Parabacteroides sp. CAG:409

Taxonomy: cellular organisms; Bacteria; FCB group; Bacteroidetes/Chlorobi group; Bacteroidetes; Bacteroidia; Bacteroidales; Tannerellaceae; Parabacteroides; environmental samples

Average proteome isoelectric point is 6.24

Get precalculated fractions of proteins

Acidic
pI < 6.8
6.8-7.4
pI > 7.4
Basic
    
All



Virtual 2D-PAGE plot for 2823 proteins (isoelectric point calculated using IPC2_protein)

Get csv file with sequences according to given criteria:
        -     Method 

     -  kDa    
                                                                                      

* You can choose from 21 different methods for calculating isoelectric point

Summary statistics related to proteome-wise predictions


    
Protein with the lowest isoelectric point:
>tr|R7JMQ7|R7JMQ7_9BACT TPR_REGION domain-containing protein OS=Parabacteroides sp. CAG:409 OX=1262913 GN=BN646_02786 PE=4 SV=1
MM1 pKa = 7.04KK2 pKa = 10.05TSNLKK7 pKa = 9.71FLSMAAISAMIAFTSCDD24 pKa = 3.56EE25 pKa = 5.68DD26 pKa = 5.85DD27 pKa = 4.31PTDD30 pKa = 3.68NPNTDD35 pKa = 2.96GTEE38 pKa = 4.13NPDD41 pKa = 3.58GEE43 pKa = 4.49ATGYY47 pKa = 10.47SSEE50 pKa = 4.34LFQAEE55 pKa = 4.39ANHH58 pKa = 5.44NTINFDD64 pKa = 3.89FSNTFSNLFVGTIDD78 pKa = 4.6GGADD82 pKa = 3.29MPSVNSFFTAAAYY95 pKa = 9.83QGAVSADD102 pKa = 3.84NNWTAGWTLTTEE114 pKa = 3.94EE115 pKa = 4.8GGDD118 pKa = 3.33NKK120 pKa = 10.79ADD122 pKa = 3.63QEE124 pKa = 4.32AVEE127 pKa = 4.5TLTGTLSEE135 pKa = 4.86DD136 pKa = 3.28KK137 pKa = 10.63TLEE140 pKa = 4.06ANKK143 pKa = 10.59KK144 pKa = 9.34YY145 pKa = 9.58MLSGDD150 pKa = 4.17YY151 pKa = 10.2IVEE154 pKa = 4.23TGATLTIPEE163 pKa = 4.49GVTIVAEE170 pKa = 4.21ADD172 pKa = 3.59DD173 pKa = 5.37DD174 pKa = 4.07KK175 pKa = 11.82VDD177 pKa = 4.41YY178 pKa = 11.0ILIQQGAKK186 pKa = 9.55IVAEE190 pKa = 4.49GTAEE194 pKa = 3.95NPIVMTATKK203 pKa = 10.15KK204 pKa = 10.31EE205 pKa = 3.68AGAWGGIHH213 pKa = 6.73ICGKK217 pKa = 9.29AHH219 pKa = 6.51SNVEE223 pKa = 3.9GGTGKK228 pKa = 10.21SEE230 pKa = 4.39IGDD233 pKa = 3.51ATYY236 pKa = 10.97GGNDD240 pKa = 3.52DD241 pKa = 4.6ADD243 pKa = 3.46NSGVLKK249 pKa = 9.86YY250 pKa = 10.89LRR252 pKa = 11.84IEE254 pKa = 3.93YY255 pKa = 10.08SGYY258 pKa = 11.01AFDD261 pKa = 6.0SEE263 pKa = 4.99HH264 pKa = 6.46EE265 pKa = 4.31ANGFTFYY272 pKa = 11.24GVGNGTTIEE281 pKa = 4.01YY282 pKa = 9.15CQAYY286 pKa = 9.13KK287 pKa = 10.93GSDD290 pKa = 3.2DD291 pKa = 3.48GFEE294 pKa = 4.15FFGGSVNVKK303 pKa = 9.97NVIAVSCSDD312 pKa = 5.48DD313 pKa = 3.8SFDD316 pKa = 3.8WTEE319 pKa = 3.82GWKK322 pKa = 11.09GNGQFMIAYY331 pKa = 9.2QEE333 pKa = 4.32AEE335 pKa = 3.93EE336 pKa = 4.28SLGYY340 pKa = 10.84DD341 pKa = 3.91CDD343 pKa = 5.24CLMEE347 pKa = 5.18CDD349 pKa = 3.92NNGDD353 pKa = 3.66NFAATPVAHH362 pKa = 6.59PTLANLTLIGNGGSKK377 pKa = 10.06QGIRR381 pKa = 11.84LRR383 pKa = 11.84AGTQASIYY391 pKa = 9.12NALVAGKK398 pKa = 9.68GMCLTTEE405 pKa = 3.95TAEE408 pKa = 4.26TEE410 pKa = 4.13DD411 pKa = 3.92ALVEE415 pKa = 4.31GTSVLNYY422 pKa = 8.22ITLATDD428 pKa = 3.9ITCKK432 pKa = 10.34GAEE435 pKa = 3.89

Molecular weight: 45.98 kDa
Isoelectric point according different methods:





Protein with the highest isoelectric point:
>tr|R7JCG2|R7JCG2_9BACT DUF5672 domain-containing protein OS=Parabacteroides sp. CAG:409 OX=1262913 GN=BN646_01290 PE=4 SV=1
MM1 pKa = 7.25TSAIFSPAATCCPGVTFTKK20 pKa = 10.34LSCPSIGEE28 pKa = 4.18RR29 pKa = 11.84TTKK32 pKa = 10.65SSIRR36 pKa = 11.84CRR38 pKa = 11.84LRR40 pKa = 11.84INFFSSRR47 pKa = 11.84SRR49 pKa = 11.84SFCNNSFRR57 pKa = 11.84ITVMEE62 pKa = 4.03EE63 pKa = 3.91SLWRR67 pKa = 11.84LFNFRR72 pKa = 11.84RR73 pKa = 11.84VDD75 pKa = 3.6SVLYY79 pKa = 9.93RR80 pKa = 11.84YY81 pKa = 10.17SSSDD85 pKa = 3.17TSSWALLRR93 pKa = 11.84IPIAYY98 pKa = 10.2SSFSSEE104 pKa = 3.61KK105 pKa = 10.49LFRR108 pKa = 11.84ILFNSYY114 pKa = 9.85SASNVCCFKK123 pKa = 10.96VRR125 pKa = 11.84LFCSISRR132 pKa = 11.84AFSS135 pKa = 3.18

Molecular weight: 15.47 kDa
Isoelectric point according different methods:





Peptides (in silico digests for buttom-up proteomics)

Below you can find in silico digests of the whole proteome with Trypsin, Chymotrypsin, Trypsin+LysC, LysN, ArgC proteases suitable for different mass spec machines.

Try
ESI
ChTry
ESI
ArgC
ESI
LysN
ESI
TryLysC
ESI

Try
MALDI
ChTry
MALDI
ArgC
MALDI
LysN
MALDI
TryLysC
MALDI

Try
LTQ
ChTry
LTQ
ArgC
LTQ
LysN
LTQ
TryLysC
LTQ

Try
MSlow
ChTry
MSlow
ArgC
MSlow
LysN
MSlow
TryLysC
MSlow

Try
MShigh
ChTry
MShigh
ArgC
MShigh
LysN
MShigh
TryLysC
MShigh

General Statistics

Number of major isoforms

Number of additional isoforms

Number of all proteins

Number of amino acids

Min. Seq. Length

Max. Seq. Length

Avg. Seq. Length

Avg. Mol. Weight

2823

0

2823

967119

30

2471

342.6

38.69

Amino acid frequency

Ala

Cys

Asp

Glu

Phe

Gly

His

Ile

Lys

Leu

7.095 ± 0.041

1.238 ± 0.016

5.483 ± 0.029

6.71 ± 0.045

4.585 ± 0.034

6.773 ± 0.045

1.955 ± 0.02

6.843 ± 0.044

6.421 ± 0.046

9.307 ± 0.051

Met

Asn

Gln

Pro

Arg

Ser

Thr

Val

Trp

Tyr

2.72 ± 0.021

4.783 ± 0.032

3.907 ± 0.028

3.963 ± 0.029

4.504 ± 0.03

6.035 ± 0.035

5.383 ± 0.031

6.509 ± 0.034

1.337 ± 0.018

4.429 ± 0.028
Note: For amino acid frequency statistics the error has been estimated with the bootstraping (x100) at the protein level

Most of the basic statistics you can see at this page can be downloaded from this CSV file

For dipeptide frequency statistics click here
See this proteome in: uniprot_link
Proteome-pI is available under Creative Commons Attribution-NoDerivs license, for more details see here

Reference: Kozlowski LP. Proteome-pI 2.0: Proteome Isoelectric Point Database Update. Nucleic Acids Res. 2021, doi: 10.1093/nar/gkab944 Contact: Lukasz P. Kozlowski