Caryophanon tenue
Average proteome isoelectric point is 5.96
Get precalculated fractions of proteins
Acidic
pI < 6.8
6.8-7.4
pI > 7.4
Basic
All
Note: above files contain also dissociation constants (pKa)
Virtual 2D-PAGE plot for 3090 proteins (isoelectric point calculated using IPC2_protein)
Get csv file with sequences according to given criteria:
* You can choose from 21 different methods for calculating isoelectric point
Summary statistics related to proteome-wise predictions
Protein with the lowest isoelectric point:
>tr|A0A1C0YBX0|A0A1C0YBX0_9BACL GCN5 family acetyltransferase OS=Caryophanon tenue OX=33978 GN=A6M13_03605 PE=4 SV=1
MM1 pKa = 7.7 RR2 pKa = 11.84 KK3 pKa = 8.45 YY4 pKa = 10.89 AVMVGAVLMLVACNEE19 pKa = 4.06 QVNEE23 pKa = 4.11 EE24 pKa = 4.5 TIKK27 pKa = 9.36 PTPVTDD33 pKa = 3.92 TAATEE38 pKa = 4.12 PVGGEE43 pKa = 3.8 TSEE46 pKa = 4.12 TRR48 pKa = 11.84 EE49 pKa = 3.91 SEE51 pKa = 4.13 GRR53 pKa = 11.84 DD54 pKa = 3.48 DD55 pKa = 4.11 EE56 pKa = 4.49 QVVIPEE62 pKa = 4.07 PQMDD66 pKa = 3.93 EE67 pKa = 4.15 EE68 pKa = 4.42 DD69 pKa = 4.1 VEE71 pKa = 4.76 VSDD74 pKa = 5.84 EE75 pKa = 3.96 VDD77 pKa = 3.34 EE78 pKa = 4.65 PQEE81 pKa = 4.23 SVSQEE86 pKa = 3.64 NVTEE90 pKa = 4.75 DD91 pKa = 3.9 EE92 pKa = 4.27 LLMWIYY98 pKa = 11.15 KK99 pKa = 9.22 MEE101 pKa = 4.49 DD102 pKa = 2.58 WSGYY106 pKa = 9.87 SSLLRR111 pKa = 11.84 IEE113 pKa = 4.92 NVYY116 pKa = 11.17 DD117 pKa = 3.96 DD118 pKa = 4.34 EE119 pKa = 4.53 QLNTMTQQEE128 pKa = 4.48 TTYY131 pKa = 10.95 VWMPEE136 pKa = 3.6 QLYY139 pKa = 8.28 TVTQHH144 pKa = 6.75 AGISTTLIEE153 pKa = 4.14 QYY155 pKa = 7.91 MTEE158 pKa = 3.97 EE159 pKa = 3.64 EE160 pKa = 4.29 AYY162 pKa = 10.1 EE163 pKa = 4.13 RR164 pKa = 11.84 LNLDD168 pKa = 2.54 EE169 pKa = 4.47 WLPLEE174 pKa = 4.31 GEE176 pKa = 4.3 RR177 pKa = 11.84 AYY179 pKa = 11.27 SPLPAKK185 pKa = 10.13 MSFFEE190 pKa = 5.42 LMLEE194 pKa = 4.19 SSAYY198 pKa = 9.05 TVEE201 pKa = 4.2 QQNEE205 pKa = 4.02 AAHH208 pKa = 5.29 VTFTVQPDD216 pKa = 3.94 NIAYY220 pKa = 9.46 VYY222 pKa = 11.55 AMLQRR227 pKa = 11.84 AFNIQMDD234 pKa = 4.16 EE235 pKa = 4.27 TVTAEE240 pKa = 4.12 DD241 pKa = 3.96 IYY243 pKa = 11.63 AEE245 pKa = 3.74 FHH247 pKa = 6.15 YY248 pKa = 10.54 EE249 pKa = 4.89 DD250 pKa = 5.72 RR251 pKa = 11.84 FLQQYY256 pKa = 10.01 KK257 pKa = 8.67 ITVDD261 pKa = 4.18 FDD263 pKa = 3.51 AAEE266 pKa = 4.38 DD267 pKa = 3.93 DD268 pKa = 3.93 MPKK271 pKa = 10.66 EE272 pKa = 3.89 LVIDD276 pKa = 3.89 EE277 pKa = 5.42 AFTDD281 pKa = 3.94 MNQVEE286 pKa = 4.84 ALPARR291 pKa = 11.84 DD292 pKa = 3.5 TLL294 pKa = 4.03
Molecular weight: 33.93 kDa
Isoelectric point according different methods:
IPC2.protein.svr19 3.763
IPC2_protein 3.783
IPC_protein 3.757
Toseland 3.579
ProMoST 3.884
Dawson 3.719
Bjellqvist 3.872
Wikipedia 3.592
Rodwell 3.592
Grimsley 3.49
Solomon 3.706
Lehninger 3.668
Nozaki 3.821
DTASelect 3.973
Thurlkill 3.592
EMBOSS 3.605
Sillero 3.872
Patrickios 1.825
IPC_peptide 3.719
IPC2_peptide 3.859
IPC2.peptide.svr19 3.784
Protein with the highest isoelectric point:
>tr|A0A1C0YLC6|A0A1C0YLC6_9BACL Fumarate hydratase class II OS=Caryophanon tenue OX=33978 GN=fumC PE=3 SV=1
MM1 pKa = 7.36 KK2 pKa = 9.43 RR3 pKa = 11.84 TYY5 pKa = 9.54 QPKK8 pKa = 9.44 KK9 pKa = 7.96 RR10 pKa = 11.84 KK11 pKa = 8.69 HH12 pKa = 5.94 SKK14 pKa = 8.54 VHH16 pKa = 5.66 GFRR19 pKa = 11.84 ARR21 pKa = 11.84 MATKK25 pKa = 10.31 NGRR28 pKa = 11.84 NVLARR33 pKa = 11.84 RR34 pKa = 11.84 RR35 pKa = 11.84 KK36 pKa = 9.36 KK37 pKa = 10.05 GRR39 pKa = 11.84 KK40 pKa = 8.66 VLSAA44 pKa = 4.05
Molecular weight: 5.23 kDa
Isoelectric point according different methods:
IPC2.protein.svr19 9.393
IPC2_protein 11.067
IPC_protein 12.398
Toseland 12.559
ProMoST 13.042
Dawson 12.574
Bjellqvist 12.559
Wikipedia 13.027
Rodwell 12.398
Grimsley 12.603
Solomon 13.056
Lehninger 12.954
Nozaki 12.559
DTASelect 12.559
Thurlkill 12.559
EMBOSS 13.056
Sillero 12.559
Patrickios 12.135
IPC_peptide 13.056
IPC2_peptide 12.032
IPC2.peptide.svr19 8.988
Peptides (in silico digests for buttom-up proteomics)
Below you can find
in silico digests of the whole proteome with Trypsin, Chymotrypsin, Trypsin+LysC, LysN, ArgC proteases suitable for different mass spec machines.
Try ESI
ChTry ESI
ArgC ESI
LysN ESI
TryLysC ESI
Try MALDI
ChTry MALDI
ArgC MALDI
LysN MALDI
TryLysC MALDI
Try LTQ
ChTry LTQ
ArgC LTQ
LysN LTQ
TryLysC LTQ
Try MSlow
ChTry MSlow
ArgC MSlow
LysN MSlow
TryLysC MSlow
Try MShigh
ChTry MShigh
ArgC MShigh
LysN MShigh
TryLysC MShigh
General Statistics
Number of major isoforms
Number of additional isoforms
Number of all proteins
Number of amino acids
Min. Seq. Length
Max. Seq. Length
Avg. Seq. Length
Avg. Mol. Weight
3090
0
3090
949518
37
2089
307.3
34.35
Amino acid frequency
Ala
Cys
Asp
Glu
Phe
Gly
His
Ile
Lys
Leu
8.859 ± 0.044
0.693 ± 0.011
5.159 ± 0.034
7.089 ± 0.048
4.391 ± 0.037
6.352 ± 0.041
2.372 ± 0.027
7.237 ± 0.04
5.291 ± 0.038
9.478 ± 0.046
Met
Asn
Gln
Pro
Arg
Ser
Thr
Val
Trp
Tyr
2.881 ± 0.025
3.785 ± 0.031
3.506 ± 0.026
4.833 ± 0.044
4.189 ± 0.03
5.123 ± 0.034
6.58 ± 0.043
7.577 ± 0.04
0.901 ± 0.014
3.702 ± 0.031
Note: For amino acid frequency statistics the error has been estimated with the bootstraping (x100) at the protein level
Most of the basic statistics you can see at this page can be downloaded from this CSV file
For dipeptide frequency statistics click here