Aspergillus bertholletiae

Taxonomy: cellular organisms; Eukaryota; Opisthokonta; Fungi; Dikarya; Ascomycota; saccharomyceta; Pezizomycotina; leotiomyceta; Eurotiomycetes; Eurotiomycetidae; Eurotiales; Aspergillaceae; Aspergillus

Average proteome isoelectric point is 6.49

Get precalculated fractions of proteins

Acidic
pI < 6.8
6.8-7.4
pI > 7.4
Basic
    
All



Virtual 2D-PAGE plot for 12944 proteins (isoelectric point calculated using IPC2_protein)

Get csv file with sequences according to given criteria:
        -     Method 

     -  kDa    
                                                                                      

* You can choose from 21 different methods for calculating isoelectric point

Summary statistics related to proteome-wise predictions


    
Protein with the lowest isoelectric point:
>tr|A0A5N7BLQ2|A0A5N7BLQ2_9EURO Uncharacterized protein OS=Aspergillus bertholletiae OX=1226010 GN=BDV26DRAFT_288237 PE=4 SV=1
MM1 pKa = 7.22ATSLLTIISTLLLIFGARR19 pKa = 11.84AFTLDD24 pKa = 3.58PVGLVYY30 pKa = 10.78APASVDD36 pKa = 2.79KK37 pKa = 10.64RR38 pKa = 11.84QAFTGTLGGSAPEE51 pKa = 4.08VTNTGDD57 pKa = 3.22SQRR60 pKa = 11.84PYY62 pKa = 11.06GVDD65 pKa = 3.0GDD67 pKa = 4.21TFTDD71 pKa = 3.67YY72 pKa = 11.13KK73 pKa = 10.75SAASRR78 pKa = 11.84SCNNQFDD85 pKa = 4.27SCQKK89 pKa = 10.07IANTDD94 pKa = 3.35QSSSFSLQDD103 pKa = 3.98CQDD106 pKa = 3.41QLNTCMSTVSSTSVALQGATIASAPVASSSTTDD139 pKa = 2.65ATTAVTAQFAQTTIPYY155 pKa = 10.06DD156 pKa = 3.5SEE158 pKa = 4.47FDD160 pKa = 4.03LVCDD164 pKa = 4.42LL165 pKa = 5.16

Molecular weight: 17.21 kDa
Isoelectric point according different methods:





Protein with the highest isoelectric point:
>tr|A0A5N7BKH5|A0A5N7BKH5_9EURO Transcription factor IIIC subunit delta N-term-domain-containing protein OS=Aspergillus bertholletiae OX=1226010 GN=BDV26DRAFT_288580 PE=4 SV=1
MM1 pKa = 7.88PSHH4 pKa = 6.91KK5 pKa = 10.39SFRR8 pKa = 11.84TKK10 pKa = 10.45QKK12 pKa = 9.84LAKK15 pKa = 9.55AQKK18 pKa = 8.59QNRR21 pKa = 11.84PIPQWIRR28 pKa = 11.84LRR30 pKa = 11.84TGNTIRR36 pKa = 11.84YY37 pKa = 5.79NAKK40 pKa = 8.89RR41 pKa = 11.84RR42 pKa = 11.84HH43 pKa = 4.14WRR45 pKa = 11.84KK46 pKa = 7.51TRR48 pKa = 11.84LGLL51 pKa = 4.09

Molecular weight: 6.25 kDa
Isoelectric point according different methods:





Peptides (in silico digests for buttom-up proteomics)

Below you can find in silico digests of the whole proteome with Trypsin, Chymotrypsin, Trypsin+LysC, LysN, ArgC proteases suitable for different mass spec machines.

Try
ESI
ChTry
ESI
ArgC
ESI
LysN
ESI
TryLysC
ESI

Try
MALDI
ChTry
MALDI
ArgC
MALDI
LysN
MALDI
TryLysC
MALDI

Try
LTQ
ChTry
LTQ
ArgC
LTQ
LysN
LTQ
TryLysC
LTQ

Try
MSlow
ChTry
MSlow
ArgC
MSlow
LysN
MSlow
TryLysC
MSlow

Try
MShigh
ChTry
MShigh
ArgC
MShigh
LysN
MShigh
TryLysC
MShigh

General Statistics

Number of major isoforms

Number of additional isoforms

Number of all proteins

Number of amino acids

Min. Seq. Length

Max. Seq. Length

Avg. Seq. Length

Avg. Mol. Weight

12944

0

12944

5951370

49

7734

459.8

51.01

Amino acid frequency

Ala

Cys

Asp

Glu

Phe

Gly

His

Ile

Lys

Leu

8.24 ± 0.018

1.377 ± 0.009

5.534 ± 0.013

5.96 ± 0.021

3.857 ± 0.013

6.786 ± 0.019

2.479 ± 0.01

5.193 ± 0.016

4.528 ± 0.018

9.258 ± 0.024

Met

Asn

Gln

Pro

Arg

Ser

Thr

Val

Trp

Tyr

2.187 ± 0.008

3.691 ± 0.011

5.902 ± 0.022

4.046 ± 0.016

6.021 ± 0.019

8.313 ± 0.023

5.919 ± 0.012

6.219 ± 0.017

1.532 ± 0.009

2.959 ± 0.011
Note: For amino acid frequency statistics the error has been estimated with the bootstraping (x100) at the protein level

Most of the basic statistics you can see at this page can be downloaded from this CSV file

For dipeptide frequency statistics click here
See this proteome in: uniprot_link
Proteome-pI is available under Creative Commons Attribution-NoDerivs license, for more details see here

Reference: Kozlowski LP. Proteome-pI 2.0: Proteome Isoelectric Point Database Update. Nucleic Acids Res. 2021, doi: 10.1093/nar/gkab944 Contact: Lukasz P. Kozlowski