Mosqueiro virus
Average proteome isoelectric point is 6.8
Get precalculated fractions of proteins
Acidic
pI < 6.8
6.8-7.4
pI > 7.4
Basic
All
Note: above files contain also dissociation constants (pKa)
Virtual 2D-PAGE plot for 11 proteins (isoelectric point calculated using IPC2_protein)
Get csv file with sequences according to given criteria:
* You can choose from 21 different methods for calculating isoelectric point
Summary statistics related to proteome-wise predictions
Protein with the lowest isoelectric point:
>tr|A0A0D3R1X4|A0A0D3R1X4_9RHAB Nucleocapsid protein OS=Mosqueiro virus OX=200403 PE=4 SV=1
MM1 pKa = 7.59 EE2 pKa = 5.16 FFVSLGLSFSSTKK15 pKa = 10.69 KK16 pKa = 10.69 NITLEE21 pKa = 5.24 DD22 pKa = 3.67 IDD24 pKa = 5.88 LMIKK28 pKa = 10.3 KK29 pKa = 8.89 MVVRR33 pKa = 11.84 THH35 pKa = 6.37 QIHH38 pKa = 6.72 GLPVDD43 pKa = 4.74 LGGLLINLLWEE54 pKa = 4.37 HH55 pKa = 6.23 TDD57 pKa = 3.61 FRR59 pKa = 11.84 TDD61 pKa = 3.05 CRR63 pKa = 11.84 GNLMGYY69 pKa = 10.3 GYY71 pKa = 11.01 LEE73 pKa = 4.94 ASLSTPGTLVQVDD86 pKa = 4.24 LMDD89 pKa = 3.53 KK90 pKa = 8.41 TFCIHH95 pKa = 7.41 DD96 pKa = 3.72 GWEE99 pKa = 4.21 HH100 pKa = 7.33 DD101 pKa = 4.38 DD102 pKa = 5.45 LGIIDD107 pKa = 3.72 QVDD110 pKa = 3.8 YY111 pKa = 10.54 KK112 pKa = 11.14 VCVYY116 pKa = 8.71 PSPMEE121 pKa = 4.34 DD122 pKa = 3.1 PVPIMEE128 pKa = 3.99 YY129 pKa = 9.56 WIPVDD134 pKa = 3.5 VSYY137 pKa = 11.76 GNYY140 pKa = 9.77 LRR142 pKa = 11.84 RR143 pKa = 11.84 NPDD146 pKa = 2.4 IVANLYY152 pKa = 9.97 GFKK155 pKa = 10.53 HH156 pKa = 6.62 LISLCYY162 pKa = 10.06
Molecular weight: 18.57 kDa
Isoelectric point according different methods:
IPC2.protein.svr19 4.48
IPC2_protein 4.736
IPC_protein 4.673
Toseland 4.533
ProMoST 4.838
Dawson 4.673
Bjellqvist 4.813
Wikipedia 4.596
Rodwell 4.546
Grimsley 4.457
Solomon 4.673
Lehninger 4.635
Nozaki 4.8
DTASelect 5.029
Thurlkill 4.571
EMBOSS 4.622
Sillero 4.838
Patrickios 2.053
IPC_peptide 4.673
IPC2_peptide 4.825
IPC2.peptide.svr19 4.73
Protein with the highest isoelectric point:
>tr|A0A0D3R1I8|A0A0D3R1I8_9RHAB Phosphoprotein OS=Mosqueiro virus OX=200403 PE=4 SV=1
MM1 pKa = 7.36 MIWKK5 pKa = 7.56 MTLLCFPKK13 pKa = 9.92 WNLNIVAIQIGLMQCLTPRR32 pKa = 11.84 SSLHH36 pKa = 5.1 RR37 pKa = 11.84 WKK39 pKa = 10.21 KK40 pKa = 9.43 RR41 pKa = 11.84 KK42 pKa = 9.82 GMTIFIRR49 pKa = 11.84 LEE51 pKa = 3.84 RR52 pKa = 11.84 LDD54 pKa = 4.81 CLTISQLLRR63 pKa = 11.84 NLTLSMILSASFHH76 pKa = 6.24 KK77 pKa = 10.86
Molecular weight: 9.13 kDa
Isoelectric point according different methods:
IPC2.protein.svr19 9.274
IPC2_protein 9.75
IPC_protein 10.482
Toseland 11.228
ProMoST 11.199
Dawson 11.257
Bjellqvist 11.008
Wikipedia 11.506
Rodwell 11.506
Grimsley 11.272
Solomon 11.506
Lehninger 11.462
Nozaki 11.213
DTASelect 11.008
Thurlkill 11.199
EMBOSS 11.652
Sillero 11.213
Patrickios 11.272
IPC_peptide 11.52
IPC2_peptide 10.335
IPC2.peptide.svr19 9.021
Peptides (in silico digests for buttom-up proteomics)
Below you can find
in silico digests of the whole proteome with Trypsin, Chymotrypsin, Trypsin+LysC, LysN, ArgC proteases suitable for different mass spec machines.
Try ESI
ChTry ESI
ArgC ESI
LysN ESI
TryLysC ESI
Try MALDI
ChTry MALDI
ArgC MALDI
LysN MALDI
TryLysC MALDI
Try LTQ
ChTry LTQ
ArgC LTQ
LysN LTQ
TryLysC LTQ
Try MSlow
ChTry MSlow
ArgC MSlow
LysN MSlow
TryLysC MSlow
Try MShigh
ChTry MShigh
ArgC MShigh
LysN MShigh
TryLysC MShigh
General Statistics
Number of major isoforms
Number of additional isoforms
Number of all proteins
Number of amino acids
Min. Seq. Length
Max. Seq. Length
Avg. Seq. Length
Avg. Mol. Weight
11
0
11
4329
72
2105
393.5
45.15
Amino acid frequency
Ala
Cys
Asp
Glu
Phe
Gly
His
Ile
Lys
Leu
4.481 ± 0.489
1.617 ± 0.237
5.752 ± 0.512
5.267 ± 0.427
4.689 ± 0.267
5.821 ± 0.327
2.449 ± 0.213
7.669 ± 0.451
6.191 ± 0.622
10.095 ± 0.866
Met
Asn
Gln
Pro
Arg
Ser
Thr
Val
Trp
Tyr
2.795 ± 0.491
5.683 ± 0.463
4.412 ± 0.292
3.581 ± 0.307
4.897 ± 0.32
7.461 ± 0.295
5.221 ± 0.287
5.59 ± 0.243
2.033 ± 0.29
4.297 ± 0.296
Note: For amino acid frequency statistics the error has been estimated with the bootstraping (x100) at the protein level
Most of the basic statistics you can see at this page can be downloaded from this CSV file
For dipeptide frequency statistics click here