Listeria phage PSU-VKH-LP019

Taxonomy: Viruses; Duplodnaviria; Heunggongvirae; Uroviricota; Caudoviricetes; Caudovirales; Siphoviridae; unclassified Siphoviridae

Average proteome isoelectric point is 6.05

Get precalculated fractions of proteins

Acidic
pI < 6.8
6.8-7.4
pI > 7.4
Basic
    
All



Virtual 2D-PAGE plot for 66 proteins (isoelectric point calculated using IPC2_protein)

Get csv file with sequences according to given criteria:
        -     Method 

     -  kDa    
                                                                                      

* You can choose from 21 different methods for calculating isoelectric point

Summary statistics related to proteome-wise predictions


    
Protein with the lowest isoelectric point:
>tr|A0A2U8UUN7|A0A2U8UUN7_9CAUD Uncharacterized protein OS=Listeria phage PSU-VKH-LP019 OX=2202246 PE=4 SV=1
MM1 pKa = 7.8PGLIAKK7 pKa = 8.89QPNGLYY13 pKa = 10.33CRR15 pKa = 11.84ISTVVEE21 pKa = 4.47APTHH25 pKa = 6.66DD26 pKa = 3.61NMTKK30 pKa = 10.66EE31 pKa = 4.15EE32 pKa = 4.79LEE34 pKa = 4.55DD35 pKa = 3.88LLITQRR41 pKa = 11.84SLDD44 pKa = 3.72INFVALDD51 pKa = 3.28QWLAVYY57 pKa = 9.79EE58 pKa = 4.07VDD60 pKa = 4.01FNVAIEE66 pKa = 4.21EE67 pKa = 4.37LGSACSTFEE76 pKa = 3.91EE77 pKa = 4.4TKK79 pKa = 10.23EE80 pKa = 3.68WLEE83 pKa = 3.86EE84 pKa = 3.74VGYY87 pKa = 9.68QQADD91 pKa = 3.25VFMEE95 pKa = 4.68KK96 pKa = 9.53IAYY99 pKa = 8.94NWDD102 pKa = 2.96EE103 pKa = 4.1WEE105 pKa = 4.55EE106 pKa = 3.88EE107 pKa = 4.02DD108 pKa = 5.64DD109 pKa = 4.25

Molecular weight: 12.66 kDa
Isoelectric point according different methods:





Protein with the highest isoelectric point:
>tr|A0A2U8UUN0|A0A2U8UUN0_9CAUD Putative single-stranded DNA-binding protein OS=Listeria phage PSU-VKH-LP019 OX=2202246 PE=3 SV=1
MM1 pKa = 7.43GKK3 pKa = 10.33YY4 pKa = 8.07YY5 pKa = 10.08WHH7 pKa = 6.92VSRR10 pKa = 11.84LGGKK14 pKa = 6.75PTEE17 pKa = 3.88IRR19 pKa = 11.84HH20 pKa = 5.44YY21 pKa = 10.3NHH23 pKa = 5.92ITKK26 pKa = 9.45MYY28 pKa = 10.54KK29 pKa = 10.34FILRR33 pKa = 11.84NPAMFKK39 pKa = 10.89DD40 pKa = 3.7KK41 pKa = 10.43TLTIYY46 pKa = 10.78NHH48 pKa = 6.44AKK50 pKa = 10.28AVTNMTFNEE59 pKa = 3.72IKK61 pKa = 10.7YY62 pKa = 9.87RR63 pKa = 11.84ASLNLCEE70 pKa = 4.31TVEE73 pKa = 3.87RR74 pKa = 11.84RR75 pKa = 11.84YY76 pKa = 10.5VLSLTQRR83 pKa = 11.84LKK85 pKa = 11.07EE86 pKa = 4.04EE87 pKa = 4.13QAA89 pKa = 3.46

Molecular weight: 10.7 kDa
Isoelectric point according different methods:





Peptides (in silico digests for buttom-up proteomics)

Below you can find in silico digests of the whole proteome with Trypsin, Chymotrypsin, Trypsin+LysC, LysN, ArgC proteases suitable for different mass spec machines.

Try
ESI
ChTry
ESI
ArgC
ESI
LysN
ESI
TryLysC
ESI

Try
MALDI
ChTry
MALDI
ArgC
MALDI
LysN
MALDI
TryLysC
MALDI

Try
LTQ
ChTry
LTQ
ArgC
LTQ
LysN
LTQ
TryLysC
LTQ

Try
MSlow
ChTry
MSlow
ArgC
MSlow
LysN
MSlow
TryLysC
MSlow

Try
MShigh
ChTry
MShigh
ArgC
MShigh
LysN
MShigh
TryLysC
MShigh

General Statistics

Number of major isoforms

Number of additional isoforms

Number of all proteins

Number of amino acids

Min. Seq. Length

Max. Seq. Length

Avg. Seq. Length

Avg. Mol. Weight

66

0

66

12091

41

1599

183.2

20.82

Amino acid frequency

Ala

Cys

Asp

Glu

Phe

Gly

His

Ile

Lys

Leu

6.575 ± 0.57

0.885 ± 0.165

5.955 ± 0.262

8.519 ± 0.564

3.962 ± 0.286

5.732 ± 0.313

1.373 ± 0.175

7.253 ± 0.34

9.321 ± 0.404

7.791 ± 0.259

Met

Asn

Gln

Pro

Arg

Ser

Thr

Val

Trp

Tyr

2.531 ± 0.142

6.534 ± 0.28

2.614 ± 0.174

3.929 ± 0.217

3.73 ± 0.283

6.253 ± 0.288

5.955 ± 0.306

6.004 ± 0.246

1.15 ± 0.123

3.937 ± 0.246
Note: For amino acid frequency statistics the error has been estimated with the bootstraping (x100) at the protein level

Most of the basic statistics you can see at this page can be downloaded from this CSV file

For dipeptide frequency statistics click here
See this proteome in: uniprot_link
Proteome-pI is available under Creative Commons Attribution-NoDerivs license, for more details see here

Reference: Kozlowski LP. Proteome-pI 2.0: Proteome Isoelectric Point Database Update. Nucleic Acids Res. 2021, doi: 10.1093/nar/gkab944 Contact: Lukasz P. Kozlowski