Tropheryma whipplei (strain Twist) (Whipple s bacillus)

Taxonomy: cellular organisms; Bacteria; Terrabacteria group; Actinobacteria; Actinomycetia; Micrococcales; Tropherymataceae; Tropheryma; Tropheryma whipplei

Average proteome isoelectric point is 7.34

Get precalculated fractions of proteins

Acidic
pI < 6.8
6.8-7.4
pI > 7.4
Basic
    
All



Virtual 2D-PAGE plot for 805 proteins (isoelectric point calculated using IPC2_protein)

Get csv file with sequences according to given criteria:
        -     Method 

     -  kDa    
                                                                                      

* You can choose from 21 different methods for calculating isoelectric point

Summary statistics related to proteome-wise predictions


    
Protein with the lowest isoelectric point:
>tr|Q83MT9|Q83MT9_TROWT Mg2+ transporter OS=Tropheryma whipplei (strain Twist) OX=203267 GN=mgtE PE=4 SV=1
MM1 pKa = 7.45TYY3 pKa = 10.61VIAFPCVDD11 pKa = 4.09LKK13 pKa = 11.37DD14 pKa = 3.58RR15 pKa = 11.84ACIDD19 pKa = 3.92EE20 pKa = 4.81CPVDD24 pKa = 4.54CIYY27 pKa = 11.16EE28 pKa = 4.34GGRR31 pKa = 11.84SLYY34 pKa = 10.21INPDD38 pKa = 3.04EE39 pKa = 5.27CVDD42 pKa = 4.03CGACEE47 pKa = 3.85PVCPVEE53 pKa = 4.4AIYY56 pKa = 11.22YY57 pKa = 10.15EE58 pKa = 4.88DD59 pKa = 5.24DD60 pKa = 4.53LPEE63 pKa = 4.05EE64 pKa = 3.64WGEE67 pKa = 3.87YY68 pKa = 9.04YY69 pKa = 10.39RR70 pKa = 11.84ANVEE74 pKa = 4.02FFDD77 pKa = 5.29EE78 pKa = 4.48IGSPGGAAKK87 pKa = 10.5LGPVDD92 pKa = 4.41FDD94 pKa = 4.09HH95 pKa = 7.61PIIAQLPKK103 pKa = 10.7SSDD106 pKa = 3.58SVV108 pKa = 3.37

Molecular weight: 11.94 kDa
Isoelectric point according different methods:





Protein with the highest isoelectric point:
>tr|Q83GJ4|Q83GJ4_TROWT Uncharacterized protein OS=Tropheryma whipplei (strain Twist) OX=203267 GN=TWT_277 PE=4 SV=1
MM1 pKa = 7.42AGSRR5 pKa = 11.84FRR7 pKa = 11.84RR8 pKa = 11.84RR9 pKa = 11.84YY10 pKa = 9.18LLQRR14 pKa = 11.84FHH16 pKa = 6.89RR17 pKa = 11.84TNKK20 pKa = 10.11RR21 pKa = 11.84GGLMSRR27 pKa = 11.84ASAKK31 pKa = 9.91SFRR34 pKa = 11.84FAPAQYY40 pKa = 8.96PCVGVTDD47 pKa = 4.5CLGSPKK53 pKa = 9.95YY54 pKa = 10.63VPSGYY59 pKa = 10.17RR60 pKa = 11.84LVEE63 pKa = 3.92ASVSLGSGDD72 pKa = 3.51KK73 pKa = 10.74RR74 pKa = 11.84FFSSRR79 pKa = 11.84EE80 pKa = 3.59LLMCWKK86 pKa = 9.5VHH88 pKa = 6.09RR89 pKa = 11.84LAGLNVRR96 pKa = 11.84VLDD99 pKa = 4.55PGEE102 pKa = 4.08VDD104 pKa = 4.39CLGGSPILRR113 pKa = 11.84DD114 pKa = 3.34KK115 pKa = 11.18EE116 pKa = 4.29RR117 pKa = 11.84ILDD120 pKa = 3.53EE121 pKa = 4.58SGFDD125 pKa = 3.8FARR128 pKa = 11.84PGCIVALRR136 pKa = 11.84SGLRR140 pKa = 11.84RR141 pKa = 11.84VRR143 pKa = 11.84SVMRR147 pKa = 11.84VVYY150 pKa = 10.2AVSDD154 pKa = 3.85VNRR157 pKa = 11.84VALILASIVGHH168 pKa = 5.63GLHH171 pKa = 6.97LEE173 pKa = 3.83EE174 pKa = 5.24GFFIEE179 pKa = 4.2KK180 pKa = 10.55HH181 pKa = 6.06PDD183 pKa = 2.65SSVRR187 pKa = 11.84FTVRR191 pKa = 11.84ILRR194 pKa = 11.84RR195 pKa = 11.84ASCVSWITMNASKK208 pKa = 10.58HH209 pKa = 4.84LVSRR213 pKa = 11.84YY214 pKa = 9.97LRR216 pKa = 11.84VLDD219 pKa = 4.11PTWALL224 pKa = 3.41

Molecular weight: 25.21 kDa
Isoelectric point according different methods:





Peptides (in silico digests for buttom-up proteomics)

Below you can find in silico digests of the whole proteome with Trypsin, Chymotrypsin, Trypsin+LysC, LysN, ArgC proteases suitable for different mass spec machines.

Try
ESI
ChTry
ESI
ArgC
ESI
LysN
ESI
TryLysC
ESI

Try
MALDI
ChTry
MALDI
ArgC
MALDI
LysN
MALDI
TryLysC
MALDI

Try
LTQ
ChTry
LTQ
ArgC
LTQ
LysN
LTQ
TryLysC
LTQ

Try
MSlow
ChTry
MSlow
ArgC
MSlow
LysN
MSlow
TryLysC
MSlow

Try
MShigh
ChTry
MShigh
ArgC
MShigh
LysN
MShigh
TryLysC
MShigh

General Statistics

Number of major isoforms

Number of additional isoforms

Number of all proteins

Number of amino acids

Min. Seq. Length

Max. Seq. Length

Avg. Seq. Length

Avg. Mol. Weight

805

0

805

263585

37

2312

327.4

36.05

Amino acid frequency

Ala

Cys

Asp

Glu

Phe

Gly

His

Ile

Lys

Leu

8.332 ± 0.1

1.336 ± 0.036

5.269 ± 0.076

5.022 ± 0.088

4.207 ± 0.066

7.367 ± 0.073

2.081 ± 0.041

6.741 ± 0.072

4.75 ± 0.062

10.263 ± 0.082

Met

Asn

Gln

Pro

Arg

Ser

Thr

Val

Trp

Tyr

1.982 ± 0.036

3.578 ± 0.042

4.561 ± 0.068

3.091 ± 0.042

6.291 ± 0.074

7.915 ± 0.065

5.473 ± 0.131

7.945 ± 0.082

1.016 ± 0.033

2.778 ± 0.051
Note: For amino acid frequency statistics the error has been estimated with the bootstraping (x100) at the protein level

Most of the basic statistics you can see at this page can be downloaded from this CSV file

For dipeptide frequency statistics click here
See this proteome in: uniprot_link
Proteome-pI is available under Creative Commons Attribution-NoDerivs license, for more details see here

Reference: Kozlowski LP. Proteome-pI 2.0: Proteome Isoelectric Point Database Update. Nucleic Acids Res. 2021, doi: 10.1093/nar/gkab944 Contact: Lukasz P. Kozlowski