Giant panda associated partiti-like virus
Average proteome isoelectric point is 8.59
Get precalculated fractions of proteins
Acidic
pI < 6.8
6.8-7.4
pI > 7.4
Basic
All
Note: above files contain also dissociation constants (pKa)
Virtual 2D-PAGE plot for 1 proteins (isoelectric point calculated using IPC2_protein)
Get csv file with sequences according to given criteria:
* You can choose from 21 different methods for calculating isoelectric point
Summary statistics related to proteome-wise predictions
Protein with the lowest isoelectric point:
>tr|A0A220IGT0|A0A220IGT0_9VIRU RdRp OS=Giant panda associated partiti-like virus OX=2016462 PE=4 SV=1
MM1 pKa = 6.82 EE2 pKa = 5.88 HH3 pKa = 7.31 SMKK6 pKa = 9.58 TWHH9 pKa = 6.91 KK10 pKa = 10.5 NLDD13 pKa = 3.83 KK14 pKa = 10.92 EE15 pKa = 4.71 SSRR18 pKa = 11.84 EE19 pKa = 3.72 DD20 pKa = 3.47 DD21 pKa = 4.13 YY22 pKa = 12.05 QPVIYY27 pKa = 9.28 TLSRR31 pKa = 11.84 EE32 pKa = 4.1 IVRR35 pKa = 11.84 EE36 pKa = 3.6 RR37 pKa = 11.84 STQLNLNQEE46 pKa = 4.34 VTSLQEE52 pKa = 3.91 GPYY55 pKa = 8.12 MWKK58 pKa = 8.2 TVPYY62 pKa = 9.4 KK63 pKa = 10.58 LAALRR68 pKa = 11.84 EE69 pKa = 4.27 WNLRR73 pKa = 11.84 MSRR76 pKa = 11.84 SEE78 pKa = 3.88 TPEE81 pKa = 3.43 IFDD84 pKa = 3.63 PVVAHH89 pKa = 6.46 VLRR92 pKa = 11.84 EE93 pKa = 3.82 QGFEE97 pKa = 3.99 VDD99 pKa = 3.13 SKK101 pKa = 11.35 KK102 pKa = 10.77 YY103 pKa = 8.9 YY104 pKa = 9.96 EE105 pKa = 4.19 PRR107 pKa = 11.84 SVYY110 pKa = 10.35 SVEE113 pKa = 4.55 KK114 pKa = 10.59 FFEE117 pKa = 4.15 QLQSFNPSKK126 pKa = 11.0 SVFVDD131 pKa = 3.35 VKK133 pKa = 10.75 DD134 pKa = 3.7 RR135 pKa = 11.84 HH136 pKa = 4.72 VQRR139 pKa = 11.84 GIAFAYY145 pKa = 9.72 KK146 pKa = 10.45 VFGKK150 pKa = 10.82 GKK152 pKa = 9.93 DD153 pKa = 3.51 DD154 pKa = 4.75 AYY156 pKa = 11.34 LEE158 pKa = 4.24 PLEE161 pKa = 5.09 LWDD164 pKa = 5.1 EE165 pKa = 4.54 SIISNWKK172 pKa = 9.1 GSAGVTAYY180 pKa = 10.4 GQTKK184 pKa = 9.8 RR185 pKa = 11.84 EE186 pKa = 4.05 AFSRR190 pKa = 11.84 GILSAKK196 pKa = 10.0 RR197 pKa = 11.84 ILGQDD202 pKa = 2.97 GLARR206 pKa = 11.84 RR207 pKa = 11.84 KK208 pKa = 9.78 PEE210 pKa = 3.94 PCLGLTRR217 pKa = 11.84 TGKK220 pKa = 9.93 NGKK223 pKa = 9.11 SRR225 pKa = 11.84 LIWGYY230 pKa = 9.24 PMSMTLIEE238 pKa = 4.64 GSLAKK243 pKa = 10.1 PLLGTLTGLNTPMAYY258 pKa = 9.92 KK259 pKa = 9.42 LTNKK263 pKa = 8.48 TLGMKK268 pKa = 9.9 ILAASQTKK276 pKa = 8.73 KK277 pKa = 9.36 WYY279 pKa = 9.49 YY280 pKa = 11.03 SLDD283 pKa = 3.22 ATQFDD288 pKa = 4.22 SSIQKK293 pKa = 10.16 IMIEE297 pKa = 4.09 VAFSIIATHH306 pKa = 6.55 FDD308 pKa = 3.35 RR309 pKa = 11.84 KK310 pKa = 10.83 AMYY313 pKa = 10.66 DD314 pKa = 3.45 DD315 pKa = 4.14 VNTVGEE321 pKa = 4.18 VLEE324 pKa = 4.18 LVRR327 pKa = 11.84 DD328 pKa = 4.05 YY329 pKa = 11.04 FIHH332 pKa = 6.16 TPIVMPAGEE341 pKa = 4.18 KK342 pKa = 10.75 AKK344 pKa = 11.14 GNVQGVLYY352 pKa = 9.86 YY353 pKa = 10.46 GKK355 pKa = 10.15 KK356 pKa = 9.76 HH357 pKa = 5.35 GVPSGSYY364 pKa = 7.69 FTQLVDD370 pKa = 3.99 SIVNIIVLGTLASKK384 pKa = 10.71 FGFYY388 pKa = 10.42 LSKK391 pKa = 11.36 EE392 pKa = 4.3 EE393 pKa = 4.26 IFVLGDD399 pKa = 3.44 DD400 pKa = 4.95 LLFFTNTHH408 pKa = 6.57 LDD410 pKa = 3.35 VKK412 pKa = 10.74 KK413 pKa = 10.48 PSTSYY418 pKa = 11.18 AKK420 pKa = 9.49 STFGMLLKK428 pKa = 10.78
Molecular weight: 48.63 kDa
Isoelectric point according different methods:
IPC2.protein.svr19 8.593
IPC2_protein 8.595
IPC_protein 8.478
Toseland 9.151
ProMoST 9.019
Dawson 9.458
Bjellqvist 9.165
Wikipedia 9.648
Rodwell 9.692
Grimsley 9.56
Solomon 9.502
Lehninger 9.458
Nozaki 9.165
DTASelect 9.151
Thurlkill 9.282
EMBOSS 9.589
Sillero 9.385
Patrickios 4.647
IPC_peptide 9.487
IPC2_peptide 7.644
IPC2.peptide.svr19 7.654
Protein with the highest isoelectric point:
>tr|A0A220IGT0|A0A220IGT0_9VIRU RdRp OS=Giant panda associated partiti-like virus OX=2016462 PE=4 SV=1
MM1 pKa = 6.82 EE2 pKa = 5.88 HH3 pKa = 7.31 SMKK6 pKa = 9.58 TWHH9 pKa = 6.91 KK10 pKa = 10.5 NLDD13 pKa = 3.83 KK14 pKa = 10.92 EE15 pKa = 4.71 SSRR18 pKa = 11.84 EE19 pKa = 3.72 DD20 pKa = 3.47 DD21 pKa = 4.13 YY22 pKa = 12.05 QPVIYY27 pKa = 9.28 TLSRR31 pKa = 11.84 EE32 pKa = 4.1 IVRR35 pKa = 11.84 EE36 pKa = 3.6 RR37 pKa = 11.84 STQLNLNQEE46 pKa = 4.34 VTSLQEE52 pKa = 3.91 GPYY55 pKa = 8.12 MWKK58 pKa = 8.2 TVPYY62 pKa = 9.4 KK63 pKa = 10.58 LAALRR68 pKa = 11.84 EE69 pKa = 4.27 WNLRR73 pKa = 11.84 MSRR76 pKa = 11.84 SEE78 pKa = 3.88 TPEE81 pKa = 3.43 IFDD84 pKa = 3.63 PVVAHH89 pKa = 6.46 VLRR92 pKa = 11.84 EE93 pKa = 3.82 QGFEE97 pKa = 3.99 VDD99 pKa = 3.13 SKK101 pKa = 11.35 KK102 pKa = 10.77 YY103 pKa = 8.9 YY104 pKa = 9.96 EE105 pKa = 4.19 PRR107 pKa = 11.84 SVYY110 pKa = 10.35 SVEE113 pKa = 4.55 KK114 pKa = 10.59 FFEE117 pKa = 4.15 QLQSFNPSKK126 pKa = 11.0 SVFVDD131 pKa = 3.35 VKK133 pKa = 10.75 DD134 pKa = 3.7 RR135 pKa = 11.84 HH136 pKa = 4.72 VQRR139 pKa = 11.84 GIAFAYY145 pKa = 9.72 KK146 pKa = 10.45 VFGKK150 pKa = 10.82 GKK152 pKa = 9.93 DD153 pKa = 3.51 DD154 pKa = 4.75 AYY156 pKa = 11.34 LEE158 pKa = 4.24 PLEE161 pKa = 5.09 LWDD164 pKa = 5.1 EE165 pKa = 4.54 SIISNWKK172 pKa = 9.1 GSAGVTAYY180 pKa = 10.4 GQTKK184 pKa = 9.8 RR185 pKa = 11.84 EE186 pKa = 4.05 AFSRR190 pKa = 11.84 GILSAKK196 pKa = 10.0 RR197 pKa = 11.84 ILGQDD202 pKa = 2.97 GLARR206 pKa = 11.84 RR207 pKa = 11.84 KK208 pKa = 9.78 PEE210 pKa = 3.94 PCLGLTRR217 pKa = 11.84 TGKK220 pKa = 9.93 NGKK223 pKa = 9.11 SRR225 pKa = 11.84 LIWGYY230 pKa = 9.24 PMSMTLIEE238 pKa = 4.64 GSLAKK243 pKa = 10.1 PLLGTLTGLNTPMAYY258 pKa = 9.92 KK259 pKa = 9.42 LTNKK263 pKa = 8.48 TLGMKK268 pKa = 9.9 ILAASQTKK276 pKa = 8.73 KK277 pKa = 9.36 WYY279 pKa = 9.49 YY280 pKa = 11.03 SLDD283 pKa = 3.22 ATQFDD288 pKa = 4.22 SSIQKK293 pKa = 10.16 IMIEE297 pKa = 4.09 VAFSIIATHH306 pKa = 6.55 FDD308 pKa = 3.35 RR309 pKa = 11.84 KK310 pKa = 10.83 AMYY313 pKa = 10.66 DD314 pKa = 3.45 DD315 pKa = 4.14 VNTVGEE321 pKa = 4.18 VLEE324 pKa = 4.18 LVRR327 pKa = 11.84 DD328 pKa = 4.05 YY329 pKa = 11.04 FIHH332 pKa = 6.16 TPIVMPAGEE341 pKa = 4.18 KK342 pKa = 10.75 AKK344 pKa = 11.14 GNVQGVLYY352 pKa = 9.86 YY353 pKa = 10.46 GKK355 pKa = 10.15 KK356 pKa = 9.76 HH357 pKa = 5.35 GVPSGSYY364 pKa = 7.69 FTQLVDD370 pKa = 3.99 SIVNIIVLGTLASKK384 pKa = 10.71 FGFYY388 pKa = 10.42 LSKK391 pKa = 11.36 EE392 pKa = 4.3 EE393 pKa = 4.26 IFVLGDD399 pKa = 3.44 DD400 pKa = 4.95 LLFFTNTHH408 pKa = 6.57 LDD410 pKa = 3.35 VKK412 pKa = 10.74 KK413 pKa = 10.48 PSTSYY418 pKa = 11.18 AKK420 pKa = 9.49 STFGMLLKK428 pKa = 10.78
Molecular weight: 48.63 kDa
Isoelectric point according different methods:
IPC2.protein.svr19 8.593
IPC2_protein 8.595
IPC_protein 8.478
Toseland 9.151
ProMoST 9.019
Dawson 9.458
Bjellqvist 9.165
Wikipedia 9.648
Rodwell 9.692
Grimsley 9.56
Solomon 9.502
Lehninger 9.458
Nozaki 9.165
DTASelect 9.151
Thurlkill 9.282
EMBOSS 9.589
Sillero 9.385
Patrickios 4.647
IPC_peptide 9.487
IPC2_peptide 7.644
IPC2.peptide.svr19 7.654
Peptides (in silico digests for buttom-up proteomics)
Below you can find
in silico digests of the whole proteome with Trypsin, Chymotrypsin, Trypsin+LysC, LysN, ArgC proteases suitable for different mass spec machines.
Try ESI
ChTry ESI
ArgC ESI
LysN ESI
TryLysC ESI
Try MALDI
ChTry MALDI
ArgC MALDI
LysN MALDI
TryLysC MALDI
Try LTQ
ChTry LTQ
ArgC LTQ
LysN LTQ
TryLysC LTQ
Try MSlow
ChTry MSlow
ArgC MSlow
LysN MSlow
TryLysC MSlow
Try MShigh
ChTry MShigh
ArgC MShigh
LysN MShigh
TryLysC MShigh
General Statistics
Number of major isoforms
Number of additional isoforms
Number of all proteins
Number of amino acids
Min. Seq. Length
Max. Seq. Length
Avg. Seq. Length
Avg. Mol. Weight
1
0
1
428
428
428
428.0
48.63
Amino acid frequency
Ala
Cys
Asp
Glu
Phe
Gly
His
Ile
Lys
Leu
5.374 ± 0.0
0.234 ± 0.0
4.907 ± 0.0
6.308 ± 0.0
4.673 ± 0.0
7.009 ± 0.0
1.869 ± 0.0
5.14 ± 0.0
8.645 ± 0.0
9.813 ± 0.0
Met
Asn
Gln
Pro
Arg
Ser
Thr
Val
Trp
Tyr
2.804 ± 0.0
3.037 ± 0.0
3.972 ± 0.0
3.505 ± 0.0
4.673 ± 0.0
8.178 ± 0.0
6.308 ± 0.0
7.009 ± 0.0
1.636 ± 0.0
4.907 ± 0.0
Note: For amino acid frequency statistics the error has been estimated with the bootstraping (x100) at the protein level
Most of the basic statistics you can see at this page can be downloaded from this CSV file
For dipeptide frequency statistics click here