Mint virus 1
Average proteome isoelectric point is 6.68
Get precalculated fractions of proteins
Acidic
pI < 6.8
6.8-7.4
pI > 7.4
Basic
All
Note: above files contain also dissociation constants (pKa)
Virtual 2D-PAGE plot for 9 proteins (isoelectric point calculated using IPC2_protein)
Get csv file with sequences according to given criteria:
* You can choose from 21 different methods for calculating isoelectric point
Summary statistics related to proteome-wise predictions
Protein with the lowest isoelectric point:
>tr|Q5G7G1|Q5G7G1_9CLOS Coat protein OS=Mint virus 1 OX=300740 PE=4 SV=1
MM1 pKa = 7.7 SEE3 pKa = 3.87 VSFSIRR9 pKa = 11.84 PIADD13 pKa = 3.2 GDD15 pKa = 4.06 TNTIVIIRR23 pKa = 11.84 DD24 pKa = 3.48 KK25 pKa = 10.86 FGHH28 pKa = 5.79 SVLNFKK34 pKa = 10.78 SPAGDD39 pKa = 3.6 DD40 pKa = 3.47 SLALVEE46 pKa = 4.35 EE47 pKa = 4.48 SCIISRR53 pKa = 11.84 SDD55 pKa = 3.5 FEE57 pKa = 5.46 SVASCDD63 pKa = 4.13 DD64 pKa = 3.77 SFFLAGLNSLEE75 pKa = 4.24 DD76 pKa = 3.57 ALNNFVLHH84 pKa = 6.19 SPRR87 pKa = 11.84 SLTATSEE94 pKa = 4.39 SLSSLEE100 pKa = 4.32 YY101 pKa = 9.97 IDD103 pKa = 6.04 FVLDD107 pKa = 4.36 PIMLGIIFRR116 pKa = 11.84 FNLKK120 pKa = 10.07 RR121 pKa = 11.84 RR122 pKa = 11.84 INNDD126 pKa = 2.89 SVSLFRR132 pKa = 11.84 DD133 pKa = 3.31 SLISLNGGWYY143 pKa = 10.5 NGTLYY148 pKa = 11.17 DD149 pKa = 5.79 DD150 pKa = 3.81 MFHH153 pKa = 6.43 YY154 pKa = 10.78 LSNFFKK160 pKa = 10.53 QKK162 pKa = 10.34 KK163 pKa = 9.35 GFTIHH168 pKa = 6.45 SFEE171 pKa = 5.49 LIKK174 pKa = 10.82 QNEE177 pKa = 4.46 VVFPSRR183 pKa = 11.84 VLRR186 pKa = 11.84 VSGDD190 pKa = 3.42 RR191 pKa = 11.84 GHH193 pKa = 6.43 QPIDD197 pKa = 3.12
Molecular weight: 22.17 kDa
Isoelectric point according different methods:
IPC2.protein.svr19 4.953
IPC2_protein 4.952
IPC_protein 4.889
Toseland 4.8
ProMoST 4.94
Dawson 4.902
Bjellqvist 5.054
Wikipedia 4.813
Rodwell 4.787
Grimsley 4.724
Solomon 4.902
Lehninger 4.863
Nozaki 5.029
DTASelect 5.245
Thurlkill 4.825
EMBOSS 4.851
Sillero 5.08
Patrickios 4.164
IPC_peptide 4.902
IPC2_peptide 5.067
IPC2.peptide.svr19 5.038
Protein with the highest isoelectric point:
>tr|Q5G7G6|Q5G7G6_9CLOS Polymerase (Fragment) OS=Mint virus 1 OX=300740 PE=4 SV=1
MM1 pKa = 8.04 DD2 pKa = 4.1 CTLRR6 pKa = 11.84 AYY8 pKa = 10.31 FYY10 pKa = 11.39 LLLGWIIVCFSFTLGFVVYY29 pKa = 10.19 KK30 pKa = 10.19 LVRR33 pKa = 11.84 TCSNVYY39 pKa = 10.71 GDD41 pKa = 5.02 IIDD44 pKa = 3.86 TSVVGTSRR52 pKa = 11.84 RR53 pKa = 11.84 IDD55 pKa = 3.11 IEE57 pKa = 3.88 NRR59 pKa = 11.84 GNSSARR65 pKa = 11.84 VV66 pKa = 3.35
Molecular weight: 7.49 kDa
Isoelectric point according different methods:
IPC2.protein.svr19 7.827
IPC2_protein 7.6
IPC_protein 7.79
Toseland 7.146
ProMoST 8.053
Dawson 8.244
Bjellqvist 8.595
Wikipedia 8.17
Rodwell 8.229
Grimsley 7.0
Solomon 8.492
Lehninger 8.507
Nozaki 8.785
DTASelect 8.331
Thurlkill 8.361
EMBOSS 8.478
Sillero 8.712
Patrickios 3.503
IPC_peptide 8.478
IPC2_peptide 8.317
IPC2.peptide.svr19 8.385
Peptides (in silico digests for buttom-up proteomics)
Below you can find
in silico digests of the whole proteome with Trypsin, Chymotrypsin, Trypsin+LysC, LysN, ArgC proteases suitable for different mass spec machines.
Try ESI
ChTry ESI
ArgC ESI
LysN ESI
TryLysC ESI
Try MALDI
ChTry MALDI
ArgC MALDI
LysN MALDI
TryLysC MALDI
Try LTQ
ChTry LTQ
ArgC LTQ
LysN LTQ
TryLysC LTQ
Try MSlow
ChTry MSlow
ArgC MSlow
LysN MSlow
TryLysC MSlow
Try MShigh
ChTry MShigh
ArgC MShigh
LysN MShigh
TryLysC MShigh
General Statistics
Number of major isoforms
Number of additional isoforms
Number of all proteins
Number of amino acids
Min. Seq. Length
Max. Seq. Length
Avg. Seq. Length
Avg. Mol. Weight
9
0
9
4981
66
2511
553.4
62.31
Amino acid frequency
Ala
Cys
Asp
Glu
Phe
Gly
His
Ile
Lys
Leu
5.682 ± 0.555
2.53 ± 0.181
5.983 ± 0.353
5.722 ± 0.259
6.204 ± 0.423
5.14 ± 0.235
2.148 ± 0.231
5.441 ± 0.313
5.682 ± 0.422
8.894 ± 0.649
Met
Asn
Gln
Pro
Arg
Ser
Thr
Val
Trp
Tyr
1.927 ± 0.145
4.357 ± 0.325
3.714 ± 0.293
1.927 ± 0.173
6.545 ± 0.45
10.359 ± 0.76
5.32 ± 0.314
8.532 ± 0.657
0.482 ± 0.185
3.413 ± 0.199
Note: For amino acid frequency statistics the error has been estimated with the bootstraping (x100) at the protein level
Most of the basic statistics you can see at this page can be downloaded from this CSV file
For dipeptide frequency statistics click here