Thiorhodococcus drewsii AZ1

Taxonomy: cellular organisms; Bacteria; Proteobacteria; Gammaproteobacteria; Chromatiales; Chromatiaceae; Thiorhodococcus; Thiorhodococcus drewsii

Average proteome isoelectric point is 6.23

Get precalculated fractions of proteins

Acidic
pI < 6.8
6.8-7.4
pI > 7.4
Basic
    
All



Virtual 2D-PAGE plot for 4731 proteins (isoelectric point calculated using IPC2_protein)

Get csv file with sequences according to given criteria:
        -     Method 

     -  kDa    
                                                                                      

* You can choose from 21 different methods for calculating isoelectric point

Summary statistics related to proteome-wise predictions


    
Protein with the lowest isoelectric point:
>tr|G2E7E9|G2E7E9_9GAMM Methyltransferase type 11 OS=Thiorhodococcus drewsii AZ1 OX=765913 GN=ThidrDRAFT_4212 PE=4 SV=1
MM1 pKa = 7.94IIYY4 pKa = 9.54TDD6 pKa = 4.37LAQPLLDD13 pKa = 3.41YY14 pKa = 11.1VSAVEE19 pKa = 4.68AEE21 pKa = 4.13ASGTSDD27 pKa = 3.77SLNVDD32 pKa = 4.0LTLSADD38 pKa = 4.43FFAKK42 pKa = 10.31RR43 pKa = 11.84PQILTANFGFDD54 pKa = 3.18GYY56 pKa = 10.17MGVPGLVGADD66 pKa = 3.23ATAQSAAYY74 pKa = 8.63QFNVAWEE81 pKa = 4.28ALDD84 pKa = 3.99PALGAATRR92 pKa = 11.84AYY94 pKa = 10.07YY95 pKa = 10.54SAVGPAAFLSVYY107 pKa = 9.78GVPALLADD115 pKa = 4.87TIPVVFSHH123 pKa = 6.88PVLGEE128 pKa = 4.01TVNPEE133 pKa = 4.05DD134 pKa = 5.25FLVTLNTGEE143 pKa = 4.24VVTPITASFLPNLEE157 pKa = 3.99FNEE160 pKa = 4.14RR161 pKa = 11.84QTVVISGEE169 pKa = 3.75WGNRR173 pKa = 11.84IAPGEE178 pKa = 4.07PGARR182 pKa = 11.84YY183 pKa = 8.21PVTVSIVDD191 pKa = 4.05DD192 pKa = 4.59GSPLCFVTDD201 pKa = 3.44TGLISAVGLSVEE213 pKa = 4.28SQNPYY218 pKa = 10.27VEE220 pKa = 4.87GNGPRR225 pKa = 11.84ILAAKK230 pKa = 10.11LNAFSDD236 pKa = 3.91LGEE239 pKa = 5.08GSPAWQGSSTANSGADD255 pKa = 3.51LYY257 pKa = 11.38GDD259 pKa = 3.17QAMYY263 pKa = 10.8RR264 pKa = 11.84LRR266 pKa = 11.84IYY268 pKa = 10.14TSAGFSPDD276 pKa = 3.58GIASISPDD284 pKa = 3.18DD285 pKa = 3.85FSRR288 pKa = 11.84FFQLTALDD296 pKa = 3.85QGGHH300 pKa = 5.62EE301 pKa = 4.69LLLLEE306 pKa = 4.57TGVDD310 pKa = 3.94YY311 pKa = 11.35VISGFGAVRR320 pKa = 11.84ILGIADD326 pKa = 3.4TGLVQDD332 pKa = 5.13SYY334 pKa = 10.82NAAYY338 pKa = 9.7IEE340 pKa = 4.38DD341 pKa = 4.6HH342 pKa = 7.25DD343 pKa = 4.12NQYY346 pKa = 11.49DD347 pKa = 3.84IILSGDD353 pKa = 3.32LAAIEE358 pKa = 4.36RR359 pKa = 11.84LQSVRR364 pKa = 11.84MPSGDD369 pKa = 3.34GYY371 pKa = 11.39SPVYY375 pKa = 10.75NPGGPGNDD383 pKa = 4.03PDD385 pKa = 4.55NNPPGPFTVPSSDD398 pKa = 3.15QTVDD402 pKa = 2.7ITHH405 pKa = 7.6DD406 pKa = 3.54FTAASFVSYY415 pKa = 11.08VEE417 pKa = 3.99VDD419 pKa = 3.61GQVVHH424 pKa = 6.55HH425 pKa = 7.55AITGQPIGVDD435 pKa = 2.86QGLAVEE441 pKa = 4.66DD442 pKa = 4.17TSTGHH447 pKa = 7.59KK448 pKa = 9.26INQYY452 pKa = 9.95IDD454 pKa = 3.39PDD456 pKa = 3.76GKK458 pKa = 10.09IFYY461 pKa = 10.46SSFEE465 pKa = 3.69VSPIYY470 pKa = 9.6EE471 pKa = 3.69IHH473 pKa = 5.83LTEE476 pKa = 4.4VQPSTYY482 pKa = 10.75SRR484 pKa = 11.84VTDD487 pKa = 3.84DD488 pKa = 4.47QITGSAQIDD497 pKa = 3.29RR498 pKa = 11.84VSFSGAFDD506 pKa = 3.24QYY508 pKa = 11.24VRR510 pKa = 11.84SGNLAEE516 pKa = 5.71FSSQDD521 pKa = 3.69LVPLRR526 pKa = 11.84DD527 pKa = 3.56ADD529 pKa = 4.65DD530 pKa = 3.83NLFDD534 pKa = 4.14VEE536 pKa = 4.24RR537 pKa = 11.84LIYY540 pKa = 10.47TDD542 pKa = 3.14TTLALDD548 pKa = 3.91IEE550 pKa = 4.74GHH552 pKa = 6.48AGFAYY557 pKa = 10.3SLYY560 pKa = 10.54GVFGRR565 pKa = 11.84APDD568 pKa = 3.66AAGLGDD574 pKa = 5.07WINALDD580 pKa = 4.26NGFSLQSVADD590 pKa = 4.21RR591 pKa = 11.84FLASPEE597 pKa = 4.17FKK599 pKa = 9.29THH601 pKa = 6.23YY602 pKa = 10.37GPPLTNGSYY611 pKa = 10.44VEE613 pKa = 3.79QLYY616 pKa = 11.12EE617 pKa = 3.93NFLNRR622 pKa = 11.84SAEE625 pKa = 3.97ASGYY629 pKa = 8.86DD630 pKa = 3.35AWVGALDD637 pKa = 4.7AGLLDD642 pKa = 4.36RR643 pKa = 11.84SDD645 pKa = 3.65VLIAFSRR652 pKa = 11.84SPEE655 pKa = 4.01YY656 pKa = 10.4QDD658 pKa = 6.05LIAGDD663 pKa = 3.64IANGIRR669 pKa = 11.84FDD671 pKa = 4.52LWWQQ675 pKa = 3.28

Molecular weight: 72.29 kDa
Isoelectric point according different methods:





Protein with the highest isoelectric point:
>tr|G2DXC3|G2DXC3_9GAMM Uncharacterized protein OS=Thiorhodococcus drewsii AZ1 OX=765913 GN=ThidrDRAFT_0684 PE=4 SV=1
MM1 pKa = 7.36RR2 pKa = 11.84QVPLEE7 pKa = 4.26SNSARR12 pKa = 11.84EE13 pKa = 4.22PKK15 pKa = 10.39VGAGVVGRR23 pKa = 11.84SVGGLRR29 pKa = 11.84LFASCASFVLLCLVLARR46 pKa = 11.84SHH48 pKa = 6.66AEE50 pKa = 3.73SFSEE54 pKa = 4.33VEE56 pKa = 3.93VRR58 pKa = 11.84AVYY61 pKa = 10.38LFNFAVFVDD70 pKa = 4.33WPPTAFEE77 pKa = 4.88SSTSPIRR84 pKa = 11.84YY85 pKa = 8.82CVVGSQALRR94 pKa = 11.84ASLEE98 pKa = 3.95EE99 pKa = 4.09VLADD103 pKa = 3.38EE104 pKa = 4.74HH105 pKa = 7.66VGGRR109 pKa = 11.84ALRR112 pKa = 11.84LISANEE118 pKa = 3.61PLDD121 pKa = 3.12WRR123 pKa = 11.84RR124 pKa = 11.84CHH126 pKa = 6.98LLYY129 pKa = 10.26IGHH132 pKa = 6.39GAKK135 pKa = 9.0TRR137 pKa = 11.84ARR139 pKa = 11.84QILKK143 pKa = 10.06AVRR146 pKa = 11.84GTAVLTIGASEE157 pKa = 4.11AFVRR161 pKa = 11.84EE162 pKa = 3.85GGMIGLVRR170 pKa = 11.84RR171 pKa = 11.84GQRR174 pKa = 11.84LRR176 pKa = 11.84TIIDD180 pKa = 2.89RR181 pKa = 11.84DD182 pKa = 3.15AAEE185 pKa = 3.94RR186 pKa = 11.84VGIRR190 pKa = 11.84ISSKK194 pKa = 10.24LLRR197 pKa = 11.84LSTLVSGTRR206 pKa = 11.84EE207 pKa = 4.13GVSPP211 pKa = 4.5

Molecular weight: 22.94 kDa
Isoelectric point according different methods:





Peptides (in silico digests for buttom-up proteomics)

Below you can find in silico digests of the whole proteome with Trypsin, Chymotrypsin, Trypsin+LysC, LysN, ArgC proteases suitable for different mass spec machines.

Try
ESI
ChTry
ESI
ArgC
ESI
LysN
ESI
TryLysC
ESI

Try
MALDI
ChTry
MALDI
ArgC
MALDI
LysN
MALDI
TryLysC
MALDI

Try
LTQ
ChTry
LTQ
ArgC
LTQ
LysN
LTQ
TryLysC
LTQ

Try
MSlow
ChTry
MSlow
ArgC
MSlow
LysN
MSlow
TryLysC
MSlow

Try
MShigh
ChTry
MShigh
ArgC
MShigh
LysN
MShigh
TryLysC
MShigh

General Statistics

Number of major isoforms

Number of additional isoforms

Number of all proteins

Number of amino acids

Min. Seq. Length

Max. Seq. Length

Avg. Seq. Length

Avg. Mol. Weight

4731

0

4731

1618336

30

4605

342.1

37.66

Amino acid frequency

Ala

Cys

Asp

Glu

Phe

Gly

His

Ile

Lys

Leu

10.771 ± 0.041

1.023 ± 0.013

6.068 ± 0.03

6.345 ± 0.033

3.473 ± 0.02

7.864 ± 0.036

2.254 ± 0.017

4.992 ± 0.026

2.772 ± 0.025

11.415 ± 0.047

Met

Asn

Gln

Pro

Arg

Ser

Thr

Val

Trp

Tyr

2.211 ± 0.018

2.564 ± 0.017

5.128 ± 0.029

3.803 ± 0.025

7.771 ± 0.042

5.815 ± 0.026

5.094 ± 0.031

6.826 ± 0.03

1.439 ± 0.016

2.374 ± 0.021
Note: For amino acid frequency statistics the error has been estimated with the bootstraping (x100) at the protein level

Most of the basic statistics you can see at this page can be downloaded from this CSV file

For dipeptide frequency statistics click here
See this proteome in: uniprot_link
Proteome-pI is available under Creative Commons Attribution-NoDerivs license, for more details see here

Reference: Kozlowski LP. Proteome-pI 2.0: Proteome Isoelectric Point Database Update. Nucleic Acids Res. 2021, doi: 10.1093/nar/gkab944 Contact: Lukasz P. Kozlowski