Amino acid dipepetide frequency for Comamonas sp. NLF-7-7

Apart from single amino acid frequencies one can also calculate so called amino acid dipeptide frequency.

There is 400 possibilites (441 if we consider Xaa as additional 21st amino acid representing all non-standard or unknown amino acids). Thus, if the dipeptides would be present randomly in proteins each amino acid dipeptide should be present with 0.25% frequency. As it is not the case in the nature, for better visablity all more than expected dipepetides are marked by red, and those which are underrepresented are marked by blue in the table.

All values are presented as per milles (‰), therefore need to be multiplied by 10-3.

For more information see sequence space article on Wikipedia.

AlaCysAspGluPheGlyHisIleLysLeuMetAsnProGlnArgSerThrValTrpTyrXaa
Ala
19.595AlaAla: 19.595 ± 0.22
1.503AlaCys: 1.503 ± 0.042
6.3AlaAsp: 6.3 ± 0.085
6.828AlaGlu: 6.828 ± 0.104
3.947AlaPhe: 3.947 ± 0.073
11.567AlaGly: 11.567 ± 0.098
3.155AlaHis: 3.155 ± 0.063
5.117AlaIle: 5.117 ± 0.083
3.05AlaLys: 3.05 ± 0.068
17.616AlaLeu: 17.616 ± 0.2
3.651AlaMet: 3.651 ± 0.058
2.511AlaAsn: 2.511 ± 0.064
7.4AlaPro: 7.4 ± 0.127
7.599AlaGln: 7.599 ± 0.103
11.269AlaArg: 11.269 ± 0.146
7.012AlaSer: 7.012 ± 0.085
5.897AlaThr: 5.897 ± 0.082
8.993AlaVal: 8.993 ± 0.116
2.286AlaTrp: 2.286 ± 0.064
2.363AlaTyr: 2.363 ± 0.046
0.0AlaXaa: 0.0 ± 0.0
Cys
1.327CysAla: 1.327 ± 0.041
0.139CysCys: 0.139 ± 0.012
0.5CysAsp: 0.5 ± 0.025
0.52CysGlu: 0.52 ± 0.025
0.29CysPhe: 0.29 ± 0.017
0.921CysGly: 0.921 ± 0.032
0.277CysHis: 0.277 ± 0.016
0.404CysIle: 0.404 ± 0.02
0.219CysLys: 0.219 ± 0.016
0.893CysLeu: 0.893 ± 0.032
0.222CysMet: 0.222 ± 0.015
0.218CysAsn: 0.218 ± 0.015
0.524CysPro: 0.524 ± 0.026
0.275CysGln: 0.275 ± 0.017
0.566CysArg: 0.566 ± 0.027
0.496CysSer: 0.496 ± 0.024
0.535CysThr: 0.535 ± 0.026
0.717CysVal: 0.717 ± 0.031
0.139CysTrp: 0.139 ± 0.012
0.199CysTyr: 0.199 ± 0.015
0.0CysXaa: 0.0 ± 0.0
Asp
7.499AspAla: 7.499 ± 0.1
0.44AspCys: 0.44 ± 0.021
2.339AspAsp: 2.339 ± 0.055
3.328AspGlu: 3.328 ± 0.059
1.948AspPhe: 1.948 ± 0.047
4.461AspGly: 4.461 ± 0.066
0.972AspHis: 0.972 ± 0.034
2.239AspIle: 2.239 ± 0.051
1.53AspLys: 1.53 ± 0.048
4.89AspLeu: 4.89 ± 0.078
1.147AspMet: 1.147 ± 0.034
1.018AspAsn: 1.018 ± 0.035
2.586AspPro: 2.586 ± 0.049
1.472AspGln: 1.472 ± 0.043
2.673AspArg: 2.673 ± 0.065
2.067AspSer: 2.067 ± 0.045
2.326AspThr: 2.326 ± 0.043
3.605AspVal: 3.605 ± 0.064
1.031AspTrp: 1.031 ± 0.031
1.44AspTyr: 1.44 ± 0.038
0.0AspXaa: 0.0 ± 0.0
Glu
6.712GluAla: 6.712 ± 0.103
0.396GluCys: 0.396 ± 0.021
2.324GluAsp: 2.324 ± 0.056
2.46GluGlu: 2.46 ± 0.064
1.624GluPhe: 1.624 ± 0.048
3.945GluGly: 3.945 ± 0.064
1.587GluHis: 1.587 ± 0.044
2.536GluIle: 2.536 ± 0.055
1.725GluLys: 1.725 ± 0.056
6.308GluLeu: 6.308 ± 0.088
1.171GluMet: 1.171 ± 0.035
1.123GluAsn: 1.123 ± 0.035
2.697GluPro: 2.697 ± 0.059
2.943GluGln: 2.943 ± 0.063
5.202GluArg: 5.202 ± 0.073
2.222GluSer: 2.222 ± 0.045
2.226GluThr: 2.226 ± 0.052
4.107GluVal: 4.107 ± 0.07
0.65GluTrp: 0.65 ± 0.026
0.999GluTyr: 0.999 ± 0.032
0.0GluXaa: 0.0 ± 0.0
Phe
4.292PheAla: 4.292 ± 0.06
0.345PheCys: 0.345 ± 0.02
2.278PheAsp: 2.278 ± 0.042
1.933PheGlu: 1.933 ± 0.044
1.265PhePhe: 1.265 ± 0.042
3.118PheGly: 3.118 ± 0.061
0.677PheHis: 0.677 ± 0.025
1.504PheIle: 1.504 ± 0.044
1.073PheLys: 1.073 ± 0.033
2.832PheLeu: 2.832 ± 0.06
0.773PheMet: 0.773 ± 0.03
0.924PheAsn: 0.924 ± 0.034
1.347PhePro: 1.347 ± 0.038
0.987PheGln: 0.987 ± 0.033
1.69PheArg: 1.69 ± 0.039
1.906PheSer: 1.906 ± 0.044
1.836PheThr: 1.836 ± 0.046
2.493PheVal: 2.493 ± 0.057
0.504PheTrp: 0.504 ± 0.024
0.74PheTyr: 0.74 ± 0.027
0.0PheXaa: 0.0 ± 0.0
Gly
10.585GlyAla: 10.585 ± 0.122
0.931GlyCys: 0.931 ± 0.032
3.888GlyAsp: 3.888 ± 0.067
4.882GlyGlu: 4.882 ± 0.085
3.028GlyPhe: 3.028 ± 0.056
7.024GlyGly: 7.024 ± 0.105
1.968GlyHis: 1.968 ± 0.04
3.636GlyIle: 3.636 ± 0.066
3.08GlyLys: 3.08 ± 0.063
9.11GlyLeu: 9.11 ± 0.104
2.442GlyMet: 2.442 ± 0.052
1.956GlyAsn: 1.956 ± 0.057
3.103GlyPro: 3.103 ± 0.061
4.0GlyGln: 4.0 ± 0.067
5.731GlyArg: 5.731 ± 0.086
4.568GlySer: 4.568 ± 0.066
4.013GlyThr: 4.013 ± 0.071
6.682GlyVal: 6.682 ± 0.079
1.497GlyTrp: 1.497 ± 0.043
2.239GlyTyr: 2.239 ± 0.049
0.0GlyXaa: 0.0 ± 0.0
His
3.282HisAla: 3.282 ± 0.07
0.302HisCys: 0.302 ± 0.017
1.229HisAsp: 1.229 ± 0.034
1.336HisGlu: 1.336 ± 0.041
0.901HisPhe: 0.901 ± 0.031
2.338HisGly: 2.338 ± 0.055
0.63HisHis: 0.63 ± 0.027
0.911HisIle: 0.911 ± 0.028
0.597HisLys: 0.597 ± 0.027
2.358HisLeu: 2.358 ± 0.058
0.54HisMet: 0.54 ± 0.021
0.457HisAsn: 0.457 ± 0.023
1.557HisPro: 1.557 ± 0.044
0.86HisGln: 0.86 ± 0.025
1.395HisArg: 1.395 ± 0.038
1.011HisSer: 1.011 ± 0.038
1.093HisThr: 1.093 ± 0.028
1.537HisVal: 1.537 ± 0.037
0.522HisTrp: 0.522 ± 0.026
0.706HisTyr: 0.706 ± 0.03
0.0HisXaa: 0.0 ± 0.0
Ile
6.134IleAla: 6.134 ± 0.081
0.366IleCys: 0.366 ± 0.019
2.639IleAsp: 2.639 ± 0.051
2.847IleGlu: 2.847 ± 0.061
1.205IlePhe: 1.205 ± 0.042
3.638IleGly: 3.638 ± 0.065
0.855IleHis: 0.855 ± 0.028
1.483IleIle: 1.483 ± 0.047
1.289IleLys: 1.289 ± 0.039
3.224IleLeu: 3.224 ± 0.062
0.748IleMet: 0.748 ± 0.029
1.244IleAsn: 1.244 ± 0.04
1.74IlePro: 1.74 ± 0.049
1.238IleGln: 1.238 ± 0.038
2.392IleArg: 2.392 ± 0.053
2.064IleSer: 2.064 ± 0.047
2.419IleThr: 2.419 ± 0.051
3.415IleVal: 3.415 ± 0.066
0.456IleTrp: 0.456 ± 0.021
0.963IleTyr: 0.963 ± 0.029
0.0IleXaa: 0.0 ± 0.0
Lys
3.658LysAla: 3.658 ± 0.082
0.141LysCys: 0.141 ± 0.012
1.425LysAsp: 1.425 ± 0.042
1.424LysGlu: 1.424 ± 0.046
0.767LysPhe: 0.767 ± 0.03
2.213LysGly: 2.213 ± 0.061
0.617LysHis: 0.617 ± 0.024
1.26LysIle: 1.26 ± 0.035
1.234LysLys: 1.234 ± 0.051
2.997LysLeu: 2.997 ± 0.065
0.736LysMet: 0.736 ± 0.029
0.809LysAsn: 0.809 ± 0.032
1.712LysPro: 1.712 ± 0.044
1.054LysGln: 1.054 ± 0.038
1.902LysArg: 1.902 ± 0.044
1.551LysSer: 1.551 ± 0.041
1.669LysThr: 1.669 ± 0.048
2.206LysVal: 2.206 ± 0.055
0.268LysTrp: 0.268 ± 0.017
0.595LysTyr: 0.595 ± 0.026
0.0LysXaa: 0.0 ± 0.0
Leu
17.812LeuAla: 17.812 ± 0.202
1.063LeuCys: 1.063 ± 0.033
5.672LeuAsp: 5.672 ± 0.088
4.928LeuGlu: 4.928 ± 0.079
3.26LeuPhe: 3.26 ± 0.061
9.198LeuGly: 9.198 ± 0.108
2.664LeuHis: 2.664 ± 0.062
4.114LeuIle: 4.114 ± 0.074
3.279LeuLys: 3.279 ± 0.067
12.672LeuLeu: 12.672 ± 0.192
2.612LeuMet: 2.612 ± 0.051
2.399LeuAsn: 2.399 ± 0.048
6.542LeuPro: 6.542 ± 0.091
5.659LeuGln: 5.659 ± 0.104
8.636LeuArg: 8.636 ± 0.112
5.88LeuSer: 5.88 ± 0.093
5.211LeuThr: 5.211 ± 0.07
8.184LeuVal: 8.184 ± 0.115
1.633LeuTrp: 1.633 ± 0.048
2.112LeuTyr: 2.112 ± 0.045
0.0LeuXaa: 0.0 ± 0.0
Met
3.271MetAla: 3.271 ± 0.059
0.148MetCys: 0.148 ± 0.013
1.177MetAsp: 1.177 ± 0.038
1.041MetGlu: 1.041 ± 0.033
0.693MetPhe: 0.693 ± 0.027
2.092MetGly: 2.092 ± 0.05
0.579MetHis: 0.579 ± 0.025
0.784MetIle: 0.784 ± 0.033
0.872MetLys: 0.872 ± 0.033
2.716MetLeu: 2.716 ± 0.054
0.546MetMet: 0.546 ± 0.025
0.776MetAsn: 0.776 ± 0.028
1.543MetPro: 1.543 ± 0.04
1.489MetGln: 1.489 ± 0.037
1.711MetArg: 1.711 ± 0.037
1.372MetSer: 1.372 ± 0.038
1.319MetThr: 1.319 ± 0.037
1.807MetVal: 1.807 ± 0.044
0.208MetTrp: 0.208 ± 0.014
0.36MetTyr: 0.36 ± 0.018
0.0MetXaa: 0.0 ± 0.0
Asn
3.116AsnAla: 3.116 ± 0.059
0.216AsnCys: 0.216 ± 0.015
1.055AsnAsp: 1.055 ± 0.04
1.029AsnGlu: 1.029 ± 0.037
0.831AsnPhe: 0.831 ± 0.027
1.772AsnGly: 1.772 ± 0.043
0.481AsnHis: 0.481 ± 0.021
1.042AsnIle: 1.042 ± 0.033
0.665AsnLys: 0.665 ± 0.029
2.351AsnLeu: 2.351 ± 0.055
0.521AsnMet: 0.521 ± 0.024
0.56AsnAsn: 0.56 ± 0.025
1.681AsnPro: 1.681 ± 0.045
0.87AsnGln: 0.87 ± 0.03
1.422AsnArg: 1.422 ± 0.04
0.885AsnSer: 0.885 ± 0.032
1.239AsnThr: 1.239 ± 0.041
1.666AsnVal: 1.666 ± 0.047
0.376AsnTrp: 0.376 ± 0.022
0.616AsnTyr: 0.616 ± 0.025
0.0AsnXaa: 0.0 ± 0.0
Pro
7.798ProAla: 7.798 ± 0.137
0.414ProCys: 0.414 ± 0.02
2.895ProAsp: 2.895 ± 0.055
3.692ProGlu: 3.692 ± 0.067
1.535ProPhe: 1.535 ± 0.042
5.061ProGly: 5.061 ± 0.079
1.118ProHis: 1.118 ± 0.033
1.708ProIle: 1.708 ± 0.049
1.195ProLys: 1.195 ± 0.035
5.915ProLeu: 5.915 ± 0.083
1.362ProMet: 1.362 ± 0.035
0.988ProAsn: 0.988 ± 0.031
2.954ProPro: 2.954 ± 0.088
2.378ProGln: 2.378 ± 0.059
3.313ProArg: 3.313 ± 0.066
2.428ProSer: 2.428 ± 0.05
2.347ProThr: 2.347 ± 0.054
4.078ProVal: 4.078 ± 0.064
0.97ProTrp: 0.97 ± 0.032
1.028ProTyr: 1.028 ± 0.036
0.0ProXaa: 0.0 ± 0.0
Gln
7.333GlnAla: 7.333 ± 0.109
0.337GlnCys: 0.337 ± 0.02
2.008GlnAsp: 2.008 ± 0.051
2.131GlnGlu: 2.131 ± 0.052
1.245GlnPhe: 1.245 ± 0.038
3.794GlnGly: 3.794 ± 0.064
1.07GlnHis: 1.07 ± 0.032
1.858GlnIle: 1.858 ± 0.049
1.048GlnLys: 1.048 ± 0.038
5.17GlnLeu: 5.17 ± 0.08
1.157GlnMet: 1.157 ± 0.037
0.798GlnAsn: 0.798 ± 0.03
2.626GlnPro: 2.626 ± 0.055
2.423GlnGln: 2.423 ± 0.059
3.949GlnArg: 3.949 ± 0.071
2.047GlnSer: 2.047 ± 0.047
2.141GlnThr: 2.141 ± 0.046
3.49GlnVal: 3.49 ± 0.064
0.857GlnTrp: 0.857 ± 0.029
0.74GlnTyr: 0.74 ± 0.027
0.0GlnXaa: 0.0 ± 0.0
Arg
9.131ArgAla: 9.131 ± 0.105
0.607ArgCys: 0.607 ± 0.027
3.32ArgAsp: 3.32 ± 0.074
4.602ArgGlu: 4.602 ± 0.088
2.643ArgPhe: 2.643 ± 0.047
5.076ArgGly: 5.076 ± 0.067
2.002ArgHis: 2.002 ± 0.049
3.316ArgIle: 3.316 ± 0.064
2.044ArgLys: 2.044 ± 0.059
8.805ArgLeu: 8.805 ± 0.119
1.992ArgMet: 1.992 ± 0.042
1.57ArgAsn: 1.57 ± 0.034
3.497ArgPro: 3.497 ± 0.065
3.46ArgGln: 3.46 ± 0.06
5.673ArgArg: 5.673 ± 0.083
3.504ArgSer: 3.504 ± 0.062
3.249ArgThr: 3.249 ± 0.055
5.18ArgVal: 5.18 ± 0.075
1.369ArgTrp: 1.369 ± 0.039
1.823ArgTyr: 1.823 ± 0.041
0.0ArgXaa: 0.0 ± 0.0
Ser
6.621SerAla: 6.621 ± 0.081
0.471SerCys: 0.471 ± 0.025
2.429SerAsp: 2.429 ± 0.055
2.546SerGlu: 2.546 ± 0.052
1.855SerPhe: 1.855 ± 0.045
5.132SerGly: 5.132 ± 0.074
1.102SerHis: 1.102 ± 0.032
2.1SerIle: 2.1 ± 0.051
1.229SerLys: 1.229 ± 0.037
5.258SerLeu: 5.258 ± 0.073
1.229SerMet: 1.229 ± 0.034
1.125SerAsn: 1.125 ± 0.035
2.486SerPro: 2.486 ± 0.056
1.775SerGln: 1.775 ± 0.048
3.24SerArg: 3.24 ± 0.056
2.675SerSer: 2.675 ± 0.065
2.644SerThr: 2.644 ± 0.052
3.712SerVal: 3.712 ± 0.072
0.762SerTrp: 0.762 ± 0.026
1.204SerTyr: 1.204 ± 0.036
0.0SerXaa: 0.0 ± 0.0
Thr
5.699ThrAla: 5.699 ± 0.092
0.395ThrCys: 0.395 ± 0.026
2.106ThrAsp: 2.106 ± 0.051
2.335ThrGlu: 2.335 ± 0.051
1.409ThrPhe: 1.409 ± 0.04
4.459ThrGly: 4.459 ± 0.074
1.133ThrHis: 1.133 ± 0.035
1.927ThrIle: 1.927 ± 0.055
1.002ThrLys: 1.002 ± 0.036
6.313ThrLeu: 6.313 ± 0.08
1.053ThrMet: 1.053 ± 0.037
1.056ThrAsn: 1.056 ± 0.042
3.416ThrPro: 3.416 ± 0.058
1.944ThrGln: 1.944 ± 0.042
3.549ThrArg: 3.549 ± 0.059
2.383ThrSer: 2.383 ± 0.053
2.458ThrThr: 2.458 ± 0.063
3.776ThrVal: 3.776 ± 0.07
0.688ThrTrp: 0.688 ± 0.028
0.875ThrTyr: 0.875 ± 0.03
0.0ThrXaa: 0.0 ± 0.0
Val
9.599ValAla: 9.599 ± 0.102
0.748ValCys: 0.748 ± 0.027
3.743ValAsp: 3.743 ± 0.073
3.359ValGlu: 3.359 ± 0.068
2.665ValPhe: 2.665 ± 0.058
5.513ValGly: 5.513 ± 0.075
1.755ValHis: 1.755 ± 0.041
3.033ValIle: 3.033 ± 0.058
2.077ValLys: 2.077 ± 0.052
9.097ValLeu: 9.097 ± 0.114
1.717ValMet: 1.717 ± 0.045
1.941ValAsn: 1.941 ± 0.053
3.792ValPro: 3.792 ± 0.064
3.924ValGln: 3.924 ± 0.067
5.58ValArg: 5.58 ± 0.075
3.648ValSer: 3.648 ± 0.055
3.589ValThr: 3.589 ± 0.08
6.241ValVal: 6.241 ± 0.109
0.965ValTrp: 0.965 ± 0.031
1.474ValTyr: 1.474 ± 0.035
0.0ValXaa: 0.0 ± 0.0
Trp
1.599TrpAla: 1.599 ± 0.047
0.2TrpCys: 0.2 ± 0.013
0.611TrpAsp: 0.611 ± 0.024
0.586TrpGlu: 0.586 ± 0.027
0.555TrpPhe: 0.555 ± 0.024
1.122TrpGly: 1.122 ± 0.039
0.428TrpHis: 0.428 ± 0.02
0.586TrpIle: 0.586 ± 0.025
0.426TrpLys: 0.426 ± 0.02
2.565TrpLeu: 2.565 ± 0.071
0.449TrpMet: 0.449 ± 0.022
0.377TrpAsn: 0.377 ± 0.018
0.848TrpPro: 0.848 ± 0.033
1.019TrpGln: 1.019 ± 0.035
1.35TrpArg: 1.35 ± 0.042
0.876TrpSer: 0.876 ± 0.028
0.622TrpThr: 0.622 ± 0.027
1.024TrpVal: 1.024 ± 0.039
0.36TrpTrp: 0.36 ± 0.023
0.283TrpTyr: 0.283 ± 0.018
0.0TrpXaa: 0.0 ± 0.0
Tyr
2.598TyrAla: 2.598 ± 0.055
0.221TyrCys: 0.221 ± 0.014
1.073TyrAsp: 1.073 ± 0.031
1.148TyrGlu: 1.148 ± 0.032
0.853TyrPhe: 0.853 ± 0.029
1.919TyrGly: 1.919 ± 0.05
0.491TyrHis: 0.491 ± 0.021
0.798TyrIle: 0.798 ± 0.029
0.606TyrLys: 0.606 ± 0.023
2.437TyrLeu: 2.437 ± 0.053
0.432TyrMet: 0.432 ± 0.02
0.543TyrAsn: 0.543 ± 0.024
1.126TyrPro: 1.126 ± 0.036
0.855TyrGln: 0.855 ± 0.029
1.62TyrArg: 1.62 ± 0.039
0.998TyrSer: 0.998 ± 0.036
1.143TyrThr: 1.143 ± 0.034
1.548TyrVal: 1.548 ± 0.042
0.347TyrTrp: 0.347 ± 0.016
0.589TyrTyr: 0.589 ± 0.025
0.0TyrXaa: 0.0 ± 0.0
Xaa
0.0XaaAla: 0.0 ± 0.0
0.0XaaCys: 0.0 ± 0.0
0.0XaaAsp: 0.0 ± 0.0
0.0XaaGlu: 0.0 ± 0.0
0.0XaaPhe: 0.0 ± 0.0
0.0XaaGly: 0.0 ± 0.0
0.0XaaHis: 0.0 ± 0.0
0.0XaaIle: 0.0 ± 0.0
0.0XaaLys: 0.0 ± 0.0
0.0XaaLeu: 0.0 ± 0.0
0.0XaaMet: 0.0 ± 0.0
0.0XaaAsn: 0.0 ± 0.0
0.0XaaPro: 0.0 ± 0.0
0.0XaaGln: 0.0 ± 0.0
0.0XaaArg: 0.0 ± 0.0
0.0XaaSer: 0.0 ± 0.0
0.0XaaThr: 0.0 ± 0.0
0.0XaaVal: 0.0 ± 0.0
0.0XaaTrp: 0.0 ± 0.0
0.0XaaTyr: 0.0 ± 0.0
0.0XaaXaa: 0.0 ± 0.0
Statistics based on 3072 proteins (1003505 amino acids)

Note: The error has been estimated with the bootstraping (x100) at the protein level

Above dipeptide statistics (among other stats for this proteome) you can download from this CSV file
See this proteome in: uniprot_link
Proteome-pI is available under Creative Commons Attribution-NoDerivs license, for more details see here

Reference: Kozlowski LP. Proteome-pI 2.0: Proteome Isoelectric Point Database Update. Nucleic Acids Res. 2021, doi: 10.1093/nar/gkab944 Contact: Lukasz P. Kozlowski