Arthrobacter phage Beagle
Average proteome isoelectric point is 6.38
Get precalculated fractions of proteins
Acidic
pI < 6.8
6.8-7.4
pI > 7.4
Basic
All
Note: above files contain also dissociation constants (pKa)
Virtual 2D-PAGE plot for 131 proteins (isoelectric point calculated using IPC2_protein)
Get csv file with sequences according to given criteria:
* You can choose from 21 different methods for calculating isoelectric point
Summary statistics related to proteome-wise predictions
Protein with the lowest isoelectric point:
>tr|A0A649VMC9|A0A649VMC9_9CAUD Uncharacterized protein OS=Arthrobacter phage Beagle OX=2656522 GN=94 PE=4 SV=1
MM1 pKa = 7.38 GLSISILVDD10 pKa = 3.38 VPDD13 pKa = 5.92 DD14 pKa = 3.71 IDD16 pKa = 3.78 PASVIRR22 pKa = 11.84 LGEE25 pKa = 4.09 RR26 pKa = 11.84 CCASDD31 pKa = 2.86 TGEE34 pKa = 3.88 PMGIGEE40 pKa = 5.36 AIQYY44 pKa = 9.92 AFNDD48 pKa = 4.09 PGGVTALATLGVTWTAIEE66 pKa = 4.12 TAA68 pKa = 3.91
Molecular weight: 6.97 kDa
Isoelectric point according different methods:
IPC2.protein.svr19 3.852
IPC2_protein 3.63
IPC_protein 3.49
Toseland 3.312
ProMoST 3.719
Dawson 3.516
Bjellqvist 3.694
Wikipedia 3.503
Rodwell 3.35
Grimsley 3.249
Solomon 3.452
Lehninger 3.414
Nozaki 3.681
DTASelect 3.834
Thurlkill 3.414
EMBOSS 3.516
Sillero 3.63
Patrickios 1.812
IPC_peptide 3.452
IPC2_peptide 3.592
IPC2.peptide.svr19 3.688
Protein with the highest isoelectric point:
>tr|A0A649VM56|A0A649VM56_9CAUD Portal protein OS=Arthrobacter phage Beagle OX=2656522 GN=23 PE=4 SV=1
MM1 pKa = 7.55 NNFTPEE7 pKa = 3.46 IRR9 pKa = 11.84 LCTGICGRR17 pKa = 11.84 MTRR20 pKa = 11.84 NSRR23 pKa = 11.84 HH24 pKa = 5.64 LKK26 pKa = 10.07 KK27 pKa = 10.6 DD28 pKa = 3.31 FPDD31 pKa = 3.53 TVSRR35 pKa = 11.84 QRR37 pKa = 11.84 DD38 pKa = 4.13 GKK40 pKa = 10.68 CRR42 pKa = 11.84 WCLHH46 pKa = 6.39 HH47 pKa = 6.38 EE48 pKa = 4.43 HH49 pKa = 6.73 EE50 pKa = 5.41 AGAAPAEE57 pKa = 4.49 RR58 pKa = 11.84 ATPMSGLQPCKK69 pKa = 10.21 GICGRR74 pKa = 11.84 LTRR77 pKa = 11.84 HH78 pKa = 5.56 CRR80 pKa = 11.84 VRR82 pKa = 11.84 LADD85 pKa = 3.68 APEE88 pKa = 4.19 SVTRR92 pKa = 11.84 VRR94 pKa = 11.84 DD95 pKa = 4.87 GKK97 pKa = 10.05 CQWCLDD103 pKa = 3.82 HH104 pKa = 7.84 GDD106 pKa = 4.19 EE107 pKa = 5.11 EE108 pKa = 4.65 NAQLPPRR115 pKa = 11.84 TGRR118 pKa = 11.84 PPRR121 pKa = 11.84 TTNRR125 pKa = 11.84 PVSEE129 pKa = 4.96 ANVQQAAAEE138 pKa = 4.19 YY139 pKa = 10.01 AAYY142 pKa = 8.49 TAARR146 pKa = 11.84 NARR149 pKa = 11.84 LARR152 pKa = 11.84 RR153 pKa = 11.84 EE154 pKa = 3.79 QRR156 pKa = 11.84 EE157 pKa = 3.73 RR158 pKa = 11.84 ASIQRR163 pKa = 11.84 QLLAARR169 pKa = 11.84 GQLLTAGSRR178 pKa = 11.84 RR179 pKa = 11.84 AA180 pKa = 3.35
Molecular weight: 20.2 kDa
Isoelectric point according different methods:
IPC2.protein.svr19 9.345
IPC2_protein 9.268
IPC_protein 9.999
Toseland 10.452
ProMoST 10.204
Dawson 10.54
Bjellqvist 10.335
Wikipedia 10.774
Rodwell 10.584
Grimsley 10.584
Solomon 10.701
Lehninger 10.672
Nozaki 10.555
DTASelect 10.292
Thurlkill 10.467
EMBOSS 10.862
Sillero 10.526
Patrickios 10.321
IPC_peptide 10.701
IPC2_peptide 9.911
IPC2.peptide.svr19 8.48
Peptides (in silico digests for buttom-up proteomics)
Below you can find
in silico digests of the whole proteome with Trypsin, Chymotrypsin, Trypsin+LysC, LysN, ArgC proteases suitable for different mass spec machines.
Try ESI
ChTry ESI
ArgC ESI
LysN ESI
TryLysC ESI
Try MALDI
ChTry MALDI
ArgC MALDI
LysN MALDI
TryLysC MALDI
Try LTQ
ChTry LTQ
ArgC LTQ
LysN LTQ
TryLysC LTQ
Try MSlow
ChTry MSlow
ArgC MSlow
LysN MSlow
TryLysC MSlow
Try MShigh
ChTry MShigh
ArgC MShigh
LysN MShigh
TryLysC MShigh
General Statistics
Number of major isoforms
Number of additional isoforms
Number of all proteins
Number of amino acids
Min. Seq. Length
Max. Seq. Length
Avg. Seq. Length
Avg. Mol. Weight
131
0
131
22500
25
1625
171.8
18.81
Amino acid frequency
Ala
Cys
Asp
Glu
Phe
Gly
His
Ile
Lys
Leu
11.276 ± 0.308
1.013 ± 0.147
6.244 ± 0.201
6.711 ± 0.298
3.142 ± 0.184
8.147 ± 0.247
2.356 ± 0.17
4.809 ± 0.204
4.049 ± 0.198
7.658 ± 0.211
Met
Asn
Gln
Pro
Arg
Ser
Thr
Val
Trp
Tyr
2.436 ± 0.102
3.311 ± 0.184
5.396 ± 0.262
3.191 ± 0.15
6.338 ± 0.281
5.569 ± 0.209
7.093 ± 0.301
6.769 ± 0.24
1.978 ± 0.099
2.516 ± 0.182
Note: For amino acid frequency statistics the error has been estimated with the bootstraping (x100) at the protein level
Most of the basic statistics you can see at this page can be downloaded from this CSV file
For dipeptide frequency statistics click here