Gordonia phage WhoseManz
Average proteome isoelectric point is 6.09
Get precalculated fractions of proteins
Acidic
pI < 6.8
6.8-7.4
pI > 7.4
Basic
All
Note: above files contain also dissociation constants (pKa)
Virtual 2D-PAGE plot for 92 proteins (isoelectric point calculated using IPC2_protein)
Get csv file with sequences according to given criteria:
* You can choose from 21 different methods for calculating isoelectric point
Summary statistics related to proteome-wise predictions
Protein with the lowest isoelectric point:
>tr|A0A410TBG9|A0A410TBG9_9CAUD Tail assembly chaperone OS=Gordonia phage WhoseManz OX=2507855 GN=39 PE=4 SV=1
MM1 pKa = 7.5 TWTQPEE7 pKa = 4.43 LGDD10 pKa = 3.89 LVAEE14 pKa = 4.24 AAEE17 pKa = 4.9 LYY19 pKa = 10.81 DD20 pKa = 4.33 GFNSDD25 pKa = 3.7 FPEE28 pKa = 4.58 GAVYY32 pKa = 10.23 PDD34 pKa = 3.6 IEE36 pKa = 4.31 KK37 pKa = 11.02 LLAKK41 pKa = 9.79 VLRR44 pKa = 11.84 PLVEE48 pKa = 4.36 KK49 pKa = 10.49 DD50 pKa = 3.11 SHH52 pKa = 7.33 IGNFVVSDD60 pKa = 3.83 YY61 pKa = 11.31 DD62 pKa = 3.32 QDD64 pKa = 5.62 SIDD67 pKa = 3.84 EE68 pKa = 4.24 DD69 pKa = 3.66 TGEE72 pKa = 4.16 EE73 pKa = 4.11 VAGPFIEE80 pKa = 3.71 IHH82 pKa = 5.85 RR83 pKa = 11.84 RR84 pKa = 11.84 GGEE87 pKa = 3.81 YY88 pKa = 10.75 DD89 pKa = 3.86 PDD91 pKa = 3.99 DD92 pKa = 3.9 FSYY95 pKa = 10.78 YY96 pKa = 10.12 PNVEE100 pKa = 3.78 VLFWGKK106 pKa = 9.89 SRR108 pKa = 11.84 PVANDD113 pKa = 3.28 TAHH116 pKa = 7.3 KK117 pKa = 9.63 GTILLLGCSGAVIDD131 pKa = 5.7 GVQIDD136 pKa = 4.62 LLEE139 pKa = 4.76 DD140 pKa = 3.22 ATGDD144 pKa = 3.66 EE145 pKa = 4.84 EE146 pKa = 4.93 VRR148 pKa = 11.84 QNNFDD153 pKa = 3.81 DD154 pKa = 4.14 RR155 pKa = 11.84 CVTRR159 pKa = 11.84 QFRR162 pKa = 11.84 VGYY165 pKa = 9.99 RR166 pKa = 11.84 PTYY169 pKa = 10.09 PDD171 pKa = 2.9
Molecular weight: 19.17 kDa
Isoelectric point according different methods:
IPC2.protein.svr19 4.158
IPC2_protein 4.05
IPC_protein 4.037
Toseland 3.834
ProMoST 4.151
Dawson 4.024
Bjellqvist 4.202
Wikipedia 3.935
Rodwell 3.872
Grimsley 3.745
Solomon 4.012
Lehninger 3.973
Nozaki 4.126
DTASelect 4.355
Thurlkill 3.872
EMBOSS 3.948
Sillero 4.164
Patrickios 3.389
IPC_peptide 4.012
IPC2_peptide 4.139
IPC2.peptide.svr19 4.071
Protein with the highest isoelectric point:
>tr|A0A410TBC2|A0A410TBC2_9CAUD Uncharacterized protein OS=Gordonia phage WhoseManz OX=2507855 GN=22 PE=4 SV=1
MM1 pKa = 6.97 AQRR4 pKa = 11.84 RR5 pKa = 11.84 RR6 pKa = 11.84 RR7 pKa = 11.84 GGALNTRR14 pKa = 11.84 KK15 pKa = 9.95 KK16 pKa = 10.91 GKK18 pKa = 10.05 AKK20 pKa = 10.66 NGAKK24 pKa = 9.07 WGHH27 pKa = 5.7 GFVPKK32 pKa = 10.29 NAAARR37 pKa = 11.84 KK38 pKa = 9.03 LKK40 pKa = 10.66 KK41 pKa = 10.41 KK42 pKa = 10.3 LDD44 pKa = 3.9 RR45 pKa = 11.84 NGKK48 pKa = 8.0 RR49 pKa = 11.84 RR50 pKa = 11.84 KK51 pKa = 8.72 RR52 pKa = 11.84 TAQGYY57 pKa = 6.78 PKK59 pKa = 10.41 RR60 pKa = 4.1
Molecular weight: 6.86 kDa
Isoelectric point according different methods:
IPC2.protein.svr19 9.344
IPC2_protein 10.891
IPC_protein 12.281
Toseland 12.457
ProMoST 12.939
Dawson 12.457
Bjellqvist 12.442
Wikipedia 12.925
Rodwell 12.34
Grimsley 12.501
Solomon 12.939
Lehninger 12.837
Nozaki 12.457
DTASelect 12.442
Thurlkill 12.457
EMBOSS 12.939
Sillero 12.457
Patrickios 12.062
IPC_peptide 12.939
IPC2_peptide 11.915
IPC2.peptide.svr19 8.994
Peptides (in silico digests for buttom-up proteomics)
Below you can find
in silico digests of the whole proteome with Trypsin, Chymotrypsin, Trypsin+LysC, LysN, ArgC proteases suitable for different mass spec machines.
Try ESI
ChTry ESI
ArgC ESI
LysN ESI
TryLysC ESI
Try MALDI
ChTry MALDI
ArgC MALDI
LysN MALDI
TryLysC MALDI
Try LTQ
ChTry LTQ
ArgC LTQ
LysN LTQ
TryLysC LTQ
Try MSlow
ChTry MSlow
ArgC MSlow
LysN MSlow
TryLysC MSlow
Try MShigh
ChTry MShigh
ArgC MShigh
LysN MShigh
TryLysC MShigh
General Statistics
Number of major isoforms
Number of additional isoforms
Number of all proteins
Number of amino acids
Min. Seq. Length
Max. Seq. Length
Avg. Seq. Length
Avg. Mol. Weight
92
0
92
20452
39
1799
222.3
24.44
Amino acid frequency
Ala
Cys
Asp
Glu
Phe
Gly
His
Ile
Lys
Leu
10.415 ± 0.494
0.865 ± 0.117
7.232 ± 0.323
6.42 ± 0.276
3.056 ± 0.157
8.581 ± 0.378
2.005 ± 0.156
4.665 ± 0.211
4.161 ± 0.255
7.569 ± 0.267
Met
Asn
Gln
Pro
Arg
Ser
Thr
Val
Trp
Tyr
2.288 ± 0.119
3.296 ± 0.174
5.53 ± 0.198
3.203 ± 0.141
7.285 ± 0.38
5.427 ± 0.192
6.381 ± 0.219
7.144 ± 0.212
1.917 ± 0.127
2.562 ± 0.18
Note: For amino acid frequency statistics the error has been estimated with the bootstraping (x100) at the protein level
Most of the basic statistics you can see at this page can be downloaded from this CSV file
For dipeptide frequency statistics click here