Streptococcus phage 11865
Average proteome isoelectric point is 6.2
Get precalculated fractions of proteins
Acidic
pI < 6.8
6.8-7.4
pI > 7.4
Basic
All
Note: above files contain also dissociation constants (pKa)
Virtual 2D-PAGE plot for 48 proteins (isoelectric point calculated using IPC2_protein)
Get csv file with sequences according to given criteria:
* You can choose from 21 different methods for calculating isoelectric point
Summary statistics related to proteome-wise predictions
Protein with the lowest isoelectric point:
>tr|E8ZDQ8|E8ZDQ8_9CAUD Phage protein OS=Streptococcus phage 11865 OX=870473 PE=4 SV=1
MM1 pKa = 7.92 KK2 pKa = 10.19 EE3 pKa = 3.58 ILEE6 pKa = 4.43 EE7 pKa = 3.94 NLEE10 pKa = 4.11 ASHH13 pKa = 7.68 DD14 pKa = 4.04 YY15 pKa = 10.94 TSVLVVSLDD24 pKa = 3.24 KK25 pKa = 11.36 DD26 pKa = 3.74 GEE28 pKa = 4.37 INLGYY33 pKa = 10.32 SWDD36 pKa = 3.9 SSLQALGMLDD46 pKa = 3.33 VAKK49 pKa = 10.77 NYY51 pKa = 9.72 ILNVINN57 pKa = 4.36
Molecular weight: 6.37 kDa
Isoelectric point according different methods:
IPC2.protein.svr19 4.13
IPC2_protein 4.113
IPC_protein 3.961
Toseland 3.783
ProMoST 4.126
Dawson 3.948
Bjellqvist 4.101
Wikipedia 3.872
Rodwell 3.795
Grimsley 3.706
Solomon 3.923
Lehninger 3.872
Nozaki 4.075
DTASelect 4.24
Thurlkill 3.846
EMBOSS 3.884
Sillero 4.075
Patrickios 3.198
IPC_peptide 3.923
IPC2_peptide 4.062
IPC2.peptide.svr19 3.983
Protein with the highest isoelectric point:
>tr|E8ZDQ1|E8ZDQ1_9CAUD Phage protein OS=Streptococcus phage 11865 OX=870473 PE=4 SV=1
MM1 pKa = 7.49 QGILRR6 pKa = 11.84 EE7 pKa = 4.05 KK8 pKa = 9.96 GQGIAEE14 pKa = 4.08 RR15 pKa = 11.84 AGEE18 pKa = 4.24 GFEE21 pKa = 4.43 LTVSPGQKK29 pKa = 9.27 RR30 pKa = 11.84 ANAKK34 pKa = 10.11 ISTTDD39 pKa = 3.02 IKK41 pKa = 11.66 SMARR45 pKa = 11.84 NKK47 pKa = 9.84 KK48 pKa = 9.99 HH49 pKa = 6.79 NILLKK54 pKa = 10.84 AMRR57 pKa = 4.66
Molecular weight: 6.28 kDa
Isoelectric point according different methods:
IPC2.protein.svr19 9.184
IPC2_protein 9.633
IPC_protein 9.882
Toseland 11.023
ProMoST 11.008
Dawson 11.052
Bjellqvist 10.672
Wikipedia 11.199
Rodwell 11.506
Grimsley 11.067
Solomon 11.184
Lehninger 11.169
Nozaki 10.979
DTASelect 10.672
Thurlkill 10.979
EMBOSS 11.418
Sillero 10.994
Patrickios 11.272
IPC_peptide 11.199
IPC2_peptide 8.975
IPC2.peptide.svr19 8.766
Peptides (in silico digests for buttom-up proteomics)
Below you can find
in silico digests of the whole proteome with Trypsin, Chymotrypsin, Trypsin+LysC, LysN, ArgC proteases suitable for different mass spec machines.
Try ESI
ChTry ESI
ArgC ESI
LysN ESI
TryLysC ESI
Try MALDI
ChTry MALDI
ArgC MALDI
LysN MALDI
TryLysC MALDI
Try LTQ
ChTry LTQ
ArgC LTQ
LysN LTQ
TryLysC LTQ
Try MSlow
ChTry MSlow
ArgC MSlow
LysN MSlow
TryLysC MSlow
Try MShigh
ChTry MShigh
ArgC MShigh
LysN MShigh
TryLysC MShigh
General Statistics
Number of major isoforms
Number of additional isoforms
Number of all proteins
Number of amino acids
Min. Seq. Length
Max. Seq. Length
Avg. Seq. Length
Avg. Mol. Weight
48
0
48
9971
38
1421
207.7
23.53
Amino acid frequency
Ala
Cys
Asp
Glu
Phe
Gly
His
Ile
Lys
Leu
6.73 ± 0.418
0.652 ± 0.111
5.897 ± 0.282
7.632 ± 0.509
4.313 ± 0.254
7.101 ± 0.474
1.524 ± 0.165
6.92 ± 0.382
8.093 ± 0.433
8.194 ± 0.329
Met
Asn
Gln
Pro
Arg
Ser
Thr
Val
Trp
Tyr
2.467 ± 0.197
5.596 ± 0.331
2.708 ± 0.286
3.961 ± 0.18
4.684 ± 0.322
6.429 ± 0.373
5.626 ± 0.306
6.027 ± 0.283
1.494 ± 0.177
3.951 ± 0.245
Note: For amino acid frequency statistics the error has been estimated with the bootstraping (x100) at the protein level
Most of the basic statistics you can see at this page can be downloaded from this CSV file
For dipeptide frequency statistics click here