Mycobacterium phage Adjutor

Taxonomy: Viruses; Duplodnaviria; Heunggongvirae; Uroviricota; Caudoviricetes; Caudovirales; Siphoviridae; Dclasvirinae; Plotvirus; Mycobacterium virus Plot

Average proteome isoelectric point is 6.58

Get precalculated fractions of proteins

Acidic
pI < 6.8
6.8-7.4
pI > 7.4
Basic
    
All



Virtual 2D-PAGE plot for 86 proteins (isoelectric point calculated using IPC2_protein)

Get csv file with sequences according to given criteria:
        -     Method 

     -  kDa    
                                                                                      

* You can choose from 21 different methods for calculating isoelectric point

Summary statistics related to proteome-wise predictions


    
Protein with the lowest isoelectric point:
>tr|B2ZNR0|B2ZNR0_9CAUD Uncharacterized protein OS=Mycobacterium phage Adjutor OX=528321 GN=13 PE=4 SV=1
MM1 pKa = 7.48LPVDD5 pKa = 4.6DD6 pKa = 5.88LIEE9 pKa = 4.44HH10 pKa = 6.14VEE12 pKa = 4.96SPDD15 pKa = 3.9CPCDD19 pKa = 3.58PGLEE23 pKa = 4.43LIEE26 pKa = 4.47RR27 pKa = 11.84TDD29 pKa = 3.27DD30 pKa = 4.01HH31 pKa = 6.68FGQMYY36 pKa = 9.54SHH38 pKa = 6.93NAADD42 pKa = 3.72GRR44 pKa = 11.84NDD46 pKa = 3.3EE47 pKa = 5.07AEE49 pKa = 4.19EE50 pKa = 4.01EE51 pKa = 4.36DD52 pKa = 4.84LFF54 pKa = 6.4

Molecular weight: 6.13 kDa
Isoelectric point according different methods:





Protein with the highest isoelectric point:
>tr|B2ZNR1|B2ZNR1_9CAUD Putative head protein OS=Mycobacterium phage Adjutor OX=528321 GN=14 PE=4 SV=1
MM1 pKa = 7.86EE2 pKa = 4.17YY3 pKa = 10.39RR4 pKa = 11.84RR5 pKa = 11.84NTMQSSQQPCLTLRR19 pKa = 11.84KK20 pKa = 8.8MRR22 pKa = 11.84NKK24 pKa = 10.44AYY26 pKa = 10.37AKK28 pKa = 10.56GLATTDD34 pKa = 3.15LDD36 pKa = 5.2RR37 pKa = 11.84RR38 pKa = 11.84LEE40 pKa = 4.79DD41 pKa = 3.32INCSLRR47 pKa = 11.84GLLHH51 pKa = 5.6VTPSSCFSEE60 pKa = 4.44TCIQRR65 pKa = 11.84SQRR68 pKa = 11.84VNRR71 pKa = 11.84RR72 pKa = 11.84QRR74 pKa = 11.84SRR76 pKa = 11.84RR77 pKa = 11.84SKK79 pKa = 10.67QDD81 pKa = 2.74STTIAAFF88 pKa = 3.56

Molecular weight: 10.27 kDa
Isoelectric point according different methods:





Peptides (in silico digests for buttom-up proteomics)

Below you can find in silico digests of the whole proteome with Trypsin, Chymotrypsin, Trypsin+LysC, LysN, ArgC proteases suitable for different mass spec machines.

Try
ESI
ChTry
ESI
ArgC
ESI
LysN
ESI
TryLysC
ESI

Try
MALDI
ChTry
MALDI
ArgC
MALDI
LysN
MALDI
TryLysC
MALDI

Try
LTQ
ChTry
LTQ
ArgC
LTQ
LysN
LTQ
TryLysC
LTQ

Try
MSlow
ChTry
MSlow
ArgC
MSlow
LysN
MSlow
TryLysC
MSlow

Try
MShigh
ChTry
MShigh
ArgC
MShigh
LysN
MShigh
TryLysC
MShigh

General Statistics

Number of major isoforms

Number of additional isoforms

Number of all proteins

Number of amino acids

Min. Seq. Length

Max. Seq. Length

Avg. Seq. Length

Avg. Mol. Weight

86

0

86

20564

37

1590

239.1

26.56

Amino acid frequency

Ala

Cys

Asp

Glu

Phe

Gly

His

Ile

Lys

Leu

9.21 ± 0.393

0.987 ± 0.129

6.881 ± 0.363

6.891 ± 0.256

3.19 ± 0.145

8.262 ± 0.313

1.906 ± 0.16

4.873 ± 0.217

5.534 ± 0.288

7.601 ± 0.198

Met

Asn

Gln

Pro

Arg

Ser

Thr

Val

Trp

Tyr

2.276 ± 0.104

3.924 ± 0.13

5.213 ± 0.184

3.599 ± 0.154

6.599 ± 0.326

5.904 ± 0.237

5.904 ± 0.266

6.22 ± 0.225

1.955 ± 0.121

3.073 ± 0.181
Note: For amino acid frequency statistics the error has been estimated with the bootstraping (x100) at the protein level

Most of the basic statistics you can see at this page can be downloaded from this CSV file

For dipeptide frequency statistics click here
See this proteome in: uniprot_link
Proteome-pI is available under Creative Commons Attribution-NoDerivs license, for more details see here

Reference: Kozlowski LP. Proteome-pI 2.0: Proteome Isoelectric Point Database Update. Nucleic Acids Res. 2021, doi: 10.1093/nar/gkab944 Contact: Lukasz P. Kozlowski