Amino acid dipepetide frequency for Bacillus sp. MUM 13

Apart from single amino acid frequencies one can also calculate so called amino acid dipeptide frequency.

There is 400 possibilites (441 if we consider Xaa as additional 21st amino acid representing all non-standard or unknown amino acids). Thus, if the dipeptides would be present randomly in proteins each amino acid dipeptide should be present with 0.25% frequency. As it is not the case in the nature, for better visablity all more than expected dipepetides are marked by red, and those which are underrepresented are marked by blue in the table.

All values are presented as per milles (‰), therefore need to be multiplied by 10-3.

For more information see sequence space article on Wikipedia.

AlaCysAspGluPheGlyHisIleLysLeuMetAsnProGlnArgSerThrValTrpTyrXaa
Ala
7.54AlaAla: 7.54 ± 0.117
0.628AlaCys: 0.628 ± 0.024
3.784AlaAsp: 3.784 ± 0.062
5.213AlaGlu: 5.213 ± 0.072
3.293AlaPhe: 3.293 ± 0.058
6.364AlaGly: 6.364 ± 0.082
1.327AlaHis: 1.327 ± 0.035
5.835AlaIle: 5.835 ± 0.087
4.886AlaLys: 4.886 ± 0.063
7.143AlaLeu: 7.143 ± 0.099
1.998AlaMet: 1.998 ± 0.045
2.575AlaAsn: 2.575 ± 0.045
2.157AlaPro: 2.157 ± 0.047
2.156AlaGln: 2.156 ± 0.046
2.749AlaArg: 2.749 ± 0.051
4.633AlaSer: 4.633 ± 0.059
2.589AlaThr: 2.589 ± 0.048
5.632AlaVal: 5.632 ± 0.07
0.626AlaTrp: 0.626 ± 0.022
2.288AlaTyr: 2.288 ± 0.037
0.0AlaXaa: 0.0 ± 0.0
Cys
0.484CysAla: 0.484 ± 0.022
0.101CysCys: 0.101 ± 0.009
0.382CysAsp: 0.382 ± 0.018
0.467CysGlu: 0.467 ± 0.02
0.335CysPhe: 0.335 ± 0.019
0.698CysGly: 0.698 ± 0.025
0.198CysHis: 0.198 ± 0.014
0.58CysIle: 0.58 ± 0.023
0.38CysLys: 0.38 ± 0.018
0.76CysLeu: 0.76 ± 0.025
0.202CysMet: 0.202 ± 0.013
0.268CysAsn: 0.268 ± 0.015
0.362CysPro: 0.362 ± 0.016
0.223CysGln: 0.223 ± 0.015
0.385CysArg: 0.385 ± 0.017
0.588CysSer: 0.588 ± 0.021
0.403CysThr: 0.403 ± 0.019
0.403CysVal: 0.403 ± 0.019
0.074CysTrp: 0.074 ± 0.008
0.278CysTyr: 0.278 ± 0.016
0.0CysXaa: 0.0 ± 0.0
Asp
3.343AspAla: 3.343 ± 0.058
0.411AspCys: 0.411 ± 0.018
2.298AspAsp: 2.298 ± 0.046
4.023AspGlu: 4.023 ± 0.071
2.565AspPhe: 2.565 ± 0.046
3.343AspGly: 3.343 ± 0.057
1.099AspHis: 1.099 ± 0.03
4.354AspIle: 4.354 ± 0.071
3.369AspLys: 3.369 ± 0.053
4.89AspLeu: 4.89 ± 0.07
1.38AspMet: 1.38 ± 0.036
1.732AspAsn: 1.732 ± 0.045
1.922AspPro: 1.922 ± 0.044
1.666AspGln: 1.666 ± 0.04
2.105AspArg: 2.105 ± 0.044
3.077AspSer: 3.077 ± 0.057
2.373AspThr: 2.373 ± 0.043
3.32AspVal: 3.32 ± 0.051
0.621AspTrp: 0.621 ± 0.023
2.063AspTyr: 2.063 ± 0.048
0.0AspXaa: 0.0 ± 0.0
Glu
5.353GluAla: 5.353 ± 0.091
0.425GluCys: 0.425 ± 0.02
3.64GluAsp: 3.64 ± 0.065
6.481GluGlu: 6.481 ± 0.103
2.637GluPhe: 2.637 ± 0.048
4.372GluGly: 4.372 ± 0.07
1.459GluHis: 1.459 ± 0.037
5.855GluIle: 5.855 ± 0.082
6.707GluLys: 6.707 ± 0.078
6.901GluLeu: 6.901 ± 0.078
2.232GluMet: 2.232 ± 0.043
3.82GluAsn: 3.82 ± 0.061
1.758GluPro: 1.758 ± 0.039
2.869GluGln: 2.869 ± 0.055
3.362GluArg: 3.362 ± 0.058
3.648GluSer: 3.648 ± 0.053
3.644GluThr: 3.644 ± 0.057
4.303GluVal: 4.303 ± 0.067
0.739GluTrp: 0.739 ± 0.027
2.283GluTyr: 2.283 ± 0.048
0.0GluXaa: 0.0 ± 0.0
Phe
3.065PheAla: 3.065 ± 0.051
0.4PheCys: 0.4 ± 0.019
2.231PheAsp: 2.231 ± 0.041
2.717PheGlu: 2.717 ± 0.05
2.397PhePhe: 2.397 ± 0.056
3.256PheGly: 3.256 ± 0.053
1.029PheHis: 1.029 ± 0.037
3.943PheIle: 3.943 ± 0.068
2.603PheLys: 2.603 ± 0.049
4.666PheLeu: 4.666 ± 0.074
1.251PheMet: 1.251 ± 0.033
1.893PheAsn: 1.893 ± 0.037
1.796PhePro: 1.796 ± 0.042
1.612PheGln: 1.612 ± 0.037
1.704PheArg: 1.704 ± 0.038
3.61PheSer: 3.61 ± 0.059
2.604PheThr: 2.604 ± 0.048
2.726PheVal: 2.726 ± 0.054
0.47PheTrp: 0.47 ± 0.025
1.767PheTyr: 1.767 ± 0.042
0.0PheXaa: 0.0 ± 0.0
Gly
4.887GlyAla: 4.887 ± 0.074
0.668GlyCys: 0.668 ± 0.022
3.267GlyAsp: 3.267 ± 0.05
4.598GlyGlu: 4.598 ± 0.063
3.468GlyPhe: 3.468 ± 0.059
4.946GlyGly: 4.946 ± 0.096
1.458GlyHis: 1.458 ± 0.04
6.376GlyIle: 6.376 ± 0.083
5.685GlyLys: 5.685 ± 0.064
6.709GlyLeu: 6.709 ± 0.088
2.215GlyMet: 2.215 ± 0.047
2.941GlyAsn: 2.941 ± 0.055
1.774GlyPro: 1.774 ± 0.038
2.27GlyGln: 2.27 ± 0.042
2.852GlyArg: 2.852 ± 0.051
4.439GlySer: 4.439 ± 0.066
4.06GlyThr: 4.06 ± 0.058
4.58GlyVal: 4.58 ± 0.066
0.78GlyTrp: 0.78 ± 0.023
2.87GlyTyr: 2.87 ± 0.051
0.0GlyXaa: 0.0 ± 0.0
His
1.436HisAla: 1.436 ± 0.04
0.191HisCys: 0.191 ± 0.012
1.045HisAsp: 1.045 ± 0.029
1.405HisGlu: 1.405 ± 0.031
1.124HisPhe: 1.124 ± 0.03
1.434HisGly: 1.434 ± 0.041
0.687HisHis: 0.687 ± 0.026
1.704HisIle: 1.704 ± 0.036
1.159HisLys: 1.159 ± 0.03
2.114HisLeu: 2.114 ± 0.047
0.534HisMet: 0.534 ± 0.019
0.789HisAsn: 0.789 ± 0.024
1.157HisPro: 1.157 ± 0.036
0.759HisGln: 0.759 ± 0.024
0.835HisArg: 0.835 ± 0.026
1.445HisSer: 1.445 ± 0.033
1.089HisThr: 1.089 ± 0.03
1.308HisVal: 1.308 ± 0.034
0.197HisTrp: 0.197 ± 0.012
0.899HisTyr: 0.899 ± 0.028
0.0HisXaa: 0.0 ± 0.0
Ile
5.959IleAla: 5.959 ± 0.075
0.694IleCys: 0.694 ± 0.024
3.995IleAsp: 3.995 ± 0.065
5.621IleGlu: 5.621 ± 0.075
3.527IlePhe: 3.527 ± 0.068
5.985IleGly: 5.985 ± 0.091
1.807IleHis: 1.807 ± 0.039
6.178IleIle: 6.178 ± 0.09
5.157IleLys: 5.157 ± 0.075
7.669IleLeu: 7.669 ± 0.106
1.949IleMet: 1.949 ± 0.043
3.262IleAsn: 3.262 ± 0.058
3.41IlePro: 3.41 ± 0.049
2.762IleGln: 2.762 ± 0.052
3.217IleArg: 3.217 ± 0.044
5.978IleSer: 5.978 ± 0.073
4.428IleThr: 4.428 ± 0.06
4.92IleVal: 4.92 ± 0.077
0.646IleTrp: 0.646 ± 0.024
2.482IleTyr: 2.482 ± 0.053
0.0IleXaa: 0.0 ± 0.0
Lys
5.103LysAla: 5.103 ± 0.073
0.331LysCys: 0.331 ± 0.019
4.0LysAsp: 4.0 ± 0.068
6.914LysGlu: 6.914 ± 0.086
2.065LysPhe: 2.065 ± 0.04
4.914LysGly: 4.914 ± 0.069
1.433LysHis: 1.433 ± 0.039
5.178LysIle: 5.178 ± 0.074
6.674LysLys: 6.674 ± 0.095
6.102LysLeu: 6.102 ± 0.071
2.359LysMet: 2.359 ± 0.042
3.869LysAsn: 3.869 ± 0.067
2.361LysPro: 2.361 ± 0.046
2.925LysGln: 2.925 ± 0.057
3.415LysArg: 3.415 ± 0.053
4.184LysSer: 4.184 ± 0.069
4.045LysThr: 4.045 ± 0.064
4.43LysVal: 4.43 ± 0.058
0.828LysTrp: 0.828 ± 0.026
2.262LysTyr: 2.262 ± 0.047
0.0LysXaa: 0.0 ± 0.0
Leu
7.532LeuAla: 7.532 ± 0.105
0.745LeuCys: 0.745 ± 0.026
4.797LeuAsp: 4.797 ± 0.065
6.382LeuGlu: 6.382 ± 0.084
4.775LeuPhe: 4.775 ± 0.094
6.443LeuGly: 6.443 ± 0.092
2.06LeuHis: 2.06 ± 0.037
7.405LeuIle: 7.405 ± 0.095
7.354LeuLys: 7.354 ± 0.085
10.138LeuLeu: 10.138 ± 0.129
2.566LeuMet: 2.566 ± 0.048
4.382LeuAsn: 4.382 ± 0.06
4.049LeuPro: 4.049 ± 0.059
3.554LeuGln: 3.554 ± 0.058
3.59LeuArg: 3.59 ± 0.056
7.336LeuSer: 7.336 ± 0.081
5.287LeuThr: 5.287 ± 0.075
5.521LeuVal: 5.521 ± 0.069
0.852LeuTrp: 0.852 ± 0.032
3.08LeuTyr: 3.08 ± 0.053
0.0LeuXaa: 0.0 ± 0.0
Met
2.147MetAla: 2.147 ± 0.043
0.151MetCys: 0.151 ± 0.011
1.609MetAsp: 1.609 ± 0.035
2.095MetGlu: 2.095 ± 0.051
1.074MetPhe: 1.074 ± 0.032
1.893MetGly: 1.893 ± 0.041
0.51MetHis: 0.51 ± 0.023
2.172MetIle: 2.172 ± 0.044
2.649MetLys: 2.649 ± 0.045
2.655MetLeu: 2.655 ± 0.05
0.807MetMet: 0.807 ± 0.025
1.587MetAsn: 1.587 ± 0.037
1.06MetPro: 1.06 ± 0.028
0.85MetGln: 0.85 ± 0.029
0.983MetArg: 0.983 ± 0.028
1.619MetSer: 1.619 ± 0.038
1.499MetThr: 1.499 ± 0.04
1.655MetVal: 1.655 ± 0.038
0.163MetTrp: 0.163 ± 0.012
0.696MetTyr: 0.696 ± 0.024
0.0MetXaa: 0.0 ± 0.0
Asn
2.73AsnAla: 2.73 ± 0.052
0.299AsnCys: 0.299 ± 0.017
2.153AsnAsp: 2.153 ± 0.042
3.318AsnGlu: 3.318 ± 0.058
1.679AsnPhe: 1.679 ± 0.036
3.441AsnGly: 3.441 ± 0.06
1.042AsnHis: 1.042 ± 0.031
3.528AsnIle: 3.528 ± 0.059
3.121AsnLys: 3.121 ± 0.058
3.886AsnLeu: 3.886 ± 0.062
1.165AsnMet: 1.165 ± 0.031
1.934AsnAsn: 1.934 ± 0.049
2.221AsnPro: 2.221 ± 0.043
1.759AsnGln: 1.759 ± 0.04
1.933AsnArg: 1.933 ± 0.04
2.663AsnSer: 2.663 ± 0.056
2.145AsnThr: 2.145 ± 0.044
2.658AsnVal: 2.658 ± 0.05
0.511AsnTrp: 0.511 ± 0.019
1.462AsnTyr: 1.462 ± 0.034
0.0AsnXaa: 0.0 ± 0.0
Pro
2.848ProAla: 2.848 ± 0.055
0.224ProCys: 0.224 ± 0.013
2.222ProAsp: 2.222 ± 0.044
2.996ProGlu: 2.996 ± 0.057
2.001ProPhe: 2.001 ± 0.044
2.589ProGly: 2.589 ± 0.054
0.829ProHis: 0.829 ± 0.028
2.646ProIle: 2.646 ± 0.046
2.209ProLys: 2.209 ± 0.048
3.546ProLeu: 3.546 ± 0.059
0.789ProMet: 0.789 ± 0.022
1.411ProAsn: 1.411 ± 0.035
1.023ProPro: 1.023 ± 0.031
1.192ProGln: 1.192 ± 0.032
1.105ProArg: 1.105 ± 0.03
2.427ProSer: 2.427 ± 0.045
1.404ProThr: 1.404 ± 0.038
2.924ProVal: 2.924 ± 0.053
0.392ProTrp: 0.392 ± 0.02
1.459ProTyr: 1.459 ± 0.039
0.0ProXaa: 0.0 ± 0.0
Gln
2.623GlnAla: 2.623 ± 0.044
0.207GlnCys: 0.207 ± 0.012
1.632GlnAsp: 1.632 ± 0.034
2.704GlnGlu: 2.704 ± 0.054
1.429GlnPhe: 1.429 ± 0.034
2.279GlnGly: 2.279 ± 0.048
0.75GlnHis: 0.75 ± 0.026
2.458GlnIle: 2.458 ± 0.049
2.747GlnLys: 2.747 ± 0.058
3.549GlnLeu: 3.549 ± 0.054
1.067GlnMet: 1.067 ± 0.031
1.711GlnAsn: 1.711 ± 0.041
1.173GlnPro: 1.173 ± 0.034
1.55GlnGln: 1.55 ± 0.039
1.39GlnArg: 1.39 ± 0.033
2.256GlnSer: 2.256 ± 0.05
1.802GlnThr: 1.802 ± 0.043
2.055GlnVal: 2.055 ± 0.038
0.388GlnTrp: 0.388 ± 0.019
1.322GlnTyr: 1.322 ± 0.034
0.0GlnXaa: 0.0 ± 0.0
Arg
2.473ArgAla: 2.473 ± 0.046
0.273ArgCys: 0.273 ± 0.018
2.142ArgAsp: 2.142 ± 0.047
3.287ArgGlu: 3.287 ± 0.057
1.914ArgPhe: 1.914 ± 0.042
2.424ArgGly: 2.424 ± 0.052
0.84ArgHis: 0.84 ± 0.024
3.163ArgIle: 3.163 ± 0.051
3.329ArgLys: 3.329 ± 0.054
3.992ArgLeu: 3.992 ± 0.056
1.274ArgMet: 1.274 ± 0.035
2.005ArgAsn: 2.005 ± 0.038
1.293ArgPro: 1.293 ± 0.037
1.474ArgGln: 1.474 ± 0.04
1.822ArgArg: 1.822 ± 0.038
2.401ArgSer: 2.401 ± 0.046
1.994ArgThr: 1.994 ± 0.044
2.448ArgVal: 2.448 ± 0.054
0.352ArgTrp: 0.352 ± 0.016
1.542ArgTyr: 1.542 ± 0.038
0.0ArgXaa: 0.0 ± 0.0
Ser
4.604SerAla: 4.604 ± 0.066
0.479SerCys: 0.479 ± 0.022
3.092SerAsp: 3.092 ± 0.055
4.318SerGlu: 4.318 ± 0.06
3.584SerPhe: 3.584 ± 0.06
5.169SerGly: 5.169 ± 0.079
1.405SerHis: 1.405 ± 0.035
5.421SerIle: 5.421 ± 0.076
4.349SerLys: 4.349 ± 0.073
6.965SerLeu: 6.965 ± 0.085
1.89SerMet: 1.89 ± 0.041
2.48SerAsn: 2.48 ± 0.049
2.357SerPro: 2.357 ± 0.043
2.202SerGln: 2.202 ± 0.044
2.688SerArg: 2.688 ± 0.055
4.893SerSer: 4.893 ± 0.082
2.807SerThr: 2.807 ± 0.051
4.286SerVal: 4.286 ± 0.066
0.709SerTrp: 0.709 ± 0.026
2.4SerTyr: 2.4 ± 0.049
0.0SerXaa: 0.0 ± 0.0
Thr
4.456ThrAla: 4.456 ± 0.072
0.349ThrCys: 0.349 ± 0.017
2.551ThrAsp: 2.551 ± 0.043
3.351ThrGlu: 3.351 ± 0.051
2.37ThrPhe: 2.37 ± 0.054
4.369ThrGly: 4.369 ± 0.061
0.963ThrHis: 0.963 ± 0.026
4.063ThrIle: 4.063 ± 0.062
3.198ThrLys: 3.198 ± 0.054
4.905ThrLeu: 4.905 ± 0.065
1.227ThrMet: 1.227 ± 0.031
2.047ThrAsn: 2.047 ± 0.046
2.062ThrPro: 2.062 ± 0.043
1.252ThrGln: 1.252 ± 0.032
1.779ThrArg: 1.779 ± 0.038
3.187ThrSer: 3.187 ± 0.047
2.145ThrThr: 2.145 ± 0.051
3.888ThrVal: 3.888 ± 0.062
0.458ThrTrp: 0.458 ± 0.019
1.679ThrTyr: 1.679 ± 0.038
0.0ThrXaa: 0.0 ± 0.0
Val
4.132ValAla: 4.132 ± 0.066
0.582ValCys: 0.582 ± 0.022
2.758ValAsp: 2.758 ± 0.058
3.692ValGlu: 3.692 ± 0.065
3.193ValPhe: 3.193 ± 0.057
3.667ValGly: 3.667 ± 0.059
1.316ValHis: 1.316 ± 0.038
5.558ValIle: 5.558 ± 0.07
4.693ValLys: 4.693 ± 0.066
6.67ValLeu: 6.67 ± 0.078
1.78ValMet: 1.78 ± 0.041
2.969ValAsn: 2.969 ± 0.05
2.665ValPro: 2.665 ± 0.044
2.201ValGln: 2.201 ± 0.046
2.521ValArg: 2.521 ± 0.049
4.746ValSer: 4.746 ± 0.062
3.694ValThr: 3.694 ± 0.064
3.831ValVal: 3.831 ± 0.06
0.568ValTrp: 0.568 ± 0.024
2.209ValTyr: 2.209 ± 0.043
0.0ValXaa: 0.0 ± 0.0
Trp
0.619TrpAla: 0.619 ± 0.027
0.07TrpCys: 0.07 ± 0.006
0.535TrpAsp: 0.535 ± 0.023
0.609TrpGlu: 0.609 ± 0.02
0.51TrpPhe: 0.51 ± 0.024
0.704TrpGly: 0.704 ± 0.025
0.23TrpHis: 0.23 ± 0.013
0.795TrpIle: 0.795 ± 0.028
0.793TrpLys: 0.793 ± 0.026
1.065TrpLeu: 1.065 ± 0.034
0.322TrpMet: 0.322 ± 0.017
0.547TrpAsn: 0.547 ± 0.021
0.253TrpPro: 0.253 ± 0.015
0.323TrpGln: 0.323 ± 0.016
0.406TrpArg: 0.406 ± 0.017
0.606TrpSer: 0.606 ± 0.023
0.465TrpThr: 0.465 ± 0.02
0.596TrpVal: 0.596 ± 0.023
0.127TrpTrp: 0.127 ± 0.01
0.327TrpTyr: 0.327 ± 0.016
0.0TrpXaa: 0.0 ± 0.0
Tyr
2.081TyrAla: 2.081 ± 0.04
0.346TyrCys: 0.346 ± 0.019
1.82TyrAsp: 1.82 ± 0.047
2.298TyrGlu: 2.298 ± 0.041
1.799TyrPhe: 1.799 ± 0.042
2.555TyrGly: 2.555 ± 0.05
0.871TyrHis: 0.871 ± 0.028
2.433TyrIle: 2.433 ± 0.046
2.326TyrLys: 2.326 ± 0.047
3.526TyrLeu: 3.526 ± 0.056
0.867TyrMet: 0.867 ± 0.029
1.429TyrAsn: 1.429 ± 0.034
1.383TyrPro: 1.383 ± 0.034
1.491TyrGln: 1.491 ± 0.039
1.601TyrArg: 1.601 ± 0.034
2.364TyrSer: 2.364 ± 0.04
1.821TyrThr: 1.821 ± 0.037
1.96TyrVal: 1.96 ± 0.041
0.398TyrTrp: 0.398 ± 0.019
1.395TyrTyr: 1.395 ± 0.041
0.0TyrXaa: 0.0 ± 0.0
Xaa
0.0XaaAla: 0.0 ± 0.0
0.0XaaCys: 0.0 ± 0.0
0.0XaaAsp: 0.0 ± 0.0
0.0XaaGlu: 0.0 ± 0.0
0.0XaaPhe: 0.0 ± 0.0
0.0XaaGly: 0.0 ± 0.0
0.0XaaHis: 0.0 ± 0.0
0.0XaaIle: 0.0 ± 0.0
0.0XaaLys: 0.0 ± 0.0
0.0XaaLeu: 0.0 ± 0.0
0.0XaaMet: 0.0 ± 0.0
0.0XaaAsn: 0.0 ± 0.0
0.0XaaPro: 0.0 ± 0.0
0.0XaaGln: 0.0 ± 0.0
0.0XaaArg: 0.0 ± 0.0
0.0XaaSer: 0.0 ± 0.0
0.0XaaThr: 0.0 ± 0.0
0.0XaaVal: 0.0 ± 0.0
0.0XaaTrp: 0.0 ± 0.0
0.0XaaTyr: 0.0 ± 0.0
0.0XaaXaa: 0.0 ± 0.0
Statistics based on 4336 proteins (1222119 amino acids)

Note: The error has been estimated with the bootstraping (x100) at the protein level

Above dipeptide statistics (among other stats for this proteome) you can download from this CSV file
See this proteome in: uniprot_link
Proteome-pI is available under Creative Commons Attribution-NoDerivs license, for more details see here

Reference: Kozlowski LP. Proteome-pI 2.0: Proteome Isoelectric Point Database Update. Nucleic Acids Res. 2021, doi: 10.1093/nar/gkab944 Contact: Lukasz P. Kozlowski