pKa = 4.44 DD74 pKa = 5.31 SDD76 pKa = 3.44 ISNFKK81 pKa = 9.67 QEE83 pKa = 4.06 EE84 pKa = 4.41 MIDD87 pKa = 4.07 SIDD90 pKa = 3.54 MVMMHH95 pKa = 7.34 WLGHH99 pKa = 5.57 MGVDD103 pKa = 3.18 TVDD106 pKa = 3.61 RR107 pKa = 11.84 YY108 pKa = 8.71 TIRR111 pKa = 11.84 CRR113 pKa = 11.84 NTVV116 pKa = 2.75
Molecular weight: 13.58 kDa
Isoelectric point according different methods:
IPC2.protein.svr19 5.25
IPC2_protein 5.334
IPC_protein 5.258
Toseland 5.461
ProMoST 5.512
Dawson 5.385
Bjellqvist 5.448
Wikipedia 5.334
Rodwell 5.334
Grimsley 5.512
Solomon 5.385
Lehninger 5.359
Nozaki 5.575
DTASelect 5.766
Thurlkill 5.588
EMBOSS 5.563
Sillero 5.664
Patrickios 3.719
IPC_peptide 5.397
IPC2_peptide 5.664
IPC2.peptide.svr19 5.695
Peptides (in silico digests for buttom-up proteomics)
Below you can find
in silico digests of the whole proteome with Trypsin, Chymotrypsin, Trypsin+LysC, LysN, ArgC proteases suitable for different mass spec machines.
Try ESI
ChTry ESI
ArgC ESI
LysN ESI
TryLysC ESI
Try MALDI
ChTry MALDI
ArgC MALDI
LysN MALDI
TryLysC MALDI
Try LTQ
ChTry LTQ
ArgC LTQ
LysN LTQ
TryLysC LTQ
Try MSlow
ChTry MSlow
ArgC MSlow
LysN MSlow
TryLysC MSlow
Try MShigh
ChTry MShigh
ArgC MShigh
LysN MShigh
TryLysC MShigh
General Statistics
Number of major isoforms
Number of additional isoforms
Number of all proteins
Number of amino acids
Min. Seq. Length
Max. Seq. Length
Avg. Seq. Length
Avg. Mol. Weight
1
0
1
116
116
116
116.0
13.58
Amino acid frequency
Ala
Cys
Asp
Glu
Phe
Gly
His
Ile
Lys
Leu
0.862 ± 0.0
1.724 ± 0.0
6.897 ± 0.0
6.034 ± 0.0
4.31 ± 0.0
5.172 ± 0.0
3.448 ± 0.0
8.621 ± 0.0
5.172 ± 0.0
6.897 ± 0.0
Met
Asn
Gln
Pro
Arg
Ser
Thr
Val
Trp
Tyr
6.897 ± 0.0
5.172 ± 0.0
3.448 ± 0.0
3.448 ± 0.0
4.31 ± 0.0
3.448 ± 0.0
7.759 ± 0.0
10.345 ± 0.0
0.862 ± 0.0
5.172 ± 0.0
Note: For amino acid frequency statistics the error has been estimated with the bootstraping (x100) at the protein level
Most of the basic statistics you can see at this page can be downloaded from this CSV file
For dipeptide frequency statistics click here