Sulfolobales Mexican rudivirus 1
Average proteome isoelectric point is 6.32
Get precalculated fractions of proteins
Acidic
pI < 6.8
6.8-7.4
pI > 7.4
Basic
All
Note: above files contain also dissociation constants (pKa)
Virtual 2D-PAGE plot for 37 proteins (isoelectric point calculated using IPC2_protein)
Get csv file with sequences according to given criteria:
* You can choose from 21 different methods for calculating isoelectric point
Summary statistics related to proteome-wise predictions
Protein with the lowest isoelectric point:
>tr|K4NZG9|K4NZG9_9VIRU Uncharacterized protein OS=Sulfolobales Mexican rudivirus 1 OX=1245909 PE=4 SV=1
MM1 pKa = 6.91 VTLQQVSSVTVTSPWSDD18 pKa = 3.52 YY19 pKa = 11.71 NEE21 pKa = 4.24 MPNQIQYY28 pKa = 11.16 GSIVNPFYY36 pKa = 10.56 PVFIFSYY43 pKa = 11.08 VNTSNCGLWVVGYY56 pKa = 10.37 VSGDD60 pKa = 3.17 NAGVFFNHH68 pKa = 6.08 ACGWVNVSGMFYY80 pKa = 10.52 FDD82 pKa = 3.56 AFGYY86 pKa = 8.81 PYY88 pKa = 11.45 AMGGGYY94 pKa = 8.98 MSGVSSCANNGAVWWTFVNQKK115 pKa = 8.67 TGSVNAYY122 pKa = 7.3 CTGLIPNNDD131 pKa = 2.78 VSMDD135 pKa = 4.5 FISAVADD142 pKa = 3.9 LNQQNILLLSLDD154 pKa = 3.41 ISVMAFLIWIIPVSEE169 pKa = 4.35 ISTLLNNSSPPSIKK183 pKa = 10.09 FAVVKK188 pKa = 8.33 TTDD191 pKa = 2.97 VPNQYY196 pKa = 8.31 MQQFFPVIYY205 pKa = 10.02 DD206 pKa = 3.34 GNLIIGSTYY215 pKa = 10.85 DD216 pKa = 3.36 YY217 pKa = 11.03 VAYY220 pKa = 10.07 LAVMPLSEE228 pKa = 4.3 IYY230 pKa = 10.32 VNAMTGIPTTNTPATYY246 pKa = 10.64 VGTQYY251 pKa = 10.77 TYY253 pKa = 10.99 SEE255 pKa = 4.82 YY256 pKa = 11.02 EE257 pKa = 4.01 FVTWPIITTIAIPSSGGLSIYY278 pKa = 10.22 VSLMLGAKK286 pKa = 10.02 NYY288 pKa = 10.64 LLYY291 pKa = 10.24 IANLSPSNWSVVSSFTFYY309 pKa = 10.41 LTDD312 pKa = 3.37 VASSICTAPVGSTWNGVFILMFNGPSSSCNTLYY345 pKa = 11.06 VVVADD350 pKa = 4.67 PKK352 pKa = 10.13 SSNYY356 pKa = 9.88 EE357 pKa = 3.5 YY358 pKa = 10.34 TYY360 pKa = 11.3 INMTQNTDD368 pKa = 2.96 IAILGYY374 pKa = 10.09 EE375 pKa = 4.62 GYY377 pKa = 10.55 LVVSQQPLSDD387 pKa = 3.58 STVTFVVYY395 pKa = 10.29 QILLDD400 pKa = 3.57 HH401 pKa = 6.78 TYY403 pKa = 10.38 VFQNVAVSTTSSTITFSGVLYY424 pKa = 10.54 DD425 pKa = 4.06 EE426 pKa = 4.66 TAGAPVANATVWLVAVRR443 pKa = 11.84 SYY445 pKa = 11.14 VKK447 pKa = 10.29 QYY449 pKa = 11.38 SNDD452 pKa = 3.56 VVPLASTTTDD462 pKa = 3.03 SAGHH466 pKa = 5.15 FTVSAPMQSGYY477 pKa = 10.25 NAYY480 pKa = 9.96 GVLYY484 pKa = 9.78 TPP486 pKa = 5.2
Molecular weight: 52.99 kDa
Isoelectric point according different methods:
IPC2.protein.svr19 3.899
IPC2_protein 3.821
IPC_protein 3.821
Toseland 3.592
ProMoST 3.999
Dawson 3.821
Bjellqvist 3.973
Wikipedia 3.77
Rodwell 3.643
Grimsley 3.503
Solomon 3.808
Lehninger 3.77
Nozaki 3.935
DTASelect 4.202
Thurlkill 3.656
EMBOSS 3.783
Sillero 3.948
Patrickios 0.006
IPC_peptide 3.808
IPC2_peptide 3.91
IPC2.peptide.svr19 3.83
Protein with the highest isoelectric point:
>tr|K4NX87|K4NX87_9VIRU Putative SAM-dependent methyltransferase OS=Sulfolobales Mexican rudivirus 1 OX=1245909 PE=4 SV=1
MM1 pKa = 7.55 HH2 pKa = 7.32 NGAQKK7 pKa = 10.48 RR8 pKa = 11.84 VIKK11 pKa = 9.94 LINVIRR17 pKa = 11.84 RR18 pKa = 11.84 RR19 pKa = 11.84 YY20 pKa = 9.09 KK21 pKa = 9.85 PPAVRR26 pKa = 11.84 TWIFHH31 pKa = 5.57 YY32 pKa = 9.58 IWHH35 pKa = 6.15 GRR37 pKa = 11.84 SYY39 pKa = 10.59 TIATIAVRR47 pKa = 11.84 LTGDD51 pKa = 3.32 GALHH55 pKa = 5.25 VRR57 pKa = 11.84 KK58 pKa = 10.14 KK59 pKa = 10.4 IEE61 pKa = 3.9 ARR63 pKa = 11.84 LKK65 pKa = 10.32 ALEE68 pKa = 4.35 GVNVTTFLDD77 pKa = 3.75 GEE79 pKa = 4.57 YY80 pKa = 10.2 IKK82 pKa = 10.47 YY83 pKa = 8.51 TLEE86 pKa = 3.86 AA87 pKa = 4.73
Molecular weight: 10.1 kDa
Isoelectric point according different methods:
IPC2.protein.svr19 9.297
IPC2_protein 9.94
IPC_protein 10.584
Toseland 10.599
ProMoST 10.277
Dawson 10.745
Bjellqvist 10.438
Wikipedia 10.935
Rodwell 11.082
Grimsley 10.804
Solomon 10.804
Lehninger 10.774
Nozaki 10.57
DTASelect 10.438
Thurlkill 10.613
EMBOSS 10.994
Sillero 10.657
Patrickios 10.818
IPC_peptide 10.818
IPC2_peptide 9.194
IPC2.peptide.svr19 8.501
Peptides (in silico digests for buttom-up proteomics)
Below you can find
in silico digests of the whole proteome with Trypsin, Chymotrypsin, Trypsin+LysC, LysN, ArgC proteases suitable for different mass spec machines.
Try ESI
ChTry ESI
ArgC ESI
LysN ESI
TryLysC ESI
Try MALDI
ChTry MALDI
ArgC MALDI
LysN MALDI
TryLysC MALDI
Try LTQ
ChTry LTQ
ArgC LTQ
LysN LTQ
TryLysC LTQ
Try MSlow
ChTry MSlow
ArgC MSlow
LysN MSlow
TryLysC MSlow
Try MShigh
ChTry MShigh
ArgC MShigh
LysN MShigh
TryLysC MShigh
General Statistics
Number of major isoforms
Number of additional isoforms
Number of all proteins
Number of amino acids
Min. Seq. Length
Max. Seq. Length
Avg. Seq. Length
Avg. Mol. Weight
37
0
37
7852
54
1106
212.2
24.08
Amino acid frequency
Ala
Cys
Asp
Glu
Phe
Gly
His
Ile
Lys
Leu
7.221 ± 0.415
0.777 ± 0.177
4.648 ± 0.366
6.011 ± 0.711
4.661 ± 0.335
5.018 ± 0.347
1.617 ± 0.23
6.572 ± 0.36
5.183 ± 0.477
10.15 ± 0.452
Met
Asn
Gln
Pro
Arg
Ser
Thr
Val
Trp
Tyr
2.394 ± 0.177
5.323 ± 0.364
4.075 ± 0.346
4.483 ± 0.416
4.457 ± 0.498
6.597 ± 0.626
5.693 ± 0.347
8.482 ± 0.479
1.032 ± 0.186
5.604 ± 0.34
Note: For amino acid frequency statistics the error has been estimated with the bootstraping (x100) at the protein level
Most of the basic statistics you can see at this page can be downloaded from this CSV file
For dipeptide frequency statistics click here