Desulfoplanes formicivorans
Average proteome isoelectric point is 6.72
Get precalculated fractions of proteins
Acidic
pI < 6.8
6.8-7.4
pI > 7.4
Basic
All
Note: above files contain also dissociation constants (pKa)
Virtual 2D-PAGE plot for 2657 proteins (isoelectric point calculated using IPC2_protein)
Get csv file with sequences according to given criteria:
* You can choose from 21 different methods for calculating isoelectric point
Summary statistics related to proteome-wise predictions
Protein with the lowest isoelectric point:
>tr|A0A194AG35|A0A194AG35_9DELT FMN reductase OS=Desulfoplanes formicivorans OX=1592317 GN=DPF_0437 PE=4 SV=1
MM1 pKa = 7.62 RR2 pKa = 11.84 VIEE5 pKa = 5.18 PIDD8 pKa = 3.46 IEE10 pKa = 5.51 LIDD13 pKa = 4.46 SNVTQSGLSEE23 pKa = 3.89 WDD25 pKa = 3.17 AEE27 pKa = 4.43 TIYY30 pKa = 11.05 AAGQRR35 pKa = 11.84 VVVSYY40 pKa = 11.03 EE41 pKa = 3.94 EE42 pKa = 5.21 DD43 pKa = 3.82 GEE45 pKa = 4.56 TPRR48 pKa = 11.84 VAEE51 pKa = 4.81 EE52 pKa = 4.05 YY53 pKa = 10.22 TSLRR57 pKa = 11.84 SDD59 pKa = 2.94 NVGFYY64 pKa = 10.54 PPDD67 pKa = 3.49 SSGDD71 pKa = 3.53 GQDD74 pKa = 3.24 GVKK77 pKa = 10.2 ISVGDD82 pKa = 3.97 SEE84 pKa = 5.41 DD85 pKa = 3.41 VSSFTIGEE93 pKa = 4.46 TVGTSLASGTCSFIDD108 pKa = 4.02 GTTGGYY114 pKa = 8.97 MILSGVSGDD123 pKa = 3.96 FDD125 pKa = 4.45 SGDD128 pKa = 3.83 TISGNDD134 pKa = 3.31 SGASATISSVSSTTIAAAWEE154 pKa = 4.17 RR155 pKa = 11.84 VGATNRR161 pKa = 11.84 WKK163 pKa = 10.61 MFDD166 pKa = 3.78 AYY168 pKa = 11.39 VNSQTVADD176 pKa = 5.57 DD177 pKa = 3.38 IDD179 pKa = 3.77 VTIGFGRR186 pKa = 11.84 CDD188 pKa = 3.62 TLVLLDD194 pKa = 3.77 VQATDD199 pKa = 3.67 VEE201 pKa = 4.53 IEE203 pKa = 4.29 VVNDD207 pKa = 3.29 STGEE211 pKa = 4.23 VVFSEE216 pKa = 4.46 AFDD219 pKa = 3.62 MRR221 pKa = 11.84 LDD223 pKa = 4.32 QSASWADD230 pKa = 3.71 YY231 pKa = 9.54 FFAPAEE237 pKa = 4.35 YY238 pKa = 8.09 KK239 pKa = 10.23 TSLIVSTPLYY249 pKa = 9.28 YY250 pKa = 10.39 NASARR255 pKa = 11.84 IRR257 pKa = 11.84 ITNGDD262 pKa = 3.84 DD263 pKa = 3.19 EE264 pKa = 5.31 AMCGHH269 pKa = 6.23 VVAGRR274 pKa = 11.84 AQYY277 pKa = 10.15 IAASQWSPTAGITDD291 pKa = 3.72 YY292 pKa = 11.56 SSAEE296 pKa = 3.94 TDD298 pKa = 3.18 TDD300 pKa = 3.76 GVTSLEE306 pKa = 3.78 QGPWSKK312 pKa = 10.64 EE313 pKa = 3.62 LSVDD317 pKa = 3.79 LLVPTASVSNLQRR330 pKa = 11.84 RR331 pKa = 11.84 LASYY335 pKa = 10.13 RR336 pKa = 11.84 GKK338 pKa = 9.96 PCVWDD343 pKa = 4.1 CNNDD347 pKa = 3.78 STDD350 pKa = 3.17 HH351 pKa = 7.16 DD352 pKa = 4.01 ALIVYY357 pKa = 8.86 GYY359 pKa = 10.36 FSDD362 pKa = 4.49 FSIVIAGPTTSSCSLTIQGLII383 pKa = 3.77
Molecular weight: 40.84 kDa
Isoelectric point according different methods:
IPC2.protein.svr19 3.684
IPC2_protein 3.77
IPC_protein 3.795
Toseland 3.567
ProMoST 3.961
Dawson 3.795
Bjellqvist 3.948
Wikipedia 3.732
Rodwell 3.617
Grimsley 3.478
Solomon 3.783
Lehninger 3.745
Nozaki 3.897
DTASelect 4.151
Thurlkill 3.617
EMBOSS 3.732
Sillero 3.923
Patrickios 1.303
IPC_peptide 3.783
IPC2_peptide 3.897
IPC2.peptide.svr19 3.822
Protein with the highest isoelectric point:
>tr|A0A194AHT9|A0A194AHT9_9DELT Uncharacterized protein OS=Desulfoplanes formicivorans OX=1592317 GN=DPF_1045 PE=4 SV=1
MM1 pKa = 7.86 PKK3 pKa = 10.65 LKK5 pKa = 9.79 TNKK8 pKa = 9.61 SAAKK12 pKa = 10.15 RR13 pKa = 11.84 FTTTGTGKK21 pKa = 10.25 VKK23 pKa = 10.65 RR24 pKa = 11.84 NRR26 pKa = 11.84 SNSQHH31 pKa = 6.67 ILTKK35 pKa = 10.63 KK36 pKa = 7.69 NAKK39 pKa = 9.18 RR40 pKa = 11.84 KK41 pKa = 9.01 RR42 pKa = 11.84 RR43 pKa = 11.84 LRR45 pKa = 11.84 QSTTMDD51 pKa = 3.01 KK52 pKa = 10.13 STLKK56 pKa = 10.26 SVKK59 pKa = 9.98 RR60 pKa = 11.84 LVPTIFF66 pKa = 3.73
Molecular weight: 7.6 kDa
Isoelectric point according different methods:
IPC2.protein.svr19 9.359
IPC2_protein 10.804
IPC_protein 12.325
Toseland 12.515
ProMoST 12.983
Dawson 12.515
Bjellqvist 12.486
Wikipedia 12.969
Rodwell 12.471
Grimsley 12.544
Solomon 12.983
Lehninger 12.896
Nozaki 12.501
DTASelect 12.486
Thurlkill 12.501
EMBOSS 12.998
Sillero 12.501
Patrickios 12.193
IPC_peptide 12.998
IPC2_peptide 11.974
IPC2.peptide.svr19 8.978
Peptides (in silico digests for buttom-up proteomics)
Below you can find
in silico digests of the whole proteome with Trypsin, Chymotrypsin, Trypsin+LysC, LysN, ArgC proteases suitable for different mass spec machines.
Try ESI
ChTry ESI
ArgC ESI
LysN ESI
TryLysC ESI
Try MALDI
ChTry MALDI
ArgC MALDI
LysN MALDI
TryLysC MALDI
Try LTQ
ChTry LTQ
ArgC LTQ
LysN LTQ
TryLysC LTQ
Try MSlow
ChTry MSlow
ArgC MSlow
LysN MSlow
TryLysC MSlow
Try MShigh
ChTry MShigh
ArgC MShigh
LysN MShigh
TryLysC MShigh
General Statistics
Number of major isoforms
Number of additional isoforms
Number of all proteins
Number of amino acids
Min. Seq. Length
Max. Seq. Length
Avg. Seq. Length
Avg. Mol. Weight
2657
0
2657
859764
41
5430
323.6
35.92
Amino acid frequency
Ala
Cys
Asp
Glu
Phe
Gly
His
Ile
Lys
Leu
8.662 ± 0.051
1.423 ± 0.021
5.498 ± 0.037
5.819 ± 0.05
4.164 ± 0.031
7.634 ± 0.039
2.435 ± 0.025
6.18 ± 0.05
4.932 ± 0.047
10.49 ± 0.059
Met
Asn
Gln
Pro
Arg
Ser
Thr
Val
Trp
Tyr
2.94 ± 0.02
3.222 ± 0.028
4.735 ± 0.036
3.85 ± 0.027
5.896 ± 0.037
5.543 ± 0.037
5.495 ± 0.032
7.122 ± 0.044
1.232 ± 0.02
2.728 ± 0.026
Note: For amino acid frequency statistics the error has been estimated with the bootstraping (x100) at the protein level
Most of the basic statistics you can see at this page can be downloaded from this CSV file
For dipeptide frequency statistics click here