Nitrosopumilus sp. PRT-SC01

Taxonomy: cellular organisms; Archaea; TACK group; Thaumarchaeota; Nitrosopumilales; Nitrosopumilaceae; Nitrosopumilus; unclassified Nitrosopumilus

Average proteome isoelectric point is 6.75

Get precalculated fractions of proteins

Acidic
pI < 6.8
6.8-7.4
pI > 7.4
Basic
    
All



Virtual 2D-PAGE plot for 709 proteins (isoelectric point calculated using IPC2_protein)

Get csv file with sequences according to given criteria:
        -     Method 

     -  kDa    
                                                                                      

* You can choose from 21 different methods for calculating isoelectric point

Summary statistics related to proteome-wise predictions


    
Protein with the lowest isoelectric point:
>tr|A0A0P9CEV8|A0A0P9CEV8_9ARCH 50S ribosomal protein L10e OS=Nitrosopumilus sp. PRT-SC01 OX=1527301 GN=JI55_00745 PE=4 SV=1
MM1 pKa = 7.69RR2 pKa = 11.84PIGLIVVAVSLGAIMAIIAGDD23 pKa = 3.74YY24 pKa = 10.62SNQDD28 pKa = 3.22EE29 pKa = 4.89APWDD33 pKa = 3.74GLNCDD38 pKa = 3.63EE39 pKa = 5.09MLDD42 pKa = 4.16FSASDD47 pKa = 3.4EE48 pKa = 4.24HH49 pKa = 9.19SLMEE53 pKa = 5.17DD54 pKa = 3.15SMHH57 pKa = 6.38MEE59 pKa = 3.89FHH61 pKa = 7.09EE62 pKa = 5.63DD63 pKa = 4.03YY64 pKa = 10.84INHH67 pKa = 6.59CSDD70 pKa = 3.46TEE72 pKa = 4.09IPP74 pKa = 3.84

Molecular weight: 8.25 kDa
Isoelectric point according different methods:





Protein with the highest isoelectric point:
>tr|A0A0P9A5I2|A0A0P9A5I2_9ARCH 6 7-dimethyl-8-ribityllumazine synthase OS=Nitrosopumilus sp. PRT-SC01 OX=1527301 GN=JI55_00035 PE=3 SV=1
MM1 pKa = 7.79AISKK5 pKa = 10.28AKK7 pKa = 9.98RR8 pKa = 11.84AAAAQKK14 pKa = 10.22AARR17 pKa = 11.84TRR19 pKa = 11.84KK20 pKa = 9.65RR21 pKa = 11.84NAAAKK26 pKa = 9.86AAAATKK32 pKa = 10.38AKK34 pKa = 9.42TSRR37 pKa = 11.84RR38 pKa = 11.84RR39 pKa = 11.84KK40 pKa = 8.92AAATRR45 pKa = 11.84KK46 pKa = 8.9VAAPKK51 pKa = 10.16RR52 pKa = 11.84RR53 pKa = 11.84AAPKK57 pKa = 9.92RR58 pKa = 11.84RR59 pKa = 11.84AAPKK63 pKa = 9.28RR64 pKa = 11.84KK65 pKa = 9.35AVNRR69 pKa = 11.84RR70 pKa = 11.84AAPKK74 pKa = 9.89RR75 pKa = 11.84KK76 pKa = 8.68AAKK79 pKa = 9.69RR80 pKa = 11.84RR81 pKa = 11.84AAPKK85 pKa = 9.8RR86 pKa = 11.84KK87 pKa = 9.06AANRR91 pKa = 11.84RR92 pKa = 11.84AAPKK96 pKa = 10.0RR97 pKa = 11.84KK98 pKa = 8.82AAKK101 pKa = 8.41RR102 pKa = 11.84TRR104 pKa = 3.3

Molecular weight: 11.33 kDa
Isoelectric point according different methods:





Peptides (in silico digests for buttom-up proteomics)

Below you can find in silico digests of the whole proteome with Trypsin, Chymotrypsin, Trypsin+LysC, LysN, ArgC proteases suitable for different mass spec machines.

Try
ESI
ChTry
ESI
ArgC
ESI
LysN
ESI
TryLysC
ESI

Try
MALDI
ChTry
MALDI
ArgC
MALDI
LysN
MALDI
TryLysC
MALDI

Try
LTQ
ChTry
LTQ
ArgC
LTQ
LysN
LTQ
TryLysC
LTQ

Try
MSlow
ChTry
MSlow
ArgC
MSlow
LysN
MSlow
TryLysC
MSlow

Try
MShigh
ChTry
MShigh
ArgC
MShigh
LysN
MShigh
TryLysC
MShigh

General Statistics

Number of major isoforms

Number of additional isoforms

Number of all proteins

Number of amino acids

Min. Seq. Length

Max. Seq. Length

Avg. Seq. Length

Avg. Mol. Weight

709

0

709

158763

34

1263

223.9

25.14

Amino acid frequency

Ala

Cys

Asp

Glu

Phe

Gly

His

Ile

Lys

Leu

6.008 ± 0.112

1.055 ± 0.038

5.532 ± 0.07

7.118 ± 0.083

4.319 ± 0.07

6.553 ± 0.087

1.774 ± 0.039

9.305 ± 0.091

9.08 ± 0.124

8.68 ± 0.088

Met

Asn

Gln

Pro

Arg

Ser

Thr

Val

Trp

Tyr

2.63 ± 0.044

4.858 ± 0.066

3.549 ± 0.051

3.09 ± 0.048

3.691 ± 0.077

6.962 ± 0.078

5.414 ± 0.071

6.393 ± 0.078

0.856 ± 0.034

3.135 ± 0.061
Note: For amino acid frequency statistics the error has been estimated with the bootstraping (x100) at the protein level

Most of the basic statistics you can see at this page can be downloaded from this CSV file

For dipeptide frequency statistics click here
See this proteome in: uniprot_link
Proteome-pI is available under Creative Commons Attribution-NoDerivs license, for more details see here

Reference: Kozlowski LP. Proteome-pI 2.0: Proteome Isoelectric Point Database Update. Nucleic Acids Res. 2021, doi: 10.1093/nar/gkab944 Contact: Lukasz P. Kozlowski