Delphinus delphis polyomavirus 1
Average proteome isoelectric point is 6.77
Get precalculated fractions of proteins
Acidic
pI < 6.8
6.8-7.4
pI > 7.4
Basic
All
Note: above files contain also dissociation constants (pKa)
Virtual 2D-PAGE plot for 5 proteins (isoelectric point calculated using IPC2_protein)
Get csv file with sequences according to given criteria:
* You can choose from 21 different methods for calculating isoelectric point
Summary statistics related to proteome-wise predictions
Protein with the lowest isoelectric point:
>tr|S5M639|S5M639_9POLY Minor capsid protein OS=Delphinus delphis polyomavirus 1 OX=1891756 PE=3 SV=1
MM1 pKa = 7.52 SARR4 pKa = 11.84 KK5 pKa = 9.68 GRR7 pKa = 11.84 GAVRR11 pKa = 11.84 PPSQVPKK18 pKa = 10.4 LIVKK22 pKa = 10.38 GGVEE26 pKa = 4.02 VLGVKK31 pKa = 9.28 TGPDD35 pKa = 3.15 SILYY39 pKa = 8.33 VEE41 pKa = 5.91 CYY43 pKa = 9.4 LQPRR47 pKa = 11.84 MGDD50 pKa = 3.33 PATNGASSGQLTRR63 pKa = 11.84 NSTNEE68 pKa = 3.95 TDD70 pKa = 4.85 NEE72 pKa = 4.47 TQQGTRR78 pKa = 11.84 LARR81 pKa = 11.84 YY82 pKa = 8.92 SCGSIQLPLLNDD94 pKa = 3.91 DD95 pKa = 4.06 MTQGTILMWEE105 pKa = 3.94 AFEE108 pKa = 4.92 VKK110 pKa = 9.94 TEE112 pKa = 3.92 LVGINVLTNFHH123 pKa = 6.31 SAEE126 pKa = 3.99 DD127 pKa = 3.99 LAWPDD132 pKa = 3.89 GPGLPIQGLNFHH144 pKa = 6.69 MFAVGGQPLEE154 pKa = 4.07 LQGILMNHH162 pKa = 6.5 LTTYY166 pKa = 10.06 PDD168 pKa = 3.6 DD169 pKa = 3.66 VVVPNRR175 pKa = 11.84 TNKK178 pKa = 9.08 TPSLKK183 pKa = 10.85 VLDD186 pKa = 4.24 PTAKK190 pKa = 10.34 SQLTEE195 pKa = 4.45 DD196 pKa = 3.39 GKK198 pKa = 11.4 FPIEE202 pKa = 4.36 CWAPDD207 pKa = 3.61 PSKK210 pKa = 11.5 NEE212 pKa = 3.61 NTRR215 pKa = 11.84 YY216 pKa = 9.51 FCSLTGGTQTPPVLQQTNTVTTVLLDD242 pKa = 3.84 ANGVGPLCKK251 pKa = 9.67 GDD253 pKa = 3.66 RR254 pKa = 11.84 LFVTSADD261 pKa = 3.16 IIGIHH266 pKa = 6.84 KK267 pKa = 10.04 DD268 pKa = 3.34 ASHH271 pKa = 6.46 HH272 pKa = 5.75 AFLRR276 pKa = 11.84 GLPRR280 pKa = 11.84 FFRR283 pKa = 11.84 VGLRR287 pKa = 11.84 KK288 pKa = 8.79 RR289 pKa = 11.84 TVKK292 pKa = 10.55 NPYY295 pKa = 9.03 PVYY298 pKa = 11.4 SMLNSLFTNLNPSITGQSMQGANSQVEE325 pKa = 4.54 EE326 pKa = 4.11 VSVYY330 pKa = 10.56 QGTEE334 pKa = 3.96 PLPADD339 pKa = 3.75 PDD341 pKa = 3.32 MTRR344 pKa = 11.84 FINEE348 pKa = 4.53 FGQEE352 pKa = 4.21 TTSLPHH358 pKa = 6.28
Molecular weight: 39.07 kDa
Isoelectric point according different methods:
IPC2.protein.svr19 5.611
IPC2_protein 5.588
IPC_protein 5.588
Toseland 5.919
ProMoST 5.753
Dawson 5.804
Bjellqvist 5.83
Wikipedia 5.792
Rodwell 5.779
Grimsley 6.046
Solomon 5.804
Lehninger 5.792
Nozaki 6.046
DTASelect 6.237
Thurlkill 6.198
EMBOSS 6.16
Sillero 6.148
Patrickios 4.202
IPC_peptide 5.817
IPC2_peptide 6.148
IPC2.peptide.svr19 6.147
Protein with the highest isoelectric point:
>tr|S5MND0|S5MND0_9POLY Small T antigen OS=Delphinus delphis polyomavirus 1 OX=1891756 PE=4 SV=1
MM1 pKa = 7.49 ALVEE5 pKa = 4.45 WRR7 pKa = 11.84 PEE9 pKa = 4.2 LIDD12 pKa = 3.52 YY13 pKa = 7.92 NFPGVRR19 pKa = 11.84 WLARR23 pKa = 11.84 NVEE26 pKa = 4.28 YY27 pKa = 10.54 FDD29 pKa = 3.6 PRR31 pKa = 11.84 FWASEE36 pKa = 3.62 LWRR39 pKa = 11.84 VFMEE43 pKa = 4.34 SVARR47 pKa = 11.84 EE48 pKa = 3.7 GAAQIEE54 pKa = 4.43 AASTALAEE62 pKa = 4.35 QGRR65 pKa = 11.84 SVAADD70 pKa = 4.5 AIARR74 pKa = 11.84 SLEE77 pKa = 3.75 NANWVISEE85 pKa = 4.3 TGALARR91 pKa = 11.84 RR92 pKa = 11.84 GLDD95 pKa = 3.04 VTGALVRR102 pKa = 11.84 QAGQRR107 pKa = 11.84 AVQSTGSIITNAYY120 pKa = 7.79 TNLGDD125 pKa = 3.97 YY126 pKa = 10.82 YY127 pKa = 10.97 RR128 pKa = 11.84 GLTPLRR134 pKa = 11.84 PPQRR138 pKa = 11.84 RR139 pKa = 11.84 AMLRR143 pKa = 11.84 EE144 pKa = 3.99 LEE146 pKa = 4.08 KK147 pKa = 10.84 SEE149 pKa = 3.96 RR150 pKa = 11.84 QQTITGEE157 pKa = 4.4 VVYY160 pKa = 10.21 KK161 pKa = 10.92 APAPGGANQRR171 pKa = 11.84 ITPEE175 pKa = 3.49 WMLPLILGLYY185 pKa = 7.75 NHH187 pKa = 6.75 PTWGVPDD194 pKa = 3.96 AVTALKK200 pKa = 10.59 SSQEE204 pKa = 3.89 KK205 pKa = 10.31 EE206 pKa = 3.98 EE207 pKa = 4.1 NVSKK211 pKa = 10.57 KK212 pKa = 9.96 RR213 pKa = 11.84 KK214 pKa = 9.11 RR215 pKa = 11.84 SCEE218 pKa = 3.93 TTQPGAKK225 pKa = 8.73 TNCKK229 pKa = 9.24 RR230 pKa = 11.84 RR231 pKa = 11.84 RR232 pKa = 11.84 RR233 pKa = 11.84 STRR236 pKa = 11.84 RR237 pKa = 3.09
Molecular weight: 26.64 kDa
Isoelectric point according different methods:
IPC2.protein.svr19 9.265
IPC2_protein 9.326
IPC_protein 9.736
Toseland 10.058
ProMoST 9.926
Dawson 10.277
Bjellqvist 9.999
Wikipedia 10.482
Rodwell 10.467
Grimsley 10.35
Solomon 10.321
Lehninger 10.292
Nozaki 10.072
DTASelect 9.984
Thurlkill 10.116
EMBOSS 10.467
Sillero 10.189
Patrickios 9.955
IPC_peptide 10.321
IPC2_peptide 8.829
IPC2.peptide.svr19 8.48
Peptides (in silico digests for buttom-up proteomics)
Below you can find
in silico digests of the whole proteome with Trypsin, Chymotrypsin, Trypsin+LysC, LysN, ArgC proteases suitable for different mass spec machines.
Try ESI
ChTry ESI
ArgC ESI
LysN ESI
TryLysC ESI
Try MALDI
ChTry MALDI
ArgC MALDI
LysN MALDI
TryLysC MALDI
Try LTQ
ChTry LTQ
ArgC LTQ
LysN LTQ
TryLysC LTQ
Try MSlow
ChTry MSlow
ArgC MSlow
LysN MSlow
TryLysC MSlow
Try MShigh
ChTry MShigh
ArgC MShigh
LysN MShigh
TryLysC MShigh
General Statistics
Number of major isoforms
Number of additional isoforms
Number of all proteins
Number of amino acids
Min. Seq. Length
Max. Seq. Length
Avg. Seq. Length
Avg. Mol. Weight
5
0
5
1746
169
634
349.2
39.02
Amino acid frequency
Ala
Cys
Asp
Glu
Phe
Gly
His
Ile
Lys
Leu
7.446 ± 1.494
2.577 ± 0.698
4.926 ± 0.732
6.529 ± 0.591
3.608 ± 0.475
6.987 ± 0.591
1.775 ± 0.366
4.811 ± 0.883
5.785 ± 0.975
10.481 ± 0.678
Met
Asn
Gln
Pro
Arg
Ser
Thr
Val
Trp
Tyr
2.577 ± 0.391
5.04 ± 0.406
5.212 ± 0.665
3.952 ± 0.365
5.556 ± 1.184
5.727 ± 0.22
7.045 ± 0.515
5.498 ± 0.548
1.718 ± 0.395
2.749 ± 0.162
Note: For amino acid frequency statistics the error has been estimated with the bootstraping (x100) at the protein level
Most of the basic statistics you can see at this page can be downloaded from this CSV file
For dipeptide frequency statistics click here